Learn MPO in 3 minutes | Myeloperoxidase
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Does your next experiment involve Myeloperoxidase (MPO)? This is a presentation about MPO intended for scientists who are designing controls and performing immunoassays detecting MPO. It contains useful info such as Western blot band size, protein expression, and interesting facts. Anti-Myeloperoxidase MPO Antibody (A00372): https://www.bosterbio.com/anti-myeloperoxidase-antibody-a00372-boster.html References: Uniprot.org, ProteinAtlas.org, PMC4121192, PMC4921022, PMC4115493 Learn more about MPO (infographic and discussion): https://www.bosterbio.com/bosterbio-gene-info-cards/MPO Boster Biological Technology Website: www.bosterbio.com Email: support@bosterbio.com
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Learn MPO in 3 minutes | Myeloperoxidase
- 1. www.bosterbio.com MPO IN 3 MINUTES Quick facts, control designs, Western Blot MW. Info you can use to find the best MPO antibody.
- 2. FACTS 01. Function, structure, etc. TISSUES 02. Expressing tissues/cells WESTERN BLOT 03. Expected band sizes HISTORY 04. …and medical significance TABLE OF CONTENTS
- 3. ● Myeloperoxidase (MPO) ● Catalyzes the formation of HClO: ○ H+ + Cl- + H2O2 → HClO + H2O ● Has microbicidal activity against a wide range of microorganisms ● Present in polymorphonuclear leukocytes ● Heterodimeric: one 𝝰 and one 𝛃 subunit ● 𝝰 subunit: ~55 kDa ● 𝛃 subunit: ~15 kDa ● Entire protein: ~150 kDa 01. FACTS AlphaFold predicted structure Image source: https://alphafold.ebi.ac.uk/entry/P05164
- 4. 02. TISSUES Image source: https://www.proteinatlas.org/ENSG00000005381-MPO/tissue
- 5. 03. MPO WESTERN BLOT 488 images available on PMC: https://www.ncbi.nlm.nih.gov/pmc/?term=MPO+western +blot&report=imagesdocsum After reviewing several WB images, we observe that MPO has prominent bands at ~150 kDa and ~65 kDa. PMC4121192 Figure 4E Abcam Anti-Myeloperoxidase antibody (ab134132) PMC4921022 Figure 5B Boster Bio Anti- MPO Antibody (A00372, Rabbit polyclonal) PMC4115493 Figure 2C
- 6. Chlorinating activity of MPO was first studied. 04. HISTORY MPO has a role in the pathogenesis of atherosclerotic coronary artery disease (CAD), and serves as a potential therapeutic target in CAD. 1976 CLINICAL POTENTIAL
- 7. 3942 B Valley Ave Pleasanton, CA 94566 Tel: (888) 466-3604 Email: support@bosterbio.com www.bosterbio.com
Editor's Notes
- Does your next experiment involve Myeloperoxidase? This video is for scientists who want to run Western blot, IHC, flow or other immunoassays against MPO.
- We will cover some basic facts, protein expression, and MPO’s expected behavior in Western blot. All information in this video is based on public information and no proprietary experimental evidence was used.
- Topic 1: Quick facts about MPO. Myeloperoxidase, or MPO, is a heterodimeric protein with an alpha and a beta subunit. It catalyzes the conversion of hydrogen ions, chloride ions, and hydrogen peroxide into hypochlorous acid. This confers microbicidal activity to MPO against a wide variety of microorganisms. Myeloperoxidase is mostly expressed in polymorphonuclear leukocytes, which are cells of the immune system. MPO’s molecular weight is approximately 150 kilodaltons. The heavy chain, or the alpha subunit, is 55 kilodaltons while the light chain, or the beta subunit, is only 15 kilodaltons.
- Topic 2: Where is MPO expressed? Myeloperoxidase is only present in tissues that produce immune cells such as neutrophils. High levels of MPO have been found in the bone marrow, lymph nodes, and other lymphoid tissues. Other tissue types do not express MPO. You can find more information on protein atlas dot org to check out which cell lines express MPO and which ones don’t. This information will come in handy when designing positive and negative controls for western blot and immunohistochemistry.
- Topic 3: MPO in Western blotting. Antibody specificity can make or break an experiment. After looking through Western blot images from publications and antibody companies, we can see that MPO has distinct bands in the 140-150 kilodalton range. Another band is also detected around 60 to 65 kilodaltons, representing the MPO heavy chain. Check out the PMC link for more images, which is also provided in the description box.
- Topic 4: Some interesting facts about Myeloperoxidase. The chlorinating activity of MPO was first discovered by researchers in 1976. This was shown to contribute to MPO’s antimicrobial activity. In 2020, MPO was found to play a role in the progression of atherosclerotic coronary artery disease or CAD. Therefore, MPO presents a possible target for the treatment of CAD.