Learn uPAR in 3 minutes | Urokinase plasminogen activator surface receptor, PLAUR, CD87
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Does your next experiment involve Plasminogen Activator, Urokinase Receptor (PLAUR)? This is a presentation about uPAR/PLAUR intended for scientists who are designing controls and performing immunoassays detecting uPAR (Urokinase plasminogen activator surface receptor). It contains useful info such as Western blot band size, protein expression, and interesting facts Anti-UPA Receptor/Plaur Antibody Picoband™ (A00993-2): https://www.bosterbio.com/anti-upa-receptor-picoband-trade-antibody-a00993-2-boster.html References: Uniprot.org, ProteinAtlas.org, PMC2586916, PMC6653070, PMC4159539, PMC6355443 Learn more about uPAR/PLAUR (infographic and discussion): https://www.bosterbio.com/bosterbio-gene-info-cards/PLAUR Boster Biological Technology Website: www.bosterbio.com Email: support@bosterbio.com
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Learn uPAR in 3 minutes | Urokinase plasminogen activator surface receptor, PLAUR, CD87
- 1. www.bosterbio.com uPAR IN 3 MINUTES Quick facts, control designs, Western Blot MW. Info you can use to find the best PLAUR antibody.
- 2. FACTS 01. Function, structure, etc. TISSUES 02. Expressing tissues/cells WESTERN BLOT 03. Expected band sizes HISTORY 04. …and medical significance TABLE OF CONTENTS
- 3. ● Urokinase plasminogen activator surface receptor (uPAR) ● Also called CD87 or PLAUR ● Acts as a receptor for urokinase plasminogen activator ● Promotes formation of plasmin ● uPAR is 335 amino acids long ● Protein size: ~37 kDa 01. FACTS AlphaFold predicted structure Image source: https://alphafold.ebi.ac.uk/entry/Q03405
- 4. 02. TISSUES Image source: https://www.proteinatlas.org/ENSG00000011422-PLAUR/tissue
- 5. 03. uPAR WESTERN BLOT 344 images available on PMC: https://www.ncbi.nlm.nih.gov/pmc?term=uPAR+western+blot&report=imagesdocsum After reviewing several WB images, we observe PLAUR (uPAR) at ~37 kDa. PMC2586916 Figure 4 PMC4159539 Figure 2 Boster Bio PLAUR Antibody (A00993-2, Rabbit Polyclonal) PMC6653070 Figure 6 PMC6355443 Figure 3
- 6. Evidence for PLAUR was first found as a binding site for urokinase-type plasminogen activator. 04. HISTORY Numerous studies have identified elevated levels of soluble PLAUR (uPAR) is indicative of a worse prognosis in patients with cancer. 1985 CLINICAL POTENTIAL
- 7. 3942 B Valley Ave Pleasanton, CA 94566 Tel: (888) 466-3604 Email: support@bosterbio.com www.bosterbio.com
Editor's Notes
- Does your next experiment involve uPAR or Plaur? This video is for scientists who want to run Western blot, IHC, flow or other immunoassays against uPAR.
- We will cover some basic facts, protein expression, and uPAR’s expected behavior in Western blot. All information in this video is based on public information and no proprietary experimental evidence was used.
- Topic 1: Quick facts about uPAR. Urokinase plasminogen activator surface receptor, also called uPAR, Plaur, or CD87, acts as a receptor for urokinase plasminogen activator. uPAR promotes the formation of plasmin. The protein is 335 amino acids long and runs about 37 kilodaltons on a western blot.
- Topic 2: Where is uPAR expressed? uPAR is highly expressed in the bone marrow and lymphoid tissues. It is also expressed in the respiratory system, liver and gallbladder, kidney and urinary bladder. You can find more information on protein atlas dot org to check out which cell lines express Plaur and which ones don’t. This information will come in handy when designing positive and negative controls for western blot and immunohistochemistry.
- Topic 3: Plaur in Western blotting. Antibody specificity can make or break an experiment. After looking through Western blot images from publications and antibody companies, we can see that typically one band can be expected in a uPAR at about 37 kilodaltons. A highly glycosylated uPAR can be detected at around 55-65 kilodaltons. Check out the PMC link for more images, which is also provided in the description box.
- Topic 4: Some interesting facts about uPAR. Evidence for Plaur was first found in 1985, as a binding site for urokinase-type plasminogen activator. Since this discovery nearly forty years ago, numerous studies have identified elevated levels of soluble Plaur, or uPAR, is indicative of a worse prognosis in patients with cancer.