Learn CD133 in 3 minutes | Prominin-1, PROM1
•Download as PPTX, PDF•
0 likes•70 views
Does your next experiment involve CD133 (Prominin-1)? This is a presentation about CD133/Prominin-1/PROM1 intended for scientists who are designing controls and performing immunoassays detecting Prominin-1. It contains useful info such as Western blot band size, protein expression, and interesting facts. Anti-PROM1 Antibody Picoband™ (PB9156): https://www.bosterbio.com/anti-prom1-picoband-trade-antibody-pb9156-boster.html References: Uniprot.org, ProteinAtlas.org, PMC6763460, PMC2713505, PMC6030467 Learn more about CD133/Prominin-1/PROM1 (infographic and discussion): https://www.bosterbio.com/bosterbio-gene-info-cards/PROM1 Boster Biological Technology Website: www.bosterbio.com Email: support@bosterbio.com
Recommended
Recommended
More Related Content
Similar to Learn CD133 in 3 minutes | Prominin-1, PROM1
Similar to Learn CD133 in 3 minutes | Prominin-1, PROM1 (20)
More from Boster Biological Technology
More from Boster Biological Technology (8)
Recently uploaded
Recently uploaded (20)
Learn CD133 in 3 minutes | Prominin-1, PROM1
- 1. www.bosterbio.com CD133 IN 3 MINUTES Quick facts, control designs, Western Blot MW. Info you can use to find the best CD133 antibody.
- 2. FACTS 01. Function, structure, etc. TISSUES 02. Expressing tissues/cells WESTERN BLOT 03. Expected band sizes SIGNIFICANCE 04. Clinical potential TABLE OF CONTENTS
- 3. ● CD133 ○ also known as Prominin-1 ● Membrane glycoprotein ● Stem cell biomarker ● Cleaved into: ○ 19-amino-acid long signal peptide ○ mature CD133 (846 amino acids long) ● Western blot: ~97-130 kDa 01. FACTS AlphaFold predicted structure Image source: https://alphafold.ebi.ac.uk/entry/O43490
- 4. 02. TISSUES Image source: https://www.proteinatlas.org/ENSG00000007062-PROM1/tissue
- 5. 03. CD133 WESTERN BLOT 1219 images available on PMC: https://www.ncbi.nlm.nih.gov/pmc/?term=cd133+western+blot&report=imagesdocsum After reviewing several WB images, we observe CD133 at ~97-130 kDa. Boster Bio CD133 Antibody (PB9156, Rabbit Polyclonal) PMC2713505 Figure 3 PMC6763460 Figure 1 PMC6030467 Figure 1
- 6. CD133 is a stem cell biomarker. It is also found in cancer stem cells (CSCs) or tumor-initiating cells. 04. SIGNIFICANCE CD133 is a membrane glycoprotein used to separate stem cells from a mixture of cells in a tissue. STEM CELL BIOMARKER STEM CELL ISOLATION
- 7. 3942 B Valley Ave Pleasanton, CA 94566 Tel: (888) 466-3604 Email: support@bosterbio.com www.bosterbio.com
Editor's Notes
- Does your next experiment involve CD133? This video is for scientists who want to run Western blot, IHC, flow or other immunoassays against CD133.
- We will cover some basic facts, protein expression, and CD133’s expected behavior in Western blot. All information in this video is based on public information and no proprietary experimental evidence was used.
- Topic 1: Quick facts about CD133. Also known as Prominin-1, CD133 is part of the prominin family of proteins. It is a membrane glycoprotein that serves as a biomarker for stem cells. The protein consists of a 19-amino-acid long signal peptide that is cleaved off to generate the mature CD133 which is 846 amino acids long. CD133 runs about 97 to 130 kilodaltons on Western blot.
- Topic 2: Where is CD133 expressed? High levels of CD133 have been found in the respiratory system, the renal system, female reproductive organs, and the hepatic tissues. High RNA expression has been reported for the retina, but there is limited protein expression. CD133 is also expressed at lower levels in the endocrine tissues, male reproductive tissues, skin, bone marrow, pancreas, and muscles. You can find more information at protein atlas dot org including which cell lines express CD133 and which ones don’t. This information will come in handy when designing positive and negative controls for western blot and immunohistochemistry.
- Topic 3: CD133 in Western blotting. Antibody specificity can make or break an experiment. After looking through Western blot images from publications and antibody companies, we can see that typically a band ranging from 97 to 130 kilodaltons can be expected in a CD133 Western blot. Check out the PMC link for more images, which is also provided in the description box.
- Topic 4: Some interesting facts about CD133. Prominin-1 is a membrane glycoprotein that is used to identify stem cells or stem-like cells. It has been extensively used to isolate stem cells from pathological tissue mixtures. Furthermore, scientists are using CD133 to identify and target cancer stem cells. This biomarker holds significant therapeutic potential, which has been demonstrated in recent studies involving metastatic melanomas. In the future, we may see more research development related to cancer stem cell targeting using CD133.