Learn CXCL13 in 3 minutes | Chemokine (C-X-C motif) ligand 13
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Does your next experiment involve Chemokine (C-X-C motif) ligand 13 (CXCL13)? This is a presentation about CXCL13/BCA1/BLC intended for scientists who are designing controls and performing immunoassays detecting CXCL13/BCA1/BLC. It contains useful info such as Western blot band size, protein expression, and interesting facts. Anti-BCA1/CXCL13 Antibody Picoband™ (PB9999): https://www.bosterbio.com/anti-bca1-picoband-trade-antibody-pb9999-boster.html References: Uniprot.org, ProteinAtlas.org, GeneCards, PMC5307320, PMC7422843, PMC4940825 Learn more about CXCL13 (infographic and discussion): https://www.bosterbio.com/bosterbio-gene-info-cards/CXCL13 Boster Biological Technology Website: www.bosterbio.com Email: support@bosterbio.com
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Learn CXCL13 in 3 minutes | Chemokine (C-X-C motif) ligand 13
- 1. www.bosterbio.com CXCL13 IN 3 MINUTES Quick facts, control designs, Western Blot MW. Info you can use to find the best CXCL13 antibody.
- 2. FACTS 01. Function, structure, etc. TISSUES 02. Expressing tissues/cells WESTERN BLOT 03. Expected band sizes HISTORY 04. …and medical significance TABLE OF CONTENTS
- 3. ● Chemokine (C-X-C motif) ligand 13 (CXCL13) ○ B lymphocyte chemoattractant (BLC) ○ B cell-attracting chemokine 1 (BCA- 1) ● Chemotactic cytokine for B cells ● Binds to BLR1/CXCR5 ● Stimulates calcium influx into and chemotaxis of BLR1-transfected cells ● Mature form: ~12.6 kDa, 109 AAs 01. FACTS AlphaFold predicted structure Image source: https://alphafold.ebi.ac.uk/entry/F7F7W7
- 4. 02. TISSUES Image source: https://www.proteinatlas.org/ENSG00000156234-CXCL13/tissue
- 5. 03. CXCL13 WESTERN BLOT 71 images available on PMC: https://www.ncbi.nlm.nih.gov/pmc?term=CXCL13+western+blot&report=imagesdocsum After reviewing several WB images, we observe mature CXCL13 between 12-15 kDa. PMC5307320 Figure 8d PMC7422843 Figure 4e Novusbio MAB801 PMC4940825 Figure 2b Boster Bio CXCL13 Antibody (PB9999, Rabbit Polyclonal)
- 6. Initially detected in stromal cells of B cell follicles and associated with the recruitment of B cells and T cells subsets in 1996 04. HISTORY Interest in using CXCL13 as a prognostic and predictive factor in some ICI-responsive cancer tumor types 1982 CLINICAL POTENTIAL
- 7. 3942 B Valley Ave Pleasanton, CA 94566 Tel: (888) 466-3604 Email: support@bosterbio.com www.bosterbio.com
Editor's Notes
- Does your next experiment involve CXCL13? This video is for scientists who want to run Western blot, IHC, flow or other immunoassays against CXCL13.
- We will cover some basic facts, protein expression, and CXCL13’s expected behavior in Western blot. All information in this video is based on public information and no proprietary experimental evidence was used.
- Topic 1: Quick facts about CXCL13. CXCL13, also known as BLC or BCA1, is a chemotactic cytokine for B lymphocytes that binds to BLR1 or CXCR5. It stimulates calcium influx into, and chemotaxis of, cells transfected with Burkitt's lymphoma receptor 1. The mature form of CXCL13 is 109 amino acids long and runs about 12.6 kilodaltons on Western blot.
- Topic 2: Where is CXCL13 expressed? High levels of CXCL13 RNA expression have been found in the tonsils and spleen, with the highest levels detected in the lymph node. They are also expressed at lower levels in the appendix, small intestine, stomach, thymus, prostate, urinary bladder, and liver. You can find more information about tissue expression on protein atlas dot org as well as which cell lines express CXCL13. This information will come in handy when designing positive and negative controls for western blot and immunohistochemistry.
- Topic 3: CXCL13 in Western blotting. Antibody specificity can make or break an experiment. After looking through Western blot images from publications and antibody companies, we can see that typically one band can be expected in a CXCL13 Western blot with the mature form of CXCL13 between 12 and 15 kilodaltons. Check out the PMC link for more images, which are also provided in the description box.
- Topic 4: Some interesting facts about CXCL13. Overall, the CXCL13/CXCR5 signaling axis can recruit immune cells and enhance the capacity to defend the body. It was initially detected in stromal cells of B cell follicles and associated with the recruitment of B cells and T cells subsets in 1996. Therefore, the scientific community is interested in the use of CXCL13 as a prognostic and predictive factor in some ICI responsive cancer tumor types.