Learn NGF/NGF Beta in 3 minutes | Beta-nerve growth factor
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Does your next experiment involve NGFβ? This is a presentation about NGF/NGF Beta intended for scientists who are designing controls and performing immunoassays detecting NGF/beta-NGF. It contains useful info such as Western blot band size, protein expression, and interesting facts. Anti-NGF/NGF Beta Antibody Picoband™ (A00341): https://www.bosterbio.com/anti-ngf-ngf-beta-picoband-trade-antibody-a00341-boster.html References: Uniprot.org, ProteinAtlas.org, GeneCards, PMC4760111, PMC6037903, PMC2650228, PMC1894684 Learn more about NGF/NGF Beta (infographic and discussion): https://www.bosterbio.com/bosterbio-gene-info-cards/NGF Boster Biological Technology Website: www.bosterbio.com Email: support@bosterbio.com
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Learn NGF/NGF Beta in 3 minutes | Beta-nerve growth factor
- 1. www.bosterbio.com NGFβ IN 3 MINUTES Quick facts, control designs, Western Blot MW. Info you can use to find the best NGFβ antibody.
- 2. FACTS 01. Function, structure, etc. TISSUES 02. Expressing tissues/cells WESTERN BLOT 03. Expected band sizes HISTORY 04. …and medical significance TABLE OF CONTENTS
- 3. ● Beta-nerve growth factor (NGF, NGF Beta, NGFβ) ● Important for the development and maintenance of the sympathetic and sensory nervous systems ● Homodimer protein ● Mature NGFβ ○ 241 amino acids long ○ MW: 26.959 kDa ● proNGF: ~130 kDa 01. FACTS AlphaFold predicted structure Image source: https://alphafold.ebi.ac.uk/entry/P01138
- 4. 02. TISSUES Image source: https://www.proteinatlas.org/ENSG00000134259-NGF/tissue
- 5. 1230 images available on PMC: https://www.ncbi.nlm.nih.gov/pmc/?term=ngf+western+blot&report=imagesdocsum After reviewing several WB images, we observe mature NGF Beta monomer at ~13 kDa. 03. NGFβ WESTERN BLOT PMC4760111 Figure 2 Boster Anti-NGF Antibody (A00341) PMC2650228 Figure 2 PMC1894684 Figure 1 PMC6037903 Figure 1
- 6. NGFβ is a neurotrophic factor and a neuropeptide that regulates the growth, maintenance, proliferation, and survival of specific neurons. 04. HISTORY Mutations in NGF have been linked with neuropathy, particularly hereditary sensory and autonomic neuropathy (HSAN). 1950s CLINICAL POTENTIAL
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Editor's Notes
- Does your next experiment involve NGF Beta? This video is for scientists who want to run Western blot, IHC, flow or other immunoassays against NGF Beta.
- We will cover some basic facts, protein expression, and NGF Beta’s expected behavior in Western blot. All information in this video is based on public information and no proprietary experimental evidence was used.
- Topic 1: Quick facts about NGF. Beta-nerve growth factor or NGF Beta is important for the development and maintenance of the sympathetic and sensory nervous systems. Unlike the mature form, the immature NGF precursor, or proNGF, functions as a ligand for the heterodimeric receptor and promotes neuronal apoptosis. The precursor NGF is an approximately 130 kilodaltons complex while the mature form is 241 amino acids long with a molecular weight of about 27 kilodaltons.
- Topic 2: Where is NGF Beta expressed? High levels of NGF beta RNA have been found in ovaries and heart muscles. They are also expressed in fibroblasts involved with the extracellular matrix organization. You can find more information on protein atlas dot org to check out which cell lines express NGF beta and which ones don’t. This information will come in handy when designing positive and negative controls for western blot and immunohistochemistry.
- Topic 3: NGF Beta in Western blotting. Antibody specificity can make or break an experiment. After looking through Western blot images from publications and antibody companies, we can see that typically one band can be expected in an NGF Beta Western blot at around 13 kilodaltons, which is the expected molecular weight of mature NGF monomer. Check out the PMC link for more images, which is also provided in the description box.
- Topic 4: Some interesting facts about NGF. NGF Beta is a neurotrophic factor and a neuropeptide involved in regulating the growth, maintenance, proliferation, and survival of specific neurons. It was first discovered in the 1950s, but was widely recognized in 1986. Mutations in NGF have been associated with neuropathy, particularly hereditary sensory and autonomic neuropathy. NGF is also a prognostic marker in renal, head, and neck cancers.