Learn LCN2/NGAL in 3 minutes | Lipocalin-2
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This document provides key information about the protein Lipocalin-2 (LCN2), also known as neutrophil gelatinase-associated lipocalin (NGAL). It discusses LCN2's function, structure, expressing tissues/cells, expected band size on a Western Blot, role in the innate immune response, and clinical significance in acute kidney injury. The document includes diagrams of LCN2's predicted structure and expressing tissues. It also references multiple published Western Blots demonstrating that LCN2 appears at around 25 kDa.
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Learn LCN2/NGAL in 3 minutes | Lipocalin-2
- 1. www.bosterbio.com LCN2 IN 3 MINUTES Quick facts, control designs, Western Blot MW. Info you can use to find the best LCN2 antibody.
- 2. FACTS 01. Function, structure, etc. TISSUES 02. Expressing tissues/cells WESTERN BLOT 03. Expected band sizes HISTORY 04. …and medical significance TABLE OF CONTENTS
- 3. ● Lipocalin-2 (LCN2) ○ aka neutrophil gelatinase-associated lipocalin (NGAL) ● 198 amino acids long ● Size: ~25 kDa ● Binds small, lipophilic molecules ● Limited if any homology conserved amongst more mammals 01. FACTS AlphaFold predicted structure Image source: https://alphafold.ebi.ac.uk/entry/P80188
- 4. 02. TISSUES Image source: https://www.proteinatlas.org/ENSG00000148346-LCN2/tissue
- 5. 03. LCN2 WESTERN BLOT 243 images available on PMC: https://www.ncbi.nlm.nih.gov/pmc/?term=lcn2+western+blot&report=imagesdocsum After reviewing several WB images, we observe LCN2 roughly at ~25 kDa. PMC2964970 Figure 1 PMC5461642 Figure 5 PMC6601774 Figure 7 PMC6601774 Figure 1 Boster Bio LCN2 Antibody (PB9609, Rabbit Polyclonal) PMC4511361 Figure 2
- 6. LCN2 mediates the innate immune response to pathogens by iron sequestration, which inhibits pathogen growth. 04. HISTORY In acute kidney injury, LCN2 is secreted in high levels in the blood and urine within 2 hours of injury. 1989 CLINICAL POTENTIAL
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Editor's Notes
- Does your next experiment involve Lipocalin-2? This video is for scientists who want to run Western blot, IHC, flow or other immunoassays against LCN2.
- We will cover some basic facts, protein expression, and Lipocalin-2’s expected behavior in Western blot. All information in this video is based on public information and no proprietary experimental evidence was used.
- Topic 1: Quick facts about LCN2. Lipocalin-2, also referred to as neutrophil gelatinase-associated lipocalin or NGAL, belongs to the Calycin superfamily. It binds small lipophilic molecules, is involved with the innate immune system, and acts as a biomarker for the kidney. LCN2 has limited, if any, conserved homology amongst most mammals. It is 198 amino acids long and runs about 25 kilodaltons on Western blot.
- Topic 2: Where is Lipocalin-2 expressed? High levels of LCN2 have been found in the bone marrow, cervix, and kidneys. They are also expressed at lower levels in the nasopharynx, bronchus, salivary glands, stomach, colon, rectum, spleen, and skin. It is released into and can be detected in blood, saliva and urine. You can find more information at protein atlas dot org including which cell lines express LCN2 and which ones don’t. This information will come in handy when designing positive and negative controls for western blot and immunohistochemistry.
- Topic 3: LCN2 in Western blotting. Antibody specificity can make or break an experiment. After looking through Western blot images from publications and antibody companies, we can see that typically one band can be expected in a LCN2 Western blot at around 25 kilodaltons. Check out the PMC link for more images, which is also provided in the description box.
- Topic 4: Some interesting facts about LCN2. Discovered in 1989 in granules of neutrophils, lipocalin-2 functions as an inflammatory mediator and influences the innate immune system. It directs the immune response to pathogens by iron sequestration, which inhibits pathogen growth. LCN2 is also seen in elevated levels during acute kidney injury, allowing it to be used in early diagnosis for procedures such as chronic kidney disease, contrast-induced nephropathy, and kidney transplant.