BRIGHT FIELD MICROSCOPY by SIVASANGARI SHANMUGAM
bRIGHT FIELD MICROSCOPY is also called a compound microscope. The name bright - field is derived from the fact that the specimen is dark and contrasted by the surrounding bright viewing field.
LIGHT MICROSCOPY by SIVASANGARI SHANMUGAM
The optical microscope, The functions of a light microscope is based on its ability to focus a beam of light through, which is very small and transparent, to produce an image.
BRIGHT FIELD MICROSCOPY by SIVASANGARI SHANMUGAM
bRIGHT FIELD MICROSCOPY is also called a compound microscope. The name bright - field is derived from the fact that the specimen is dark and contrasted by the surrounding bright viewing field.
LIGHT MICROSCOPY by SIVASANGARI SHANMUGAM
The optical microscope, The functions of a light microscope is based on its ability to focus a beam of light through, which is very small and transparent, to produce an image.
This presentation include information about electron microscope & types of electron microscope i.e. SEM (Scanning electron microscope) & TEM (Transmission electron microscope).
An electron microscope is a microscope that uses a beam of scattered electrons as a source of illumination. It is used to get information about structure, topology, morphology & composition of materials. It has many advantages. Basically there are 4 types of electron microscope but here we will discuss only 2 types.
Transmission electron microscopy is a microscopy technique in which a beam of electrons is transmitted through an ultra-thin specimen, interacting with the specimen as it passes through it. Its resolution & magnification is about 10,000,000x. There are 5 types of transmission electron microscope i.e. BFTEM (Bright field transmision electron microscope), DFTEM (Dark field transmission electron microscope), HRTEM (High resolution transmission electron microscope), EFTEM (Energy filtered transmission electron microscope), ED (Electron diffraction). there are 4 techniques of TEM i.e. negative staining, shadow casting, Freeze fracture replication, freeze etching. It has many applications e.g, for the study of Cancer research, virology, chemical industry, electronic structure etc.
A scanning electron microscope is a type of electron microscope that produces images of a sample by scanning it with a focused beam of electrons. Types of signals produce by SEM include secondary electrons, back scattered electrons, X-rays, light rays. There are many advantages of SEM e.g, Btter resolution, fast imaging easy to operate, work with low voltage etc.
Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy, along with the emerging field of X-ray microscopy.
1. MICROSCOPY - introduction + principle (Basics)Nethravathi Siri
Basics only
Microscopy is the technical field that uses microscopes to observe samples which are
not in the resolution range of the normal-unaided eye.
Microscope is a scientific-instrument consisting of magnifying lens that enables an
observer to view the minute features distinctly.
In greek, micro = small
skopein = to view.
Bright field microscopy, Principle and applicationsKAUSHAL SAHU
Introduction
History
Basic Component of Microscope
Light Microscopy
Types of Light Microscopy
What Are Bright Microscopy
Principle of Bright Microscope
Advantage
Disadvantage
Application
Conclusion
Reference
Electron microscope, principle and applicationKAUSHAL SAHU
Introduction
History
Resolution &Magnification of
Electron microscope
Types of electron microscope
1) Transmission electron microscope (TEM)
- Structural parts of TEM
- Principle & Working of TEM
- Sample preparation for TEM
- Advantages & disadvantages of TEM
Scanning electron microscope (SEM)
- Structural parts of SEM
- Principle & Working of SEM
- Sample preparation for SEM
- Advantages & disadvantages of SEM
3) Scanning transmission electron microscope (STEM)
Applications of electron microscope
Conclusion
References
MICROMETRY is a technique used to measure the size of microscopic objects.It is based on principle of Calibration of the ocular micrometer using the stage micrometer. If you change microscope, the calibration process must be done again for each of the objective lenses that you are using because the magnification is different on different microscopes.
5. Microsocope ELECTRON MICROSCOPE (TEM & SEM ) - BasicsNethravathi Siri
Basics only
Electron beam is the source of illumination.
Image is produced by magnetic field.
Contrasting features between light microscope and electron microscope are
construction, working principle, specimen preparation, cost-expenses and designed
room (vacuum chamber).
Autoradiography is a bioanalytical technique that is used to visualize the radioactively labelled substances or molecules or or fragments of molecules by using X-ray films or photographic emulsions.
An isotope is one of two or more atoms having the same atomic number but different mass numbers.
Unstable isotopes are called Radioisotopes.
uses of radioisotopes are many which are discussed in this slide.
This presentation include information about electron microscope & types of electron microscope i.e. SEM (Scanning electron microscope) & TEM (Transmission electron microscope).
An electron microscope is a microscope that uses a beam of scattered electrons as a source of illumination. It is used to get information about structure, topology, morphology & composition of materials. It has many advantages. Basically there are 4 types of electron microscope but here we will discuss only 2 types.
Transmission electron microscopy is a microscopy technique in which a beam of electrons is transmitted through an ultra-thin specimen, interacting with the specimen as it passes through it. Its resolution & magnification is about 10,000,000x. There are 5 types of transmission electron microscope i.e. BFTEM (Bright field transmision electron microscope), DFTEM (Dark field transmission electron microscope), HRTEM (High resolution transmission electron microscope), EFTEM (Energy filtered transmission electron microscope), ED (Electron diffraction). there are 4 techniques of TEM i.e. negative staining, shadow casting, Freeze fracture replication, freeze etching. It has many applications e.g, for the study of Cancer research, virology, chemical industry, electronic structure etc.
A scanning electron microscope is a type of electron microscope that produces images of a sample by scanning it with a focused beam of electrons. Types of signals produce by SEM include secondary electrons, back scattered electrons, X-rays, light rays. There are many advantages of SEM e.g, Btter resolution, fast imaging easy to operate, work with low voltage etc.
Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy, along with the emerging field of X-ray microscopy.
1. MICROSCOPY - introduction + principle (Basics)Nethravathi Siri
Basics only
Microscopy is the technical field that uses microscopes to observe samples which are
not in the resolution range of the normal-unaided eye.
Microscope is a scientific-instrument consisting of magnifying lens that enables an
observer to view the minute features distinctly.
In greek, micro = small
skopein = to view.
Bright field microscopy, Principle and applicationsKAUSHAL SAHU
Introduction
History
Basic Component of Microscope
Light Microscopy
Types of Light Microscopy
What Are Bright Microscopy
Principle of Bright Microscope
Advantage
Disadvantage
Application
Conclusion
Reference
Electron microscope, principle and applicationKAUSHAL SAHU
Introduction
History
Resolution &Magnification of
Electron microscope
Types of electron microscope
1) Transmission electron microscope (TEM)
- Structural parts of TEM
- Principle & Working of TEM
- Sample preparation for TEM
- Advantages & disadvantages of TEM
Scanning electron microscope (SEM)
- Structural parts of SEM
- Principle & Working of SEM
- Sample preparation for SEM
- Advantages & disadvantages of SEM
3) Scanning transmission electron microscope (STEM)
Applications of electron microscope
Conclusion
References
MICROMETRY is a technique used to measure the size of microscopic objects.It is based on principle of Calibration of the ocular micrometer using the stage micrometer. If you change microscope, the calibration process must be done again for each of the objective lenses that you are using because the magnification is different on different microscopes.
5. Microsocope ELECTRON MICROSCOPE (TEM & SEM ) - BasicsNethravathi Siri
Basics only
Electron beam is the source of illumination.
Image is produced by magnetic field.
Contrasting features between light microscope and electron microscope are
construction, working principle, specimen preparation, cost-expenses and designed
room (vacuum chamber).
Autoradiography is a bioanalytical technique that is used to visualize the radioactively labelled substances or molecules or or fragments of molecules by using X-ray films or photographic emulsions.
An isotope is one of two or more atoms having the same atomic number but different mass numbers.
Unstable isotopes are called Radioisotopes.
uses of radioisotopes are many which are discussed in this slide.
Microscopy - Magnification, Resolving power, Principles, Types and ApplicationsNethravathi Siri
Magnification, Resolving power, Principles and Applications of Simple, Compound, Stereozoom, Phase contrast, Fluorescent and Electron microscopes (TEM & SEM).
Microscopy is the technical field that uses microscopes to observe samples which are not in the resolution range of the normal-unaided eye.
Types of Light Microscopes used in Histological Studies.pptxssuserab552f
Light microscopes relies on glass lenses and visible light to magnify tissue samples. It was
invented in XVII century, and has been improved over the years, resulting in the powerful
modern light microscopes. As individual cellular structures are too small to be seen by the
human eye, microscopy techniques have played a key role in the development of
histological techniques.
Eye microscopy and electron microscopy include differentiation and reflection. Retraction of magnetic fields/electron beams that interact with the image. As well as the scattering of scattered rays or other signals to create the image.
This procedure can be done by inserting a wide-field light sample or by scanning a fine beam over the sample. A microscopy scan probe involves. The interaction of the scanning probe with the surface of the object of interest.
Advances in microscopy transformed living things and exposed the field of histology. And so remain an important strategy for health and natural science.
X-ray microscopy is three-dimensional and unobtrusive. Allowing for repeated photographing of the same sample in situ or 4D subjects. And provides the ability to "see". The sample is readable before devoting it to advanced correction techniques.
The 3D X-ray microscope uses a computed tomography technique, rotating the sample. By 360 degrees and reconstructing images. CT is usually done with a flat panel display. The 3D X-ray microscope uses a series of objectives, e.g., from 4X to 40X, and can include a flat panel.
History of Microscopy
The field of the microscope dates back to at least the 17th century. Early mirrors, single-lens magnifying glasses with limited size. Back to the widespread use of eyeglasses in the 13th century. But the most advanced microscopes first appeared in Europe around 1620 Early.
Microscope doctors included Galileo Galilei, who was discovered in 1610. That he could turn off his telescope to see small objects nearby. And Cornelis Drebbel. Who may have invented the compact microscope in about 1620?
Antonie van Leeuwenhoek developed a simple magnifying microscope. In the 1670s and is often regarded as the first acclaimed microscopist and microbiologist.
Microscope Uses
to view bacteria, parasites, and a variety of human/animal cells
cellular process, cell division
DNA replication
tissue analysis
examining forensic evidence
studying the role of a protein within a cell
studying atomic structures
And in what way are bacteria able to infect human cells, then we use a microscope to study them all. Those studies are done at the micro-level.
We use a microscope to perform the kind of study that we cannot see with the naked eye.
Microscope component
Light
Lence
Optical/Light Microscopy
Bright Field Microscopy
Dark Field Microscopy
Confocal Microscopy
Phase Contrast Microscopy
Fluorescence Microscopy
Electron microscopy
Transmission Electron Microscopy
Scanning Electron Microscopy
Scanning Probe Microscopy
The resolving power of a microscope means
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
The Indian economy is classified into different sectors to simplify the analysis and understanding of economic activities. For Class 10, it's essential to grasp the sectors of the Indian economy, understand their characteristics, and recognize their importance. This guide will provide detailed notes on the Sectors of the Indian Economy Class 10, using specific long-tail keywords to enhance comprehension.
For more information, visit-www.vavaclasses.com
The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
In this webinar you will learn how your organization can access TechSoup's wide variety of product discount and donation programs. From hardware to software, we'll give you a tour of the tools available to help your nonprofit with productivity, collaboration, financial management, donor tracking, security, and more.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
Ethnobotany and Ethnopharmacology:
Ethnobotany in herbal drug evaluation,
Impact of Ethnobotany in traditional medicine,
New development in herbals,
Bio-prospecting tools for drug discovery,
Role of Ethnopharmacology in drug evaluation,
Reverse Pharmacology.
1. Principles and application
of light microscopy
• Presented by:-
• Gosai Rajeshwariben v.
• M.sc se m-2 (BOTANY)
• Paper –CBO 406
• Department of life sciences,
HNGU Patan.
3. Light passes unhindered and deviated through
specimens.
The light is projected by the objective across the
image plane.
Destructive and constructive interference results
in bright and dark areas.
4. > The greater number of higher diffracted order
admitted, the smaller the details that can be
resolved.
5. Light is produced from either an internal or
external light source and passes through the
iris diaphragm, a hole variable size which
controls the amount of light reaching the
specimen.
The light then passes through the condenser
which focuses the light onto the specimen.
6. >The slide is held on the stage at 90 degrees to
the path of light which next travels through
the specimen.
The objective lens magnifies the image of the
specimen before the light travels through
the barrel of the microscope.
The light finally passes through the eyepieces
lens and into the viewer’s eye which sends
impulses to the brain which in turn interprets
the image.
8. > Light microscopes play a large part in today’s
biology.
Biologists use microscopes to observe objects
and details at a cellular level to learn more
about the building blocks of all organisms.
Microscopes are also used to observe real time
movement in cells and organisms.
9. > Lastly , microscopes are used in biology to study
diseases like cancer and AIDS to help diagnose
the disease in patients and to help find a cure
for them.
Microscopes are used when studying light and
optics to learn how light refracts through
converging lenses and how a combination of
different lenses with varying focal lengths
affects the properties of the image.
10. > Microscopes are used in schools throughout
north America as part of the technology
section of the curriculum.
Microscopes provide students with an
understanding of real cells and their
supporting structures.
Also, microscopes provide students who are
inclined towards the medical field a more
intense look at the career choice and
develop basic skills.
11. Lastly, microscopes are used in forensics to
to help solve many crimes.
Often times, there will be human evidences
left on the crime scene.
This allows forensic scientists to examine
the evidence under a microscope and match
the results with a database to find the
culprit.
12. Mineralogists also use light microscopy,
typically with a special preparation of a
sample called thin section.
As the name implies, thin section are very
thin slices of a rock. The sample needs to
be thin enough for light to travel through
from the light source to the user’s eye.
The thin section will allow the shape of
different crystal grains to be seen.
13. Light microscopes are very much an ‘easy to
learn, hard to master’ type of equipment.
The microscope can be used with different
techniques, like epifluorescence and phase
contrast..