Learn CTSB in 3 minutes | Cathepsin B
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Does your next experiment involve Cathepsin B (CTSB)? This is a presentation about CTSB intended for scientists who are designing controls and performing immunoassays detecting CTSB. It contains useful info such as Western blot band size, protein expression, and interesting facts. Anti-Cathepsin B CTSB Rabbit Monoclonal Antibody (M01456): https://www.bosterbio.com/anti-cathepsin-b-rabbit-monoclonal-antibody-m01456-boster.html References: Uniprot.org, ProteinAtlas.org, PMC6351122, PMC4091172, PMC7391525 Learn more about CTSB (infographic and discussion): https://www.bosterbio.com/bosterbio-gene-info-cards/CTSB Boster Biological Technology Website: www.bosterbio.com Email: support@bosterbio.com
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Learn CTSB in 3 minutes | Cathepsin B
- 1. www.bosterbio.com CTSB IN 3 MINUTES Quick facts, control designs, Western Blot MW. Info you can use to find the best CTSB antibody.
- 2. FACTS 01. Function, structure, etc. TISSUES 02. Expressing tissues/cells WESTERN BLOT 03. Expected band sizes HISTORY 04. …and medical significance TABLE OF CONTENTS
- 3. ● Cathepsin B (CTSB) ● Bilobal protein ○ Catalytic site is located at interface between the 2 lobes ● Precursor/pro-form ○ 339 AAs, ~35 kDa ● Mature form ○ 254 AAs, ~25 kDa 01. FACTS AlphaFold predicted structure Image source: https://alphafold.ebi.ac.uk/entry/P07858
- 4. 02. TISSUES Image source: https://www.proteinatlas.org/ENSG00000164733-CTSB/tissue
- 5. 03. CTSB WESTERN BLOT PMC6351122 Figure 1 PMC4091172 Figure 4 Boster Bio CTSB Antibody (M01456, Rabbit monoclonal) 161 images available on PMC: https://www.ncbi.nlm.nih.gov/pmc/?term=ctsb+western+blot&report=imagesdocsum After reviewing several WB images, we observe bands of Pro CTSB at ~35 kDa and bands of mature CTSB at ~25 kDa . PMC7391525 Figure 4 *Mature form not present
- 6. Subcellular fractionation of melanoma cells revealed the presence of single chain form of CTSB on the plasma membrane. 04. HISTORY CTSB is of significant importance to cancer therapy because of its involvement with oncogenic processes in humans. 1986 CLINICAL SIGNIFICANCE
- 7. 3942 B Valley Ave Pleasanton, CA 94566 Tel: (888) 466-3604 Email: support@bosterbio.com www.bosterbio.com
Editor's Notes
- Does your next experiment involve Cathepsin B? This video is for scientists who want to run Western blot, IHC, flow or other immunoassays against CTSB.
- We will cover some basic facts, protein expression, and Cathepsin B’s expected behavior in Western blot. All information in this video is based on public information and no proprietary experimental evidence was used.
- Topic 1: Quick facts about CTSB. Cathepsin B belongs to the papain-like family of cysteine proteases. It is a bilobal protein whose catalytic site is located at the interface between the two lobes. CTSB exhibits both exopeptidase and endopeptidase activity. The precursor of CTSB is 339 amino acids long and runs about 35 kilodaltons on western blot while the mature form is 254 amino acids long and runs about 25 kilodaltons on Western blot.
- Topic 2: Where is Cathepsin B expressed? High levels of CTSB have been found in the brain, lungs, GI tract, pancreas, male and female tissues, kidney, and bladder. They are also expressed at lower levels in the heart, bone marrow, and lymphoid tissues. You can find more information on protein atlas dot org to check out which cell lines express CTSB and which ones don’t. This information will come in handy when designing positive and negative controls for western blot and immunohistochemistry.
- Topic 3: Cathepsin B in Western blotting. Antibody specificity can make or break an experiment. After looking through Western blot images from publications and antibody companies, we can see that typically two bands can be expected in a Cathepsin B Western blot, the pro-form CTSB at around 35 kilodaltons and the mature CTSB at around 25 kilodaltons. Check out the PMC link for more images, which is also provided in the description box.
- Topic 4: Some interesting facts about Cathepsin B. In 1986, subcellular fractionation of melanoma cells revealed the presence of single chain form of cathepsin B on the plasma membrane. Cathepsin B is involved in various oncogenic processes and pathologies in humans, making it of great importance in cancer therapy. The correlation of overexpression of cathepsin B with metastatic phenotypes in cancer has been shown in various studies. Abnormal regulation of CTSB causes the cell to acquire an oncogenic character. Suppressing the expression of CTSB slows down the infiltrative behaviour of tumor cells. Therefore, targeting the activity or expression of cathepsin B is of significant therapeutic importance for cancer treatment.