UV-visible spectroscopy involves using electromagnetic radiation in the ultraviolet-visible spectral region to analyze chemical substances. It works by measuring how molecules absorb different wavelengths of visible and ultraviolet light. There are several techniques covered in the document, including UV-visible spectroscopy which analyzes electronic transitions in bonding electrons, and how it can be used to determine structural properties like conjugation and stereochemistry based on wavelength absorption maximum values. The document also outlines other spectroscopic techniques like infrared and NMR spectroscopy.
Uv-Vis spectroscopy: electronic spectroscopy, absorption and emission, Terms describing UV absorptions, absorbing species containing s,n and pi, absorbing species,sigma and pi orbitals, electronic transitions, Absorption: physical Basis and lineshape,UV-Spectra.
Uv-Vis spectroscopy: electronic spectroscopy, absorption and emission, Terms describing UV absorptions, absorbing species containing s,n and pi, absorbing species,sigma and pi orbitals, electronic transitions, Absorption: physical Basis and lineshape,UV-Spectra.
UV spectroscopy is an analytical method used to detct the numbers of double and triple bonds present in dienes ,trienes and polyenes compounds.The energy corresponds to EM radiation in the ultraviolet (UV) region, 100-350 nm, and visible (VIS) regions 350-700 nm of the spectrum is known as UV spectrum.
Applications of Infrared spectroscopy
Identification of organic compounds,
Structure determination
Qualitative analysis of functional group
Quantitative analysis
Distinction between two types of hydrogen bonding
Study of chemical reaction
Study of Keto-Enol tautomerism
Conformational analysis
Geometrical isomerism
Study of complex molecules
Detection of impurity in a compound
Identification of the organic compounds by IR
Hydrocarbons, Aromatic compounds, Alcohol, Phenols, Ethers, Aldehydes, Ketones, Esters, Acid chlorides, Anhydrides, Amides, Amines, Nitriles, Isocynates, Isothiocynates, Imines and Nitro compounds.
It is an analytical technique useful for the determination of molecular mass, molecular formula and fragmentation pattern of particular molecule and compounds. It has greater application in pharmaceutical and medicinal fields.
It is spectroscopy technique to determine number of hydrogen atoms present in the molecules and atoms.It is useful method for separation of molecules and compounds from mixtures components highly recommended in pharmaceutical and chemical engineering fields.
Introduction to Spectroscopy,
Introduction to UV, electronic transitions, terminology, chromophore, Auxochrome, Examples and Applications.
Introduction to IR, Fundamental vibrations, Types of Vibrations, Factors affecting the vibrational freaquencies, Group frequencies, examples and applications.
UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of different analytes, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Molecules containing bonding and non-bonding electrons undergo electronic transitions and absorb energy in the form of ultraviolet or visible light to excite these electrons to higher anti-bonding molecular orbitals.
UV spectroscopy is an analytical method used to detct the numbers of double and triple bonds present in dienes ,trienes and polyenes compounds.The energy corresponds to EM radiation in the ultraviolet (UV) region, 100-350 nm, and visible (VIS) regions 350-700 nm of the spectrum is known as UV spectrum.
Applications of Infrared spectroscopy
Identification of organic compounds,
Structure determination
Qualitative analysis of functional group
Quantitative analysis
Distinction between two types of hydrogen bonding
Study of chemical reaction
Study of Keto-Enol tautomerism
Conformational analysis
Geometrical isomerism
Study of complex molecules
Detection of impurity in a compound
Identification of the organic compounds by IR
Hydrocarbons, Aromatic compounds, Alcohol, Phenols, Ethers, Aldehydes, Ketones, Esters, Acid chlorides, Anhydrides, Amides, Amines, Nitriles, Isocynates, Isothiocynates, Imines and Nitro compounds.
It is an analytical technique useful for the determination of molecular mass, molecular formula and fragmentation pattern of particular molecule and compounds. It has greater application in pharmaceutical and medicinal fields.
It is spectroscopy technique to determine number of hydrogen atoms present in the molecules and atoms.It is useful method for separation of molecules and compounds from mixtures components highly recommended in pharmaceutical and chemical engineering fields.
Introduction to Spectroscopy,
Introduction to UV, electronic transitions, terminology, chromophore, Auxochrome, Examples and Applications.
Introduction to IR, Fundamental vibrations, Types of Vibrations, Factors affecting the vibrational freaquencies, Group frequencies, examples and applications.
UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of different analytes, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Molecules containing bonding and non-bonding electrons undergo electronic transitions and absorb energy in the form of ultraviolet or visible light to excite these electrons to higher anti-bonding molecular orbitals.
UV -Vis Spectrophotometry- Principle, Theory, Instrumentation and Application...Dr. Amsavel A
UV -Vis Spectrophotometry- Principle, Theory, Instrumentation and Application in Pharmaceutical Industry Dr. A. Amsavel.
UV &Visible Spectroscopy-Absorption Theory
Electronic Transitions
Beer- Lambert Law
Chromophores & Auxochrome
Factors Influence the Absorption
UV-Vis Spectrophotometer-Instrumentation
Operation of the Spectrophotometer
Qualification & Calibration
Application
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
1. UV Visible spectroscopy
By
Mr. G. P. Sadawarte
Asst. Professor,Dept. of Chemistry
B. P. Arts, S.M.A. Sci. and K.K.C. Comm. College
chalisgaon
B.P. Arts, S.M.A. Science And K.K.C.
Commerce College, Chalisgaon,
3. Wave parameter
Frequency (V) – the number of cycles/wave which pass a
fixed point in space per second.
Amplitude (A) – The maximum length of the electric vector
in the wave from X axis (Maximum height of a wave).
Wavelength (λ) – The distance between two successive
crest/troughs adjacent points in a wave (usually maxima or
minima).
Wavenumber- The number of waves per cm in units of cm-1.
4. Energy
• EM is viewed as a stream of discrete particles, or wave
packets, of energy called photons.
• We can relate the energy of photon to its wavelength,
frequency and wavenumber by
E = hV V - frequency
= h c
λ λ - wavelength
= hcυ
h – Planck’s constant =6.63x10-34 J·s
Energy inversely proportional to Wavelength
6. Spectroscopic Techniques and
Chemistry they Probe
UV-vis UV-vis region bonding electrons
Atomic Absorption UV-vis region atomic transitions (val. e-)
FT-IR IR/Microwave vibrations, rotations
Raman IR/UV vibrations
FT-NMR Radio waves nuclear spin states
X-Ray Spectroscopy X-rays inner electrons, elemental
X-ray Crystallography X-rays 3-D structure
7. Different Spectroscopies
• UV-vis – electronic states of valence e/d-orbital
transitions for solvated transition metals
• Fluorescence – emission of UV/vis by certain molecules
• FT-IR – vibrational transitions of molecules
• FT-NMR – nuclear spin transitions
• X-Ray Spectroscopy – electronic transitions of core
electrons
8. Molecular transition
• In molecules the electronic states are subdivided into
vibrational states.
• The energy of a band in a molecular absorption
spectrum is the sum of three different energy
components.
• E = Eelectronic + Evibrational + Erotational
• Transitions between electronic-vibrational-rotational
states give rise to spectra that appear to have bands.
9. Lambert Beer’s Law
cuvette
source
slit
detector
log(I0/I) = ϵ.l.c
I0 = intensity of incident light
I = intensity of Transmitted light
c = Concentration of sample
l = Path length of sample
ϵ = Molar absorptivity
The fraction of incident
light absorbed is
proportional to no of
absorbing molecule in its
path
10. Light source and detector
REGION SOURCE SAMPLE
HOLDER
DETECTOR
Ultraviolet Deuterium lamp Quartz/fused
silica
Phototube, PM
tube, diode array
Visible Tungsten lamp Glass/quartz Phototube, PM
tube, diode array
Infrared Nernst glower (rare earth
oxides or silicon carbide
glowers)
Salt crystals e.g.
crystalline
sodium chloride
Thermocouples,
bolometers
13. Auxochrome
• Saturated functional group which does not
absorbs UV radiation at longer wavelength.
• When its is connected to Chromophore it
changes both wavelength and intensity
• OH, NH2, Cl,
Benzene (255 nm)
OH
Phenol (270 nm)
NH2
Aniline (280 nm)
14. Bathochromic shift
• Shift in absorption to longer wavelength
• Red shift
• ˄ max increses
OH
• Phenol (270nm) • Phenoxide ion (287 nm)
O
P Nitro phenol (320) and Phenoxide of it (400)
15. Hypsochromic Shift
• Shift in absorption to shorter wavelength
• Blue shift
• ˄ max decreases
Aniline (280nm ) Aniline hydrochloride (203 nm)
NH2
NH3Cl
16. Woodward Fischer Rule
• Homoannular Diene -Both double bonds are
conjugation in same ring
• S-cis or cisoid
• Base value ʎ max 253 nm
17. Woodward Fischer Rule
• Heteroannular Diene-double bonds are in
conjugation Present two different ring
• S-Trans or Transoid
• Base value ʎ max 215 nm
Heteroannular
18. Woodward Fischer Rule
Exocyclic double bond
Double bond projected to outside ring Add 5 nm
For each Alkyl Substituent or Ring residue add 5 nm
Extra DB Which increases Conjugation =30 nm
Exocyclic DB
24. Woodward Fischer Rule –enone system
• Base value for cyclic/ six membered enone 215nm
• Base value for cyclic Five membered enone 202nm
• Base value for aldehyde 207nm
• Base value for acid /ester 197nm
• Extra DB which increases Conjugation 30 nm
• Homodiene Componant 39 nm
• Exocyclic DB 05nm
29. Application of UV Visible
• Structure determination – presence or absence of
Chromophore and Conjugation
• Determination of steriochemistry-
Cis stilbene λ Max 280nm
Trans stilbene λ max 296nm
• Strength of Hydrogen Bond –
Acetone in n-Hexane 297nm
Acetone in water 264nm
30. References
• Introduction to Spectroscopy, Donald L. Pavia Gary
M. Lampman George S. Kriz James R. Vyvyan,
• Spectroscopy by Y.R. Sharma
• Spectrometric Identification of Organic
Compounds, R. M. Silverstein and F. X. Webster ,
John Wiley and Sons