In Hypothesis testing parametric test is very important. in this ppt you can understand all types of parametric test with assumptions which covers Types of parametric, Z-test, T-test, ANOVA, F-test, Chi-Square test, Meaning of parametric, Fisher, one-sample z-test, Two-sample z-test, Analysis of Variance, two-way ANOVA.
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In Hypothesis testing parametric test is very important. in this ppt you can understand all types of parametric test with assumptions which covers Types of parametric, Z-test, T-test, ANOVA, F-test, Chi-Square test, Meaning of parametric, Fisher, one-sample z-test, Two-sample z-test, Analysis of Variance, two-way ANOVA.
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Through this ppt you could learn what is Wilcoxon Signed Ranked Test. This will teach you the condition and criteria where it can be run and the way to use the test.
Standard error is used in the place of deviation. it shows the variations among sample is correlate to sampling error. list of formula used for standard error for different statistics and applications of tests of significance in biological sciences
Through this ppt you could learn what is Wilcoxon Signed Ranked Test. This will teach you the condition and criteria where it can be run and the way to use the test.
Standard error is used in the place of deviation. it shows the variations among sample is correlate to sampling error. list of formula used for standard error for different statistics and applications of tests of significance in biological sciences
WEEK 6 – EXERCISES Enter your answers in the spaces pr.docxwendolynhalbert
WEEK 6 – EXERCISES
Enter your answers in the spaces provided. Save the file using your last name as the beginning of the file name (e.g., ruf_week6_exercises) and submit via “Assignments.” When appropriate,
show your work
. You can do the work by hand, scan/take a digital picture, and attach that file with your work.
1
.
A psychotherapist studied whether his clients self-disclosed more while sitting in an easy chair or lying down on a couch. All clients had previously agreed to allow the sessions to be videotaped for research purposes. The therapist randomly assigned 10 clients to each condition. The third session for each client was videotaped and an independent observer counted the clients’ disclosures. The therapist reported that “clients made more disclosures when sitting in easy chairs (
M
= 18.20) than when lying down on a couch (
M
= 14.31),
t
(18) = 2.84,
p
< .05, two-tailed.” Explain these results to a person who understands the
t
test for a single sample but knows nothing about the
t
test for independent means.
2.
A researcher compared the adjustment of adolescents who had been raised in homes that were either very structured or unstructured. Thirty adolescents from each type of family completed an adjustment inventory. The results are reported in the table below. Explain these results to a person who understands the
t
test for a single sample but knows nothing about the
t
test for independent means.
Means on Four Adjustment Scales for
Adolescents from Structured versus Unstructured Homes
Scale
Structured Homes
Unstructured Homes
t
Social Maturity
106.82
113.94
–1.07
School Adjustment
116.31
107.22
2.03*
Identity Development
89.48
94.32
1.93*
Intimacy Development
102.25
104.33
.32
______________________
*
p
< .05
3.
Do men with higher levels of a particular hormone show higher levels of assertiveness? Levels of this hormone were tested in 100 men. The top 10 and the bottom 10 were selected for the study. All participants took part in a laboratory simulation in which they were asked to role-play a person picking his car up from a mechanic’s shop. The simulation was videotaped and later judged by independent raters on each of four types of assertive statements made by the participant. The results are shown in the table below. Explain these results to a person who fully understands the
t
test for a single sample but knows nothing about the
t
test for independent means.
Mean Number of Assertive Statements
Type of Assertive Statement
Group
1
2
3
4
Men with High Levels
2.14
1.16
3.83
0.14
Men with Low Levels
1.21
1.32
2.33
0.38
t
3.81**
0.89
2.03*
0.58
______________________
*
p
< .05;
**
p
< 0.1
4.
A manager of a small store wanted to discourage shoplifters by putting signs around the store saying “Shoplifting is a crime!” However, he wanted to make sure this would not result in customers buying less. To test this, he displayed the signs every other W.
TEST #1Perform the following two-tailed hypothesis test, using a.docxmattinsonjanel
TEST #1
Perform the following two-tailed hypothesis test, using a .05 significance level:
· Intrinsic by Gender
· State the null and an alternate statement for the test
· Use Microsoft Excel (Data Analysis Tools) to process your data and run the appropriate test. Copy and paste the results of the output to your report in Microsoft Word.
· Identify the significance level, the test statistic, and the critical value.
· State whether you are rejecting or failing to reject the null hypothesis statement.
· Explain how the results could be used by the manager of the company.
TEST #2
Perform the following two-tailed hypothesis test, using a .05 significance level:
· Extrinsic variable by Position Type
· State the null and an alternate statement for the test
· Use Microsoft Excel (Data Analysis Tools) to process your data and run the appropriate test.
· Copy and paste the results of the output to your report in Microsoft Word.
· Identify the significance level, the test statistic, and the critical value.
· State whether you are rejecting or failing to reject the null hypothesis statement.
· Explain how the results could be used by the manager of the company.
GENERAL ANALYSIS (Research Required)
Using your textbook or other appropriate college-level resources:
· Explain when to use a t-test and when to use a z-test. Explore the differences.
· Discuss why samples are used instead of populations.
The report should be well written and should flow well with no grammatical errors. It should include proper citation in APA formatting in both the in-text and reference pages and include a title page, be double-spaced, and in Times New Roman, 12-point font. APA formatting is necessary to ensure academic honesty.
Be sure to provide references in APA format for any resource you may use to support your answers.
Making Inferences
When data are collected, various summary statistics and graphs can be used for describing data; however, learning about what the data mean is where the power of statistics starts. For example, is there really a difference between two leading cola products? Hypothesis testing is an example of making these types of inferences on data sets.
Hypothesis Tests
Claims are made all the time, such as a particular light bulb will last a certain number of hours.
Claims like this are tested with hypothesis testing. It is a straight forward procedure that consists of the following steps:
1. A claim is made.
2. A value for probability of significance is chosen.
3. Data are collected.
4. The test is performed.
5. The results are analyzed.
Hypothesis tests are performed on the mean of the population. µ
It is not possible to test the full population. For example, it would be impossible to test every light bulb. Instead, the hypothesis test is performed on a sample of the population.
Setting up a Hypothesis Test
When performing hypothesis testing, the test is setup with a null hypothesis (or claim) and the alternative hypothesis. ...
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
1. RAK college of Nursing
RAK Medical and Health Sciences University
Seminar on: T-test, independent and paired samples
Course: Statistics for health professionals
Prepared by:
Abdelrahman Alkilani- 15906012
Sultan Sultan- 15906013
Submitted to Dr. Maragatham Kannan, Associate professor
Date of submission: 15/11/2015
2. Objectives
By the end of this seminar, you’ll be able to:
Define T-test
Discuss the merits and demerits of t-test
Identify the independent t-test and discuss
when to use it.
Identify the paired t-test and discuss when to
use it.
Use the correct formulas of t-test
Use the t-test through SPSS
3. T-test
The t-test assesses whether the means of
two groups are statistically different from
each other
4. Merits and demerits
Merits:
– It reduces the possibility of guising the correct
answer .
– It covers greater amount of contents than
matching types test.
Demerits
– it is only appropriate for questions that can be
answered by short responses
– There is a difficult in scoring when the questions
are not prepared properly and clearly
5. Independent t-test
It is a parametric test used to determine if a
difference exists in the means of two groups
on a particular characteristic.
6. Independent t-test
Used when:
– The grouping variables is dichotomous
– The variable measuring the characteristics of
interest is normally distributed.
– The variable measuring the characteristic of
interest in interval or ratio
– The measure of each value of the variable, which
measures the characteristic of interest, constitute
and independent random sample
7. Computing the independent t-test
Need two t-values:
– Calculated t-value
– Critical t-value
If calculated t-value is greater than critical t-
value, then reject the null hypothesis.
8. Computing the independent t-test
Example:
In a study, we want to know whether smokers and
non-smokers have equal brain sizes.
10. Computing the independent t-test
1. State the null and alternative hypothesis.
H0 : There will be no difference in the means of the two
groups.
“smokers and non-smokers have equal brain size”
HA : smokers and non-smokers have unequal brain size
23. Paired t-test
The measurements of the same variable at
two different points are compared.
It can be measured at the same time on two
different people who are matched on some
condition (e.g. age, gender, twins).
24. Paired t-test
Used when:
– There are two measurements of characteristic of
interest.
– The two measures that are compared are
normally distributed or at least 30 pairs.
– The measurement scale is either interval or ratio.
25. Computing the paired t-test
Need two t-values:
– Calculated t-value
– Critical t-value
If calculated t-value is greater than critical t-
value, then reject the null hypothesis.
26. Computing the paired t-test
Pre post Difference
(D)
Difference 2
1 7 6 36
2 6 4 16
1 8 7 49
Sum=17 Sum = 101
29. Computing the paired t-test
Degree of freedom = n-1
= 2
Alpha = 0.05
Critical t from the t- table = 2.92
30. Computing the paired t-test
Calculated t > critical t
6.425 > 2.92
We will reject the null hypothesis. So, there is
a difference between pre and post
34. Paired test using SPSS
As the sig. < alpha
0.023 <0.05
Null hypothesis will be accepted
35. Summary
The t-test assesses whether the means of
two groups are statistically different from
each other
Independent t- test is to determine if a
difference exists in the means of two groups
on a particular characteristic.
Paired samples t-test is a measurements of
the same variable at two different points are
compared
36. Summary
To calculate t-test, we need two t-values:
– Calculated t-value
– Critical t-value
If calculated t-value is greater than critical t-
value, then reject the null hypothesis.
In SPSS:
– analyze for normal distribution
– Perform the test
– If the sig < alpha value, null hypothesis will be
accepted