Learn IGF-1 in 3 minutes | Insulin-like growth factor I
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Does your next experiment involve Insulin-like growth factor I (IGF-I, IGF-1)? This is a presentation about IGF1 intended for scientists who are designing controls and performing immunoassays detecting IGF1. It contains useful info such as Western blot band size, protein expression, and interesting facts. Anti-IGF1 Mouse Monoclonal Antibody (M00148-1): https://www.bosterbio.com/anti-igf1-mouse-monoclonal-antibody-clone-id-oti4b12-m00148-1-boster.html References: Uniprot.org, ProteinAtlas.org, PMC5867890, PMC7450330, PMC7262660, PMC7401641 Learn more about IGF1 (infographic and discussion): https://www.bosterbio.com/bosterbio-gene-info-cards/IGF1 Boster Biological Technology Website: www.bosterbio.com Email: support@bosterbio.com
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Learn IGF-1 in 3 minutes | Insulin-like growth factor I
- 1. www.bosterbio.com IGF-1 IN 3 MINUTES Quick facts, control designs, Western Blot MW. Info you can use to find the best IGF1 antibody.
- 2. FACTS 01. Function, structure, etc. TISSUES 02. Expressing tissues/cells WESTERN BLOT 03. Expected band sizes HISTORY 04. …and medical significance TABLE OF CONTENTS
- 3. ● Insulin-like growth factor I (IGF- I, IGF-1) ● Plays an important role in growth and development ● Binds to IGF1-Receptor to initiate signaling pathways ● 195 amino acids long ● Protein size: ~22 kDa 01. FACTS AlphaFold predicted structure Image source: https://alphafold.ebi.ac.uk/entry/P05019
- 4. 02. TISSUES Image source: https://www.proteinatlas.org/ENSG00000017427-IGF1/tissue
- 5. 03. IGF-1 WESTERN BLOT 412 images available on PMC: https://www.ncbi.nlm.nih.gov/pmc?term=IGF1+western+blot&report=imagesdocsum After reviewing several WB images, we observe IGF1 at ~22 kDa PMC5867890 Figure 3 PMC7450330 Figure 6 Boster Bio IGF1 Antibody (M00148-1, Mouse Monoclonal) PMC7262660 Figure 3 PMC7401641 Figure 3
- 6. IGF-1 was discovered by Salmon and Daughaday in 1957, but the protein was not termed IGF-1 until 1976. 04. HISTORY The IGF-1 signaling pathway has been associated with several types of cancer and resistance to radiotherapy. 1957 CLINICAL POTENTIAL
- 7. 3942 B Valley Ave Pleasanton, CA 94566 Tel: (888) 466-3604 Email: support@bosterbio.com www.bosterbio.com
Editor's Notes
- Does your next experiment involve IGF1? This video is for scientists who want to run Western blot, IHC, flow or other immunoassays against IGF1.
- We will cover some basic facts, protein expression, and IGF1’s expected behavior in Western blot. All information in this video is based on public information and no proprietary experimental evidence was used.
- Topic 1: Quick facts about IGF1. Insulin-like growth factor 1, or IGF1, plays an important role in growth and development. IGF1 binds to the IGF1 receptor to initiate signaling pathways. The protein is 195 amino acids long and runs about 22 kilodaltons on a western blot.
- Topic 2: Where is IGF1 expressed? IGF1 RNA is expressed mostly in the liver, but it is also expressed in the bone marrow and lymphoid tissues, the male and female tissues, muscle, and connective and soft tissues. You can find more information on protein atlas dot org to check out which cell lines express IGF1 and which ones don’t. This information will come in handy when designing positive and negative controls for western blot and immunohistochemistry.
- Topic 3: IGF1 in Western blotting. Antibody specificity can make or break an experiment. After looking through Western blot images from publications and antibody companies, we can see that typically one band can be expected in a IGF1 western blot at about 22 kilodaltons. Check out the PMC link for more images, which is also provided in the description box.
- Topic 4: Some interesting facts about IGF1. IGF1 was discovered by Salmon and Daughaday in 1957, though the protein was not termed IGF1 until 1976. The IGF1 signaling pathway has been linked with several types of cancer and has been associated with resistance to radiotherapy treatment.