- The Amur tiger population declined to 30 individuals in the 1940s and is now critically endangered. Two subpopulations exist separated by a development corridor, but genetic testing found no significant differentiation between them.
- Testing found no evidence of a recent genetic bottleneck and signs of population expansion that explain the lack of bottleneck signature. The effective population size is estimated to be around 35 individuals.
- The captive breeding program maintained genetic diversity representative of the wild population, though 3 alleles were found in captivity not in the wild. Conservation measures must continue both in situ and ex situ to ensure the species' survival from human and environmental threats given its low population size.
R and k selection is very important aspect of knowing ecology a better way. This is based on natural selection of species (that is stable condition leads to k selection & unstable one leads to r selection).
hardy weinberg genetic equilibrium by kk sahuKAUSHAL SAHU
INTRODUCTION
HISTORY
THE HARDY-WEINBERG LAW
DERIVATION
TERMINOLOGY
PROBLEMS
ASSUMPTION OF HAEDY –WEINBERG EQUILIBRIUM
REFERANCE
The Hardy–Weinberg principle, also known as the Hardy–Weinberg equilibrium, model, theorem or law.
States that allele and genotype frequencies in a population will remain constant from generation to generation in the absence of other evolutionary influences.
These influences include mate choice, mutation, selection, genetic drift, gene flow and meiotic drive.
Because one or more of these influences are typically present in real populations, the Hardy–Weinberg principle describes an ideal condition against which the effects of these influences can be analyzed.
Ppt is made vailable for public for scientifc use.
Population ecology concept and its characteristics explained by using practical examples in a simple language. data is significant for competitive examinations
R and k selection is very important aspect of knowing ecology a better way. This is based on natural selection of species (that is stable condition leads to k selection & unstable one leads to r selection).
hardy weinberg genetic equilibrium by kk sahuKAUSHAL SAHU
INTRODUCTION
HISTORY
THE HARDY-WEINBERG LAW
DERIVATION
TERMINOLOGY
PROBLEMS
ASSUMPTION OF HAEDY –WEINBERG EQUILIBRIUM
REFERANCE
The Hardy–Weinberg principle, also known as the Hardy–Weinberg equilibrium, model, theorem or law.
States that allele and genotype frequencies in a population will remain constant from generation to generation in the absence of other evolutionary influences.
These influences include mate choice, mutation, selection, genetic drift, gene flow and meiotic drive.
Because one or more of these influences are typically present in real populations, the Hardy–Weinberg principle describes an ideal condition against which the effects of these influences can be analyzed.
Ppt is made vailable for public for scientifc use.
Population ecology concept and its characteristics explained by using practical examples in a simple language. data is significant for competitive examinations
Presentation delivered by Dr. Jesse Poland (Kansas State University, USA) at Borlaug Summit on Wheat for Food Security. March 25 - 28, 2014, Ciudad Obregon, Mexico.
http://www.borlaug100.org
It is as per the syllabus of M.Sc. NRM including detailed study of population ecology
It describes the meaning of population with respect to ecology and includes population attributes, dynamics, dispersal, Population growth models, survivorship curves and limitations.
It also entails factors that influence and regulate population growth on the basis of density.
It is as per the syllabus of M.Sc. NRM including detailed study of population ecology
It describes the meaning of population with respect to ecology and includes population attributes, dynamics, dispersal, Population growth models, survivorship curves and limitations.
It also entails factors that influence and regulate population growth on the basis of density.
Bats of the Channel Islands: Using Mathematics to Protect our Elusive Noctur...Jason Miller
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Human genetic diversity and origin of major human groupsMayank Sagar
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"Understanding the Carbon Cycle: Processes, Human Impacts, and Strategies for...MMariSelvam4
The carbon cycle is a critical component of Earth's environmental system, governing the movement and transformation of carbon through various reservoirs, including the atmosphere, oceans, soil, and living organisms. This complex cycle involves several key processes such as photosynthesis, respiration, decomposition, and carbon sequestration, each contributing to the regulation of carbon levels on the planet.
Human activities, particularly fossil fuel combustion and deforestation, have significantly altered the natural carbon cycle, leading to increased atmospheric carbon dioxide concentrations and driving climate change. Understanding the intricacies of the carbon cycle is essential for assessing the impacts of these changes and developing effective mitigation strategies.
By studying the carbon cycle, scientists can identify carbon sources and sinks, measure carbon fluxes, and predict future trends. This knowledge is crucial for crafting policies aimed at reducing carbon emissions, enhancing carbon storage, and promoting sustainable practices. The carbon cycle's interplay with climate systems, ecosystems, and human activities underscores its importance in maintaining a stable and healthy planet.
In-depth exploration of the carbon cycle reveals the delicate balance required to sustain life and the urgent need to address anthropogenic influences. Through research, education, and policy, we can work towards restoring equilibrium in the carbon cycle and ensuring a sustainable future for generations to come.
different Modes of Insect Plant InteractionArchita Das
different modes of interaction between insects and plants including mutualism, commensalism, antagonism, Pairwise and diffuse coevolution, Plant defenses, how coevolution started
WRI’s brand new “Food Service Playbook for Promoting Sustainable Food Choices” gives food service operators the very latest strategies for creating dining environments that empower consumers to choose sustainable, plant-rich dishes. This research builds off our first guide for food service, now with industry experience and insights from nearly 350 academic trials.
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Improving the viability of probiotics by encapsulation methods for developmen...Open Access Research Paper
The popularity of functional foods among scientists and common people has been increasing day by day. Awareness and modernization make the consumer think better regarding food and nutrition. Now a day’s individual knows very well about the relation between food consumption and disease prevalence. Humans have a diversity of microbes in the gut that together form the gut microflora. Probiotics are the health-promoting live microbial cells improve host health through gut and brain connection and fighting against harmful bacteria. Bifidobacterium and Lactobacillus are the two bacterial genera which are considered to be probiotic. These good bacteria are facing challenges of viability. There are so many factors such as sensitivity to heat, pH, acidity, osmotic effect, mechanical shear, chemical components, freezing and storage time as well which affects the viability of probiotics in the dairy food matrix as well as in the gut. Multiple efforts have been done in the past and ongoing in present for these beneficial microbial population stability until their destination in the gut. One of a useful technique known as microencapsulation makes the probiotic effective in the diversified conditions and maintain these microbe’s community to the optimum level for achieving targeted benefits. Dairy products are found to be an ideal vehicle for probiotic incorporation. It has been seen that the encapsulated microbial cells show higher viability than the free cells in different processing and storage conditions as well as against bile salts in the gut. They make the food functional when incorporated, without affecting the product sensory characteristics.
Epcon is One of the World's leading Manufacturing Companies.EpconLP
Epcon is One of the World's leading Manufacturing Companies. With over 4000 installations worldwide, EPCON has been pioneering new techniques since 1977 that have become industry standards now. Founded in 1977, Epcon has grown from a one-man operation to a global leader in developing and manufacturing innovative air pollution control technology and industrial heating equipment.
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Improving the Management of Peatlands and the Capacities of Stakeholders in I...
Tiger population genetics
1. In situ and ex situ conservation
genetics of the endangered Amur tiger
P. Henry *, T. Sugimoto, D. Miquelle °, A. Caccone ‡
& M. Russello *
*
°
‡
2. The Amur Tiger: Background
• Panthera tigris altaica
• Population decline to 30 individuals in the
1940’s
• Critically endangered (IUCN red list)
• In situ conservation strategies
• Captive breeding program
3. The Amur Tiger: Background
• Panthera tigris altaica
• Population decline to 30 individuals in the
1940’s
• Critically endangered (IUCN red list)
• In situ conservation strategies
• Captive breeding program
4. The Amur Tiger: Background
• Panthera tigris altaica
• Population decline to 30 individuals in the
1940’s
• Critically endangered (IUCN red list)
• In situ conservation strategies
• Captive breeding program
5. The Amur Tiger: Background
• Panthera tigris altaica
• Population decline to 30 individuals in the
1940’s
• Critically endangered (IUCN red list)
• In situ conservation strategies
• Captive breeding program
6. The Amur Tiger: Background
• Panthera tigris altaica
• Population decline to 30 individuals in the
1940’s
• Critically endangered (IUCN red list)
• In situ conservation strategies
• Captive breeding program
8. Present distribution
•Now mainly found
in:
- RFE
- Small areas of
China & North
Korea
• Estimated 490
individuals left in
the wild
9. Samples
• Wild samples:
112 scat samples
collected throughout
current range
representing 95
individuals
• Captive samples:
12 individuals from
captive population
(North American
Zoos)
10. Samples
• Wild samples:
112 scat samples
collected throughout
current range
representing 95
individual
• Captive samples:
12 individuals from
captive population
(North American
Zoos)
13. Objectives
• Investigate population genetic structure
and demography of in situ population
• Assess the genetic representation of the
in situ population in captivity
14. Objectives
• Investigate population genetic structure
and demography of in situ population
• Assess the genetic representation of the
in situ population in captivity
15. Population genetic structure
• Two hypothesized
subpopulations
separated by a
development corridor
• All test of
differentiation were not
significant (FST=0.0491,
RST=0.0272)
Sikhote Alin SW Primorye
16. Population genetic structure
• Two hypothesized
subpopulations
separated by a
development corridor
• All test of
differentiation were not
significant (FST=0.0491,
RST=0.0272)
Sikhote Alin SW Primorye
17. • There is no evidence for recent bottleneck on
three commonly used tests for bottleneck.
(Luikart et al. 1998 + Garza & Williamson 2001)
• Tests for population expansion indicate
significant expansion. (Reich et al. 1999)
• Estimates of effective population vary from
22 to 57 individuals with a mean estimate of
35 individuals (Tallmon et al. 2008)
Demography in the wild
18. • There is no evidence for recent bottleneck on
three commonly used tests for bottleneck.
(Luikart et al. 1998 + Garza & Williamson 2001)
• Tests for population expansion indicate
significant expansion. (Reich et al. 1999)
• Estimates of effective population vary from
22 to 57 individuals with a mean estimate of
35 individuals (Tallmon et al. 2008)
Demography in the wild
19. • There is no evidence for recent bottleneck on
three commonly used tests for bottleneck.
(Luikart et al. 1998 + Garza & Williamson 2001)
• Tests for population expansion indicate
significant expansion. (Reich et al. 1999)
• Estimates of effective population vary from
22 to 57 individuals with a mean estimate of
35 individuals (Tallmon et al. 2008)
Demography in the wild
20. • Genetic diversity
not significantly
different
• No significant
differentiation
(FST= 0.0496, RST=
0.0235)
• 3 private alleles
detected ex situ
Genetic representation ex situ
21. • Genetic diversity
not significantly
different
• No significant
differentiation
(FST= 0.0496, RST=
0.0235)
• 3 private alleles
detected ex situ
Genetic representation ex situ
Axe 1 (10.51%)
22. • Genetic diversity
not significantly
different
• No significant
differentiation
(FST= 0.0496, RST=
0.0235)
• 3 private alleles
detected ex situ
Genetic representation ex situ
23. • Population structure and demography in situ:
- The development corridor does not represent a
barrier to gene flow between the Sikhote Alin and
SW Primorye
- Population expansion may be responsible for
the lack of genetic signature of a bottleneck
- Effective population size is 10 X smaller than
Significance of results
24. • Population structure and demography in situ:
- The development corridor does not represent a
barrier to gene flow between the Sikhote Alin and
SW Primorye
- Population expansion may be responsible for
the lack of genetic signature of a bottleneck
- Effective population size is 10 X smaller than
Significance of results
25. • Population structure and demography in situ:
- The development corridor does not represent a
barrier to gene flow between the Sikhote Alin and
SW Primorye
- Population expansion may be responsible for
the lack of genetic signature of a bottleneck
- Effective population size is 10 X smaller than
Significance of results
26. • Genetic representation ex situ
- Ex situ sample is representative of the genetic
variation found in situ
- Some genetic resources (3 alleles) found ex situ
were absent in situ
Significance of results
27. • Genetic representation ex situ
- Ex situ sample is representative of the genetic
variation found in situ
- Some genetic resources (3 alleles) found ex situ
were absent in situ
Significance of results
28. • The captive breeding program was
successful in maintaining a representative
sample of the wild population
• Conservation measures in situ will need to
be continued to ensure the survival of the
species as it may be vulnerable to continued
human-mediated stresses as well as
environmental stochasticity
Concluding remarks
29. • The captive breeding program was
successful in maintaining a representative
sample of the wild population
• Conservation measures in situ will need to
be continued to ensure the survival of the
species as it may be vulnerable to continued
human-mediated stresses as well as
environmental stochasticity
Concluding remarks
30. • Students:
Rebecca Catapano-Friedman (YALE)
Lisa Young (YALE)
• Helpful discussions:
Anders Gonçalves da Silva (UBC | O)
Jérôme Goudet (UNIL)
• Photo credit:
Elvis Payne
Tobi British
Ashley Darby
• Collaborators:
A. A. Astafiev (Sihkote-Alin Zapovednik)
Y. M. Dunishenko, E. N. Smirnov, G. Alkina, V. G. Abramov, D. G. Pikuno
(Amur Tiger Monitoring program)
John Goodrich (WCS)
Kathy Traylor-Holzer (CBSG)
Acknowledgements
• Funding:
WCS Species Survival Fund
Conservation Award (MR)
American Philosophical Society
(MR)
Christensen Fund (DM)
Good morning to you all. I am Philippe Henry and my talk today will be on the conservation genetics of the Amur tiger of both the insitu and exsitu populations.
This study constitute a side project of my PhD at the University of British Columbia Okanagan with Prof. Michael Russello and is done in collaboration with Dr. Taro Sugimoto, Dr. Dale Miquelle of the Wildlife Conservation Society and Prof. Adalgisa Caccone at Yale University.
The amur tiger (PTA) is the largest of all tiger subspecies.
Five out of eight subspecies of tigers are still extant. (bengal-tigris, sumatran-sumarae, indichinese- corbetti, amur-altaica & malay- jacksoni)
Extinct: Javan-sondaica, bali- balica& caspian-virgata / south-china-amoyensis--only in zoos
The wild population of amur tigers declined to about 30 individuals in the 1940’s due to poaching for asian medicine market and habitat destruction: due to logging and increased pressure from human populations
The subspecies is still critically endangered as pressures from logging persist and poaching for chinese medicine is still happening.
Conservation action in the the Russian Far east and China such as anti-poaching, human-tiger conflict mitigation, education and habitat management work has enabled the wild population to rebound toan estimated 490 individuals at present.
This was supplemented by a captive breeding program that was initiated in 1950’s from 57 wild caught amur tigers.
The captive population is now represented by 420 individuals in zoos and farms in china, europe and north america.
The amur tiger was previously sistributed in large areas in eastern chine, the korean peninsula as well as the russian far east.
Due to the pressures i mentionned before (Loggin, poaching for chinese medicinal practices, expansion of human population) it is now restricted to temperate forests of the RFE as well as small areas in neigboring china and north korea. The estimated individuals left in the wild is 490…
Inthe present study we collected scat samples from the entire current range of the amur tiger.
That is from two hypothesized subpopulations: a small subpopulation in The South-West Primorye (40 individuals) & the main subpopulation in the Sikhote Alin mountains in the east (450 individuals) i.e. a total of 490 individuals.
and we also had a sample of 12 captive individuals representing the remaining matriline from the 57 founders of the captive breeeding program (29 females and 28 males!!!) from Omaha, Bronx, Minnesota, Philadelphia, WCS (New York?) , Louisville
Based on the tiger studbooks.
We genotyped all individuals at 8 Microsatellite loci initially developped for sumatran tiger. (Williamson et al 2002 mol ecol notes)
And this was supplemented by mtDNA control region sequences. Haplotypes 3 in the wild one in captivity
With the samples available, we investigated the population genetic structure and demography in the wild
And we assessed how well the captive population was a genetic representation of the wild population
It has been hypothesized that a development corridor between the cities of Vladivostok and Ussurisk that has been ongoing for the past 20 years may limit geneflow between SW primorye and the Sikhote Alin moutains.
We tested this using several approaches: including standard Fst Rst measures of population differentiation as well a log-likelyhood test statistic G (Goudet e al 1996). And the bayesian approach implemented in the software structure.
All lines of evidence, ie low values of Fst and Rst and the log-likelyhood test statistic G were all non significant,
Here i have given you a structure plot also suggests that there is no evidence for population differentiation accross the corridor.
Q- HWE assumption._2 out of 8 loci deviated from HWE after sequential bonferroni corrections (Rice 1989). Those were removed for the structure analyses. The log-likelyhood G-test was undertaken not assuming the HWE with all loci.
Although a severe bottleneck event (20-30 individuals remaining) is well documented (Kaplanov 1948) there is no genetic evidence for a population crash based pn 3 commonly used tests:
BOTTLENECK: heterozygote excess test (based on the wilkoxon sign rank test- most powerful for a small number of loci), mode-shift (no distortion in allele frequencies)
& M-RATIO (large and never fell bellow the critical value Mc!)
Q_ Spong, G. and L. Hellborg. 2002. A near-extinction event in lynx: do microsatellite data tell the tale? Conservation Ecology 6(1): 15.
Suggest, low number of loci, undetected migration event, short, relatively narrow botlleneck may not leave any genetic signal,
Based on the intralocus k- test. suggest population expansion , on the other hand another test proposed, the interlocus variance g- tests described in Reich et al 1999 does not follow this evidence
Q_Based on the intralocus kurtosis k-tests (one-t ailed binomial test to deter mine whether fewer loci were associated with a positive k than would be expected for a constant-sized population- p<0.05) Because the expectation of k decreases with increasing kurtosis, such a reduction in the number of positive k values can be interpreted as a sign of population expansion. ---kurtosis=peakedness,
interlocus variance g test - the variance of the variance of the allele length distributions
Third, the within locus kurtosis test is designed to detect population expansions (Reich & Goldstein 1998; Reich et al. 1999) based on the distribution of allele sizes. The test is based on the observation that the allele distributions of an expanding population and that of a population that has been stable for a long time differ. In an expanding population the kurtosis (k), or rather a combination of the variance and kurtosis (Reich et al. 1999), of the allele size distribution is positive. The method uses a binomial test of the number of positive k-values based on the expectation of an almost (P = 0.515) equal probability of negative and positive k-values.
Fourth, the variance test is an interlocus test which tests the observation that in stable populations the variance of the allele sizes is highly variable among loci, whereas in an expanding population this variance is more even. Thus, sufficiently low variances in allele sizes may be taken as evidence for an expansion, and we used cut-off values given in Reich et al. (1999).
Q_ Spong, G. and L. Hellborg. 2002. A near-extinction event in lynx: do microsatellite data tell the tale? Conservation Ecology 6(1): 15.
Suggest, low number of loci, undetected migration event, short, relatively narrow botlleneck may not leave any genetic signal,
Onesamp 95% confidence interval (lower and upper bounds) with a mean at 35 inds… this value is backed up by the method of Guo and Waples based on Linkage disequilibrium with a estimates ranging from 20 to 61 individuals.
Q _Onesamp, which uses summary statistics and approximate Bayesian computation to estimate Ne from a single sample.
8 summary stats with established a relationship with Ne: 1. number of alleles divided by allele length range, 2. the difference of the natural logarithms of variance in allele length and heterozygosity, 3. expected heterozygosity, 4. number of alleles per locus, 5. Wrightユs F Is, 6&7. mean and variance of multilocus homozygosity, 8. square of the correlation of alleles at different loci.
note the test of Robin Wapples LDNe was also used and yielded 61 individuals (i.e. )
Q_ Spong, G. and L. Hellborg. 2002. A near-extinction event in lynx: do microsatellite data tell the tale? Conservation Ecology 6(1): 15.
Suggest, low number of loci, undetected migration event, short, relatively narrow botlleneck may not leave any genetic signal,
Genetic diversity were relatively low, but in accordance to previously published work on captive and wild amur tiger (Luo et al 2008) measured as Hs- expected heterozygosity (0.3-0.4) and Rs (about 3)- Allelic Richness (el mousadik & petit 1996) were no significantly different in ex situ and in situ samples. (the same applies to Fis!!! Although it is a bit higher in situ)
I gave you the example of Hs: one can see the exsitu sample has a similar range
Based on Fst, Rst and the loglikelyhood Gtest, the in situ and ex situ are not significantly differentiated
I have illustrated this by showing you the results of a factorial correspondans analysis, where the blue squares represent the captive individuals and the yellow represnt the wild individuals.
Three alleles found in captivity were absent from the wild: two at locus 6HDZ64 and one at locus 610
The development corridor does not barrier to gene flow but as it has this corridor is only about 20 years old, it may be too recent for its effect to be detected at the genetic level yet.
Popualtion expansion after the bottleneck may be responssible for the lack of genetic signature of bottleneck…
Other reason could include; the low number of loci used in this study…
undetected migration (unlikely…)
the fact that the 1940s bottleneck was short in time and the population was able to rebound quickly and attain equilibrium.
The effective population is more than ten times smaller the census size. This is important to take into consideration for future conservation action
Based on our sample of 12 individuals
Based on our sampling: (fairly low 12 individuals = 12 matrilines)
Due to a low effective population size identified here, it is waranted to continue in situ conservation strategies that are already in palce and:
Ultimately may benefit from an interactive ex situ-in situ management stratergie that could supplement genetic variability from the captive into the wild population by means of assisted reproductive technologies and/or transplanto f candidate individuals.
We are grateful to the following:
Students for conducting labwork.
insightful discussions
Teams on the field for collection of scats
Photo credit
collaborators
Funding sources