Learn NEFH/NF-H in 3 minutes | Neurofilament Heavy Chain
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Does your next experiment involve Neurofilament Heavy Polypeptide (NEFH)? This is a presentation about NEFH/NF-H intended for scientists who are designing controls and performing immunoassays detecting NEFH/NF-H. It contains useful info such as Western blot band size, protein expression, and interesting facts. Anti-NEFH/Nf H Rabbit Monoclonal Antibody (M05307-2): https://www.bosterbio.com/anti-nefh-rabbit-monoclonal-antibody-m05307-2-boster.html References: Uniprot.org, ProteinAtlas.org, PMC6834541, PMC6054723, PMC4285109 Learn more about NEFH/NF-H (infographic and discussion): https://www.bosterbio.com/bosterbio-gene-info-cards/NEFH Boster Biological Technology Website: www.bosterbio.com Email: support@bosterbio.com
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Learn NEFH/NF-H in 3 minutes | Neurofilament Heavy Chain
- 1. www.bosterbio.com NEFH IN 3 MINUTES Quick facts, control designs, Western Blot MW. Info you can use to find the best NEFH antibody.
- 2. FACTS 01. Function, structure, etc. TISSUES 02. Expressing tissues/cells WESTERN BLOT 03. Expected band sizes SIGNIFICANCE 04. Clinical potential TABLE OF CONTENTS
- 3. ● Neurofilament heavy polypeptide (NEFH) ● Component of neuronal cytoskeleton neurofilaments (NEFs) ● NEFH works together with the neurofilament light (NEFL) and neurofilament medium (NEFM) chains ● 1,026 amino acids long ● Size: ~200 kDa 01. FACTS AlphaFold predicted structure Image source: https://alphafold.ebi.ac.uk/entry/P12036
- 4. 02. TISSUES Image source: https://www.proteinatlas.org/ENSG00000100285-NEFH/tissue
- 5. 03. NEFH WESTERN BLOT 234 images available on PMC: https://www.ncbi.nlm.nih.gov/pmc/?term=nf+h+western+blot&report=imagesdocsum After reviewing several WB images, we observe NEFH at ~200 kDa. PMC6834541 Figure 8 PMC4285109 Figure 4 PMC6054723 Figure 6 Boster Bio NEFH Antibody (M05307-2, Rabbit Monoclonal)
- 6. Along with NEFL and NEFM, NEFH forms the neurofilaments in the brain and spinal cord. 04. SIGNIFICANCE NEFH is a biomarker for neuronal damage. NEFH gene mutations are also associated with amyotrophic lateral sclerosis (ALS). NEUROFILAMENT FORMATION NEURONAL DAMAGE
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Editor's Notes
- Does your next experiment involve NEFH? This video is for scientists who want to run Western blot, IHC, flow or other immunoassays against NEFH.
- We will cover some basic facts, protein expression, and NEFH’s expected behavior in Western blot. All information in this video is based on public information and no proprietary experimental evidence was used.
- Topic 1: Quick facts about NEFH. Neurofilament heavy polypeptide, or NEFH, is part of the neuronal cytoskeleton neurofilaments. It combines with neurofilament light and neurofilament medium chains to make the different neurofilaments throughout our body. NEFH is 1,026 amino acids long and runs about 200 kilodaltons on the Western blot.
- Topic 2: Where is NEFH expressed? Unsurprisingly, the highest levels of NEFH have been found in the brain, notably in the cerebellum, hippocampus, and cerebral cortex. Soft tissues also express high levels of NEFH. Male and female reproductive tissues express moderate levels of NEFH. There is little to no expression of NEFH in the remaining organs. You can find more information at protein atlas dot org, including which cell lines express NEFH and which ones don’t. This information will come in handy when designing positive and negative controls for western blot and immunohistochemistry.
- Topic 3: NEFH in Western blotting. Antibody specificity can make or break an experiment. After looking through Western blot images from publications and antibody companies, we can see that the band for the NEFH runs at around 200 kilodaltons. Check out the PMC link for more images, which is also provided in the description box.
- Topic 4: Some interesting facts about NEFH. As the protein name states, NEFH is responsible for making neurofilaments. It is an intermediate protein that combines with NEFL and NEFM to form the neurofilaments in our brain and spinal cord. These filaments are responsible for the intracellular transport of material to axons and dendrites. NEFH is also an important biomarker for neuronal damage. It is emerging as a critical gene in the development of amyotrophic lateral sclerosis. People who develop ALS have mutations in the NEFH gene. Thus, in the coming years, NEFH could become a key therapeutic target for ALS patients, in hopes of offering an improved quality of life or a potential cure for the disease.