This document provides information about the Kruskal-Wallis test, a non-parametric statistical method for comparing three or more groups of independent samples. It discusses the history of the test, its assumptions, how to calculate it, examples of its use, and references for further information. The Kruskal-Wallis test is used as an alternative to one-way ANOVA when assumptions of normality or equal variance are not met, to compare population medians among three or more groups. An example is provided to demonstrate how to perform the test on serum protein levels from three groups of patients.