Prokaryotes are organisms that consist of a single prokaryotic cell. Eukaryotic cells are found in plants, animals, fungi, and protists. They range from 10–100 μm in diameter, and their DNA is contained within a membrane-bound nucleus.Prokaryotes do not have membrane-enclosed nuclei. Therefore, the processes of transcription, translation, and mRNA degradation can all occur simultaneously.
An Overview...
Definition of Translation.
Def. of Eukaryotes.
Translation: An Overview.
Components of Translation.
Some Enzymes .
Ribosome Role.
Mechanism of Translation.
Initiation.
Scanning Model of Initiation.
Initiation Factors.
Animation.
Elongation.
Chain Elongation: Translocation.
Animation.
Termination.
Animation....
It's not perfect still... what are your views friends?
The base sequence information present in the gene (DNA) is copied into an RNA molecule, which directly participates in protein synthesis and provides information for amino acid sequence of the protein. This RNA molecule is called messenger RNA or mRNA. The process of production of RNA copy of a DNA sequence is called transcription; this reaction is catalyzed by DNA-directed RNA polymerase, or simply RNA polymerase.
An Overview...
Definition of Translation.
Def. of Eukaryotes.
Translation: An Overview.
Components of Translation.
Some Enzymes .
Ribosome Role.
Mechanism of Translation.
Initiation.
Scanning Model of Initiation.
Initiation Factors.
Animation.
Elongation.
Chain Elongation: Translocation.
Animation.
Termination.
Animation....
It's not perfect still... what are your views friends?
The base sequence information present in the gene (DNA) is copied into an RNA molecule, which directly participates in protein synthesis and provides information for amino acid sequence of the protein. This RNA molecule is called messenger RNA or mRNA. The process of production of RNA copy of a DNA sequence is called transcription; this reaction is catalyzed by DNA-directed RNA polymerase, or simply RNA polymerase.
it describes transcription with simple diagram and animation. its steps and inhibitors are described for both eukaryotes and prokaryotes. it will be easily understood by UG students . post transcriptional modification of all the RNA are also described with diagrams.
1.Definition
2.Transcription is selective
3.Transcription in Prokaryotes
•Initiation
•Elongation
•RNA polymerase vs DNA polymerase
•Termination
4.Transcription in Eukaryotes
•Initiation
•Elongation
•Termination
•Post transcriptional modifications
RNA Polymerase
Introduction
Purification
History
PRODUCTS OF RNAP
Messenger RNA
Non-coding RNA or "RNA genes
Transfer RNA
Ribosomal RNA
Micro RNA
Catalytic RNA (Ribozyme)
prokaryotic and eukaryotic
Transcription by RNA Polymerase
TYPES OF RNA POLYMERASE
Type I
Type II
Type III
Prokaryotic Transcription Unit
EXPRESSION OF A PROKARYOTIC GENE
Prokaryotic Polycistronic Message Codes for Several Different Proteins
Eukaryotic Transcription Unit
ENHANCERS AND SILENCERS
RESULT OF THE TRANSCRIPTION CYCLE
RNAP III TRANSCRIBES HUMAN MICRORNAS
RNAP I–specific subunits promotepolymerase clustering to enhance the rRNA genetranscription cycle
RNAP II–TFIIB STRUCTURE ANDMECHANISM OF TRANSCRIPTION INITIATION
FIVE CHECKPOINTS MAINTAINING THE FIDELITY OFTRANSCRIPTION BY RNAP IN STRUCTURAL ANDENERGETIC DETAILS
it describes transcription with simple diagram and animation. its steps and inhibitors are described for both eukaryotes and prokaryotes. it will be easily understood by UG students . post transcriptional modification of all the RNA are also described with diagrams.
1.Definition
2.Transcription is selective
3.Transcription in Prokaryotes
•Initiation
•Elongation
•RNA polymerase vs DNA polymerase
•Termination
4.Transcription in Eukaryotes
•Initiation
•Elongation
•Termination
•Post transcriptional modifications
RNA Polymerase
Introduction
Purification
History
PRODUCTS OF RNAP
Messenger RNA
Non-coding RNA or "RNA genes
Transfer RNA
Ribosomal RNA
Micro RNA
Catalytic RNA (Ribozyme)
prokaryotic and eukaryotic
Transcription by RNA Polymerase
TYPES OF RNA POLYMERASE
Type I
Type II
Type III
Prokaryotic Transcription Unit
EXPRESSION OF A PROKARYOTIC GENE
Prokaryotic Polycistronic Message Codes for Several Different Proteins
Eukaryotic Transcription Unit
ENHANCERS AND SILENCERS
RESULT OF THE TRANSCRIPTION CYCLE
RNAP III TRANSCRIBES HUMAN MICRORNAS
RNAP I–specific subunits promotepolymerase clustering to enhance the rRNA genetranscription cycle
RNAP II–TFIIB STRUCTURE ANDMECHANISM OF TRANSCRIPTION INITIATION
FIVE CHECKPOINTS MAINTAINING THE FIDELITY OFTRANSCRIPTION BY RNAP IN STRUCTURAL ANDENERGETIC DETAILS
Photosystem II captures and transfers energy.
– chlorophyll absorbs
energy from sunlight
– energized electrons
enter electron
transport chain
– water molecules are
split
– oxygen is released as
waste
– hydrogen ions are
transported across
thylakoid membrane
4.1 Chemical Energy and ATP
• Photosystem I captures energy and produces energycarrying molecules.
– chlorophyll absorbs
energy from sunlight
– energized electrons
are used to make
NADPH
– NADPH is transferred
to light-independent
reactions
4.1 Chemical Energy and ATP
• The light-dependent reactions produce ATP.
– hydrogen ions flow through a channel in the thylakoid
membrane
– ATP synthase attached to the channel makes ATP
4.1 Chemical Energy and ATP
• Light-independent
reactions occur in the
stroma and use CO2
molecules.
The second stage of photosynthesis uses energy from
the first stage to make sugars.
4.1 Chemical Energy and ATP
• A molecule of glucose is formed as it stores some of the
energy captured from sunlight.
– carbon dioxide molecules enter the Calvin Photosystem II captures and transfers energy.
– chlorophyll absorbs
energy from sunlight
– energized electrons
enter electron
transport chain
– water molecules are
split
– oxygen is released as
waste
– hydrogen ions are
transported across
thylakoid membrane
4.1 Chemical Energy and ATP
• Photosystem I captures energy and produces energycarrying molecules.
– chlorophyll absorbs
energy from sunlight
– energized electrons
are used to make
NADPH
– NADPH is transferred
to light-independent
reactions
4.1 Chemical Energy and ATP
• The light-dependent reactions produce ATP.
– hydrogen ions flow through a channel in the thylakoid
membrane
– ATP synthase attached to the channel makes ATP
4.1 Chemical Energy and ATP
• Light-independent
reactions occur in the
stroma and use CO2
molecules.
The second stage of photosynthesis uses energy from
the first stage to make sugars.
4.1 Chemical Energy and ATP
• A molecule of glucose is formed as it stores some of the
energy captured from sunlight.
– carbon dioxide molecules enter the Calvin Photosystem II captures and transfers energy.
– chlorophyll absorbs
energy from sunlight
– energized electrons
enter electron
transport chain
– water molecules are
split
– oxygen is released as
waste
– hydrogen ions are
transported across
thylakoid membrane
4.1 Chemical Energy and ATP
• Photosystem I captures energy and produces energycarrying molecules.
– chlorophyll absorbs
energy from sunlight
– energized electrons
are used to make
NADPH
– NADPH is transferred
to light-independent
reactions
4.1 Chemical Energy and ATP
• The light-dependent reactions produce ATP.
– hydrogen ions flow through a channel in the thylakoid
membrane
– ATP synthase attached to the channel makes ATP
4.1 Chemical Energy and ATP
• Light-independent
reactions occur in the
stroma and use CO2
molecules.
The second stage of photosynthesis uses energy frvf
description of mechanism of transcription in prokaryotes and eukaryotes with clear explanation and clear pictures and also mentiong of different promotors and enhancers and silencers
The flow of information in the cell starts at DNA, which replicates to form more DNA. Information is then ‘transcribed” into RNA, and then it is “translated” into protein.
Information does not flow in the other direction.
A few exceptions to the Central Dogma exist
some RNA viruses, called “retroviruses”.
dna transcription is a important topic for biology student. this presentation may be helpful for student of biology.it is useful for all types of courses as like M.Sc, B.Sc, 11th and 12th standard.
This presentation explains DNA transcription and RNA Processing.
It gives details about prokaryotic DNA transcription and eukaryotic DNA transcription. it also explains post-transcriptional modification both in prokaryotes and eukaryotes.
Bioleaching, or microbial ore leaching, is a process used to extract metals from their ores using bacterial micro-organisms.
The bacteria feed on nutrients in the minerals, causing the metal to separate from its ore.
Biodiesel is a renewable, biodegradable fuel manufactured domestically from vegetable oils, animal fats, or recycled restaurant grease. ... Biodiesel is a liquid fuel often referred to as B100 or neat biodiesel in its pure, unblended form. Like petroleum diesel, biodiesel is used to fuel compression-ignition engines.
Rhabdoviridae is a family of negative-strand RNA viruses in the order Mononegavirales. Vertebrates, invertebrates, and plants serve as natural hosts. Diseases associated with member viruses include rabies encephalitis caused by the rabies virus, and flu-like symptoms in humans caused by vesiculoviruses.
The heap technique for biological soil treatment is an ex situ technology, that is, the contaminated soil is excavated and separated from the uncontaminated material.
Methanogenesis or biomethanation is the formation of methane by microbes known as methanogens. Organisms capable of producing methane have been identified only from the domain Archaea, a group phylogenetically distinct from both eukaryotes and bacteria, although many live in close association with anaerobic bacteria.
Biogas is an environmentally-friendly, renewable energy source. It's produced when organic matter, such as food or animal waste, is broken down by microorganisms in the absence of oxygen, in a process called anaerobic digestion.
Biomedical waste or hospital waste is any kind of waste containing infectious (or potentially infectious) materials. ... Waste sharps include potentially contaminated used (and unused discarded) needles, scalpels, lancets and other devices capable of penetrating skin.
Phytoremediation is defined as the use of higher plants for the cost-effective, environmentally friendly rehabilitation of soil and groundwater contaminated by toxic metals and organic compounds.
It is the process of synthesis of protein by encoding information on mRNA.
Protein synthesis requires mRNA, tRNA, aminoacids, ribosome and enzyme aminoacyl tRNA synthase
A trademark is a sign capable of distinguishing the goods or services of one enterprise from those of other enterprises. Trademarks are protected by intellectual property rights.
A trademark is a sign capable of distinguishing the goods or services of one enterprise from those of other enterprises. Trademarks are protected by intellectual property rights.
A trademark is a sign capable of distinguishing the goods or services of one enterprise from those of other enterprises. Trademarks are protected by intellectual property rights.
A trademark is a sign capable of distinguishing the goods or services of one enterprise from those of other enterprises. Trademarks are protected by intellectual property rights
The word Algorithm means “a process or set of rules to be followed in calculations or other problem-solving operations”.
flowchart is a type of diagram that represents an algorithm, workflow or process.
Ouchterlony double immunodiffusion (also known as passive double immunodiffusion) is an immunological technique used in the detection, identification and quantification of antibodies and antigens, such as immunoglobulins and extractable nuclear antigens.
A RIA is a very sensitive in vitro assay technique used to measure concentrations of substances, usually measuring antigen concentrations (for example, hormone levels in blood) by use of antibodies.
Eukaryotic transcription is the elaborate process that eukaryotic cells use to copy genetic information stored in DNA into units of transportable complementary RNA replica.
Post-transcriptional modification or co-transcriptional modification is a set of biological processes common to most eukaryotic cells by which an RNA primary transcript is chemically altered following transcription from a gene to produce a mature, functional RNA molecule
Transcription is the process by which the information in a strand of DNA is copied into a new molecule of messenger RNA (mRNA).Transcription is carried out by an enzyme called RNA polymerase and a number of accessory proteins called transcription factors.
Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
Synthetic fiber production is a fascinating and complex field that blends chemistry, engineering, and environmental science. By understanding these aspects, students can gain a comprehensive view of synthetic fiber production, its impact on society and the environment, and the potential for future innovations. Synthetic fibers play a crucial role in modern society, impacting various aspects of daily life, industry, and the environment. ynthetic fibers are integral to modern life, offering a range of benefits from cost-effectiveness and versatility to innovative applications and performance characteristics. While they pose environmental challenges, ongoing research and development aim to create more sustainable and eco-friendly alternatives. Understanding the importance of synthetic fibers helps in appreciating their role in the economy, industry, and daily life, while also emphasizing the need for sustainable practices and innovation.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
The Indian economy is classified into different sectors to simplify the analysis and understanding of economic activities. For Class 10, it's essential to grasp the sectors of the Indian economy, understand their characteristics, and recognize their importance. This guide will provide detailed notes on the Sectors of the Indian Economy Class 10, using specific long-tail keywords to enhance comprehension.
For more information, visit-www.vavaclasses.com
We all have good and bad thoughts from time to time and situation to situation. We are bombarded daily with spiraling thoughts(both negative and positive) creating all-consuming feel , making us difficult to manage with associated suffering. Good thoughts are like our Mob Signal (Positive thought) amidst noise(negative thought) in the atmosphere. Negative thoughts like noise outweigh positive thoughts. These thoughts often create unwanted confusion, trouble, stress and frustration in our mind as well as chaos in our physical world. Negative thoughts are also known as “distorted thinking”.
How to Create Map Views in the Odoo 17 ERPCeline George
The map views are useful for providing a geographical representation of data. They allow users to visualize and analyze the data in a more intuitive manner.
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
5. TRANSCRIPTION INITIATION
• A particular DNA sequence called as promoter is required in
prokaryotes to find the right starting point of RNA polymerase enzyme
transcription .
• This section is located close to the start of transcription.
• Promoters are important part of the regulatory region of a gene.
• The synthesis of RNA transcript starts 5’ of the promoter and
continues towards 3’.
• Being in 5 prime of the gene, promoter region is also referred to
regulator 5 .
6. Process : facilitated diffusion mediated by nonspecific binding.
It was proposed that sequences in the −35 region would affect
the initial binding of RNA polymerase to the promoter
7. How does the enzyme move from a random
binding site on DNA to a promoter?
• There is considerable evidence that at least three different processes
contribute to the rate of promoter search by RNA polymerase .
• First, the enzyme may move in a one-dimensional random walk along
the DNA ("sliding" ) .
• Second, given the intricately folded nature of the chromosome in the
bacterial nucleoid, having bound to one sequence on the chromosome,
the enzyme is now closer to other sites, reducing the time needed for
dissociation and rebinding to another site ("intersegment transfer" or
"hopping").
• Third, while bound nonspecifically to one site, the enzyme may
exchange DNA sites until a promoter is found ("direct transfer" ).
8. PROKARYOTIC PROMOTERS
• The promoters of prokaryotes are comprised of two short sequences
from the transcriptional start site which are located upstream at -10
and -35 bp (base pairs) position.
• Sequence at position -10 is called Pribnow box or the element of -10.
This sequence usually contains 6 nucleotide as TATAAT.
• Pribnow box is required for the initiation of transcription in
prokaryotes.
• The other sequence at position -35, is mostly composed of six
nucleotide comprising TTGACA.
• The presence of this -35 sequence position ensures the higher rate of
transcription
9. • Binding of RNA-polymerase (RNAP) to the promoter region is
followed by a conformational change of the RNAP, and the first
nucleotide (almost always a purine) then associates with the initiation
site on the subunit of the enzyme.
• In the presence of the appropriate nucleotide, RNAP catalyzes the
formation of a phosphodiester bond, and the nascent chain is now
attached to the polymerization site on the subunit of RNAP.
• In both prokaryotes and eukaryotes, a purine ribonucleotide is usually
the first to be polymerized into the RNA molecule.
• After 10–20 nucleotides have been polymerized, RNAP undergoes a
second conformational change leading to promoter clearance.
• Once this transition occurs, RNAP physically moves away from the
promoter, transcribing down the transcription unit, leading to the next
phase of the process, elongation.
10. Not all RNA polymerase complexes transcribe until the end of the
gene. Many transcription complexes dissociate from the template
after adding a couple of rNTPs, a process called abortive
transcription.
11. STEPS OF INITIATION
• Sigma factor (σ) binds to promoter sequence (-10, -35 sequence).
• Core enzyme binds to the sigma factor (σ) and promoter but DNA is
still closed.
• This is called the closed promoter complex.
• Holoenzyme untwist the double strands of DNA.
• Untwisted promoter is called the open promoter complex.
• RNA polymerase binds to -10 sequence and placed in position to start
transcribing.
• Sigma factor (σ) is released so that core enzyme can go forward
transcribing.
13. A ternary complex composed of RNA polymerase (RNAP), DNA template,
and RNA transcript is the central intermediate in the transcription cycle
responsible for the elongation of the RNA chain.
14. • RNA polymerase (core enzyme) moves along to transcribe the DNA
sequence into a single strand RNA of the coding gene.
• When transcribing, the RNA polymerase interact with DNA sequence
forming transcription bubble.
• DNA double helix is reformed as the RNA polymerase moves
forward.
• Few RNA nucleotides (newly synthesized) form an RNA/DNA hybrid
within RNA polymerase.
• As transcription proceed, single strand RNA gets out of the RNA
polymerase.
TRANSCRIPTION ELONGATION
15. A transcription bubble is a molecular structure formed during DNA transcription when
a limited portion of the DNA double strand is unwound. The size of a transcription
bubble ranges from 12-14 base pairs. A transcription bubble is formed when the RNA
polymerase enzyme binds to a promoter and causes two DNA strands to detach. It
presents a region of unpaired DNA, where a short stretch of nucleotides are exposed on
each strand of the double helix.
16. TRANSCRIPTION TERMINATION
• Once a gene is transcribed, the prokaryotic polymerase needs to
be instructed to dissociate from the DNA template and liberate
the newly made mRNA.
• Depending on the gene being transcribed, there are two kinds of
termination signals. One is protein-based and the other is RNA-
based.
• There are specific signals for the termination of transcription
(stop). Terminators are:
1. Rho-independent terminator (type 1 terminator)/
Intrinsic termination
2. Rho-dependent terminator (type 2 terminator)
17. • Rho-dependent terminators were first discovered
in bacteriophage genomes.
• Rho's key function is its helicase activity, for which energy is provided
by an RNA-dependent ATP hydrolysis.
• The initial binding site for Rho is an extended (~70 nucleotides,
sometimes 80–100 nucleotides) single-stranded region, rich
in cytosine and poor in guanine, called the rho utilisation site (rut).
• Rho binds to RNA and then uses its ATPase activity to provide the
energy to translocate along the RNA until it reaches the RNA–DNA
helical region, where it unwinds the hybrid duplex structure. small
mutations in the sequence disrupts its function.
• In short, Rho factor acts as an ATP-dependent unwinding enzyme,
moving along the newly forming RNA molecule towards its 3′
end and unwinding it from the DNA template as it proceeds.
18. Rho-dependent termination
• Rho-dependent termination is controlled by the rho protein, which tracks
along behind the polymerase on the growing mRNA chain.
• Near the end of the gene, the polymerase encounters a run of G
nucleotides on the DNA template and it stalls.
• As a result, the rho protein collides with the polymerase. The interaction
with rho releases the mRNA from the transcription bubble.
19.
20. Rho-independent terminator (type 1 terminator)/
Intrinsic termination
• Intrinsic, or rho-independent termination, is a process
in prokaryotes to signal the end of transcription and release the newly
constructed RNA molecule.
• intrinsic termination does not require a special protein to signal for
termination and is controlled by the specific sequences of RNA.
• As the polymerase nears the end of the gene being transcribed, it
encounters a region rich in C–G nucleotides.
• The mRNA folds back on itself, and the complementary C–G
nucleotides bind together. The result in secondary structure a
stable hairpin also known as a Stem-loop.
21. • This RNA hairpin is followed by multiple uracil nucleotides.
• The bonds between uracil and adenine are very weak.
• A protein bound to RNA polymerase (nusA) binds to the stem-loop
structure tightly enough to cause the polymerase to temporarily stall.
This pausing of the polymerase coincides with transcription of the
poly-uracil sequence.
• The weak adenine-uracil bonds lower the energy of destabilization for
the RNA-DNA duplex, allowing it to unwind and dissociate from the
RNA polymerase and liberate the new mRNA transcript.
22. • The purpose function of intrinsic termination is to signal for the
dissociation of the ternary elongation complex (TEC), signaling the
end of a transcript in prokaryotes.