Does your next experiment involve Kidney injury molecule 1 (KIM-1)? This is a presentation about KIM1/TIM1/HAVCR1 intended for scientists who are designing controls and performing immunoassays detecting KIM-1/TIM-1/HAVCR1. It contains useful info such as Western blot band size, protein expression, and interesting facts.
Anti-TIM 1/HAVCR1 Antibody (PA1624): https://www.bosterbio.com/anti-tim-1-antibody-pa1624-boster.html
References: Uniprot.org, ProteinAtlas.org, PMC4552842, PMC8134587, PMC8139578, PMC7414978
Learn more about KIM-1/TIM-1/HAVCR1 (infographic and discussion): https://www.bosterbio.com/bosterbio-gene-info-cards/HAVCR1
Boster Biological Technology
Website: www.bosterbio.com
Email: support@bosterbio.com
5. 03. KIM-1 WESTERN BLOT
167 images available on PMC:
https://www.ncbi.nlm.nih.gov/pmc?term=KIM-1+western+blot&report=imagesdocsum
KIM-1 WB: ~35-75 kDa (more commonly ~50 kDa)
PMC7414978 Figure 1
Boster Bio Anti-TIM 1
Antibody (PA1624,
Rabbit Polyclonal)
PMC8134587 Figure 6
PMC8139578 Figure 2
PMC4552842 Figure 2
6. KIM-1 acts as a biomarker of
kidney injury and has been
proposed to be involved in kidney
repair as well.
04. SIGNIFICANCE
HAVCR1 acts as a surface
molecule for the entry of viruses
into the cell, including Ebola,
hepatitis A virus, and dengue.
KIDNEY INJURY VIRAL INFECTION
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Editor's Notes
Does your next experiment involve KIM-1? This video is for scientists who want to run Western blot, IHC, flow or other immunoassays against KIM-1.
We will cover some basic facts, protein expression, and KIM-1’s expected behavior in Western blot. All information in this video is based on public information and no proprietary experimental evidence was used.
Topic 1: Quick facts about KIM-1. Kidney injury molecule or KIM-1 is also known as HAVCR1 or TIM-1. It is a part of the TIM family of proteins. This protein is involved in the entry of many viruses into the cell, such as Ebola, hepatitis A, and dengue. KIM-1 is 344 amino acids long and runs about 40 kilodaltons on Western blot.
Topic 2: Where is KIM-1 expressed? High levels of KIM-1 have been found in the intestines, appendix, liver, gallbladder, kidneys, and bladder. It is also expressed at lower levels in the female and male reproductive tissues, skeletal muscles, and the brain. Unsurprisingly, the highest levels of KIM-1 RNA can be found in the kidneys and the intestines. You can find more information at protein atlas dot org including which cell lines express KIM-1 and which ones don’t. This information will come in handy when designing positive and negative controls for western blot and immunohistochemistry.
Topic 3: KIM-1 in Western blotting. Antibody specificity can make or break an experiment. After looking through Western blot images from publications and antibody companies, we can see that the protein typically appears around 50 kilodaltons in a Western blot. However, KIM-1 has been observed at multiple different sizes ranging from around 35 kilodaltons to 75 kilodaltons. Check out the PMC link for more images, which is also provided in the description box.
Topic 4: Some interesting facts about KIM-1. As the full name suggests, KIM-1 is known to be involved in kidney injury as a biomarker of the damage. However, recent reports suggest that it may also be involved in kidney repair. This molecule is also known as HAVCR1, or hepatitis A virus cellular receptor 1. HAVCR1 is involved in the entry of viruses into our cells. Viruses such as Ebola, dengue, and hepatitis A virus bind to KIM-1 and gain entry into the cells. Many cell lines that were poorly susceptible against these viruses have demonstrated enhanced susceptibility after increasing KIM-1 expression. Conversely, knocking down KIM-1 reduces the viruses’ infectivity in susceptible cell lines. This makes KIM-1 a particularly interesting molecule to study as a potential therapeutic or preventive target for future outbreaks of deadly viruses.