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WBC & PLATELETS
ANOMALIES
PRINCESS ALEN AGUILAR
Toxic granulation

•
•
•
•

Dark blue-black cytoplasmic granules in neutrophil
Thought as primary granules
Show inc.alkaline phosphatase activity
Found in: acute infections
drug poisoning
burns
Dohle Bodies

• Single or multiple light blue or gray areas in cytoplasm of
neutrophils
• RER & represent failure of cytoplasm to mature
• Found in: infections
poisoning
burns
following chemotherapy
Hypersegmented Neutrophils

• Neutrophils with six or more lobed nucleus
• Represents an abnormality in maturation of neutrophil
• Acquired(in megaloblastic erythropoiesis) or inherited(Undritz
anomaly)
• Found in: pernicious anemia
folic acid deficiency
chronic infections
Barr Body

• Sex chromatin
• Represents the second X chromosome in females (23% of neutrophils in females)
• Small,well-defined,round projection of nuclear
chromatin
• These cells are not found in normal males.
Degenerated Neutrophil w/
pyknotic nucleus
• Result from condensing of
nuclear chromatin into a solid
structure mass with no
pattern
• Not counted in differential
cell count
Vacuolated neutrophil

• Degeneration of cytoplasm begins to acquire holes or
as result of active phagocytosis
• May reflect increased lysosomal activity
• Found in: septicemia
severe infection
Giant Neutrophils

• Can be seen occasionally in normal peripheral blood smear
• Larger than normal neutrophils and genrally
hyperlobulated
• Found in frequency of 1 in every 20,000 neutrophils but
increase in disease states
Pelger-Huet Anomaly
• Indicates failure of
neutrophil to segment
properly
• Bi-lobed nucleus;
chromatin is coarsely
clumped
• May be inherited or
acquired (as in leukemias)
• Heterozygous for this
char.shows numerous bilobed (dumbell shape);
homozygous-round
neutrophil
Chediak-Higashi Syndrome
(Autosomal recessive disorder)
• Rare,fatal disprder found in
children
• Inherited as an autosomal
recessive char.
• Contain very large,reddishpurple or greenish-gray staining
granules in the cytoplasm of
granulocytes
• In monocytes & lymphocytes,
stain bluish-purple
• These granules represent
abnormal lysosomes
• Found in: anemia
neutropenia
thrombocytopenia
Alder-Reilly anomaly
• Heavy,coarse blue-black
granules of BEN &
sometimes lymphocytes &
monocytes
• Inherited condition
• Associated with Hurler’s
syndrome & Hunter’s
syndrome
May-Hegglin Anomaly

• Inherited anomaly affecting neutrophils and platelets
• Larger than usual Dohle-like bodies
• Giant bizarre platelets is present & function may be
abnormal
Auer rods
• Rod-like bodies representing
aggregated primary granules
that stain reddish purple
• Found in : cytoplasm of
myeloblast, monoblast and
promyelocytes in acute
monocytic or acute
myelogenous leukemia and
eythroleukemia
Smudge or Basket cell

• Disintegrating nucleus of ruptured WBC
• Platelets encircling the peripheral borders of
neutrophils
• This phenomenon is thought to be due to a serum
factor which reacts in the presence of EDTA.

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Wbc & platelets anomalies

  • 2.
  • 3. Toxic granulation • • • • Dark blue-black cytoplasmic granules in neutrophil Thought as primary granules Show inc.alkaline phosphatase activity Found in: acute infections drug poisoning burns
  • 4. Dohle Bodies • Single or multiple light blue or gray areas in cytoplasm of neutrophils • RER & represent failure of cytoplasm to mature • Found in: infections poisoning burns following chemotherapy
  • 5. Hypersegmented Neutrophils • Neutrophils with six or more lobed nucleus • Represents an abnormality in maturation of neutrophil • Acquired(in megaloblastic erythropoiesis) or inherited(Undritz anomaly) • Found in: pernicious anemia folic acid deficiency chronic infections
  • 6. Barr Body • Sex chromatin • Represents the second X chromosome in females (23% of neutrophils in females) • Small,well-defined,round projection of nuclear chromatin • These cells are not found in normal males.
  • 7. Degenerated Neutrophil w/ pyknotic nucleus • Result from condensing of nuclear chromatin into a solid structure mass with no pattern • Not counted in differential cell count
  • 8. Vacuolated neutrophil • Degeneration of cytoplasm begins to acquire holes or as result of active phagocytosis • May reflect increased lysosomal activity • Found in: septicemia severe infection
  • 9. Giant Neutrophils • Can be seen occasionally in normal peripheral blood smear • Larger than normal neutrophils and genrally hyperlobulated • Found in frequency of 1 in every 20,000 neutrophils but increase in disease states
  • 10. Pelger-Huet Anomaly • Indicates failure of neutrophil to segment properly • Bi-lobed nucleus; chromatin is coarsely clumped • May be inherited or acquired (as in leukemias) • Heterozygous for this char.shows numerous bilobed (dumbell shape); homozygous-round neutrophil
  • 11. Chediak-Higashi Syndrome (Autosomal recessive disorder) • Rare,fatal disprder found in children • Inherited as an autosomal recessive char. • Contain very large,reddishpurple or greenish-gray staining granules in the cytoplasm of granulocytes • In monocytes & lymphocytes, stain bluish-purple • These granules represent abnormal lysosomes • Found in: anemia neutropenia thrombocytopenia
  • 12. Alder-Reilly anomaly • Heavy,coarse blue-black granules of BEN & sometimes lymphocytes & monocytes • Inherited condition • Associated with Hurler’s syndrome & Hunter’s syndrome
  • 13. May-Hegglin Anomaly • Inherited anomaly affecting neutrophils and platelets • Larger than usual Dohle-like bodies • Giant bizarre platelets is present & function may be abnormal
  • 14. Auer rods • Rod-like bodies representing aggregated primary granules that stain reddish purple • Found in : cytoplasm of myeloblast, monoblast and promyelocytes in acute monocytic or acute myelogenous leukemia and eythroleukemia
  • 15. Smudge or Basket cell • Disintegrating nucleus of ruptured WBC
  • 16. • Platelets encircling the peripheral borders of neutrophils • This phenomenon is thought to be due to a serum factor which reacts in the presence of EDTA.