Peptide Dot Blotting Customer Review for Anti-Histone H2B (Di Methyl Lys43) Antibody (STJ97164)

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Histone H2B is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone H2B is a structural protein that helps organise eukaryotic DNA. It plays an important role in the biology of the nucleus where it is involved in the packaging and maintaining of chromosomes, regulation of transcription, and replication and repair of DNA. Histone H2B helps regulate chromatin structure and function through post-translational modifications and specialised histone variants. The antibody detects endogenous Histone H2B (Di Methyl Lys43) protein. Anti-Histone H2B (Di Methyl Lys43) Antibody- http://www.stjohnslabs.com/histone-h2b-di-methyl-lys43-antibody?filter_name=STJ97164 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Result
“Some Specificity detected. However due to low antibody concentration result was inconclusive. Peptide test on the
antibody is only showing weak signal.“ Q . Fang, Western University
Lane No. Antigen Loading amount Primary antibody
Primary antibody
dilution ratio
Secondary
antibody dilution
ratio
Visualiza-
tion time
1
H2BK43me3/2/1
/0 peptide
100 nM
STJ97165
H2BK43me2
antibody
1:1000 1:5000 120S
Materials for Validation: Short histone peptide
Method of validation: Peptide dot blotting
Primary Antibody:
STJ97164 H2BK43me2 anti-
body
Secondary Antibody HRP goat anti-rabbit
Reference antibody: Yenzym antibody
Dilution ratio: 1:1000
Protocol
Treatment of materials: 2ul of 100nM peptide was put onto nitrocellulose membrane
Air Dry: 15 mins.
Blocking: 1X TBST with 5% skimmed milk powder for 1 hr at room temperature.
Membrane wash: 10 minutes with TBS-T
Primary antibody incubation: Primary antibody incubated for 2 hours at room temperature.
Membrane wash: 3x 10 minutes with TBS-T.
Secondary antibody incubation: secondary antibody incubated for 1 hour at room temperature.
Membrane wash: 3x 10 minutes with TBS-T
Membrane incubation: Incubate the membrane with ELC reagent for 2 minutes at room temperature.
Antibody Customer Review:
STJ97164Anti-H2BK43me2 antibody
Antibody Specificity:
Antibody Rating:

Histone H2B is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone H2B is a structural protein that helps organise eukaryotic DNA. It plays an important role in the biology of the nucleus where it is involved in the packaging and maintaining of chromosomes, regulation of transcription, and replication and repair of DNA. Histone H2B helps regulate chromatin structure and function through post-translational modifications and specialised histone variants. The antibody detects endogenous Histone H2B (Tri Methyl Lys43) protein. Anti-Histone H2B (Tri Methyl Lys43) antibody - http://www.stjohnslabs.com/histone-h2b-tri-methyl-lys43-antibody?filter_name=STJ97165 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

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St John's Laboratory Ltd

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Anti-β-Tubulin-http://www.stjohnslabs.com/b-tubulin-antibody-p-98567 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

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Immunofluorescence Antibody Customer Review for Anti-CaMKIIβ/γ Antibody (STJ9...

Western Blot Antibody Customer Review for Anti-ALK Polyclonal Antibody (STJ91...

Immunofluorescence Antibody Validation Report for Anti-Tubulin β Antibody (ST...

KDM5 epigenetic modifiers as a focus for drug discovery

Immunofluorescence Antibody Validation Report for Anti-GFAP Antibody (STJ96961)

Immunofluorescence Antibody Validation Report for Anti-β-Tubulin Antibody (ST...

Immunohistochemistry Antibody Validation Report for Anti-GFAP Antibody (STJ96...

Immunohistochemistry Antibody Validation Report for Anti-β-Tubulin Antibody (...

Similar to Peptide Dot Blotting Customer Review for Anti-Histone H2B (Di Methyl Lys43) Antibody (STJ97164)

Western Blot Antibody Customer Review for ADNP Polyclonal Antibody (STJ91502)

St John's Laboratory Ltd

Western Blot Antibody Customer Review for Anti-PDHA1 Polyclonal Antibody (STJ...

St John's Laboratory Ltd

Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial is an enzyme that in humans is encoded by the PDHA1 gene. The pyruvate dehydrogenase complex is responsible for the oxidative decarboxylation of pyruvate, with the final product being Acetyl CoA. Mutations in the PDHA1 gene have been known to cause one form of pyruvate dehydrogenase deficiency. Pyruvate dehydrogenase deficiency is characterized by the buildup of a chemical called lactic acid in the body and a variety of neurological problems. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Anti-PDHA1 - http://www.stjohnslabs.com/pdha1-antibody-p-93852?filter_name=STJ95006 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Western Blot Antibody Customer Review for Anti-Histone H3 (Tri methyl K79) An...

St John's Laboratory Ltd

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Anti-Histone H3 (Tri methyl K79) - http://www.stjohnslabs.com/histone-h3-tri-methyl-k79-antibody-p-98628?filter_name=STJ96993 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Antibody Customer Review For Tau Polyclonal Antibody (STJ95911)

St John's Laboratory Ltd

Western Blot Antibody Customer Review for Anti-Endoplasmin Polyclonal Antibod...

St John's Laboratory Ltd

Western Blot Antibody Customer Review for Anti-PKM2 Polyclonal Antibody (STJ9...

St John's Laboratory Ltd

Pyruvate kinase isozymes M1/M2 (PKM1/M2), also known as pyruvate kinase muscle isozyme (PKM), pyruvate kinase type K, cytosolic thyroid hormone-binding protein (CTHBP), thyroid hormone-binding protein 1 (THBP1), or opa-interacting protein 3 (OIP3), is an enzyme that in humans is encoded by the PKM2 gene. Is a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It plays a general role in caspase independent cell death of tumor cells. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Anti-PMK2 - http://www.stjohnslabs.com/pkm2-antibody-p-93954?filter_name=Stj95142 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunofluorescence Antibody Customer Review for Anti-CEP290 antibody (STJ92224)

St John's Laboratory Ltd

Centrosomal protein of 290 kDa is a protein that in humans is encoded by the CEP290 gene The gene CEP290 is a centrosomal protein that plays an important role in centrosome and cilia development. This gene is vital in the formation of the primary cilium, a small antenna-like projections of the cell membrane that plays an important role in the photoreceptors at the back of the retina (which detect light and color) and in the kidney, brain, and many other organs of the body. Knocking down levels of the CEP290 gene transcript resulted in dramatic suppression of ciliogenesis in retinal pigment epithelial cells in culture, proving just how important CEP290 is to cilia formation. CEP290 Polyclonal Antibody detects endogenous levels of CEP290 protein. Anti-CEP290 - http://www.stjohnslabs.com/cep290-antibody-p-91648?filter_name=STJ92224 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunofluorescence Antibody Customer Review for Anti-Kv2.1 Polyclonal Antibod...

St John's Laboratory Ltd

The Kv2.1 protein or potassium voltage-gated channel subfamily B member 1 is a voltage gated potassium channel that mediates transmembrane transport of potassium in excitable membranes located in the brain, cardiovascular system and the pancreas. The protein is coded by the KCNB1 gene in humans. The Kv2.1 polyclonal antibody detects endogenous levels of Kv2.1 protein. Anti-Kv2.1 antibody - http://www.stjohnslabs.com/kv21-antibody?filter_name=STJ93873 Join Our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Customer Review For AXIN2 Polyclonal Antibody (STJ26209)

St John's Laboratory Ltd

The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. To purchase this antibody, use the following link: http://www.stjohnslabs.com/axin2-antibody-p-67883?filter_name=STJ26209

In Cell-Western Antibody Customer Review for Anti-Beclin-1 Antibody (STJ97761)

St John's Laboratory Ltd

Beclin-1 is a protein that in humans is encoded by the BECN1 gene. Beclin-1 plays an important role in tumorigenesis, and neurodegeneration, being implicated in the autophagic programmed cell death. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors. Anti-Beclin-1 - http://www.stjohnslabs.com/beclin-1-antibody-2?filter_name=stj97761 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

In Cell-Western Antibody Customer Review for Anti-LC3A Antibody (STJ97755)

St John's Laboratory Ltd

Microtubule-associated proteins 1A/1B light chain 3A is a protein that in humans is encoded by the MAP1LC3A gene Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Anti-LC3A - http://www.stjohnslabs.com/lc3a-antibody?filter_name=STJ97755 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Western Blot Antibody Customer Review for Anti-Peroxin 2 Antibody (STJ95040)

St John's Laboratory Ltd

PEX2 is gene on chromosome 8q21.1 that encodes a peroxin, an integral membrane protein involved in peroxisome biosynthesis and integrity, which assembles membrane vesicles before translocation of matrix proteins. Defects of PEX2 cause peroxisome biogenesis disorder complementation group 5, Zelleger syndrome and a form of Refsum disease. Anti-Peroxin 2 - http://www.stjohnslabs.com/peroxin-2-antibody?filter_name=STJ95040 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Western Blot Antibody Customer Review for Anti-Histone H3 (Acetyl Lys14) Ant...

St John's Laboratory Ltd

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Anti-Histone H3 (Acetyl Lys14) Antibody - http://www.stjohnslabs.com/histone-h3-acetyl-lys14-antibody?filter_name=Stj97144 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-OPG Antibody (STJ94831)

St John's Laboratory Ltd

Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis. Anti-OPG-http://www.stjohnslabs.com/opg-antibody Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

In Cell-Western Antibody Customer Review for Anti-MAP LC3β antibody (STJ97398)

St John's Laboratory Ltd

Microtubule-associated proteins 1A/1B light chain 3A is a protein that in humans is encoded by the MAP1LC3A gene. MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Anti-MAP LC3β - http://www.stjohnslabs.com/map-lc3b-antibody?filter_name=STJ97398 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-PERK Antibody (STJ95...

St John's Laboratory Ltd

Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function. / ATP + a protein = ADP + a phosphoprotein. / Perturbation in protein folding in the endoplasmic reticulum (ER) promotes reversible dissociation from HSPA5/BIP and oligomerization, resulting in transautophosphorylation and kinase activity induction. Anti-PERK-http://www.stjohnslabs.com/perk-antibody Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-Phospho-p53 (T18) An...

St John's Laboratory Ltd

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Anti-Phospho-p53 (T18)-http://www.stjohnslabs.com/phospho-p53-t18-antibody Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Western Blot Antibody Customer Review for Anti-Phospho-ALK (Y1507) Polyclonal...

St John's Laboratory Ltd

Anaplastic lymphoma kinase (ALK) also known as ALK tyrosine kinase receptor or CD246 (cluster of differentiation 246) is an enzyme that in humans is encoded by the ALK gene. ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phospho-ALK (Y1507) Polyclonal Antibody detects endogenous levels of ALK protein only when phosphorylated at Y1507. Anti-Phospho-ALK - http://www.stjohnslabs.com/phospho-alk-y1507-antibody?filter_name=STJ90845 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Western Blot Antibody Customer Review for Anti-p47-phox Antibody (STJ94885)

St John's Laboratory Ltd

Neutrophil cytosol factor 1, also known as p47phox, is a protein that in humans is encoded by the NCF1 gene. The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. p47 is vital to the activation of NADPH oxidase. P47 becomes heavily phosphorylated. Anti-p47-phox - http://www.stjohnslabs.com/p47-phox-antibody-p-93740?filter_name=stj94885 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-Glut1 Antibody (STJ9...

St John's Laboratory Ltd

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