MICROMETRY is a technique used to measure the size of microscopic objects.It is based on principle of Calibration of the ocular micrometer using the stage micrometer. If you change microscope, the calibration process must be done again for each of the objective lenses that you are using because the magnification is different on different microscopes.
Microscopy - Magnification, Resolving power, Principles, Types and ApplicationsNethravathi Siri
Magnification, Resolving power, Principles and Applications of Simple, Compound, Stereozoom, Phase contrast, Fluorescent and Electron microscopes (TEM & SEM).
Microscopy is the technical field that uses microscopes to observe samples which are not in the resolution range of the normal-unaided eye.
microorganisms are microscopic objects that are visible only with the help of a microscope. micrometry refers to the measurement of dimensions of the desired microorganisms. ocular micrometer and stage micrometer.
This presentation include information about electron microscope & types of electron microscope i.e. SEM (Scanning electron microscope) & TEM (Transmission electron microscope).
An electron microscope is a microscope that uses a beam of scattered electrons as a source of illumination. It is used to get information about structure, topology, morphology & composition of materials. It has many advantages. Basically there are 4 types of electron microscope but here we will discuss only 2 types.
Transmission electron microscopy is a microscopy technique in which a beam of electrons is transmitted through an ultra-thin specimen, interacting with the specimen as it passes through it. Its resolution & magnification is about 10,000,000x. There are 5 types of transmission electron microscope i.e. BFTEM (Bright field transmision electron microscope), DFTEM (Dark field transmission electron microscope), HRTEM (High resolution transmission electron microscope), EFTEM (Energy filtered transmission electron microscope), ED (Electron diffraction). there are 4 techniques of TEM i.e. negative staining, shadow casting, Freeze fracture replication, freeze etching. It has many applications e.g, for the study of Cancer research, virology, chemical industry, electronic structure etc.
A scanning electron microscope is a type of electron microscope that produces images of a sample by scanning it with a focused beam of electrons. Types of signals produce by SEM include secondary electrons, back scattered electrons, X-rays, light rays. There are many advantages of SEM e.g, Btter resolution, fast imaging easy to operate, work with low voltage etc.
MICROMETRY is a technique used to measure the size of microscopic objects.It is based on principle of Calibration of the ocular micrometer using the stage micrometer. If you change microscope, the calibration process must be done again for each of the objective lenses that you are using because the magnification is different on different microscopes.
Microscopy - Magnification, Resolving power, Principles, Types and ApplicationsNethravathi Siri
Magnification, Resolving power, Principles and Applications of Simple, Compound, Stereozoom, Phase contrast, Fluorescent and Electron microscopes (TEM & SEM).
Microscopy is the technical field that uses microscopes to observe samples which are not in the resolution range of the normal-unaided eye.
microorganisms are microscopic objects that are visible only with the help of a microscope. micrometry refers to the measurement of dimensions of the desired microorganisms. ocular micrometer and stage micrometer.
This presentation include information about electron microscope & types of electron microscope i.e. SEM (Scanning electron microscope) & TEM (Transmission electron microscope).
An electron microscope is a microscope that uses a beam of scattered electrons as a source of illumination. It is used to get information about structure, topology, morphology & composition of materials. It has many advantages. Basically there are 4 types of electron microscope but here we will discuss only 2 types.
Transmission electron microscopy is a microscopy technique in which a beam of electrons is transmitted through an ultra-thin specimen, interacting with the specimen as it passes through it. Its resolution & magnification is about 10,000,000x. There are 5 types of transmission electron microscope i.e. BFTEM (Bright field transmision electron microscope), DFTEM (Dark field transmission electron microscope), HRTEM (High resolution transmission electron microscope), EFTEM (Energy filtered transmission electron microscope), ED (Electron diffraction). there are 4 techniques of TEM i.e. negative staining, shadow casting, Freeze fracture replication, freeze etching. It has many applications e.g, for the study of Cancer research, virology, chemical industry, electronic structure etc.
A scanning electron microscope is a type of electron microscope that produces images of a sample by scanning it with a focused beam of electrons. Types of signals produce by SEM include secondary electrons, back scattered electrons, X-rays, light rays. There are many advantages of SEM e.g, Btter resolution, fast imaging easy to operate, work with low voltage etc.
1. MICROSCOPY - introduction + principle (Basics)Nethravathi Siri
Basics only
Microscopy is the technical field that uses microscopes to observe samples which are
not in the resolution range of the normal-unaided eye.
Microscope is a scientific-instrument consisting of magnifying lens that enables an
observer to view the minute features distinctly.
In greek, micro = small
skopein = to view.
LIGHT MICROSCOPY by SIVASANGARI SHANMUGAM
The optical microscope, The functions of a light microscope is based on its ability to focus a beam of light through, which is very small and transparent, to produce an image.
BRIGHT FIELD MICROSCOPY by SIVASANGARI SHANMUGAM
bRIGHT FIELD MICROSCOPY is also called a compound microscope. The name bright - field is derived from the fact that the specimen is dark and contrasted by the surrounding bright viewing field.
An isotope is one of two or more atoms having the same atomic number but different mass numbers.
Unstable isotopes are called Radioisotopes.
uses of radioisotopes are many which are discussed in this slide.
Cyanobacteria are important in the nitrogen cycle.
Cyanobacteria are very important organisms for the health and growth of many plants. They are one of very few groups of organisms that can convert inert atmospheric nitrogen into an organic form, such as nitrate or ammonia.
Centrifugation principle and types by Dr. Anurag YadavDr Anurag Yadav
concept of cnetrifugation,
basic Principle
centrifugal force
types of centrifugation based on use and rotor type
application of the each type of centrifuge
Ultracentrifuge in detail
application in general
1. MICROSCOPY - introduction + principle (Basics)Nethravathi Siri
Basics only
Microscopy is the technical field that uses microscopes to observe samples which are
not in the resolution range of the normal-unaided eye.
Microscope is a scientific-instrument consisting of magnifying lens that enables an
observer to view the minute features distinctly.
In greek, micro = small
skopein = to view.
LIGHT MICROSCOPY by SIVASANGARI SHANMUGAM
The optical microscope, The functions of a light microscope is based on its ability to focus a beam of light through, which is very small and transparent, to produce an image.
BRIGHT FIELD MICROSCOPY by SIVASANGARI SHANMUGAM
bRIGHT FIELD MICROSCOPY is also called a compound microscope. The name bright - field is derived from the fact that the specimen is dark and contrasted by the surrounding bright viewing field.
An isotope is one of two or more atoms having the same atomic number but different mass numbers.
Unstable isotopes are called Radioisotopes.
uses of radioisotopes are many which are discussed in this slide.
Cyanobacteria are important in the nitrogen cycle.
Cyanobacteria are very important organisms for the health and growth of many plants. They are one of very few groups of organisms that can convert inert atmospheric nitrogen into an organic form, such as nitrate or ammonia.
Centrifugation principle and types by Dr. Anurag YadavDr Anurag Yadav
concept of cnetrifugation,
basic Principle
centrifugal force
types of centrifugation based on use and rotor type
application of the each type of centrifuge
Ultracentrifuge in detail
application in general
i am HAFIZ M WASEEM from mailsi vehari
BSc in science college Multan Pakistan
MSC university of education Lahore Pakistan
I love Pakistan and my teachers
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Nucleophilic Addition of carbonyl compounds.pptxSSR02
Nucleophilic addition is the most important reaction of carbonyls. Not just aldehydes and ketones, but also carboxylic acid derivatives in general.
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2. Introduction:
Microorganisms are microscopic
objects that are visible only with the help of
a microscope. Sometimes it is necessary to
measure its dimensions for its identification
process. But determination of the size of a
microorganism is not an easy process.
3. Micrometry:
Micrometry refers to the measurement of
dimensions of the desired microorganisms
under a microscope which uses two micro-
scale known as micrometers. These are two
types
4. Types of Micrometry:
There are usually two types of
micrometers
Stage micrometer.
Ocular meter or ocular micrometer.
5. Stage micrometer:
The measurement on the stage of the
microscope where an object is to be kept. This
micrometer is of a slide’s shape and size and has a
mount of very finely graduated scale. In the centre of
the stage micrometer a known 1mm distance is
etched into 100 equally spaced divisions making
each divisions equals 0.01mm or 10micrometer.
1mm=100 division
1mm=1000mu
6. Ocular micrometer:
ocular micrometer with microscopic
graduations etched on their surface is a circular
glass disc that fits into the circular shelf inside
the eyepiece of the microscope.
It has 100 equally spaced divisions marked 0-
10. depending on the objective being used the
distance between these graduations will very
that determines the size of the field.
7. STAGE MICROMETER WORK FLOW:
The stage micrometer kept under the low
power microscope and is observed through the eye
piece having eyepiece reticle.
at this magnification the number of eyepiece
reticule coinciding the stage micrometer are
observed and thence calculated for microns per
ocular divisions.
8. The correct way to calibrate a
reticle with a stage micrometer is to
line up the left edges of each.
For ex;6 ocular division is
coincide with 8 division of stage
micrometer.
9. One ocular division = number of stage meter division
number of ocular meter division
=0.08/6
=0.08*1000/6
=13.3mu
10.
11. Ocular micrometer works flow:
Insert the ocular meter in the eye piece of
your calibrated microscope by unscrewing its
upper eye lens.
Keep the spore or bacterial slide to be
measured on the stage of the microscope and
observe through the eyepiece containing ocular
meter.
The number of ocular divisions covered by
the microbe is counted by viewing through he
eye piece.
12. The size of the microorganism is
determined by multiplying the
number of ocular divisions covered
by the microbe with the calibration
factor.
15. Application:
This method is more important in
microbiological laboratory.
The main purpose of this method to
identified the length if the organism
such as bacteria, virus, human RBC
cells, yeast cell etc..