It is defined simply as a technique to efficiently and stably introduce foreign genes into the genome of target cells.
The insertion of unrelated, therapeutic genetic information in the form of DNA into target cells
It is defined simply as a technique to efficiently and stably introduce foreign genes into the genome of target cells.
The insertion of unrelated, therapeutic genetic information in the form of DNA into target cells
There is the fifth video by Miss Aymen Arif Sindh Biotechnologist Association has taken initiative for all young scientists, researchers, and students to have the platform to show their talent and interest in different activities.
Topic: Plasmids and its types
Presentation by: Aymen Arif
Research Officer at Halal Food and testing Laboratory,
Industrial Analytical Center, H.E.J (ICCBS).
Youtube: https://www.youtube.com/watch?v=-spdnc-2z6Q
Autonomously replicating circular fragment present in DNA is called plasmids.
The term plasmid was first introduced by American molecular biologist Joshua Lederberg in1952.
An episome is a plasmid capable of inserting DNA into the host chromosome.
Because of their ability to transfer DNA from one bacterium to another, plasmids are extensively used in recombinant DNA technology or genetic engineering.
BAC & YAC are artificially prepared chromosomes to clone DNA sequences.yeast artificial chromosome is capable of carrying upto 1000 kbp of inserted DNA sequence
MBB 501 PLANT BIOTECHNOLOGY
INFORMATION ABOUT DIFFERENT DNA MODIFYING ENZYMES
WHAT IS AN ENZYME?
Alkaline Phosphatase
Polynucleotide kinase
Terminal deoxyneucleotidyl transferase
Nucleases
Exonuclease
Bal31 Exonuclease III
Endonuclease
S1 endonulease
Deoxyribonuclease 1 (Dnase 1)
RNase A
RNase H
Restriction Endonuclease
PvuI
PvuII
Different types of endonuclease enzymes
The recognition sequences for some of the most frequently used restriction endonucleases.
Categorization of enzymes
Isoschizomers
Neoschizomers
Isocaudomers
description of plasmids and types and importance of plasmids and artificial plasmids(PBR322,cosmids,phagemids) and selection of the recombinants and uses and advantages and disadvantages of the plasmids
This lecture is intended as an introduction to the fundamental concepts associated with plasmid DNA. Plasmids can be applied as vectors in Genetic Engineering for the production of recombinant proteins as well as the construction of genomic libraries for DNA sequencing projects.
There is the fifth video by Miss Aymen Arif Sindh Biotechnologist Association has taken initiative for all young scientists, researchers, and students to have the platform to show their talent and interest in different activities.
Topic: Plasmids and its types
Presentation by: Aymen Arif
Research Officer at Halal Food and testing Laboratory,
Industrial Analytical Center, H.E.J (ICCBS).
Youtube: https://www.youtube.com/watch?v=-spdnc-2z6Q
Autonomously replicating circular fragment present in DNA is called plasmids.
The term plasmid was first introduced by American molecular biologist Joshua Lederberg in1952.
An episome is a plasmid capable of inserting DNA into the host chromosome.
Because of their ability to transfer DNA from one bacterium to another, plasmids are extensively used in recombinant DNA technology or genetic engineering.
BAC & YAC are artificially prepared chromosomes to clone DNA sequences.yeast artificial chromosome is capable of carrying upto 1000 kbp of inserted DNA sequence
MBB 501 PLANT BIOTECHNOLOGY
INFORMATION ABOUT DIFFERENT DNA MODIFYING ENZYMES
WHAT IS AN ENZYME?
Alkaline Phosphatase
Polynucleotide kinase
Terminal deoxyneucleotidyl transferase
Nucleases
Exonuclease
Bal31 Exonuclease III
Endonuclease
S1 endonulease
Deoxyribonuclease 1 (Dnase 1)
RNase A
RNase H
Restriction Endonuclease
PvuI
PvuII
Different types of endonuclease enzymes
The recognition sequences for some of the most frequently used restriction endonucleases.
Categorization of enzymes
Isoschizomers
Neoschizomers
Isocaudomers
description of plasmids and types and importance of plasmids and artificial plasmids(PBR322,cosmids,phagemids) and selection of the recombinants and uses and advantages and disadvantages of the plasmids
This lecture is intended as an introduction to the fundamental concepts associated with plasmid DNA. Plasmids can be applied as vectors in Genetic Engineering for the production of recombinant proteins as well as the construction of genomic libraries for DNA sequencing projects.
A bacterial plasmid is a short, usually circular, and double-stranded segment of DNA that is found in the cytoplasm separate from the main bacterial chromosome. This presentation contains plasmid features, replication, classification and its uses.
On the basis of need of specific content of any topic, i prepared a slides of plasmid for needy students. I'm also a student that's why i know how useful a proper presentation for us.
In this presentation, i try to cover some basic knowledge regarding to plasmid. If you like this ppt than please let me know, it gives me a motivation. If you need other topics ppt then write a topic name on comment section. THANK YOU
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
2. What is plasmid
• Plasmid is an extra chromosomal DNA molecule which
is capable of replicating independently from the
chromosomal DNA.
3. • They are found in numbers ranging from one per cell to
hundreds per cell (this is called "copy number").
4. • plasmids are not essential for the survival of
bacteria.
• but they may encode a wide variety of genetic
determinants, which permit their bacterial hosts
to survive better in an adverse environment or to
compete better with other microorganisms
occupying the same ecological environment.
5. • Plasmids vary in size, but most are between
1,000 to 25,000 base pairs.
• can be found in Bacteria and Eukarya.
6. Types of plasmids
There are five main classes :
1- Fertility- F-plasmids
They are capable of conjugation (transfer of genetic
material between bacteria ).
2- Resistance- R-plasmids
which contain genes that can build a resistance against
antibiotics or heavy–metal.
7. 3- Col-plasmids
which contain genes that code for bacteriocins, that can
kill other bacteria.
4- Degradative plasmids
which enable the digestion of unusual substances.
5- Virulence plasmids
which turn the bacterium into a pathogen.
8. Plasmid Exchange
• Bacteria can exchange or transfer plasmid between
other bacteria in three different ways.
• In every case the source cells of the plasmid are called
the
–DONORS-
and the cells that receive the plasmid are called the
–-RECIPIENTS-
9. The three forms of bacterial DNA exchange
are :
1) CONJUGATION
This process involves one bacterium making a copy of a
plasmid, and transferring that copy to another bacterium.
10. 2) TRANSFORMATION
This is the process in which a recipient cell takes up plasmid
DNA from the environment (such as DNA released from a dead
organism.
12. Conformations of plasmids
Plasmid DNA may appear
in different conformations,
like, coiled, Supercoiled,
circular and linear, which
(for a given size) run at
different speeds in a gel
during electrophoresis:
Supercoiled
13. Plasmid DNA extraction
Plasmid DNA can easily be purified away from
the rest of the genome. For their use as vectors
and for molecular cloning .
In recent times many commercial kits have been
created to perform plasmid extraction at various
scales . which are:
• minipreps
• medipreps
• maxipreps
14. Visualization of plasmid
• plasmid can be visualized on a gel after staining
with:
Ethidium bromide
and viewing the fluorescence pattern under an
ultraviolet light.
16. • Plasmids are extremely valuable tools in the fields of
molecular biology and genetics, specifically in the area of
genetic engineering where they are commonly used to
multiply (make many copies of ) particular genes .
• Plasmids in this conditions are called vectors .
Applications of plasmids
17. They play a critical role in :
gene cloning, recombinant protein production (e.g., of
human insulin).