Does your next experiment involve Angiotensin-converting enzyme (ACE)? This is a presentation about ACE intended for scientists who are designing controls and performing immunoassays detecting ACE. It contains useful info such as Western blot band size, protein expression, and interesting facts.
Anti-Angiotensin Converting Enzyme 1/ACE Antibody (PA2196-2): https://www.bosterbio.com/anti-angiotensin-converting-enzyme-1-antibody-pa2196-2-boster.html
References: Uniprot.org, ProteinAtlas.org, PMC3322615, PMC5722543, PMC6672927, PMC3389005
Learn more about ACE (infographic and discussion): https://www.bosterbio.com/bosterbio-gene-info-cards/ACE
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3. ● Angiotensin-converting
enzyme (ACE)
● Enzyme responsible for cleaving
angiotensin I to angiotensin II
● Role in blood pressure control
● ACE is 1,306 amino acids long
● Protein size: ~150 kDa
01. FACTS
AlphaFold predicted structure
Image source: https://alphafold.ebi.ac.uk/entry/P12821
5. 03. ACE WESTERN BLOT
342 images available on PMC:
https://www.ncbi.nlm.nih.gov/pmc/?term=ACE+western+blot&report=imagesdocsum
After reviewing several WB images, we observe ACE at ~150 kDa
PMC3322615 Figure 3
PMC6672927 Figure 1
Boster Bio ACE
Antibody (PA2196-2,
Rabbit Polyclonal)
PMC5722543 Figure 2
PMC3389005 Figure 8
6. Originally called hypertension-
converting enzyme, ACE was
discovered by Leonard T. Skeggs,
Jr. in 1956.
04. HISTORY
ACE inhibitors are used to help treat
hypertension, chronic kidney
disease, and heart failure.
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Editor's Notes
Does your next experiment involve ace? This video is for scientists who want to run Western blot, IHC, flow or other immunoassays against ace.
We will cover some basic facts, protein expression, and ace’s expected behavior in Western blot. All information in this video is based on public information and no proprietary experimental evidence was used.
Topic 1: Quick facts about ace. As the name suggests, angiotensin-converting enzyme, or ace, is an enzyme responsible for cleaving angiotensin 1 into angiotensin 2. Ace plays an important role in blood pressure control. The protein is 1,306 amino acids long and runs about 150 kilodaltons on a western blot.
Topic 2: Where is Ace? Ace is highly expressed in the gastrointestinal tract, as well as the respiratory system, male and female tissues. You can find more information on protein atlas dot org to check out which cell lines express ACE and which ones don’t. This information will come in handy when designing positive and negative controls for western blot and immunohistochemistry.
Topic 3: Ace in Western blotting. Antibody specificity can make or break an experiment. After looking through Western blot images from publications and antibody companies, we can see that typically one band can be expected in an Ace western blot at about 150 kilodaltons. Check out the PMC link for more images, which is also provided in the description box.
Topic 4: Some interesting facts about Ace. Ace was originally known as hypertension-converting enzyme when it was first discovered by Leonard T Skeggs in 1956. Since then, research on Ace has led to the creation of Ace inhibitors for clinical use. Ace inhibitors have been used to help treat hypertension by decreasing blood pressure. In addition, ACE inhibitors have been used to help treat chronic kidney disease and heart failure.