Histone H2B is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone H2B is a structural protein that helps organise eukaryotic DNA. It plays an important role in the biology of the nucleus where it is involved in the packaging and maintaining of chromosomes, regulation of transcription, and replication and repair of DNA. Histone H2B helps regulate chromatin structure and function through post-translational modifications and specialised histone variants. The antibody detects endogenous Histone H2B (Tri Methyl Lys43) protein. Anti-Histone H2B (Tri Methyl Lys43) antibody - http://www.stjohnslabs.com/histone-h2b-tri-methyl-lys43-antibody?filter_name=STJ97165 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

1. Result
“Some Specificity detected. However due to low antibody concentration result was inconclusive. Peptide test on the
antibody is only showing weak signal.“ Q . Fang, Western University
Lane No. Antigen Loading amount Primary antibody
Primary antibody
dilution ratio
Secondary
antibody dilution
ratio
Visualiza-
tion time
1
H2BK43me3/2/1
/0 peptide
100 nM
STJ97165
H2BK43me3
antibody
1:1000 1:5000 120S
Materials for Validation: Short histone peptide
Method of validation: Peptide dot blotting
Primary Antibody:
STJ97165 H2BK43me3 anti-
body
Secondary Antibody HRP goat anti-rabbit
Reference antibody: Yenzym antibody
Dilution ratio: 1:1000
Protocol
Treatment of materials: 2ul of 100nM peptide was put onto nitrocellulose membrane
Air Dry: 15 mins.
Blocking: 1X TBST with 5% skimmed milk powder for 1 hr at room temperature.
Membrane wash: 10 minutes with TBS-T
Primary antibody incubation: Primary antibody incubated for 2 hours at room temperature.
Membrane wash: 3x 10 minutes with TBS-T.
Secondary antibody incubation: secondary antibody incubated for 1 hour at room temperature.
Membrane wash: 3x 10 minutes with TBS-T
Membrane incubation: Incubate the membrane with ELC reagent for 2 minutes at room temperature.
Antibody Customer Review:
STJ97165 Anti-H2BK43me3 antibody
Antibody Specificity:
Antibody Rating: