Learn BMP-2 in 3 minutes | Bone morphogenetic protein 2
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Does your next experiment involve Bone morphogenetic protein 2 (BMP-2)? This is a presentation about BMP-2 intended for scientists who are designing controls and performing immunoassays detecting BMP-2. It contains useful info such as Western blot band size, protein expression, and interesting facts. Anti-BMP2 Rabbit Polyclonal Antibody (A00338): https://www.bosterbio.com/anti-bmp2-rabbit-polyclonal-antibody-a00338-boster.html References: Uniprot.org, ProteinAtlas.org, PMC5645599, PMC8082363, PMC5425130, PMC8170469 Learn more about BMP-2 (infographic and discussion): https://www.bosterbio.com/bosterbio-gene-info-cards/BMP2 Boster Biological Technology Website: www.bosterbio.com Email: support@bosterbio.com
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Learn BMP-2 in 3 minutes | Bone morphogenetic protein 2
- 1. www.bosterbio.com BMP-2 IN 3 MINUTES Quick facts, control designs, Western Blot MW. Info you can use to find the best BMP-2 antibody.
- 2. FACTS 01. Function, structure, etc. TISSUES 02. Expressing tissues/cells WESTERN BLOT 03. Expected band sizes HISTORY 04. …and medical significance TABLE OF CONTENTS
- 3. ● Bone morphogenetic protein 2 (BMP-2) ● Growth factor ● Important for development of the heart, brain, and bones ● 396 amino acids long ● Protein size: ~45 kDa 01. FACTS AlphaFold predicted structure Image source: https://alphafold.ebi.ac.uk/entry/P12643
- 4. 02. TISSUES Image source: https://www.proteinatlas.org/ENSG00000125845-BMP2/tissue
- 5. 03. BMP-2 WESTERN BLOT 735 images available on PMC: https://www.ncbi.nlm.nih.gov/pmc/?term=bmp2+western+blot&report=imagesdocsum After reviewing several WB images, we observe BMP-2 at ~45 kDa PMC5645599 Figure 3 PMC5425130 Figure 1 Boster Bio BMP-2 Antibody (A00338, Rabbit Polyclonal) PMC8170469 Figure 4 PMC8082363 Figure 3
- 6. BMP-2 was discovered by Marshall R. Urist in 1965. Since then, around 20 total BMP members have been characterized. 04. HISTORY BMP-2 plays an important role in bone development. Clinically, BMP-2 is associated with Brachydactyly type A2, a condition impacting bones in the hands and feet. 1965 CLINICAL POTENTIAL
- 7. 3942 B Valley Ave Pleasanton, CA 94566 Tel: (888) 466-3604 Email: support@bosterbio.com www.bosterbio.com
Editor's Notes
- Does your next experiment involve BMP2? This video is for scientists who want to run Western blot, IHC, flow or other immunoassays against BMP2.
- We will cover some basic facts, protein expression, and BMP2’s expected behavior in Western blot. All information in this video is based on public information and no proprietary experimental evidence was used.
- Topic 1: Quick facts about BMP2. Bone morphogenetic protein 2 or BMP2 is an important growth factor involved in the development of the heart, brain, and bones. BMP2 is 396 amino acids long and runs about 45 kilodaltons on a western blot.
- Topic 2: Where is BMP2 expressed? BMP2 RNA is expressed in multiple tissues, such as the bone marrow, kidney and urinary bladder, endocrine tissues, respiratory system, brain, gastrointestinal tract, and other tissues. You can find more information on protein atlas dot org to check out which cell lines express BMP2 and which ones don’t. This information will come in handy when designing positive and negative controls for western blot and immunohistochemistry.
- Topic 3: BMP2 in Western Blotting. Antibody specificity can make or break an experiment. After looking through Western blot images from publications and antibody companies, we can see that typically one band can be expected in a BMP2 western blot at about 45 kilodaltons. Check out the PMC link for more images, which is also provided in the description box.
- Topic 4: Some interesting facts about BMP2. BMP2 was discovered in 1965 by Marshall R. Urist. Since then, research on BMP has expanded and about 20 BMP members have been characterized. BMP2 plays an important role in bone development. Clinically, BMP2 is associated with Brachydactyly type A2, a condition that impacts bones in the hands and feet.