IFN-γ Quick Reference Guide
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Does your next experiment involve Interferon gamma? This is a presentation about IFN Gamma/IFNG intended for scientists who are designing controls and performing immunoassays detecting IFN Gamma/IFNG. It contains useful info such as Western blot band size, protein expression, and interesting facts. Anti-Interferon Gamma IFNG Rabbit Monoclonal Antibody (M00393-1): https://www.bosterbio.com/anti-interferon-gamma-rabbit-monoclonal-antibody-m00393-1-boster.html Anti-IFN Gamma Antibody (RP1001): https://www.bosterbio.com/anti-rat-ifn-gamma-antibody-rp1001-boster.html References: Uniprot.org, ProteinAtlas.org, PMID15220936, PMID9524237, PMID24253448, PMC7086207 Learn more about IFN Gamma/IFNG (infographic and discussion): https://www.bosterbio.com/bosterbio-gene-info-cards/IFNG Boster Biological Technology Website: www.bosterbio.com Email: support@bosterbio.com
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IFN-γ Quick Reference Guide
- 1. www.bosterbio.com IFN-γ IN 3 MINUTES Quick facts, control designs, Western Blot MW. Info you can use to find the best IFN-γ antibody.
- 2. FACTS 01. Function, structure, etc. TISSUES 02. Expressing tissues/cells WESTERN BLOT 03. Expected band sizes HISTORY 04. …and medical significance TABLE OF CONTENTS
- 3. ● Interferon Gamma (IFN-γ) ● Cytokine family ● Produced by NK cells and Th1 cells ● Biologically active form is a dimer ● Precursor/pro-form ○ 166 amino acids long ○ Gets cleaved into mature IFN-γ for activation ● Mature form ○ 138 amino acids long ○ ~23 kDa 01. FACTS AlphaFold predicted structure Image source: https://alphafold.ebi.ac.uk/entry/P01579
- 4. 02. TISSUES Image source: https://www.proteinatlas.org/ENSG00000111537-IFNG/tissue
- 5. 03. IFN-γ WESTERN BLOT 3252 images available on PMC: https://www.ncbi.nlm.nih.gov/pmc/?term=ifn- gamma+western+blot&report=imagesdocsum After reviewing several WB images, we observe mature IFN-γ at ~17-25 kDa. PMID24253448 Figure 1 Boster Anti-IFN-γ Rabbit Monoclonal Antibody (M00393-1) PMID15220936 Figure 5 PMC7086207 Figure 3 Boster Anti-IFN Gamma Antibody (RP1001) PMID9524237 Figure 6
- 6. Promotes antiproliferative activity against cancer 04. HISTORY Helps remodel the arteries in the uterus to allow the growth of placenta ANTI-CANCER PREGNANCY INITIATION
- 7. 3942 B Valley Ave Pleasanton, CA 94566 Tel: (888) 466-3604 Email: support@bosterbio.com www.bosterbio.com
Editor's Notes
- Does your next experiment involve IFN gamma? This video is for scientists who want to run Western blot, IHC, flow or other immunoassays against IFN gamma.
- We will cover some basic facts, protein expression, and IFN gamma’s expected behavior in Western blot. All information in this video is based on public information and no proprietary experimental evidence was used.
- Topic 1: Quick facts about IFN gamma. Interferon gamma is part of the cytokine family of secreted proteins produced by immune cells such as natural killer cells and T helper type 1 cells. It is heavily involved in the activation of antimicrobial, antiviral, and antitumor immune responses. While IFN gamma has some structural differences across vertebrates, its structure and function remain largely conserved. The precursor of IFN gamma is 166 amino acids long while the mature form is 138 amino acids long and runs about 23 kilodaltons on Western blot.
- Topic 2: Where is IFN gamma expressed? High RNA expression levels of IFN gamma have been found in the organs involved in immune cell production or immune response. It is expressed at a high level in the appendix and lymph nodes. However, the highest levels of IFN gamma are found in the bone marrow. Lungs, liver, and urinary bladder also express a considerable amount of IFN gamma. You can find more information at protein atlas dot org including which cell lines express IFN gamma and which ones don’t. This information will come in handy when designing positive and negative controls for western blot and immunohistochemistry.
- Topic 3: IFN gamma in Western blotting. Antibody specificity can make or break an experiment. After looking through Western blot images from publications and antibody companies, we can see that typically one band can be expected in an IFN gamma Western blot from around 17 to 25 kilodaltons as a result of differential glycosylation. Check out the PMC link for more images, which is also provided in the description box.
- Topic 4: Some interesting facts about Interferon gamma. IFN gamma has been shown to promote anti-cancer activity in vitro. This usually happens through the induction of apoptosis, but autophagy has been reported as another observed mechanism. Interestingly, IFN gamma is also involved with the thinning of decidual arterial walls to enhance blood flow to the embryo implantation site, which helps with placental development.