SlideShare a Scribd company logo
Purification of Substances Highlight!! Sneak preview of our first session included!!
Have you wonder……. ,[object Object],[object Object],[object Object],Miscible mixtures
[object Object],[object Object],Today’s lesson Objectives: Know their principles, procedures and application
Chromatography ,[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],Paper Chromatography Aim:  To separate and identify mixtures of dyes.
Examples: dyes , food  colorings, forensics, Urine analysis etc. Paper Chromatography Solvent Solvent front Paper  Lid Resolved spots
Paper Chromatography – PROCEDURE 1 ,[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],Paper Chromatography – PROCEDURE 1
Paper Chromatography – PROCEDURE 1 At the Start At the End Chromatogram
[object Object],[object Object],[object Object],Paper Chromatography – PROCEDURE 1
Chromatography ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],Paper chromatography
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Paper chromatography
Paper Chromatography – PRINCIPLE 1 ,[object Object],[object Object],[object Object],Solubility in solvent
Chromatography ,[object Object],[object Object],[object Object]
Paper Chromatography – PRINCIPLE 1
Paper Chromatography – PRINCIPLE 2 ,[object Object],[object Object],[object Object],Mixed swarm of bees and hornets enter flower bed … Bees visit flowers; hornets don’t … Hornets leave the bed first …
THREE KINDS OF CHROMATOGRAMS THAT CAN BE DEVELOPED
Descending method  is better because ,[object Object],[object Object],Two types of Paper Chromatography
Paper Chromatography – Chromatogram ,[object Object],Orange Green Blue Red Black Unknown Known In the experiment, the  solvent  travels up the paper. The dyes on the pencil line  dissolve  in the solvent. The  more   soluble the dyes are in the solvent, the  faster  they move up the paper. So the dyes move up the paper at  different  speeds. Hence the dyes are separated. Colored Spot
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Paper Chromatography – Chromatogram
Which of the soft drinks contain a harmful dye? A)  P  and  Q     B)  R  and  S C)  Q  and  S   D)  P  and  R   P   Q   R   S   X   Y soft drinks  dyes Interpreting Chromatograms
What can be done if the substance is colourless ? ,[object Object],[object Object],[object Object],Chromatography
chromatography What happen if there are  more than 20  suspected substances in the sample? It would not be practical to put all the  suspected substances on the chromatogram. We can make use of the R f  values.
Paper Chromatography ,[object Object],It is easier and more sensible to  - measure the R f  value of each component  and then identify them by looking at known R f   in reference book. R f  value =   Distance travelled by the substance Distance travelled by the solvent
Chromatography A B B A Solvent used : alcohol
[object Object],[object Object],[object Object],[object Object],Chromatography
Applications: Chromatography Detection of  - drugs in athletes - pesticides sprayed on vegetables - illegal dyes in food
Paper Chromatography – APPLICATION ,[object Object],[object Object],[object Object],[object Object]
                                                                             Drugs have provided the world of athletics with a fierce opponent ever since the emergence of systematic forms of doping in eastern Europe.  But the most famous case in history is that involving a westerner - Canadian sprinter Ben Johnson. He won the 100 metres at the 1988 Seoul Olympics in a world record time but was later stripped of his gold medal and banned for two years after testing positive for an anabolic steroid.   Since then he has made an unsuccessful attempt to get back into the Canadian team for the 2000 Olympics.  There have also been some bizarre stunts such as a race with two horses and car for charity. The animals beat him although the car was stalled by wheelspin.  Johnson has recently worked as personal fitness trainer for footballer Said Gaddafi, son of Libyan president Colonel Muamar Gadaffi.  Meanwhile an incident in which a mugger took his wallet and outran him showed that the 38-year-old does not have the speed he once possessed.   Drugs in world athletics BBC Sport website (Mon 31 July 2000) Tainted win: Johnson (second left) wins in Seoul  A life ban from the track followed in March 1993 when he gave another positive test.
 
The Straits Times, 7 December 1988
 
Paper Chromatography – ADVANTAGES ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],What is a pure substance?
Effect of Impurities in mixtures ,[object Object],[object Object],[object Object],[object Object]
TESTING THE PURITY OF SUBSTANCES ,[object Object],[object Object],2. Boiling Point (Presence of impurities will  raise  the boiling point) 3. Chromatography (If impurities are present, there will be more    than one spots on the chromatogram) ,[object Object]
Chromatography ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],Paper chromatography 1 pencil line
[object Object],Paper chromatography 2 black red blue green orange drops of ink on the pencil line
[object Object],[object Object],[object Object],[object Object],Paper chromatography 3 cover
[object Object],[object Object],[object Object],[object Object],Paper chromatography How many components does black ink has? 4 chromatogram of dyes in different coloured inks black red blue green orange

More Related Content

What's hot

Paper & TLC Chromatography
Paper & TLC ChromatographyPaper & TLC Chromatography
Paper & TLC Chromatography
bvocmithilesh
 
Thin Layer Chromatography & Paper Chromatography
Thin Layer Chromatography & Paper ChromatographyThin Layer Chromatography & Paper Chromatography
Thin Layer Chromatography & Paper Chromatography
M.T.H Group
 
Separation of Mixtures
Separation of MixturesSeparation of Mixtures
Separation of Mixtures
Jacklyn Kong
 
Paper chromatography
Paper chromatographyPaper chromatography
Paper chromatographyshaisejacob
 
Thin layer chromatogaphy bpharm sud
Thin layer chromatogaphy bpharm sudThin layer chromatogaphy bpharm sud
Thin layer chromatogaphy bpharm sud
Dr. Sudheer Kumar Kamarapu
 
Thin layer chromatography (tlc)
Thin layer chromatography (tlc)Thin layer chromatography (tlc)
Thin layer chromatography (tlc)
Dr.M.Prasad Naidu
 
Kenyatta university biochem 1 st pract report.
Kenyatta university biochem 1 st pract report.Kenyatta university biochem 1 st pract report.
Kenyatta university biochem 1 st pract report.
Lando Elvis
 
Isolation and Purification of natural products by using chromatography, Dr.U....
Isolation and Purification of natural products by using chromatography, Dr.U....Isolation and Purification of natural products by using chromatography, Dr.U....
Isolation and Purification of natural products by using chromatography, Dr.U....
Srinivas college of pharmacy, Mangalore
 
Thin layer chromatography by khaleel S.G
Thin layer chromatography by khaleel S.GThin layer chromatography by khaleel S.G
Thin layer chromatography by khaleel S.G
Khaleel Basha
 
Chromatography
ChromatographyChromatography
Chromatography
muhammed2009
 
3122008 tlc
3122008 tlc3122008 tlc
3122008 tlc
rizka elvira
 
Paper chromatography bharm sud
Paper chromatography bharm sudPaper chromatography bharm sud
Paper chromatography bharm sud
Dr. Sudheer Kumar Kamarapu
 
Paper and thin layer chomatography by himaja
Paper and thin layer chomatography by himajaPaper and thin layer chomatography by himaja
Paper and thin layer chomatography by himaja
himaja donthula
 
Paper chromatography.pdf
Paper chromatography.pdfPaper chromatography.pdf
Paper chromatography.pdf
Deepaksahu1000
 
Thin Layer Chromatography
Thin Layer ChromatographyThin Layer Chromatography
Thin Layer Chromatography
Pranesh Kumar
 
TLC.....THIN LAYER CHROMATOGRAPHY..D.D.
TLC.....THIN LAYER CHROMATOGRAPHY..D.D.TLC.....THIN LAYER CHROMATOGRAPHY..D.D.
TLC.....THIN LAYER CHROMATOGRAPHY..D.D.
Deep Das
 
TLC Thin Layer Chromatography
TLC Thin Layer ChromatographyTLC Thin Layer Chromatography
TLC Thin Layer Chromatography
Hrishav Vardwaj
 
Thin layer-chromatography
Thin layer-chromatographyThin layer-chromatography
Thin layer-chromatography
Zainab&Sons
 
Chromatography
Chromatography Chromatography
Chromatography
Yahia Reda
 
Paper Chromatography | Types of Papers Used
Paper Chromatography | Types of Papers Used Paper Chromatography | Types of Papers Used
Paper Chromatography | Types of Papers Used
UBAID TARIQ
 

What's hot (20)

Paper & TLC Chromatography
Paper & TLC ChromatographyPaper & TLC Chromatography
Paper & TLC Chromatography
 
Thin Layer Chromatography & Paper Chromatography
Thin Layer Chromatography & Paper ChromatographyThin Layer Chromatography & Paper Chromatography
Thin Layer Chromatography & Paper Chromatography
 
Separation of Mixtures
Separation of MixturesSeparation of Mixtures
Separation of Mixtures
 
Paper chromatography
Paper chromatographyPaper chromatography
Paper chromatography
 
Thin layer chromatogaphy bpharm sud
Thin layer chromatogaphy bpharm sudThin layer chromatogaphy bpharm sud
Thin layer chromatogaphy bpharm sud
 
Thin layer chromatography (tlc)
Thin layer chromatography (tlc)Thin layer chromatography (tlc)
Thin layer chromatography (tlc)
 
Kenyatta university biochem 1 st pract report.
Kenyatta university biochem 1 st pract report.Kenyatta university biochem 1 st pract report.
Kenyatta university biochem 1 st pract report.
 
Isolation and Purification of natural products by using chromatography, Dr.U....
Isolation and Purification of natural products by using chromatography, Dr.U....Isolation and Purification of natural products by using chromatography, Dr.U....
Isolation and Purification of natural products by using chromatography, Dr.U....
 
Thin layer chromatography by khaleel S.G
Thin layer chromatography by khaleel S.GThin layer chromatography by khaleel S.G
Thin layer chromatography by khaleel S.G
 
Chromatography
ChromatographyChromatography
Chromatography
 
3122008 tlc
3122008 tlc3122008 tlc
3122008 tlc
 
Paper chromatography bharm sud
Paper chromatography bharm sudPaper chromatography bharm sud
Paper chromatography bharm sud
 
Paper and thin layer chomatography by himaja
Paper and thin layer chomatography by himajaPaper and thin layer chomatography by himaja
Paper and thin layer chomatography by himaja
 
Paper chromatography.pdf
Paper chromatography.pdfPaper chromatography.pdf
Paper chromatography.pdf
 
Thin Layer Chromatography
Thin Layer ChromatographyThin Layer Chromatography
Thin Layer Chromatography
 
TLC.....THIN LAYER CHROMATOGRAPHY..D.D.
TLC.....THIN LAYER CHROMATOGRAPHY..D.D.TLC.....THIN LAYER CHROMATOGRAPHY..D.D.
TLC.....THIN LAYER CHROMATOGRAPHY..D.D.
 
TLC Thin Layer Chromatography
TLC Thin Layer ChromatographyTLC Thin Layer Chromatography
TLC Thin Layer Chromatography
 
Thin layer-chromatography
Thin layer-chromatographyThin layer-chromatography
Thin layer-chromatography
 
Chromatography
Chromatography Chromatography
Chromatography
 
Paper Chromatography | Types of Papers Used
Paper Chromatography | Types of Papers Used Paper Chromatography | Types of Papers Used
Paper Chromatography | Types of Papers Used
 

Viewers also liked

CSI: Ink Chromatography & 3 mini labs
CSI: Ink Chromatography & 3 mini labsCSI: Ink Chromatography & 3 mini labs
CSI: Ink Chromatography & 3 mini labsMrs. Henley
 
Paper chromatography(final)
Paper chromatography(final)Paper chromatography(final)
Paper chromatography(final)Myquie de Guzman
 
Paper Chromatography PPT (new)
Paper Chromatography PPT (new)Paper Chromatography PPT (new)
Paper Chromatography PPT (new)shaisejacob
 
Purification Of Substances 1
Purification Of Substances 1Purification Of Substances 1
Purification Of Substances 1sYhira
 
Separation Techniques Revision PDF
Separation Techniques Revision PDFSeparation Techniques Revision PDF
Separation Techniques Revision PDFmissing island
 
chromatographic techniques
chromatographic techniqueschromatographic techniques
chromatographic techniques
Meenal Aggarwal
 
Paper Chromatography
Paper ChromatographyPaper Chromatography
Paper ChromatographyDavid Genis
 
Paper chromatography experiment
Paper chromatography experimentPaper chromatography experiment
Paper chromatography experiment
Allianze University
 
Principles and application of chromatography
Principles and application of chromatographyPrinciples and application of chromatography
Principles and application of chromatographysuniu
 
Separation methods
Separation methodsSeparation methods
Separation methods
Japheth Ofosu
 
CSI Chromatography
CSI ChromatographyCSI Chromatography
CSI Chromatography
Angela Rickard
 
Informe de lab organica #2
Informe de lab organica #2Informe de lab organica #2
Informe de lab organica #2Valeria Silva
 
CSI Handwriting Analysis
CSI Handwriting AnalysisCSI Handwriting Analysis
CSI Handwriting AnalysisMrs. Henley
 
Melting Point determination- Acetanilide, Benzoic Acid and Salicylic Acid
Melting Point determination- Acetanilide, Benzoic Acid and Salicylic AcidMelting Point determination- Acetanilide, Benzoic Acid and Salicylic Acid
Melting Point determination- Acetanilide, Benzoic Acid and Salicylic Acid
mariela sanota
 
Determination of boiling point of organic compounds
Determination of boiling point of organic compoundsDetermination of boiling point of organic compounds
Determination of boiling point of organic compoundsDotsha Raheem
 
Lecture 3 biochemical technique (2)
Lecture 3 biochemical technique (2)Lecture 3 biochemical technique (2)
Lecture 3 biochemical technique (2)
Sidra Shaffique
 
Organic chemistry: purification techniques
Organic chemistry: purification techniquesOrganic chemistry: purification techniques
Organic chemistry: purification techniquesAshima Aggarwal
 
Simple and fractional distillation
Simple and fractional distillationSimple and fractional distillation
Simple and fractional distillation
Mirza Salman Baig
 

Viewers also liked (20)

CSI: Ink Chromatography & 3 mini labs
CSI: Ink Chromatography & 3 mini labsCSI: Ink Chromatography & 3 mini labs
CSI: Ink Chromatography & 3 mini labs
 
Paper chromatography(final)
Paper chromatography(final)Paper chromatography(final)
Paper chromatography(final)
 
Paper Chromatography PPT (new)
Paper Chromatography PPT (new)Paper Chromatography PPT (new)
Paper Chromatography PPT (new)
 
Purification Of Substances 1
Purification Of Substances 1Purification Of Substances 1
Purification Of Substances 1
 
Separation Techniques Revision PDF
Separation Techniques Revision PDFSeparation Techniques Revision PDF
Separation Techniques Revision PDF
 
chromatographic techniques
chromatographic techniqueschromatographic techniques
chromatographic techniques
 
Paper Chromatography
Paper ChromatographyPaper Chromatography
Paper Chromatography
 
Paper chromatography experiment
Paper chromatography experimentPaper chromatography experiment
Paper chromatography experiment
 
Principles and application of chromatography
Principles and application of chromatographyPrinciples and application of chromatography
Principles and application of chromatography
 
Separation methods
Separation methodsSeparation methods
Separation methods
 
CSI Chromatography
CSI ChromatographyCSI Chromatography
CSI Chromatography
 
Informe de lab organica #2
Informe de lab organica #2Informe de lab organica #2
Informe de lab organica #2
 
CSI Handwriting Analysis
CSI Handwriting AnalysisCSI Handwriting Analysis
CSI Handwriting Analysis
 
Melting Point determination- Acetanilide, Benzoic Acid and Salicylic Acid
Melting Point determination- Acetanilide, Benzoic Acid and Salicylic AcidMelting Point determination- Acetanilide, Benzoic Acid and Salicylic Acid
Melting Point determination- Acetanilide, Benzoic Acid and Salicylic Acid
 
Determination of boiling point of organic compounds
Determination of boiling point of organic compoundsDetermination of boiling point of organic compounds
Determination of boiling point of organic compounds
 
Lecture 3 biochemical technique (2)
Lecture 3 biochemical technique (2)Lecture 3 biochemical technique (2)
Lecture 3 biochemical technique (2)
 
Organic chemistry: purification techniques
Organic chemistry: purification techniquesOrganic chemistry: purification techniques
Organic chemistry: purification techniques
 
Simple and fractional distillation
Simple and fractional distillationSimple and fractional distillation
Simple and fractional distillation
 
Lab4 chromatography
Lab4 chromatographyLab4 chromatography
Lab4 chromatography
 
TLC
TLCTLC
TLC
 

Similar to Purification Of Substances 2

Chromatogaphy powerpoint
Chromatogaphy powerpointChromatogaphy powerpoint
Chromatogaphy powerpointAmyDeBell
 
Thin-Layer-Chromatography-of-inkdyes.pdf
Thin-Layer-Chromatography-of-inkdyes.pdfThin-Layer-Chromatography-of-inkdyes.pdf
Thin-Layer-Chromatography-of-inkdyes.pdf
m65209157
 
Paper Chromatography.pptx
Paper Chromatography.pptxPaper Chromatography.pptx
Paper Chromatography.pptx
sanjayudps2016
 
CHROMOTOGRAPHY.ppt
CHROMOTOGRAPHY.pptCHROMOTOGRAPHY.ppt
CHROMOTOGRAPHY.ppt
BobSojourner
 
chemistry project on blah blah bahl hsld
chemistry project on blah blah bahl hsldchemistry project on blah blah bahl hsld
chemistry project on blah blah bahl hsld
yogitakatiyar81
 
Chromanotes09
Chromanotes09Chromanotes09
Chromanotes09
Timofiev
 
chromanotes09.ppt
chromanotes09.pptchromanotes09.ppt
chromanotes09.ppt
kaushalkumar1253
 
Paper chromatography
Paper chromatographyPaper chromatography
Paper chromatography
Dr.M.Prasad Naidu
 
Chromatography
ChromatographyChromatography
Chromatography
dharti bandarwar
 
Paper chromatography
Paper chromatographyPaper chromatography
Paper chromatography
NITESH KUMAR
 
Paper chromatography
Paper chromatographyPaper chromatography
Paper chromatography
Nilesh Thakare
 
Chromatography presentation
Chromatography presentationChromatography presentation
Chromatography presentation
Shamima Nasrin Bithi
 
Paper chromatography
Paper chromatographyPaper chromatography
Paper chromatography
Madhusudan Bachute
 
Chromatography by narayan sarkar and simi baruah
Chromatography by  narayan sarkar and simi baruahChromatography by  narayan sarkar and simi baruah
Chromatography by narayan sarkar and simi baruah
NarayanSarkar6
 
Paper chromatography
Paper chromatographyPaper chromatography
Paper chromatography
PragyaSharma230
 
Paper chromatography
Paper chromatographyPaper chromatography
Paper chromatography
Hafiz M Waseem
 
Chromatography.pdf
Chromatography.pdfChromatography.pdf
Chromatography.pdf
Prawesh1
 

Similar to Purification Of Substances 2 (20)

Chrmo
ChrmoChrmo
Chrmo
 
Chromatogaphy powerpoint
Chromatogaphy powerpointChromatogaphy powerpoint
Chromatogaphy powerpoint
 
Thin-Layer-Chromatography-of-inkdyes.pdf
Thin-Layer-Chromatography-of-inkdyes.pdfThin-Layer-Chromatography-of-inkdyes.pdf
Thin-Layer-Chromatography-of-inkdyes.pdf
 
Paper Chromatography.pptx
Paper Chromatography.pptxPaper Chromatography.pptx
Paper Chromatography.pptx
 
Chromatography1
Chromatography1Chromatography1
Chromatography1
 
CHROMOTOGRAPHY.ppt
CHROMOTOGRAPHY.pptCHROMOTOGRAPHY.ppt
CHROMOTOGRAPHY.ppt
 
chemistry project on blah blah bahl hsld
chemistry project on blah blah bahl hsldchemistry project on blah blah bahl hsld
chemistry project on blah blah bahl hsld
 
Chromanotes09
Chromanotes09Chromanotes09
Chromanotes09
 
chromanotes09.ppt
chromanotes09.pptchromanotes09.ppt
chromanotes09.ppt
 
Paper chromatography
Paper chromatographyPaper chromatography
Paper chromatography
 
Chromanotes09
Chromanotes09Chromanotes09
Chromanotes09
 
Chromatography
ChromatographyChromatography
Chromatography
 
Paper chromatography
Paper chromatographyPaper chromatography
Paper chromatography
 
Paper chromatography
Paper chromatographyPaper chromatography
Paper chromatography
 
Chromatography presentation
Chromatography presentationChromatography presentation
Chromatography presentation
 
Paper chromatography
Paper chromatographyPaper chromatography
Paper chromatography
 
Chromatography by narayan sarkar and simi baruah
Chromatography by  narayan sarkar and simi baruahChromatography by  narayan sarkar and simi baruah
Chromatography by narayan sarkar and simi baruah
 
Paper chromatography
Paper chromatographyPaper chromatography
Paper chromatography
 
Paper chromatography
Paper chromatographyPaper chromatography
Paper chromatography
 
Chromatography.pdf
Chromatography.pdfChromatography.pdf
Chromatography.pdf
 

Recently uploaded

How to Break the cycle of negative Thoughts
How to Break the cycle of negative ThoughtsHow to Break the cycle of negative Thoughts
How to Break the cycle of negative Thoughts
Col Mukteshwar Prasad
 
Ethnobotany and Ethnopharmacology ......
Ethnobotany and Ethnopharmacology ......Ethnobotany and Ethnopharmacology ......
Ethnobotany and Ethnopharmacology ......
Ashokrao Mane college of Pharmacy Peth-Vadgaon
 
Students, digital devices and success - Andreas Schleicher - 27 May 2024..pptx
Students, digital devices and success - Andreas Schleicher - 27 May 2024..pptxStudents, digital devices and success - Andreas Schleicher - 27 May 2024..pptx
Students, digital devices and success - Andreas Schleicher - 27 May 2024..pptx
EduSkills OECD
 
Model Attribute Check Company Auto Property
Model Attribute  Check Company Auto PropertyModel Attribute  Check Company Auto Property
Model Attribute Check Company Auto Property
Celine George
 
Thesis Statement for students diagnonsed withADHD.ppt
Thesis Statement for students diagnonsed withADHD.pptThesis Statement for students diagnonsed withADHD.ppt
Thesis Statement for students diagnonsed withADHD.ppt
EverAndrsGuerraGuerr
 
MARUTI SUZUKI- A Successful Joint Venture in India.pptx
MARUTI SUZUKI- A Successful Joint Venture in India.pptxMARUTI SUZUKI- A Successful Joint Venture in India.pptx
MARUTI SUZUKI- A Successful Joint Venture in India.pptx
bennyroshan06
 
How to Make a Field invisible in Odoo 17
How to Make a Field invisible in Odoo 17How to Make a Field invisible in Odoo 17
How to Make a Field invisible in Odoo 17
Celine George
 
2024.06.01 Introducing a competency framework for languag learning materials ...
2024.06.01 Introducing a competency framework for languag learning materials ...2024.06.01 Introducing a competency framework for languag learning materials ...
2024.06.01 Introducing a competency framework for languag learning materials ...
Sandy Millin
 
Palestine last event orientationfvgnh .pptx
Palestine last event orientationfvgnh .pptxPalestine last event orientationfvgnh .pptx
Palestine last event orientationfvgnh .pptx
RaedMohamed3
 
Synthetic Fiber Construction in lab .pptx
Synthetic Fiber Construction in lab .pptxSynthetic Fiber Construction in lab .pptx
Synthetic Fiber Construction in lab .pptx
Pavel ( NSTU)
 
Operation Blue Star - Saka Neela Tara
Operation Blue Star   -  Saka Neela TaraOperation Blue Star   -  Saka Neela Tara
Operation Blue Star - Saka Neela Tara
Balvir Singh
 
How to Split Bills in the Odoo 17 POS Module
How to Split Bills in the Odoo 17 POS ModuleHow to Split Bills in the Odoo 17 POS Module
How to Split Bills in the Odoo 17 POS Module
Celine George
 
Introduction to Quality Improvement Essentials
Introduction to Quality Improvement EssentialsIntroduction to Quality Improvement Essentials
Introduction to Quality Improvement Essentials
Excellence Foundation for South Sudan
 
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
siemaillard
 
Language Across the Curriculm LAC B.Ed.
Language Across the  Curriculm LAC B.Ed.Language Across the  Curriculm LAC B.Ed.
Language Across the Curriculm LAC B.Ed.
Atul Kumar Singh
 
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdf
Welcome to TechSoup   New Member Orientation and Q&A (May 2024).pdfWelcome to TechSoup   New Member Orientation and Q&A (May 2024).pdf
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdf
TechSoup
 
The French Revolution Class 9 Study Material pdf free download
The French Revolution Class 9 Study Material pdf free downloadThe French Revolution Class 9 Study Material pdf free download
The French Revolution Class 9 Study Material pdf free download
Vivekanand Anglo Vedic Academy
 
The geography of Taylor Swift - some ideas
The geography of Taylor Swift - some ideasThe geography of Taylor Swift - some ideas
The geography of Taylor Swift - some ideas
GeoBlogs
 
The Roman Empire A Historical Colossus.pdf
The Roman Empire A Historical Colossus.pdfThe Roman Empire A Historical Colossus.pdf
The Roman Empire A Historical Colossus.pdf
kaushalkr1407
 
Mule 4.6 & Java 17 Upgrade | MuleSoft Mysore Meetup #46
Mule 4.6 & Java 17 Upgrade | MuleSoft Mysore Meetup #46Mule 4.6 & Java 17 Upgrade | MuleSoft Mysore Meetup #46
Mule 4.6 & Java 17 Upgrade | MuleSoft Mysore Meetup #46
MysoreMuleSoftMeetup
 

Recently uploaded (20)

How to Break the cycle of negative Thoughts
How to Break the cycle of negative ThoughtsHow to Break the cycle of negative Thoughts
How to Break the cycle of negative Thoughts
 
Ethnobotany and Ethnopharmacology ......
Ethnobotany and Ethnopharmacology ......Ethnobotany and Ethnopharmacology ......
Ethnobotany and Ethnopharmacology ......
 
Students, digital devices and success - Andreas Schleicher - 27 May 2024..pptx
Students, digital devices and success - Andreas Schleicher - 27 May 2024..pptxStudents, digital devices and success - Andreas Schleicher - 27 May 2024..pptx
Students, digital devices and success - Andreas Schleicher - 27 May 2024..pptx
 
Model Attribute Check Company Auto Property
Model Attribute  Check Company Auto PropertyModel Attribute  Check Company Auto Property
Model Attribute Check Company Auto Property
 
Thesis Statement for students diagnonsed withADHD.ppt
Thesis Statement for students diagnonsed withADHD.pptThesis Statement for students diagnonsed withADHD.ppt
Thesis Statement for students diagnonsed withADHD.ppt
 
MARUTI SUZUKI- A Successful Joint Venture in India.pptx
MARUTI SUZUKI- A Successful Joint Venture in India.pptxMARUTI SUZUKI- A Successful Joint Venture in India.pptx
MARUTI SUZUKI- A Successful Joint Venture in India.pptx
 
How to Make a Field invisible in Odoo 17
How to Make a Field invisible in Odoo 17How to Make a Field invisible in Odoo 17
How to Make a Field invisible in Odoo 17
 
2024.06.01 Introducing a competency framework for languag learning materials ...
2024.06.01 Introducing a competency framework for languag learning materials ...2024.06.01 Introducing a competency framework for languag learning materials ...
2024.06.01 Introducing a competency framework for languag learning materials ...
 
Palestine last event orientationfvgnh .pptx
Palestine last event orientationfvgnh .pptxPalestine last event orientationfvgnh .pptx
Palestine last event orientationfvgnh .pptx
 
Synthetic Fiber Construction in lab .pptx
Synthetic Fiber Construction in lab .pptxSynthetic Fiber Construction in lab .pptx
Synthetic Fiber Construction in lab .pptx
 
Operation Blue Star - Saka Neela Tara
Operation Blue Star   -  Saka Neela TaraOperation Blue Star   -  Saka Neela Tara
Operation Blue Star - Saka Neela Tara
 
How to Split Bills in the Odoo 17 POS Module
How to Split Bills in the Odoo 17 POS ModuleHow to Split Bills in the Odoo 17 POS Module
How to Split Bills in the Odoo 17 POS Module
 
Introduction to Quality Improvement Essentials
Introduction to Quality Improvement EssentialsIntroduction to Quality Improvement Essentials
Introduction to Quality Improvement Essentials
 
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
 
Language Across the Curriculm LAC B.Ed.
Language Across the  Curriculm LAC B.Ed.Language Across the  Curriculm LAC B.Ed.
Language Across the Curriculm LAC B.Ed.
 
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdf
Welcome to TechSoup   New Member Orientation and Q&A (May 2024).pdfWelcome to TechSoup   New Member Orientation and Q&A (May 2024).pdf
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdf
 
The French Revolution Class 9 Study Material pdf free download
The French Revolution Class 9 Study Material pdf free downloadThe French Revolution Class 9 Study Material pdf free download
The French Revolution Class 9 Study Material pdf free download
 
The geography of Taylor Swift - some ideas
The geography of Taylor Swift - some ideasThe geography of Taylor Swift - some ideas
The geography of Taylor Swift - some ideas
 
The Roman Empire A Historical Colossus.pdf
The Roman Empire A Historical Colossus.pdfThe Roman Empire A Historical Colossus.pdf
The Roman Empire A Historical Colossus.pdf
 
Mule 4.6 & Java 17 Upgrade | MuleSoft Mysore Meetup #46
Mule 4.6 & Java 17 Upgrade | MuleSoft Mysore Meetup #46Mule 4.6 & Java 17 Upgrade | MuleSoft Mysore Meetup #46
Mule 4.6 & Java 17 Upgrade | MuleSoft Mysore Meetup #46
 

Purification Of Substances 2

  • 1. Purification of Substances Highlight!! Sneak preview of our first session included!!
  • 2.
  • 3.
  • 4.
  • 5.
  • 6. Examples: dyes , food colorings, forensics, Urine analysis etc. Paper Chromatography Solvent Solvent front Paper Lid Resolved spots
  • 7.
  • 8.
  • 9. Paper Chromatography – PROCEDURE 1 At the Start At the End Chromatogram
  • 10.
  • 11.
  • 12.
  • 13.
  • 14.
  • 15.
  • 17.
  • 18. THREE KINDS OF CHROMATOGRAMS THAT CAN BE DEVELOPED
  • 19.
  • 20.
  • 21.
  • 22. Which of the soft drinks contain a harmful dye? A) P and Q B) R and S C) Q and S D) P and R P Q R S X Y soft drinks dyes Interpreting Chromatograms
  • 23.
  • 24. chromatography What happen if there are more than 20 suspected substances in the sample? It would not be practical to put all the suspected substances on the chromatogram. We can make use of the R f values.
  • 25.
  • 26. Chromatography A B B A Solvent used : alcohol
  • 27.
  • 28. Applications: Chromatography Detection of - drugs in athletes - pesticides sprayed on vegetables - illegal dyes in food
  • 29.
  • 30.                                                                             Drugs have provided the world of athletics with a fierce opponent ever since the emergence of systematic forms of doping in eastern Europe. But the most famous case in history is that involving a westerner - Canadian sprinter Ben Johnson. He won the 100 metres at the 1988 Seoul Olympics in a world record time but was later stripped of his gold medal and banned for two years after testing positive for an anabolic steroid. Since then he has made an unsuccessful attempt to get back into the Canadian team for the 2000 Olympics. There have also been some bizarre stunts such as a race with two horses and car for charity. The animals beat him although the car was stalled by wheelspin. Johnson has recently worked as personal fitness trainer for footballer Said Gaddafi, son of Libyan president Colonel Muamar Gadaffi. Meanwhile an incident in which a mugger took his wallet and outran him showed that the 38-year-old does not have the speed he once possessed. Drugs in world athletics BBC Sport website (Mon 31 July 2000) Tainted win: Johnson (second left) wins in Seoul A life ban from the track followed in March 1993 when he gave another positive test.
  • 31.  
  • 32. The Straits Times, 7 December 1988
  • 33.  
  • 34.
  • 35.
  • 36.
  • 37.
  • 38.
  • 39.
  • 40.
  • 41.
  • 42.