Does your next experiment involve Interleukin 6? This is a presentation about IL-6 intended for scientists who are designing controls and performing immunoassays detecting Interleukin-6. It contains useful info such as Western blot band size, protein expression, and interesting facts.
Anti-Interleukin-6 Antibody (PA1700):
https://www.bosterbio.com/anti-il-6-antibody-pa1700-boster.html
References: Uniprot.org, ProteinAtlas.org, GeneCards, PMC3580035, PMC4107025, PMC5522095, PMC5817102
Learn more about IL-6 (infographic and discussion): https://www.bosterbio.com/bosterbio-gene-info-cards/IL6
Boster Biological Technology
Website: www.bosterbio.com
Email: support@bosterbio.com
5. 8087 images available on PMC:
https://www.ncbi.nlm.nih.gov/pmc/?term=il-6+western+blot&report=imagesdocsum
After reviewing several WB images, we observe one IL-6 band at ~ 24 kDa.
03. IL-6 WESTERN BLOT
PMC3580035 Figure 2 PMC4107025 Figure 3
R&D Systems
AF506
Boster Bio IL-6
Antibody (PA1700,
Rabbit Polyclonal)
PMC5817102
Figure 4
PMC5522095 Figure 6
6. IL-6 was originally identified as a B
cell stimulatory factor initiating IgG
production.
04. HISTORY
Anti-IL6 therapy is a new strategy
in the inflammatory autoimmune
diseases and cancer.
1986 CLINICAL POTENTIAL
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Editor's Notes
Does your next experiment involve Interleukin 6? This video is for scientists who want to run Western blot, IHC, flow or other immunoassays against IL6.
We will cover some basic facts, protein expression, and interleukin 6’s expected behavior in Western blot. All information in this video is based on public information and no proprietary experimental evidence was used.
Topic 1: Quick facts about IL6. Interleukin-6 is a cytokine with a wide variety of biological functions in immunity, hematopoiesis, tissue regeneration, and metabolism. IL6 is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through IL6 receptor alpha. Rapid production of interleukin 6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. Interleukin 6 is 212 amino acids long and runs about 24 kilodaltons on Western blot.
Topic 2: Where is interleukin 6 expressed? High RNA expression levels of IL6 is found in the brain, lungs, adipose tissue, bone marrow, and skeletal muscle cells, with the highest levels detected in the urinary bladder. They are also expressed at lower levels in the kidney, liver, thyroid gland, pancreas, skin, prostate and retina. Elevated levels of the encoded protein have been found in virus infections, including COVID-19. You can find more information on protein atlas dot org to check out which cell lines express interleukin 6 and which ones don’t. This information will come in handy when designing positive and negative controls for western blot and immunohistochemistry.
Topic 3: Interleukin 6 in Western blotting. Antibody specificity can make or break an experiment. After looking through Western blot images from publications and antibody companies, we can see that typically one band at around 24 kilodaltons can be expected in an IL6 Western blot. Check out the PMC link for more images, which is also provided in the description box.
Topic 4: Some interesting facts about IL6. Interleukin 6 was first discovered in 1986 as a T cell-derived lymphokine that induces the final maturation of B cells into antibody-producing cells. IL6 can simultaneously elicit distinct or even contradictory physiopathological processes. When taking into account all the accumulated basic research on the various aspects of this molecule, therapeutic strategies to inhibit IL6 signaling have proven effective in treating experimental models of autoimmune and chronic inflammatory diseases.