Learn POSTN in 3 minutes | Periostin, OSF2
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Does your next experiment involve Periostin (POSTN)? This is a presentation about POSTN intended for scientists who are designing controls and performing immunoassays detecting POSTN. It contains useful info such as Western blot band size, protein expression, and interesting facts. Anti-Periostin/Postn Antibody Picoband™ (A01378): https://www.bosterbio.com/anti-periostin-picoband-trade-antibody-a01378-boster.html References: Uniprot.org, ProteinAtlas.org, PMC4826188, PMC4746667, PMC4262539, PMC3443161 Learn more about POSTN (infographic and discussion): https://www.bosterbio.com/bosterbio-gene-info-cards/POSTN Boster Biological Technology Website: www.bosterbio.com Email: support@bosterbio.com
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Learn POSTN in 3 minutes | Periostin, OSF2
- 1. www.bosterbio.com POSTN IN 3 MINUTES Quick facts, control designs, Western Blot MW. Info you can use to find the best POSTN antibody.
- 2. FACTS 01. Function, structure, etc. TISSUES 02. Expressing tissues/cells WESTERN BLOT 03. Expected band sizes HISTORY 04. …and medical significance TABLE OF CONTENTS
- 3. ● Periostin (POSTN, OSF2) ● Extracellular matrix protein involved in wound healing ● 836 amino acids long ● Protein size: ~93 kDa 01. FACTS AlphaFold predicted structure Image source: https://alphafold.ebi.ac.uk/entry/Q15063
- 4. 02. TISSUES Image source: https://www.proteinatlas.org/ENSG00000133110-POSTN/tissue
- 5. 03. POSTN WESTERN BLOT 137 images available on PMC: https://www.ncbi.nlm.nih.gov/pmc?term=POSTN+western+blot&report=imagesdocsum After reviewing several WB images, we observe POSTN at ~93 kDa PMC4826188 Figure 4 PMC3443161 Figure 3 Boster Bio POSTN Antibody (A01378, Rabbit Polyclonal) PMC4746667 Figure 2 PMC4262539 Figure 2
- 6. In 1993, POSTN was first determined to be a protein involved in cell adhesion. 04. HISTORY POSTN has been found to be expressed highly in certain cancers, but its role in cancer is still under investigation. 1993 CLINICAL POTENTIAL
- 7. 3942 B Valley Ave Pleasanton, CA 94566 Tel: (888) 466-3604 Email: support@bosterbio.com www.bosterbio.com
Editor's Notes
- Does your next experiment involve Periostin? This video is for scientists who want to run Western blot, IHC, flow or other immunoassays against Periostin.
- We will cover some basic facts, protein expression, and Periostin’s expected behavior in Western blot. All information in this video is based on public information and no proprietary experimental evidence was used.
- Topic 1: Quick facts about Periostin. Periostin is an extracellular matrix protein that is involved in wound healing. The protein is 836 amino acids long and runs about 93 kilodaltons on a western blot.
- Topic 2: Where is Periostin? Periostin is expressed in the skin, gastrointestinal tract, liver and gallbladder, kidney, urinary tracts and other tissues. It is also expressed on the periosteum, which is a type of membrane on bones. You can find more information on protein atlas dot org to check out which cell lines express Periostin and which ones don’t. This information will come in handy when designing positive and negative controls for western blot and immunohistochemistry.
- Topic 3: Periostin in Western blotting. Antibody specificity can make or break an experiment. After looking through western blot images from publications and antibody companies, we can see that typically one band can be expected in a Periostin western blot at about 93 kilodaltons. Check out the PMC link for more images, which is also provided in the description box.
- Topic 4: Some interesting facts about Periostin. Periostin was first found to be a protein involved in cell adhesion about thirty years ago. Since then, it has been shown to overexpress in certain cancers, such as ovarian, breast, colorectal cancer, and other types of cancer. Periostin’s specific role in cancer is still under investigation.