This document summarizes different types of erythrocyte inclusions seen on supravital and Wright staining, along with their composition and associated disease states. It describes inclusions such as basophilic stippling composed of RNA seen in iron deficiency, Howell-Jolly bodies which are nuclear fragments seen in megaloblastic anemia, Heinz bodies containing denatured hemoglobin associated with G6PD deficiency, and siderotic granules containing iron found in sideroblastic anemia.

1. ERYTHROCYTE INCLUSIONS
RBC INCLUSION APPEARANCE IN SUPRAVITAL STAIN APPEARANCE IN WRIGHT STAIN INCLUSION COMPOSED OF ASSOCIATED DISEASE STATES
Diffuse basophilia Granulesandfilaments(reticulum)
Bluishtinge throughoutredcell;
polychromasia
Ribonucleicacid(RNA)
Hemolyticanemia
Aftertreatmentforirondeficiency
or megaloblasticanemia
Basophilicstippling
(punctate basophilia)
Granulesandfilaments(reticulum)
Blue-blackspecksdistributedthroughout
the cytoplasm
PrecipitatedRNA
Lead or heavymetal intoxication
Thalassemia
Aftertreatmentforirondeficiency
or megaloblasticanemia
Howell-Jollybody Dense,round,blue granule Dense,round,bluish-redgranule
Deoxyribonucleicacid(DNA) 
nuclearfragment– aberrant
chromosome
Megaloblasticanemia
Heinzbody Round, blue granule Notvisible (visibleinphase contrast) Denaturedhemoglobin
Glucose-6-phosphate
dehydrogenase(G6PDdeficiency)
Hb Zurich,Hb Ube, otherhereditary
hemolyticanemias
Sideroticgranules
(Pappenheimerbody)
Bluish-greenstaininginPrussian blue
reaction
Dark blue granule,oftennearperiphery Ferriciron
Sideroblasticanemia
Hemolyticanemia
Hyposplenism
Cabot ring Blue ringor figure of eightstrand Reddishringorfigure of eightstrand Remnantof mitoticspindle of nucleus
Pernicious anemia
Lead intoxication
Hb H crystals
Bluish-greengranulesinsupravital
stain
Notvisible Precipitate of βchains Hb H disease