Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and maintenance. To purchase this antibody use the following link: http://www.stjohnslabs.com/tubulin-beta-antibody?filter_name=STJ31562

1. HOW DID OUR
ANTIBODY PERFORM?
CUSTOMER REVIEW: Tubulin-beta Polyclonal Antibody (STJ31562)

2. WHAT WAS USED?
Primary antibody: Tubulin-beta Polyclonal
Antibody (STJ31562)
Provider: St John's Laboratory
Dilution ratio: 1:100
Application: Immunofluorescence
Materials for validation: Rat primary hippocampal
neurons

3. WHAT WAS THE PROTOCOL?
Fixation:
1. Wash in PBS pH 7.4
2. Fixation in 4 % paraformaldehyde in 20 min, RT
3. Wash in PBS pH 7.4
4. Permabilization - PBS pH 7.4, 0.5 % triton X-100, 10 min
Labelling:
1. Anti tubulin-beta (1:100) in pH 7.4, 0.5 % triton X-100 1 hour, RT
2. 4 x 5 min wash in PBS pH 7.4, 0.5 % triton X-100
3. Alexa 488 goat anti-rabbit (1:500)
4. 4 x 5 min wash in PBS pH 7.4, 0.5 % triton X-100
5. 0-15 min with Hoechst
6. 2 x 2-5 min wash in water, dry sample and mount

4. WHAT DID THE CUSTOMER THINK?
Testing results were provided by Morten Nielsen from the
University of Aarhus. They stated that “I tested the
Tubulin-beta antibody in rat primary hippocampal
neurons, and it worked nicely for immunofluorescence.”

5. “WE INVITE YOU TO TEST OUR PRODUCTS”
Our aim is to not only connect our customers
with the best product, but to also give them
the opportunity to test the product’s
performance risk free.

6. Contact Us: info@stjohnslabs.com
1. Choose 5 Trial Size Samples from our free sample list
2. Complete the free sample application: http://www.stjohnslabs.com/free-sample
3. Receive your antibody
4. Submit your review
5. Receive your 30% off your next order
Get Your Free Samples in 5 Easy Steps

7. Follow Us for Weekly Independent
Validation Reviews
@StJohnsLabsSt John's Laboratory Ltd
St John's Laboratory Ltd St John's Laboratory Ltd
http://www.slideshare.net/stjohnslabs