Learn FLT1/VEGFR1 in 3 minutes | Fms-related tyrosine kinase 1
•Download as PPTX, PDF•
0 likes•68 views
Does your next experiment involve Fms-related tyrosine kinase 1 (FLT1)? This is a presentation about FLT1/VEGFR1 intended for scientists who are designing controls and performing immunoassays detecting FLT1/VEGFR1. It contains useful info such as Western blot band size, protein expression, and interesting facts. Anti-FLT1 Antibody Picoband™ (A00534-4): https://www.bosterbio.com/anti-flt1-picoband-trade-antibody-a00534-4-boster.html References: Uniprot.org, ProteinAtlas.org, PMC6594043, PMC4710184, PMC2615562 Learn more about FLT1/VEGFR1 (infographic and discussion): https://www.bosterbio.com/bosterbio-gene-info-cards/FLT1 Boster Biological Technology Website: www.bosterbio.com Email: support@bosterbio.com
Recommended
Recommended
More Related Content
Similar to Learn FLT1/VEGFR1 in 3 minutes | Fms-related tyrosine kinase 1
Similar to Learn FLT1/VEGFR1 in 3 minutes | Fms-related tyrosine kinase 1 (20)
More from Boster Biological Technology
More from Boster Biological Technology (14)
Recently uploaded
Recently uploaded (20)
Learn FLT1/VEGFR1 in 3 minutes | Fms-related tyrosine kinase 1
- 1. www.bosterbio.com FLT1 IN 3 MINUTES Quick facts, control designs, Western Blot MW. Info you can use to find the best FLT1 antibody.
- 2. FACTS 01. Function, structure, etc. TISSUES 02. Expressing tissues/cells WESTERN BLOT 03. Expected band sizes HISTORY 04. …and medical significance TABLE OF CONTENTS
- 3. ● Fms Related Receptor Tyrosine Kinase 1 (FLT1) ● Also called VEGFR-1 ● Cell-surface receptor for VEGFA, VEGFB, and PGF ● Involved in angiogenesis, cell survival, migration and other roles ● FLT1 is 1,338 amino acids long ● Protein size: ~151 kDa 01. FACTS AlphaFold predicted structure Image source: https://alphafold.ebi.ac.uk/entry/P17948
- 4. 02. TISSUES Image source: https://www.proteinatlas.org/ENSG00000102755-FLT1/tissue
- 5. 03. FLT1 WESTERN BLOT 69 images available on PMC: https://www.ncbi.nlm.nih.gov/pmc?term=FLT1+western+blot&report=imagesdocsum After reviewing several WB images, we observe FLT1 at ~170 kDa. PMC6594043 Figure 1 PMC2615562 Figure 2 Boster Bio FLT1 Antibody (A00534-4, Rabbit Polyclonal) PMC4710184 Figure 1
- 6. FLT1 (or VEGFR-1) was first noted to be a receptor for VEGF-A in 1992. 04. HISTORY High expression of the soluble splice variant of FLT1 (sFlt-1) has been implicated in Preeclampsia. 1992 CLINICAL POTENTIAL
- 7. 3942 B Valley Ave Pleasanton, CA 94566 Tel: (888) 466-3604 Email: support@bosterbio.com www.bosterbio.com
Editor's Notes
- Does your next experiment involve FLT1? This video is for scientists who want to run Western blot, IHC, flow or other immunoassays against FLT1.
- We will cover some basic facts, protein expression, and FLT1’s expected behavior in Western blot. All information in this video is based on public information and no proprietary experimental evidence was used.
- Topic 1: Quick facts about FLT1. Fms Related Receptor Tyrosine Kinase 1, or FLT1, is a cell surface receptor for VEGFA, VEGFB, and PGF. As such, it is highly involved in a number of processes such as angiogenesis, cell survival, cell migration, and many other crucial roles. FLT1 is 1,338 amino acids long with a molecular weight of about 151 kilodaltons.
- Topic 2: Where is FLT1? FLT1 is expressed highly in the lungs. FLT1 is also expressed in the placenta, testes, smooth muscle, skin, and colon. You can find more information on protein atlas dot org to check out which cell lines express FLT1 and which ones don’t. This information will come in handy when designing positive and negative controls for western blot and immunohistochemistry.
- Topic 3: FLT1 in Western blotting. Antibody specificity can make or break an experiment. After looking through Western blot images from publications and antibody companies, we can see that typically one band can be expected in a FLT1 western blot at about 170 kilodaltons. Check out the PMC link for more images, which is also provided in the description box.
- Topic 4: Some interesting facts about FLT1. FLT1, or VEGFR1, was first noted to be a receptor for VEGFA in 1992. Since then, research has implicated high expression of the soluble splice variant of FLT1 in the condition Preeclampsia.