The document provides an overview of light microscopes, including their history and key components. It discusses how the first microscopes were developed in the 1600s using simple lenses. Modern light microscopes use lenses to magnify specimens up to 1000x their actual size and include features like brightfield, darkfield, phase contrast and fluorescence microscopy. Brightfield microscopes produce a dark image on a bright background while darkfield shows bright specimens on a dark background. Microscopy has many applications in diagnostic microbiology like rapid identification of pathogens and determination of clinical significance.
BRIGHT FIELD MICROSCOPY by SIVASANGARI SHANMUGAM
bRIGHT FIELD MICROSCOPY is also called a compound microscope. The name bright - field is derived from the fact that the specimen is dark and contrasted by the surrounding bright viewing field.
Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy, along with the emerging field of X-ray microscopy.
BRIGHT FIELD MICROSCOPY by SIVASANGARI SHANMUGAM
bRIGHT FIELD MICROSCOPY is also called a compound microscope. The name bright - field is derived from the fact that the specimen is dark and contrasted by the surrounding bright viewing field.
Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy, along with the emerging field of X-ray microscopy.
this presentation deals with the introduction of some of the commonly used optical microscopes in forensic labs; compound microscope, stereoscopic microscope, comparison microscope, fluorescence microscope and polarized microscope.
DARK FIELD MICROSCOPY by SIVASANGARI SHANMUGAM
Dark-field microscopy is ideally used to illuminate unstained samples causing them to appear brightly lit against a dark background.
This type of microscope contains a special condenser that scatters light and causes it to reflect off the specimen at an angle
Bright field microscopy, Principle and applicationsKAUSHAL SAHU
Introduction
History
Basic Component of Microscope
Light Microscopy
Types of Light Microscopy
What Are Bright Microscopy
Principle of Bright Microscope
Advantage
Disadvantage
Application
Conclusion
Reference
Electron microscope, principle and applicationKAUSHAL SAHU
Introduction
History
Resolution &Magnification of
Electron microscope
Types of electron microscope
1) Transmission electron microscope (TEM)
- Structural parts of TEM
- Principle & Working of TEM
- Sample preparation for TEM
- Advantages & disadvantages of TEM
Scanning electron microscope (SEM)
- Structural parts of SEM
- Principle & Working of SEM
- Sample preparation for SEM
- Advantages & disadvantages of SEM
3) Scanning transmission electron microscope (STEM)
Applications of electron microscope
Conclusion
References
3. Microscope simple, compound & stereo - BasicsNethravathi Siri
Basics only
A compound microscope is an optical instrument used to observe the magnified images of small objects on a glass slide. Compound microscopes are so called because they are designed with a compound lens system.
LIGHT MICROSCOPY by SIVASANGARI SHANMUGAM
The optical microscope, The functions of a light microscope is based on its ability to focus a beam of light through, which is very small and transparent, to produce an image.
this presentation deals with the introduction of some of the commonly used optical microscopes in forensic labs; compound microscope, stereoscopic microscope, comparison microscope, fluorescence microscope and polarized microscope.
DARK FIELD MICROSCOPY by SIVASANGARI SHANMUGAM
Dark-field microscopy is ideally used to illuminate unstained samples causing them to appear brightly lit against a dark background.
This type of microscope contains a special condenser that scatters light and causes it to reflect off the specimen at an angle
Bright field microscopy, Principle and applicationsKAUSHAL SAHU
Introduction
History
Basic Component of Microscope
Light Microscopy
Types of Light Microscopy
What Are Bright Microscopy
Principle of Bright Microscope
Advantage
Disadvantage
Application
Conclusion
Reference
Electron microscope, principle and applicationKAUSHAL SAHU
Introduction
History
Resolution &Magnification of
Electron microscope
Types of electron microscope
1) Transmission electron microscope (TEM)
- Structural parts of TEM
- Principle & Working of TEM
- Sample preparation for TEM
- Advantages & disadvantages of TEM
Scanning electron microscope (SEM)
- Structural parts of SEM
- Principle & Working of SEM
- Sample preparation for SEM
- Advantages & disadvantages of SEM
3) Scanning transmission electron microscope (STEM)
Applications of electron microscope
Conclusion
References
3. Microscope simple, compound & stereo - BasicsNethravathi Siri
Basics only
A compound microscope is an optical instrument used to observe the magnified images of small objects on a glass slide. Compound microscopes are so called because they are designed with a compound lens system.
LIGHT MICROSCOPY by SIVASANGARI SHANMUGAM
The optical microscope, The functions of a light microscope is based on its ability to focus a beam of light through, which is very small and transparent, to produce an image.
Research is "creative and systematic work undertaken to increase the stock of knowledge". It involves the collection, organization and analysis of evidence to increase understanding of a topic, characterized by a particular attentiveness to controlling sources of bias and error.
Types of Microscopes with their applications - Microbiologynote.com
https://microbiologynote.com/types-of-microscopes-with-their-applications/
Youtube Lecture Video:
https://www.youtube.com/watch?v=nuJZtXohFFQ&ab_channel=MicrobiologyNote
In the late 16th century several Dutch lens makers designed devices that magnified objects, but in 1609 Galileo Galilei perfected the first device known as a microscope. Dutch spectacle makers Zaccharias Janssen and Hans Lipperhey are noted as the first men to develop the concept of the compound microscope.
Malaria is a life-threatening disease caused by parasites that are transmitted to people through the bites of infected female Anopheles mosquitoes. It is one of the world’s leading causes of death, particularly among children in developing countries.
Introduction Malaria
Chronology in Malaria
Epidemology
Life cycle
Pathogenesis and clinical feature
Lab Test
Treatment of Malaria
Prevention of Malaria
We understand the unique challenges pickleball players face and are committed to helping you stay healthy and active. In this presentation, we’ll explore the three most common pickleball injuries and provide strategies for prevention and treatment.
Antibiotic Stewardship by Anushri Srivastava.pptxAnushriSrivastav
Stewardship is the act of taking good care of something.
Antimicrobial stewardship is a coordinated program that promotes the appropriate use of antimicrobials (including antibiotics), improves patient outcomes, reduces microbial resistance, and decreases the spread of infections caused by multidrug-resistant organisms.
WHO launched the Global Antimicrobial Resistance and Use Surveillance System (GLASS) in 2015 to fill knowledge gaps and inform strategies at all levels.
ACCORDING TO apic.org,
Antimicrobial stewardship is a coordinated program that promotes the appropriate use of antimicrobials (including antibiotics), improves patient outcomes, reduces microbial resistance, and decreases the spread of infections caused by multidrug-resistant organisms.
ACCORDING TO pewtrusts.org,
Antibiotic stewardship refers to efforts in doctors’ offices, hospitals, long term care facilities, and other health care settings to ensure that antibiotics are used only when necessary and appropriate
According to WHO,
Antimicrobial stewardship is a systematic approach to educate and support health care professionals to follow evidence-based guidelines for prescribing and administering antimicrobials
In 1996, John McGowan and Dale Gerding first applied the term antimicrobial stewardship, where they suggested a causal association between antimicrobial agent use and resistance. They also focused on the urgency of large-scale controlled trials of antimicrobial-use regulation employing sophisticated epidemiologic methods, molecular typing, and precise resistance mechanism analysis.
Antimicrobial Stewardship(AMS) refers to the optimal selection, dosing, and duration of antimicrobial treatment resulting in the best clinical outcome with minimal side effects to the patients and minimal impact on subsequent resistance.
According to the 2019 report, in the US, more than 2.8 million antibiotic-resistant infections occur each year, and more than 35000 people die. In addition to this, it also mentioned that 223,900 cases of Clostridoides difficile occurred in 2017, of which 12800 people died. The report did not include viruses or parasites
VISION
Being proactive
Supporting optimal animal and human health
Exploring ways to reduce overall use of antimicrobials
Using the drugs that prevent and treat disease by killing microscopic organisms in a responsible way
GOAL
to prevent the generation and spread of antimicrobial resistance (AMR). Doing so will preserve the effectiveness of these drugs in animals and humans for years to come.
being to preserve human and animal health and the effectiveness of antimicrobial medications.
to implement a multidisciplinary approach in assembling a stewardship team to include an infectious disease physician, a clinical pharmacist with infectious diseases training, infection preventionist, and a close collaboration with the staff in the clinical microbiology laboratory
to prevent antimicrobial overuse, misuse and abuse.
to minimize the developme
CHAPTER 1 SEMESTER V PREVENTIVE-PEDIATRICS.pdfSachin Sharma
This content provides an overview of preventive pediatrics. It defines preventive pediatrics as preventing disease and promoting children's physical, mental, and social well-being to achieve positive health. It discusses antenatal, postnatal, and social preventive pediatrics. It also covers various child health programs like immunization, breastfeeding, ICDS, and the roles of organizations like WHO, UNICEF, and nurses in preventive pediatrics.
Navigating the Health Insurance Market_ Understanding Trends and Options.pdfEnterprise Wired
From navigating policy options to staying informed about industry trends, this comprehensive guide explores everything you need to know about the health insurance market.
India Clinical Trials Market: Industry Size and Growth Trends [2030] Analyzed...Kumar Satyam
According to TechSci Research report, "India Clinical Trials Market- By Region, Competition, Forecast & Opportunities, 2030F," the India Clinical Trials Market was valued at USD 2.05 billion in 2024 and is projected to grow at a compound annual growth rate (CAGR) of 8.64% through 2030. The market is driven by a variety of factors, making India an attractive destination for pharmaceutical companies and researchers. India's vast and diverse patient population, cost-effective operational environment, and a large pool of skilled medical professionals contribute significantly to the market's growth. Additionally, increasing government support in streamlining regulations and the growing prevalence of lifestyle diseases further propel the clinical trials market.
Growing Prevalence of Lifestyle Diseases
The rising incidence of lifestyle diseases such as diabetes, cardiovascular diseases, and cancer is a major trend driving the clinical trials market in India. These conditions necessitate the development and testing of new treatment methods, creating a robust demand for clinical trials. The increasing burden of these diseases highlights the need for innovative therapies and underscores the importance of India as a key player in global clinical research.
The dimensions of healthcare quality refer to various attributes or aspects that define the standard of healthcare services. These dimensions are used to evaluate, measure, and improve the quality of care provided to patients. A comprehensive understanding of these dimensions ensures that healthcare systems can address various aspects of patient care effectively and holistically. Dimensions of Healthcare Quality and Performance of care include the following; Appropriateness, Availability, Competence, Continuity, Effectiveness, Efficiency, Efficacy, Prevention, Respect and Care, Safety as well as Timeliness.
2. • The evolution of the Microbiology field put to
perspective the need to identify, view, observe
and understand microorganisms, including
their structural morphologies and
mechanisms.
3. History
• The first simple microscope was discovered
by two Dutch scientists, Zaccharias Janssen
and his father, Hans who made spectacles,
were the first to experiment with their lenses
by combining lenses in a tube and observed
that the objects that were nearby, appeared
closer and larger.
4. History
• Antony Van Lewnehoueek an amateur
Microbiologist made the first simple
microscope, that enabled him to observe the
presence of tiny living organisms in pond
water that appeared like dots. His simple
microscope was made up of a double convex
glass lens that was held between two silver
plates.
6. What is a light microscope?
• Instrument or tool, that uses visible light to
detect and magnify very small objects and
enlarge them.
• Use lenses to focus light on the specimen,
magnifying it thus producing an image
7. Principles of Light Microscopy
• For light microscopy, visible light is passed
through the specimen and then through a
series of lenses that bend the light in a
manner that results in magnification of the
organisms present in the specimen . The total
magnification achieved is the product of the
lenses used.
8.
9. Key component to light microscopy
Magnification
• Objective lens magnifies objects 100× (times)
• the ocular lens magnifies 10×.
• Using these two lenses in combination,
organisms in the specimen are magnified
1000× their actual size when viewed through
the ocular lens.
10. Contrast
• Needed to make objects stand out from the
background
• Contrast is most commonly achieved by
staining techniques that highlight organisms
and allow them to be differentiated from one
another and from background material and
debris.
11. Resolution
• To optimize visualization, other factors besides
magnification must be considered.
• Resolution, defined as the extent to which
detail in the magnified object is maintained, is
also essential.
12. Types of microscopes
Depending on the number of lenses, there are
two types of microscopes
• Simple light microscope (it has low
magnification because it uses a single lens)
• Compound light microscope (it has a higher
magnification compared to the simple
microscope because it uses at least two sets of
lenses, an objective lens, and an eyepiece).
13. Light microscope
The modern types of Light Microscopes include:
• Bright field light microscope
• Dark field light microscope
• Phase contrast light microscope
• Fluorescence light microscope
14. Bright field microscope
• most basic optical Microscope
• produces a dark image against a bright
background
• provide a high-resolution image
16. • Objective lens: magnifies the image by 10x, 20x,
40x and 100x
• Ocular lens: magnifies the image by 10x
• Condenser: focuses a beam of light onto the
specimen
• Diaphragm: controls the diameter of the beam of
light that passes through the condenser
• Two focusing knobs: focus on the image and
sharpen the image.
17.
18. Applications of Microscopy in
Diagnostic Microbiology
• Rapid preliminary organism identification by direct visualization in patient
specimens
• Rapid final identification of certain organisms by direct visualization in patient
specimens
• Detection of different organisms present in the same specimen
• Detection of organisms not easily cultivated in the laboratory
• Evaluation of patient specimens for the presence of cells indicative of
inflammation (i.e., phagocytes) or contamination (i.e., squamous epithelial cells)
• Determination of an organism’s clinical significance; bacterial contaminants usually
are not present in patient specimens at sufficiently high numbers (×105 cells/mL)
to be seen by light microscopy
• Provide preculture information about which organisms might be expected to grow
so that appropriate cultivation techniques are used
• Determine which tests and methods should be used for identification and
characterization of cultivated organisms
• Provide a method for investigating unusual or unexpected laboratory test results
20. PRINCIPLE OF DARK GROUND
MICROSCOPE
• To view a specimen in dark field, an opaque
disc is placed underneath the condenser lens,
so that only light that is scattered by objects
on the slide can reach the eye
• Instead of coming up through the specimen,
the light is reflected by particles on the slide.
21.
22.
23. Application of Dark ground microscopy
• demonstration of very thin bacteria not visible under
ordinary illumination
• method for rapid demonstration of Treponema pallidum
• demonstration of the motility of flagellated bacteria and
protozoa.
• Darkfield is used to study marine organisms such as algae,
plankton, diatoms, insects, yeast and protozoa
• Darkfield is used to study mounted cells and tissues.
• It is more useful in examining external details, such as
outlines, edges, grain boundaries and surface defects than
internal structure.
•
24. Bright field vs Dark field Microscope
Bright field microscope Darkfield microscope
Bright field microscope formed a dark
image against bright field.
Darkfield Microscope formed a bright
image against dark background
Stained, fixed, and live specimens are
observed.
We can see the living and unstained cells.
In a bright-field microscope, only the
scattered lights are able to enter the
objective lens and transmitted lights or
unscattered light rays are omitted, that’s
why the viewer sees a dark image against
the brightfield.
The only light that has been reflected or
reflected by the specimen forms an
image.
Organism appear as dark Organism appear as bright
Background appear as bright Background appear as dark.
25. Bright field vs Dark field Microscope
Bright field Microscope Dark field Microscope
It shows us the morphological as well
as the internal structure of the
specimen.
It show us External structure of
specimen in great detail.
Bright fields are inexpensive Dark fields are expensive.
Specimen preparation or staining is a
complex and lengthy process. Takes
too much time.
No need stain.
Opaque disc is Absent Opaque disc is Present
It is easy to use.
operating procedure is not easy as
compared to bright field microscope.
36. Advantages
• helps in the study of cell behaviour.
• highlights the biomolecule of interest.
• highlight the image of particular structural
components within the microscopic organisms.
• highly sensitive technique that can detect around
50 molecules/µm3.
• analyze or track the physiochemical properties of
multiple biomolecules simultaneously.
37. • Disadvantages
• It only allows the observation of specific structures
inside a cell tagged with the fluorescent dye.
• The photobleaching due to the electron excitation
during the process of fluorescence may affect reactive
molecules of the fluorescent dyes. As a result, the
reactive dyes might lose their chemical property of
fluorescence emission intensity.
• The cells are susceptible to the phototoxic effect after
staining with fluorescent dyes, as the fluorophore
molecules absorb the high energy photons from the
short-wavelength light.
Microbiology’s scope is to study organisms and minute agents that can only be examined and observed with a microscope.
Objective lenses of lower magnification are available so that those of 10×, 20×, and 40× magnification power can provide total magnifications of 100×, 200×, and 400×, respectively. Magnification of 1000× allows for the visualization of fungi, most parasites, and most bacteria, but it is not sufficient for observing viruses, which require magnification of 100,000× or more (see Electron Microscopy in this chapter).
. Because microorganisms are essentially transparent, owing to their microscopic dimensions and high water content, they cannot be easily detected among the background materials and debris in patient specimens.
In the absence of staining, the simplest way to improve contrast is to reduce the diameter of the microscope aperture diaphragm increasing contrast at the expense of the resolution