Customer Review For mTOR Polyclonal Antibody (STJ94280)
•Download as PPTX, PDF•
1 like•596 views
The customer review summarizes results from testing St John's Laboratory's mTOR Polyclonal Antibody (STJ94280) via western blot. The antibody detected target bands at the expected size of 288kD in HeLa and Jurkat cell lysates. The protocol details the cell lysis, gel electrophoresis, transfer, blocking, primary and secondary antibody probing, washing, and visualization steps. The customer stated that the testing results provided by the testing laboratory showed the target bands were detected by the antibody in the two cell lines. St John's Laboratory invites customers to test their products for free by applying for trial samples online.
Report
Share
Report
Share
Recommended
Independent Antibody Validation For mCherry Tag Monoclonal Antibody (STJ34373)
Independent Antibody Validation For mCherry Tag Monoclonal Antibody (STJ34373)St John's Laboratory Ltd
St John’s Laboratory suppliers a highly photostable mCherry tag antibody, which is resistant to photobleaching. Independent validation reviews provide feedback on real-experimental use:
https://www.scienceexchange.com/validations/29688
https://www.scienceexchange.com/validations/29733
https://www.scienceexchange.com/validations/29708
To purchase this antibody, use the following link: http://www.stjohnslabs.com/mcherry-tag-antibody?filter_name=STJ34373Customer Review For CAR Polyclonal Antibody (STJ91996)
Component of the epithelial apical junction complex that may function as an homophilic cell adhesion molecule and is essential for tight junction integrity. Also involved in transepithelial migration of leukocytes through adhesive interactions with AMICA1/JAML a transmembrane protein of the plasma membrane of leukocytes. The interaction between both receptors also mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, AMICA1 induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/car-antibody?filter_name=STJ91996
Customer Review For GAPDH Polyclonal Antibody (STJ96417)
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/gapdh-antibody-p-94882?filter_name=STJ96417
Customer Review for Histone H4 (TriMethyl Lys20) Polyclonal Antibody (STJ97154)
Customer Review for Histone H4 (TriMethyl Lys20) Polyclonal Antibody (STJ97154)St John's Laboratory Ltd
Histones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli, including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes. Histone H4 lysine 20 (H4K20) may undergo mono-, di-, or trimethylation, which is catalyzed by the methyltransferase PR-Set7 (Set8 or KMT5a). Methylated H4K20 plays a role in regulating DNA damage responses, mitosis, DNA replication, and gene expression. Trimethylation of H4K20 contributes to gene silencing, and is a mark of the repressive heterochromatin state.
To purchase this antibody use the following link: http://www.stjohnslabs.com/histone-h4-tri-methyl-lys20-antibody?filter_name=STJ97154Customer Review For NFκB-p65 Polyclonal Antibody (STJ94468)
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/nfkb-p65-antibody-p-93367?filter_name=STJ94468
Customer Review For GSK3β Polyclonal Antibody (STJ93447)
GSK3β Polyclonal Antibody is an active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/gsk3b-antibody-p-92568?filter_name=STJ93447
Customer Review for Histone H2A (Acetyl Lys5) Polyclonal Antibody (STJ97171)
Customer Review for Histone H2A (Acetyl Lys5) Polyclonal Antibody (STJ97171)St John's Laboratory Ltd
Acetyl-Histone H2A (Lys5) Antibody detects endogenous levels of histone H2A only when acetylated at lysine 5.
To purchase this antibody use the following link: http://www.stjohnslabs.com/histone-h2a-acetyl-lys5-antibody?filter_name=STJ97171
Customer Review For AXIN2 Polyclonal Antibody (STJ26209)
The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/axin2-antibody-p-67883?filter_name=STJ26209
Recommended
Independent Antibody Validation For mCherry Tag Monoclonal Antibody (STJ34373)
Independent Antibody Validation For mCherry Tag Monoclonal Antibody (STJ34373)St John's Laboratory Ltd
St John’s Laboratory suppliers a highly photostable mCherry tag antibody, which is resistant to photobleaching. Independent validation reviews provide feedback on real-experimental use:
https://www.scienceexchange.com/validations/29688
https://www.scienceexchange.com/validations/29733
https://www.scienceexchange.com/validations/29708
To purchase this antibody, use the following link: http://www.stjohnslabs.com/mcherry-tag-antibody?filter_name=STJ34373Customer Review For CAR Polyclonal Antibody (STJ91996)
Component of the epithelial apical junction complex that may function as an homophilic cell adhesion molecule and is essential for tight junction integrity. Also involved in transepithelial migration of leukocytes through adhesive interactions with AMICA1/JAML a transmembrane protein of the plasma membrane of leukocytes. The interaction between both receptors also mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, AMICA1 induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/car-antibody?filter_name=STJ91996
Customer Review For GAPDH Polyclonal Antibody (STJ96417)
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/gapdh-antibody-p-94882?filter_name=STJ96417
Customer Review for Histone H4 (TriMethyl Lys20) Polyclonal Antibody (STJ97154)
Customer Review for Histone H4 (TriMethyl Lys20) Polyclonal Antibody (STJ97154)St John's Laboratory Ltd
Histones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli, including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes. Histone H4 lysine 20 (H4K20) may undergo mono-, di-, or trimethylation, which is catalyzed by the methyltransferase PR-Set7 (Set8 or KMT5a). Methylated H4K20 plays a role in regulating DNA damage responses, mitosis, DNA replication, and gene expression. Trimethylation of H4K20 contributes to gene silencing, and is a mark of the repressive heterochromatin state.
To purchase this antibody use the following link: http://www.stjohnslabs.com/histone-h4-tri-methyl-lys20-antibody?filter_name=STJ97154Customer Review For NFκB-p65 Polyclonal Antibody (STJ94468)
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/nfkb-p65-antibody-p-93367?filter_name=STJ94468
Customer Review For GSK3β Polyclonal Antibody (STJ93447)
GSK3β Polyclonal Antibody is an active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/gsk3b-antibody-p-92568?filter_name=STJ93447
Customer Review for Histone H2A (Acetyl Lys5) Polyclonal Antibody (STJ97171)
Customer Review for Histone H2A (Acetyl Lys5) Polyclonal Antibody (STJ97171)St John's Laboratory Ltd
Acetyl-Histone H2A (Lys5) Antibody detects endogenous levels of histone H2A only when acetylated at lysine 5.
To purchase this antibody use the following link: http://www.stjohnslabs.com/histone-h2a-acetyl-lys5-antibody?filter_name=STJ97171
Customer Review For AXIN2 Polyclonal Antibody (STJ26209)
The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/axin2-antibody-p-67883?filter_name=STJ26209
Customer Review For Tubulin-beta Polyclonal Antibody - IF&ICC Application (ST...
Customer Review For Tubulin-beta Polyclonal Antibody - IF&ICC Application (ST...St John's Laboratory Ltd
Tubulin is the major constituent of microtubules. It binds two molecules of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/tubulin-beta-antibody?filter_name=STJ31562 Customer Review For Actin β Polyclonal Antibody (STJ91464)
Beta-actin (human gene and protein symbol ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/actin-b-antibody?filter_name=STJ91464
Customer Review For FAAH Polyclonal Antibody (STJ23602)
FAAH antibody degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/faah-antibody-p-65320?filter_name=STJ23602
Customer Review for His-tag Polyclonal Antibody (STJ90106)
A polyhistidine-tag is an amino acid motif in proteins that consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, His6 tag and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/his-tag-antibody-p-95792?filter_name=STJ90106
Customer Review For LPCAT1 Polyclonal Antibody (STJ27005)
LPCAT1 antibody possess both acyltransferase and acetyltransferase activities. Activity is calcium-independent. Mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC). Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively. May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology.
Publication:
http://www.ncbi.nlm.nih.gov/pubmed/16864775
http://www.ncbi.nlm.nih.gov/pubmed/21498505
To purchase this antibody, use the following link: http://www.stjohnslabs.com/lpcat1-antibody-p-68679?filter_name=STJ27005
Customer Review for TriMethyl-Histone H3-K56 Polyclonal Antibody (STJ29403)
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
To purchase this antibody use the following link: http://www.stjohnslabs.com/trimethyl-histone-h3-k56?filter_name=STJ29403
Customer Review For ENT1 Polyclonal Antibody (STJ96396)
Equilibrative nucleoside transporter 1 (ENT1) is a protein that in humans is encoded by the SLC29A1 gene. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene.
This gene is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylmercaptopurine ribonucleoside (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/ent1-antibody?filter_name=STJ96396
Customer Review For EphB4 Polyclonal Antibody (STJ23550)
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/ephb4-antibody-p-65270?filter_name=STJ23550
Customer Review For Tubulin-beta Polyclonal Antibody- IF Application (STJ31562)
Customer Review For Tubulin-beta Polyclonal Antibody- IF Application (STJ31562)St John's Laboratory Ltd
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and maintenance.
To purchase this antibody use the following link: http://www.stjohnslabs.com/tubulin-beta-antibody?filter_name=STJ31562 Customer Review For Alpha-synuclein Polyclonal Antibody (STJ95862)
Alpha-synuclein is involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/synuclein-a-antibody-p-94486?filter_name=STJ95862
Customer Review For ERBB4 Polyclonal Antibody (STJ27886)
Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins and EGF family members and regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. Required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. Required for normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. Required for mammary gland differentiation, induction of milk proteins and lactation. Acts as cell-surface receptor for the neuregulins NRG1, NRG2, NRG3 and NRG4 and the EGF family members BTC, EREG and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Ligand specificity and signaling is modulated by alternative splicing, proteolytic processing, and by the formation of heterodimers with other ERBB family members, thereby creating multiple combinations of intracellular phosphotyrosines that trigger ligand- and context-specific cellular responses. Mediates phosphorylation of SHC1 and activation of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Isoform JM-A CYT-1 and isoform JM-B CYT-1 phosphorylate PIK3R1, leading to the activation of phosphatidylinositol 3-kinase and AKT1 and protect cells against apoptosis. Isoform JM-A CYT-1 and isoform JM-B CYT-1 mediate reorganization of the actin cytoskeleton and promote cell migration in response to NRG1. Isoform JM-A CYT-2 and isoform JM-B CYT-2 lack the phosphotyrosine that mediates interaction with PIK3R1, and hence do not phosphorylate PIK3R1, do not protect cells against apoptosis, and do not promote reorganization of the actin cytoskeleton and cell migration. Proteolytic processing of isoform JM-A CYT-1 and isoform JM-A CYT-2 gives rise to the corresponding soluble intracellular domains (4ICD) that translocate to the nucleus, promote nuclear import of STAT5A, activation of STAT5A, mammary epithelium differentiation, cell proliferation and activation of gene expression. The ERBB4 soluble intracellular domains (4ICD) colocalize with STAT5A at the CSN2 promoter to regulate transcription of milk proteins during lactation. The ERBB4 soluble intracellular domains can also translocate to mitochondria and promote apoptosis.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/erbb4?filter_name=STJ27886
Customer Review For NCKX3 Polyclonal Antibody (STJ94358)
Transports 1 Ca2+ and 1 K+ in exchange for 4 Na+.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/nckx3-antibody?filter_name=STJ94358
Customer Review For EphA2 Polyclonal Antibody (STJ92940)
Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/epha2-antibody-p-96094?filter_name=STJ92940
Customer Review For Cdk2 Polyclonal Antibody (STJ92197)
Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/cdk2-antibody-p-91630?filter_name=STJ92197
Customer Review For PIWIL4 Polyclonal Antibody (STJ25009)
PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells. Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/piwil4-antibody-p-66699?filter_name=STJ25009
Customer Review for HSP90A Polyclonal Antibody (STJ93622)
Heat shock proteins, as a class, are among the most highly expressed cellular proteins across all species. As their name implies, heat shock proteins protect cells when stressed by elevated temperatures. They account for 1–2% of total protein in unstressed cells. However, when cells are heated, the fraction of heat shock proteins increases to 4–6% of cellular proteins.
Hsp90 (heat shock protein 90) is a chaperone protein that assists other proteins to fold properly, stabilizes proteins against heat stress, and aids in protein degradation. It is one of the most common of the heat-related proteins. The "90" comes from the fact that it weighs roughly 90 kiloDaltons. A 90 kDa protein is considered fairly large for a non-fibrous protein.
To purchase this antibody use the following link: http://www.stjohnslabs.com/hsp90a-antibody-p-92714?filter_name=HSP90A
Customer Review For CAMSAP3 Polyclonal Antibody (STJ46575)
Microtubule minus-end binding protein that acts as a regulator of non-centrosomal microtubule dynamics and organization. Specifically required for the biogenesis and the maintenance of zonula adherens by anchoring the minus-end of microtubules to zonula adherens and by recruiting the kinesin KIFC3 to those junctional sites. May regulate the nucleation and the polymerization of microtubules. Indirectly, through the microtubule cytoskeleton, may regulate the organization of cellular organelles including the Golgi and the early endosomes.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/camsap3-antibody-n-term-p-78995?filter_name=STJ46575
Customer Review For Cdc2 Polyclonal Antibody (STJ92154)
Cdc2 Polyclonal Antibody plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/cdc2-antibody-p-91597?filter_name=STJ92154
Antibody Customer Review For PHF19/hPCL3 Polyclonal Antibody (STJ110368)
PHD Finger Protein 19 is coded for by the PHF19 gene (also known as hPCL3). The Polycomb Group (PcG) is a key binding site within the protein that targets trimethylated Histone H3 at Lys-36, where it recruits the PRC2 complex. It is thought to be involved in the transition of embryonic stems cells from an active to repressed state, and research has indicated its overexpression in many types of cancers.
Immunohistochemistry Antibody Validation Report for Anti-GSK3β Antibody (STJ9...
Immunohistochemistry Antibody Validation Report for Anti-GSK3β Antibody (STJ9...St John's Laboratory Ltd
Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase. In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA.
Anti-GSK3β-http://www.stjohnslabs.com/gsk3b-antibody-p-92568
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Immunofluorescence Antibody Validation Report for Anti-Phospho-Akt (S473) Ant...
Immunofluorescence Antibody Validation Report for Anti-Phospho-Akt (S473) Ant...St John's Laboratory Ltd
RAC-alpha serine/threonine-protein kinase is an enzyme that in humans is encoded by the AKT1 gene. This enzyme belongs to the AKT subfamily of serine/threonine kinases that contain SH2 (Src homology 2-like) domains.The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1.
Anti-Phospho-Akt (S473) -http://www.stjohnslabs.com/phospho-akt-s473-antibody
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validationImmunohistochemistry Antibody Validation Report for Anti-Phospho-Akt (S473) A...
Immunohistochemistry Antibody Validation Report for Anti-Phospho-Akt (S473) A...St John's Laboratory Ltd
RAC-alpha serine/threonine-protein kinase is an enzyme that in humans is encoded by the AKT1 gene. This enzyme belongs to the AKT subfamily of serine/threonine kinases that contain SH2 (Src homology 2-like) domains.The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1.
Anti-Phospho-Akt (S473) -http://www.stjohnslabs.com/phospho-akt-s473-antibody
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validationMore Related Content
What's hot
Customer Review For Tubulin-beta Polyclonal Antibody - IF&ICC Application (ST...
Customer Review For Tubulin-beta Polyclonal Antibody - IF&ICC Application (ST...St John's Laboratory Ltd
Tubulin is the major constituent of microtubules. It binds two molecules of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/tubulin-beta-antibody?filter_name=STJ31562 Customer Review For Actin β Polyclonal Antibody (STJ91464)
Beta-actin (human gene and protein symbol ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/actin-b-antibody?filter_name=STJ91464
Customer Review For FAAH Polyclonal Antibody (STJ23602)
FAAH antibody degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/faah-antibody-p-65320?filter_name=STJ23602
Customer Review for His-tag Polyclonal Antibody (STJ90106)
A polyhistidine-tag is an amino acid motif in proteins that consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, His6 tag and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/his-tag-antibody-p-95792?filter_name=STJ90106
Customer Review For LPCAT1 Polyclonal Antibody (STJ27005)
LPCAT1 antibody possess both acyltransferase and acetyltransferase activities. Activity is calcium-independent. Mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC). Displays a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively. May synthesize phosphatidylcholine in pulmonary surfactant, thereby playing a pivotal role in respiratory physiology.
Publication:
http://www.ncbi.nlm.nih.gov/pubmed/16864775
http://www.ncbi.nlm.nih.gov/pubmed/21498505
To purchase this antibody, use the following link: http://www.stjohnslabs.com/lpcat1-antibody-p-68679?filter_name=STJ27005
Customer Review for TriMethyl-Histone H3-K56 Polyclonal Antibody (STJ29403)
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
To purchase this antibody use the following link: http://www.stjohnslabs.com/trimethyl-histone-h3-k56?filter_name=STJ29403
Customer Review For ENT1 Polyclonal Antibody (STJ96396)
Equilibrative nucleoside transporter 1 (ENT1) is a protein that in humans is encoded by the SLC29A1 gene. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene.
This gene is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylmercaptopurine ribonucleoside (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/ent1-antibody?filter_name=STJ96396
Customer Review For EphB4 Polyclonal Antibody (STJ23550)
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/ephb4-antibody-p-65270?filter_name=STJ23550
Customer Review For Tubulin-beta Polyclonal Antibody- IF Application (STJ31562)
Customer Review For Tubulin-beta Polyclonal Antibody- IF Application (STJ31562)St John's Laboratory Ltd
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and maintenance.
To purchase this antibody use the following link: http://www.stjohnslabs.com/tubulin-beta-antibody?filter_name=STJ31562 Customer Review For Alpha-synuclein Polyclonal Antibody (STJ95862)
Alpha-synuclein is involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/synuclein-a-antibody-p-94486?filter_name=STJ95862
Customer Review For ERBB4 Polyclonal Antibody (STJ27886)
Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins and EGF family members and regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. Required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. Required for normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. Required for mammary gland differentiation, induction of milk proteins and lactation. Acts as cell-surface receptor for the neuregulins NRG1, NRG2, NRG3 and NRG4 and the EGF family members BTC, EREG and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Ligand specificity and signaling is modulated by alternative splicing, proteolytic processing, and by the formation of heterodimers with other ERBB family members, thereby creating multiple combinations of intracellular phosphotyrosines that trigger ligand- and context-specific cellular responses. Mediates phosphorylation of SHC1 and activation of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Isoform JM-A CYT-1 and isoform JM-B CYT-1 phosphorylate PIK3R1, leading to the activation of phosphatidylinositol 3-kinase and AKT1 and protect cells against apoptosis. Isoform JM-A CYT-1 and isoform JM-B CYT-1 mediate reorganization of the actin cytoskeleton and promote cell migration in response to NRG1. Isoform JM-A CYT-2 and isoform JM-B CYT-2 lack the phosphotyrosine that mediates interaction with PIK3R1, and hence do not phosphorylate PIK3R1, do not protect cells against apoptosis, and do not promote reorganization of the actin cytoskeleton and cell migration. Proteolytic processing of isoform JM-A CYT-1 and isoform JM-A CYT-2 gives rise to the corresponding soluble intracellular domains (4ICD) that translocate to the nucleus, promote nuclear import of STAT5A, activation of STAT5A, mammary epithelium differentiation, cell proliferation and activation of gene expression. The ERBB4 soluble intracellular domains (4ICD) colocalize with STAT5A at the CSN2 promoter to regulate transcription of milk proteins during lactation. The ERBB4 soluble intracellular domains can also translocate to mitochondria and promote apoptosis.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/erbb4?filter_name=STJ27886
Customer Review For NCKX3 Polyclonal Antibody (STJ94358)
Transports 1 Ca2+ and 1 K+ in exchange for 4 Na+.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/nckx3-antibody?filter_name=STJ94358
Customer Review For EphA2 Polyclonal Antibody (STJ92940)
Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/epha2-antibody-p-96094?filter_name=STJ92940
Customer Review For Cdk2 Polyclonal Antibody (STJ92197)
Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/cdk2-antibody-p-91630?filter_name=STJ92197
Customer Review For PIWIL4 Polyclonal Antibody (STJ25009)
PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells. Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/piwil4-antibody-p-66699?filter_name=STJ25009
Customer Review for HSP90A Polyclonal Antibody (STJ93622)
Heat shock proteins, as a class, are among the most highly expressed cellular proteins across all species. As their name implies, heat shock proteins protect cells when stressed by elevated temperatures. They account for 1–2% of total protein in unstressed cells. However, when cells are heated, the fraction of heat shock proteins increases to 4–6% of cellular proteins.
Hsp90 (heat shock protein 90) is a chaperone protein that assists other proteins to fold properly, stabilizes proteins against heat stress, and aids in protein degradation. It is one of the most common of the heat-related proteins. The "90" comes from the fact that it weighs roughly 90 kiloDaltons. A 90 kDa protein is considered fairly large for a non-fibrous protein.
To purchase this antibody use the following link: http://www.stjohnslabs.com/hsp90a-antibody-p-92714?filter_name=HSP90A
Customer Review For CAMSAP3 Polyclonal Antibody (STJ46575)
Microtubule minus-end binding protein that acts as a regulator of non-centrosomal microtubule dynamics and organization. Specifically required for the biogenesis and the maintenance of zonula adherens by anchoring the minus-end of microtubules to zonula adherens and by recruiting the kinesin KIFC3 to those junctional sites. May regulate the nucleation and the polymerization of microtubules. Indirectly, through the microtubule cytoskeleton, may regulate the organization of cellular organelles including the Golgi and the early endosomes.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/camsap3-antibody-n-term-p-78995?filter_name=STJ46575
Customer Review For Cdc2 Polyclonal Antibody (STJ92154)
Cdc2 Polyclonal Antibody plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins.
To purchase this antibody, use the following link: http://www.stjohnslabs.com/cdc2-antibody-p-91597?filter_name=STJ92154
Antibody Customer Review For PHF19/hPCL3 Polyclonal Antibody (STJ110368)
PHD Finger Protein 19 is coded for by the PHF19 gene (also known as hPCL3). The Polycomb Group (PcG) is a key binding site within the protein that targets trimethylated Histone H3 at Lys-36, where it recruits the PRC2 complex. It is thought to be involved in the transition of embryonic stems cells from an active to repressed state, and research has indicated its overexpression in many types of cancers.
Immunohistochemistry Antibody Validation Report for Anti-GSK3β Antibody (STJ9...
Immunohistochemistry Antibody Validation Report for Anti-GSK3β Antibody (STJ9...St John's Laboratory Ltd
Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase. In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA.
Anti-GSK3β-http://www.stjohnslabs.com/gsk3b-antibody-p-92568
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
What's hot (20)
Customer Review For Tubulin-beta Polyclonal Antibody - IF&ICC Application (ST...
Customer Review For Tubulin-beta Polyclonal Antibody - IF&ICC Application (ST...
Customer Review For Actin β Polyclonal Antibody (STJ91464)
Customer Review For Actin β Polyclonal Antibody (STJ91464)
Customer Review For FAAH Polyclonal Antibody (STJ23602)
Customer Review For FAAH Polyclonal Antibody (STJ23602)
Customer Review for His-tag Polyclonal Antibody (STJ90106)
Customer Review for His-tag Polyclonal Antibody (STJ90106)
Customer Review For LPCAT1 Polyclonal Antibody (STJ27005)
Customer Review For LPCAT1 Polyclonal Antibody (STJ27005)
Customer Review for TriMethyl-Histone H3-K56 Polyclonal Antibody (STJ29403)
Customer Review for TriMethyl-Histone H3-K56 Polyclonal Antibody (STJ29403)
Customer Review For ENT1 Polyclonal Antibody (STJ96396)
Customer Review For ENT1 Polyclonal Antibody (STJ96396)
Customer Review For EphB4 Polyclonal Antibody (STJ23550)
Customer Review For EphB4 Polyclonal Antibody (STJ23550)
Customer Review For Tubulin-beta Polyclonal Antibody- IF Application (STJ31562)
Customer Review For Tubulin-beta Polyclonal Antibody- IF Application (STJ31562)
Customer Review For Alpha-synuclein Polyclonal Antibody (STJ95862)
Customer Review For Alpha-synuclein Polyclonal Antibody (STJ95862)
Customer Review For ERBB4 Polyclonal Antibody (STJ27886)
Customer Review For ERBB4 Polyclonal Antibody (STJ27886)
Customer Review For NCKX3 Polyclonal Antibody (STJ94358)
Customer Review For NCKX3 Polyclonal Antibody (STJ94358)
Customer Review For EphA2 Polyclonal Antibody (STJ92940)
Customer Review For EphA2 Polyclonal Antibody (STJ92940)
Customer Review For Cdk2 Polyclonal Antibody (STJ92197)
Customer Review For Cdk2 Polyclonal Antibody (STJ92197)
Customer Review For PIWIL4 Polyclonal Antibody (STJ25009)
Customer Review For PIWIL4 Polyclonal Antibody (STJ25009)
Customer Review for HSP90A Polyclonal Antibody (STJ93622)
Customer Review for HSP90A Polyclonal Antibody (STJ93622)
Customer Review For CAMSAP3 Polyclonal Antibody (STJ46575)
Customer Review For CAMSAP3 Polyclonal Antibody (STJ46575)
Customer Review For Cdc2 Polyclonal Antibody (STJ92154)
Customer Review For Cdc2 Polyclonal Antibody (STJ92154)
Antibody Customer Review For PHF19/hPCL3 Polyclonal Antibody (STJ110368)
Antibody Customer Review For PHF19/hPCL3 Polyclonal Antibody (STJ110368)
Immunohistochemistry Antibody Validation Report for Anti-GSK3β Antibody (STJ9...
Immunohistochemistry Antibody Validation Report for Anti-GSK3β Antibody (STJ9...
Viewers also liked
Immunofluorescence Antibody Validation Report for Anti-Phospho-Akt (S473) Ant...
Immunofluorescence Antibody Validation Report for Anti-Phospho-Akt (S473) Ant...St John's Laboratory Ltd
RAC-alpha serine/threonine-protein kinase is an enzyme that in humans is encoded by the AKT1 gene. This enzyme belongs to the AKT subfamily of serine/threonine kinases that contain SH2 (Src homology 2-like) domains.The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1.
Anti-Phospho-Akt (S473) -http://www.stjohnslabs.com/phospho-akt-s473-antibody
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validationImmunohistochemistry Antibody Validation Report for Anti-Phospho-Akt (S473) A...
Immunohistochemistry Antibody Validation Report for Anti-Phospho-Akt (S473) A...St John's Laboratory Ltd
RAC-alpha serine/threonine-protein kinase is an enzyme that in humans is encoded by the AKT1 gene. This enzyme belongs to the AKT subfamily of serine/threonine kinases that contain SH2 (Src homology 2-like) domains.The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1.
Anti-Phospho-Akt (S473) -http://www.stjohnslabs.com/phospho-akt-s473-antibody
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validationWestern Blot Antibody Customer Review for Anti-Peroxin 2 Antibody (STJ95040)
Western Blot Antibody Customer Review for Anti-Peroxin 2 Antibody (STJ95040)St John's Laboratory Ltd
PEX2 is gene on chromosome 8q21.1 that encodes a peroxin, an integral membrane protein involved in peroxisome biosynthesis and integrity, which assembles membrane vesicles before translocation of matrix proteins.
Defects of PEX2 cause peroxisome biogenesis disorder complementation group 5, Zelleger syndrome and a form of Refsum disease.
Anti-Peroxin 2 - http://www.stjohnslabs.com/peroxin-2-antibody?filter_name=STJ95040
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validationAntibody Customer Review For p70 S6 Kinase Polyclonal Antibody (STJ31332)
p70 S6 kinase or p70S6K is a serine/threonine kinase that acts downstream of PIP3 and phosphoinositide-dependent kinase-1 in the PI3 kinase pathway. As the name suggests, its target substrate is the S6 ribosomal protein. Phosphorylation of S6 induces protein synthesis at the ribosome.
The phosphorylation of P70S6K at threonine 389 has been used as a hallmark of activation by mTOR and correlated with autophagy inhibition in various situations. However, several recent studies suggest that the activity of P70S6K plays a more positive role in the increase of autophagy.
To purchase this antibody use the following link: http://www.stjohnslabs.com/p70-s6-kinase-antibody?filter_name=STJ31332
Indirect Flow Cytometry Protocol
Indirect flow cytometry, also known as fluorescence-activated cell sorting (FACS) is a specialised type of flow cytometry. It provides a method for sorting a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell. It is a useful scientific instrument as it provides fast, objective and quantitative recording of fluorescent signals from individual cells as well as physical separation of cells of particular interest.
In contrast to direct flow cytometry, FACS requires two incubation steps. Firstly with the appropriate primary antibody, then with a fluorochrome-labelled secondary antibody.
Antibody Customer Review For p70 S6 Kinase (Phospho-Thr389/412) Polyclonal An...
Antibody Customer Review For p70 S6 Kinase (Phospho-Thr389/412) Polyclonal An...St John's Laboratory Ltd
p70S6 kinase or p70S6K is a serine/threonine kinase that acts downstream of PIP3 and phosphoinositide-dependent kinase-1 in the PI3 kinase pathway. As the name suggests, its target substrate is the S6 ribosomal protein. Phosphorylation of S6 induces protein synthesis at the ribosome.
The phosphorylation of P70S6K at threonine 389 has been used as a hallmark of activation by mTOR and correlated with autophagy inhibition in various situations. However, several recent studies suggest that the activity of P70S6K plays a more positive role in the increase of autophagy.
Phosphorylation of Thr229 in the catalytic domain and Thr389 in the linker domain are most critical for kinase function. Phosphorylation of Thr389, however, most closely correlates with p70 kinase activity in vivo. Prior phosphorylation of Thr389
is required for the action of phosphoinositide 3-dependent
protein kinase 1 (PDK1) on Thr229. Phosphorylation
of this site is stimulated by growth factors such as insulin,
EGF and FGF, as well as by serum and some G-proteincoupled receptor ligands, and is blocked by wortmannin, LY294002 (PI-3K inhibitor) and rapamycin (FRAP/mTOR inhibitor) .
To purchase this antibody use the following link: http://www.stjohnslabs.com/p70-s6-kinase-antibody-phospho-thr389412?filter_name=STJ30854Immunocytochemistry Protocol
This method is used to visualise the localisation and quantity of a protein of interest. The target protein is bound to by a specific primary antibody, which in turn is detected by a secondary antibody conjugated to a fluorophore. A fluorescent or confocal microscope is used to visualise the protein.
Immunocytochemistry (ICC) differs from immunohistochemistry (IHC) in that the former is performed on samples of intact cells that have had most, if not all, of their surrounding extracellular matrix removed. In contrast, immunohistochemical samples are sections of biological tissue, where each cell is surrounded by tissue architecture and other cells normally found in the intact tissue. These differences cause the samples to be prepared differently. For ICC, the sample requires permeabilisation so that the antibodies can reach the intracellular targets. Depending on the thickness of the sample, IHC samples do not require this.
Do you have a technical question? Get in touch: info@stjohnslabs.com
Western Blot Protocol - St John's Laboratory
The western blot technique uses gel electrophoresis to separate proteins in a tissue homogenate or extract by molecular weight. The separated proteins on the gel are then transferred to a membrane (usually nitrocellulose or PVDF) which is then incubated with an antibody specific for a target protein. The protein of interest can be visualised using conjugated secondary antibodies and detection reagents.
Do you have a technical question? Get in touch: info@stjohnslabs.com
Protocol series - http://www.stjohnslabs.com/services/video-protocol-series
Immunofluorescence (IF) Protocol
Immunofluorescence or Immunofluorescence Antibody Assay(IFA) is a traditional laboratory technique that utilizes fluorescent dyes to identify the presence of antibodies bound to specific antigens.
Immunofluorescence is a powerful technique which allows for the visualisation of proteins or antigens within a cell or a section of tissue. Utilizing the binding specificity of an antibody to a given antigen, antibodies chemically conjugated with fluorescent dyes can be used to bind to specific molecular targets. This allows for easy visualisation by confocal or fluorescent microscopy.
Immunohistochemistry Antibody Validation Report for Anti-NFκB-p65 (Acetyl K31...
Immunohistochemistry Antibody Validation Report for Anti-NFκB-p65 (Acetyl K31...St John's Laboratory Ltd
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one.
Anti-NFκB-p65 (Acetyl K310)-http://www.stjohnslabs.com/acetyl-nfkb-p65-k310-antibody
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validationSandwich ELISA Protocol
A sandwich ELISA measures the amount of antigen between two layers of antibodies. One layer is the capture antibody, the other is the detection antibody. The antigen to be measured must contain at least two antigenic sites capable of binding to antibody, with one binding to the capture antibody and the other binding to the detection antibody.
Sandwich ELISA is very sensitive (more so than direct or indirect ELISA) and the sample does not have to be purified before analysis.
Immunofluorescence Antibody Validation Report for Anti-p70 S6 kinase α Antibo...
Immunofluorescence Antibody Validation Report for Anti-p70 S6 kinase α Antibo...St John's Laboratory Ltd
Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation.
Anti-p70 S6 kinase α -http://www.stjohnslabs.com/p70-s6-kinase-a-antibody-p-93776
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validationImmunofluorescence Antibody Validation Report for Anti-p21 Antibody (STJ94862)
Immunofluorescence Antibody Validation Report for Anti-p21 Antibody (STJ94862)St John's Laboratory Ltd
May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.
Anti-p21 -http://www.stjohnslabs.com/p21-antibody
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validationIndirect ELISA Protocol | St John's Laboratory
The enzyme-linked immunosorbent assay (ELISA) uses antibodies and a solid-phase enzyme immunoassay to detect the presence of a specific antigen in a liquid or wet sample. Antigens from the sample are immobilised on a solid support either non-specifically or specifically. A specific antibody is then applied over the surface so it can bind to the antigen. This antibody is linked to an enzyme or can itself be detected by an enzyme-linked secondary antibody. In the final step, a substance containing the enzyme's substrate is added. The subsequent reaction produces a visible signal, most commonly a colour change in the substrate, which indicates the quantity of antigen in the sample.
When the presence of an antigen is analysed, the name "direct ELISA" refers to an ELISA in which only a labelled primary antibody is used, whereas the term "indirect ELISA" refers to an ELISA in which the antigen is bound by the primary antibody which then is detected by a labelled secondary antibody.
Immunofluorescence Antibody Validation Report for Anti-p70 S6 kinase α Antibo...
Immunofluorescence Antibody Validation Report for Anti-p70 S6 kinase α Antibo...St John's Laboratory Ltd
Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis.
Anti-p70 S6 kinase α -http://www.stjohnslabs.com/p70-s6-kinase-a-antibody-p-99094
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validationImmunofluorescence Antibody Validation Report for Anti-p70 S6 kinase α Antibo...
Immunofluorescence Antibody Validation Report for Anti-p70 S6 kinase α Antibo...St John's Laboratory Ltd
Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis.
Anti-p70 S6 kinase α -http://www.stjohnslabs.com/p70-s6-kinase-a-antibody-p-99095
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validationViewers also liked (16)
Immunofluorescence Antibody Validation Report for Anti-Phospho-Akt (S473) Ant...
Immunofluorescence Antibody Validation Report for Anti-Phospho-Akt (S473) Ant...
Immunohistochemistry Antibody Validation Report for Anti-Phospho-Akt (S473) A...
Immunohistochemistry Antibody Validation Report for Anti-Phospho-Akt (S473) A...
Western Blot Antibody Customer Review for Anti-Peroxin 2 Antibody (STJ95040)
Western Blot Antibody Customer Review for Anti-Peroxin 2 Antibody (STJ95040)
Antibody Customer Review For p70 S6 Kinase Polyclonal Antibody (STJ31332)
Antibody Customer Review For p70 S6 Kinase Polyclonal Antibody (STJ31332)
Antibody Customer Review For p70 S6 Kinase (Phospho-Thr389/412) Polyclonal An...
Antibody Customer Review For p70 S6 Kinase (Phospho-Thr389/412) Polyclonal An...
Immunohistochemistry Antibody Validation Report for Anti-NFκB-p65 (Acetyl K31...
Immunohistochemistry Antibody Validation Report for Anti-NFκB-p65 (Acetyl K31...
Immunofluorescence Antibody Validation Report for Anti-p70 S6 kinase α Antibo...
Immunofluorescence Antibody Validation Report for Anti-p70 S6 kinase α Antibo...
Immunofluorescence Antibody Validation Report for Anti-p21 Antibody (STJ94862)
Immunofluorescence Antibody Validation Report for Anti-p21 Antibody (STJ94862)
Immunofluorescence Antibody Validation Report for Anti-p70 S6 kinase α Antibo...
Immunofluorescence Antibody Validation Report for Anti-p70 S6 kinase α Antibo...
Immunofluorescence Antibody Validation Report for Anti-p70 S6 kinase α Antibo...
Immunofluorescence Antibody Validation Report for Anti-p70 S6 kinase α Antibo...
Similar to Customer Review For mTOR Polyclonal Antibody (STJ94280)
Antibody Customer Review For FKRP Polyclonal Antibody (STJ93080)
Fukutin-related protein (FKRP) is targeted to the medial Golgi apparatus and is necessary for post-translational modification of dystroglycan. Mutations in the gene encoding this protein have been associated with congenital muscular dystrophy, mental retardation, and cerebellar cysts. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
To purchase this antibody use the following link: http://www.stjohnslabs.com/fkrp-antibody-p-95246?filter_name=FKRP
Antibody Customer Review For HIF-1α Polyclonal Antibody (STJ93498)
Hypoxia-inducible factor 1-alpha, also known as HIF-1α, is a subunit of a heterodimeric transcription factor hypoxia-inducible factor 1 (HIF-1) that is encoded by the HIF1α gene. It is a basic helix-loop-helix PAS domain containing protein, and is considered as the master transcriptional regulator of cellular and developmental response to hypoxia. The dysregulation and overexpression of HIF1α by either hypoxia or genetic alternations have been heavily implicated in cancer biology, as well as a number of other pathophysiologies, specifically in areas of vascularization and angiogenesis, energy metabolism, cell survival and tumour invasion.
To purchase this antibody use the following link: http://www.stjohnslabs.com/hif-1a-antibody-p-92614?filter_name=STJ93498
Antibody Customer Review For Tau Polyclonal Antibody (STJ95911)
Tau are stabilising proteins for axonal microtubules within the Central Nervous System, and as such are also recognised as Mictrotubule-associated Protein (MAP). Tau is predominantly active in the distal portions of axons to carry out its function.
Antibody Customer Review For VAPB Polyclonal Antibody (STJ47066)
Vesicle-associated membrane protein-associated protein B (VAPB) is a type IV membrane protein found in plasma and intracellular vesicle membranes. The protein is found as a homodimer and as a heterodimer with VAPA. It also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking. Like VAPA, VAPB binds to proteins that contain a FFAT motif. Considerable interest in VAPB has arisen because mutations in this protein are associated with rare, familial forms of Motor Neurone Disease (also called Amyotrophic Lateral Sclerosis and Lou Gehrig's disease).
To purchase this antibody use the following link: http://www.stjohnslabs.com/vapb-antibody-c-term-p-79486?filter_name=STJ47066
St John's Laboratory Ltd Booklet 2015
St John's Laboratory Booklet 2015, includes company story, customer reviews, antibody validation project.
Antibody Customer Review For Phospho-eIF2α (S51) Polyclonal Antibody (STJ90253)
Antibody Customer Review For Phospho-eIF2α (S51) Polyclonal Antibody (STJ90253) St John's Laboratory Ltd
eIF2 is an initiation factor found within eukaryotes. It mediates binding of tRNA to the ribosome in a GTP dependent manner. The alpha subunit is the main target for phosphorylation at Serine-51, and is considered the regulatory subunit of this trimeric (formed of eIF2a, eIF2b and eIF2y).
Visit www.stjohnslabs.comWestern Blot Antibody Customer Review for Anti-Phospho-ALK (Y1507) Polyclonal...
Western Blot Antibody Customer Review for Anti-Phospho-ALK (Y1507) Polyclonal...St John's Laboratory Ltd
Anaplastic lymphoma kinase (ALK) also known as ALK tyrosine kinase receptor or CD246 (cluster of differentiation 246) is an enzyme that in humans is encoded by the ALK gene.
ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phospho-ALK (Y1507) Polyclonal Antibody detects endogenous levels of ALK protein only when phosphorylated at Y1507.
Anti-Phospho-ALK - http://www.stjohnslabs.com/phospho-alk-y1507-antibody?filter_name=STJ90845
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Western Blot Antibody Customer Review for p63 Polyclonal Antibody (STJ94912)
Western Blot Antibody Customer Review for p63 Polyclonal Antibody (STJ94912)St John's Laboratory Ltd
TP63 (tumour protein p63) or transformation-related protein 63 acts as a sequence specific DNA binding transcriptional activator or repressor. It is encoded by the TP63 gene in humans. The p63 polyclonal antibody detects endogenous levels of p63 protein.Western Blot Antibody Customer Review for Anti-Bad Antibody (STJ91797)
The Bcl-2-associated death promoter (BAD) protein is a pro-apoptotic member of the Bcl-2 gene family which is involved in initiating apoptosis. BAD is a member of the BH3-only family, a subfamily of the Bcl-2 family. After activation, it is able to form a heterodimer with anti-apoptotic proteins and prevent them from stopping apoptosis.
Anti-Bad - http://www.stjohnslabs.com/bad-antibody-p-91310?filter_name=STJ91797
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Western Blot Antibody Customer Review for Anti-PKM2 Polyclonal Antibody (STJ9...
Western Blot Antibody Customer Review for Anti-PKM2 Polyclonal Antibody (STJ9...St John's Laboratory Ltd
Pyruvate kinase isozymes M1/M2 (PKM1/M2), also known as pyruvate kinase muscle isozyme (PKM), pyruvate kinase type K, cytosolic thyroid hormone-binding protein (CTHBP), thyroid hormone-binding protein 1 (THBP1), or opa-interacting protein 3 (OIP3), is an enzyme that in humans is encoded by the PKM2 gene.
Is a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It plays a general role in caspase independent cell death of tumor cells.
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Anti-PMK2 - http://www.stjohnslabs.com/pkm2-antibody-p-93954?filter_name=Stj95142
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validationWestern Blot Antibody Customer Review for Anti-PDHA1 Polyclonal Antibody (STJ...
Western Blot Antibody Customer Review for Anti-PDHA1 Polyclonal Antibody (STJ...St John's Laboratory Ltd
Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial is an enzyme that in humans is encoded by the PDHA1 gene.
The pyruvate dehydrogenase complex is responsible for the oxidative decarboxylation of pyruvate, with the final product being Acetyl CoA.
Mutations in the PDHA1 gene have been known to cause one form of pyruvate dehydrogenase deficiency. Pyruvate dehydrogenase deficiency is characterized by the buildup of a chemical called lactic acid in the body and a variety of neurological problems.
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Anti-PDHA1 - http://www.stjohnslabs.com/pdha1-antibody-p-93852?filter_name=STJ95006
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Western Blot Customer Review Anti-WAVE2 Antibody (STJ96263)
Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.
Anti-WAVE2 -http://www.stjohnslabs.com/wave2-antibody
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Western Blot Antibody Customer Review for Anti-RelB Polyclonal Antibody (STJ9...
Western Blot Antibody Customer Review for Anti-RelB Polyclonal Antibody (STJ9...St John's Laboratory Ltd
The RelB protein is a transcription factor encoded by the RELB gene in humans. It is known to interact with NFKB2. The RelB polyclonal antibody detects endogenous levels of RelB protein. Western Blot Antibody Customer Review for Anti-Endoplasmin Polyclonal Antibod...
Western Blot Antibody Customer Review for Anti-Endoplasmin Polyclonal Antibod...St John's Laboratory Ltd
The Endoplasmin protein (also known as HSP90B1) is a molecular chaperone encoded by the HSP90B1 gene in humans. It plays a role in the processing and transport of proteins such as integrins and Toll-like receptors. It is located in the endoplasmic reticulum. The Endoplasmin polyclonal antibody detects endogenous levels of Endoplasmin protein.Western Blot Antibody Customer Review for Anti-RUNX1 Polyclonal Antibody (STJ...
Western Blot Antibody Customer Review for Anti-RUNX1 Polyclonal Antibody (STJ...St John's Laboratory Ltd
RUNX1 protein (Runt-related transcription factor 1) also known as AML1 is a transcriptional corepressor encoded by the RUNX1 gene in humans. It regulates the differentiation of hematopoietic stem cells into mature blood cells via its association with DNA-binding transcription factors as well as its recruitment of other corepressors and histone-modifying enzymes. Western Blot Antibody Customer Review for ADNP Polyclonal Antibody (STJ91502)
Western Blot Antibody Customer Review for ADNP Polyclonal Antibody (STJ91502)St John's Laboratory Ltd
ADNP (Activity-Dependent Neuroprotector Homeobox) is expressed through the ADNP gene in humans. It is a possible transcription factor through having a homeobox and 9 zinc finger domains. It is said to have both stimulatory and inhibitory growth effects on different tumour cells. The ADNP Polyclonal Antibody detects endogenous levels of ADNP protein.Western Blot Customer Review Anti-β-tubulin Antibody (STJ97037)
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
Anti-β-tubulin -http://www.stjohnslabs.com/b-tubulin-antibody-p-98672
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Western Blot Antibody Customer Review for Anti-p47-phox Antibody (STJ94885)
Neutrophil cytosol factor 1, also known as p47phox, is a protein that in humans is encoded by the NCF1 gene.
The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. p47 is vital to the activation of NADPH oxidase. P47 becomes heavily phosphorylated.
Anti-p47-phox - http://www.stjohnslabs.com/p47-phox-antibody-p-93740?filter_name=stj94885
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Western Blot Antibody Customer Review for Anti-Histone H3 (Tri methyl K79) An...
Western Blot Antibody Customer Review for Anti-Histone H3 (Tri methyl K79) An...St John's Laboratory Ltd
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.
Anti-Histone H3 (Tri methyl K79) - http://www.stjohnslabs.com/histone-h3-tri-methyl-k79-antibody-p-98628?filter_name=STJ96993
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validationSimilar to Customer Review For mTOR Polyclonal Antibody (STJ94280) (20)
Antibody Customer Review For FKRP Polyclonal Antibody (STJ93080)
Antibody Customer Review For FKRP Polyclonal Antibody (STJ93080)
Antibody Customer Review For HIF-1α Polyclonal Antibody (STJ93498)
Antibody Customer Review For HIF-1α Polyclonal Antibody (STJ93498)
Antibody Customer Review For Tau Polyclonal Antibody (STJ95911)
Antibody Customer Review For Tau Polyclonal Antibody (STJ95911)
Antibody Customer Review For VAPB Polyclonal Antibody (STJ47066)
Antibody Customer Review For VAPB Polyclonal Antibody (STJ47066)
Antibody Customer Review For Phospho-eIF2α (S51) Polyclonal Antibody (STJ90253)
Antibody Customer Review For Phospho-eIF2α (S51) Polyclonal Antibody (STJ90253)
Western Blot Antibody Customer Review for Anti-Phospho-ALK (Y1507) Polyclonal...
Western Blot Antibody Customer Review for Anti-Phospho-ALK (Y1507) Polyclonal...
Western Blot Antibody Customer Review for p63 Polyclonal Antibody (STJ94912)
Western Blot Antibody Customer Review for p63 Polyclonal Antibody (STJ94912)
Western Blot Antibody Customer Review for Anti-Bad Antibody (STJ91797)
Western Blot Antibody Customer Review for Anti-Bad Antibody (STJ91797)
Western Blot Antibody Customer Review for Anti-PKM2 Polyclonal Antibody (STJ9...
Western Blot Antibody Customer Review for Anti-PKM2 Polyclonal Antibody (STJ9...
Western Blot Antibody Customer Review for Anti-PDHA1 Polyclonal Antibody (STJ...
Western Blot Antibody Customer Review for Anti-PDHA1 Polyclonal Antibody (STJ...
Western Blot Customer Review Anti-WAVE2 Antibody (STJ96263)
Western Blot Customer Review Anti-WAVE2 Antibody (STJ96263)
Western Blot Antibody Customer Review for Anti-RelB Polyclonal Antibody (STJ9...
Western Blot Antibody Customer Review for Anti-RelB Polyclonal Antibody (STJ9...
Western Blot Antibody Customer Review for Anti-Endoplasmin Polyclonal Antibod...
Western Blot Antibody Customer Review for Anti-Endoplasmin Polyclonal Antibod...
Western Blot Antibody Customer Review for Anti-RUNX1 Polyclonal Antibody (STJ...
Western Blot Antibody Customer Review for Anti-RUNX1 Polyclonal Antibody (STJ...
Western Blot Antibody Customer Review for ADNP Polyclonal Antibody (STJ91502)
Western Blot Antibody Customer Review for ADNP Polyclonal Antibody (STJ91502)
Western Blot Customer Review Anti-β-tubulin Antibody (STJ97037)
Western Blot Customer Review Anti-β-tubulin Antibody (STJ97037)
Western Blot Antibody Customer Review for Anti-p47-phox Antibody (STJ94885)
Western Blot Antibody Customer Review for Anti-p47-phox Antibody (STJ94885)
Western Blot Antibody Customer Review for Anti-Histone H3 (Tri methyl K79) An...
Western Blot Antibody Customer Review for Anti-Histone H3 (Tri methyl K79) An...
More from St John's Laboratory Ltd
Immunohistochemistry Customer Review Anti-Smo antibody (STJ95710)
G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia.
Anti-Smo antibody (STJ95710): http://www.stjohnslabs.com/smo-antibody-p-94371?filter_name=STJ95710
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Western Blot Customer Review Anti Glucocorticoid Receptor antibody (STJ97101)
Western Blot Customer Review Anti Glucocorticoid Receptor antibody (STJ97101)St John's Laboratory Ltd
Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling . Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity). Has transcriptional activation and repression activity . Mediates glucocorticoid-induced apoptosis . Promotes accurate chromosome segregation during mitosis . May act as a tumor suppressor . May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic gene expression (By similarity). / Isoform Beta: Acts as a dominant negative inhibitor of isoform Alpha . Has intrinsic transcriptional activity independent of isoform Alpha when both isoforms are coexpressed.
Join our Antibody Validation Project: http://www.stjohnslabs.com/services/antibody-validation
Anti glucocorticoid receptor antibody (STJ97101):
http://www.stjohnslabs.com/glucocorticoid-receptor-antibody-p-98736?filter_name=STJ97101Western Blot Customer Review Anti-Phospho-Cofilin (S3) Antibody (STJ90230)
Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation.
Anti-Phospho-Cofilin (S3) -http://www.stjohnslabs.com/phospho-cofilin-s3-antibody
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Scientist of the Month - May Byron Baron
This June, Dr. Byron Baron from the University of Malta, Malta, is our Scientist of the Month! He's shared with us his research highlights, his current projects and some comments on the biotechnology industry.
Want to be our Scientist of the Month? Contact info@stjohnslabs.com
Western Blot Customer Review Anti-Endophilin I Antibody (STJ92924)
Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature.
Brain, mostly in frontal cortex. Expressed at high level in fetal cerebellum.
Anti-Endophilin I -http://www.stjohnslabs.com/endophilin-i-antibody
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Immunohistochemistry Antibody Validation Report for Anti-Caspase-8 Antibody (...
Immunohistochemistry Antibody Validation Report for Anti-Caspase-8 Antibody (...St John's Laboratory Ltd
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. / Strict requirement for Asp at position P1 and has a preferred cleavage sequence of (Leu/Asp/Val)-Glu-Thr-Asp-|-(Gly/Ser/Ala). / Inhibited by the effector protein NleF that is produced by pathogenic E.coli; this inhibits apoptosis.
Anti-Caspase-8-http://www.stjohnslabs.com/caspase-8-antibody-p-99045
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validationImmunohistochemistry Antibody Validation Report for Anti-Gamma Tubulin Antibo...
Immunohistochemistry Antibody Validation Report for Anti-Gamma Tubulin Antibo...St John's Laboratory Ltd
Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.
Anti-Gamma Tubulin-http://www.stjohnslabs.com/gamma-tubulin-antibody
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Immunohistochemistry Antibody Validation Report for Anti-Epsilon Tubulin Anti...
Immunohistochemistry Antibody Validation Report for Anti-Epsilon Tubulin Anti...St John's Laboratory Ltd
Tubulin in molecular biology can refer either to the tubulin protein superfamily of globular proteins, or one of the member proteins of that superfamily. α- and β-tubulins polymerize into microtubules, a major component of the eukaryotic cytoskeleton. Microtubules function in many essential cellular processes, including mitosis. Tubulin-binding drugs kill cancerous cells by inhibiting microtubule dynamics, which are required for DNA segregation and therefore cell division. In eukaryotes there are six members of the tubulin superfamily, although not all are present in all species (see below). Both α and β tubulins have a mass of around 50 kDa and are thus in a similar range compared to actin with ~42 kDa. In contrast, tubulin polymers (microtubules) tend to be much bigger than actin filaments due to their cylindrical nature. Tubulin was long thought to be specific to eukaryotes. More recently, however, several prokaryotic proteins have been shown to be related to tubulin.
Anti-Epsilon Tubulin -http://www.stjohnslabs.com/epsilon-tubulin-antibody-2
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validationImmunohistochemistry Antibody Validation Report for Anti-Luciferase Antibody ...
Immunohistochemistry Antibody Validation Report for Anti-Luciferase Antibody ...St John's Laboratory Ltd
Produces green light with a wavelength of 562 nm. / Photinus luciferin + O2 + ATP = oxidized Photinus luciferin + CO2 + AMP + diphosphate + light.
Anti-Luciferase -http://www.stjohnslabs.com/luciferase-antibody-2
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validationImmunohistochemistry Antibody Validation Report for Anti-LC3A Antibody (STJ97...
Immunohistochemistry Antibody Validation Report for Anti-LC3A Antibody (STJ97...St John's Laboratory Ltd
Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) . Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation .
Anti-LC3A-http://www.stjohnslabs.com/lc3a-antibody
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validationImmunohistochemistry Antibody Validation Report for Anti-CHOP Antibody (STJ97...
Immunohistochemistry Antibody Validation Report for Anti-CHOP Antibody (STJ97...St John's Laboratory Ltd
Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG).
Anti-CHOP-http://www.stjohnslabs.com/chop-antibody-2
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validationImmunohistochemistry Antibody Validation Report for Anti-phospho-MLKL (S358) ...
Immunohistochemistry Antibody Validation Report for Anti-phospho-MLKL (S358) ...St John's Laboratory Ltd
Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity.
Anti-phospho-MLKL (S358)-http://www.stjohnslabs.com/phospho-mlkl-s358-antibody-1
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validationImmunohistochemistry Antibody Validation Report for Anti-ERK1 Antibody (STJ97...
Immunohistochemistry Antibody Validation Report for Anti-ERK1 Antibody (STJ97...St John's Laboratory Ltd
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation.
Anti-ERK1-http://www.stjohnslabs.com/erk1-antibody-3
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
Immunohistochemistry Antibody Validation Report for Anti-PDGFRα Antibody (STJ...
Immunohistochemistry Antibody Validation Report for Anti-PDGFRα Antibody (STJ...St John's Laboratory Ltd
Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation.
Anti-PDGFRα-http://www.stjohnslabs.com/pdgfra-antibody-2
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validationImmunohistochemistry Antibody Validation Report for Anti-Caspase-1 Antibody (...
Immunohistochemistry Antibody Validation Report for Anti-Caspase-1 Antibody (...St John's Laboratory Ltd
Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. / Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|-. / Specifically inhibited by the cowpox virus Crma protein.
Anti-Caspase-1-http://www.stjohnslabs.com/caspase-1-antibody-1
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validationImmunohistochemistry Antibody Validation Report for Anti-Amyloid-β Antibody (...
Immunohistochemistry Antibody Validation Report for Anti-Amyloid-β Antibody (...St John's Laboratory Ltd
Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity (By similarity). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu2+-mediated low-density lipoprotein oxidation.
Anti-Amyloid-β-http://www.stjohnslabs.com/amyloid-v-antibody
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validationImmunohistochemistry Antibody Validation Report for Anti-Bcl-2 Antibody (STJ9...
Immunohistochemistry Antibody Validation Report for Anti-Bcl-2 Antibody (STJ9...St John's Laboratory Ltd
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release .
Anti-Bcl-2-http://www.stjohnslabs.com/bcl-2-antibody-1
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validationImmunohistochemistry Antibody Validation Report for Anti-α-SMA Antibody (STJ9...
Immunohistochemistry Antibody Validation Report for Anti-α-SMA Antibody (STJ9...St John's Laboratory Ltd
Alpha-actin-2 also known as actin, aortic smooth muscle or alpha smooth muscle actin (α-SMA, SMactin, alpha-SM-actin, ASMA) is a protein that in humans is encoded by the ACTA2 gene located on 10q22-q24. Actin alpha 2, the human aortic smooth muscle actin gene, is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. Alpha-smooth muscle actin (α-SMA) is commonly used as a marker of myofibroblast formation.
Anti-α-SMA -http://www.stjohnslabs.com/a-sma-antibody-1
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validationImmunohistochemistry Antibody Validation Report for Anti-E2F-1 Antibody (STJ9...
Immunohistochemistry Antibody Validation Report for Anti-E2F-1 Antibody (STJ9...St John's Laboratory Ltd
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters . / BIRC2/c-IAP1 stimulates its transcriptional activity.
Anti-E2F-1-http://www.stjohnslabs.com/e2f-1-antibody-1
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validationImmunohistochemistry Antibody Validation Report for Anti-Annexin I Antibody (...
Immunohistochemistry Antibody Validation Report for Anti-Annexin I Antibody (...St John's Laboratory Ltd
Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity. Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (By similarity). Promotes resolution of inflammation and wound healing. Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades. Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors.
Anti-Annexin I - http://www.stjohnslabs.com/anti-annexin-i-antibody?filter_name=STJ98699
Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation
More from St John's Laboratory Ltd (20)
Immunohistochemistry Customer Review Anti-Smo antibody (STJ95710)
Immunohistochemistry Customer Review Anti-Smo antibody (STJ95710)
Western Blot Customer Review Anti Glucocorticoid Receptor antibody (STJ97101)
Western Blot Customer Review Anti Glucocorticoid Receptor antibody (STJ97101)
Western Blot Customer Review Anti-Phospho-Cofilin (S3) Antibody (STJ90230)
Western Blot Customer Review Anti-Phospho-Cofilin (S3) Antibody (STJ90230)
Western Blot Customer Review Anti-Endophilin I Antibody (STJ92924)
Western Blot Customer Review Anti-Endophilin I Antibody (STJ92924)
Immunohistochemistry Antibody Validation Report for Anti-Caspase-8 Antibody (...
Immunohistochemistry Antibody Validation Report for Anti-Caspase-8 Antibody (...
Immunohistochemistry Antibody Validation Report for Anti-Gamma Tubulin Antibo...
Immunohistochemistry Antibody Validation Report for Anti-Gamma Tubulin Antibo...
Immunohistochemistry Antibody Validation Report for Anti-Epsilon Tubulin Anti...
Immunohistochemistry Antibody Validation Report for Anti-Epsilon Tubulin Anti...
Immunohistochemistry Antibody Validation Report for Anti-Luciferase Antibody ...
Immunohistochemistry Antibody Validation Report for Anti-Luciferase Antibody ...
Immunohistochemistry Antibody Validation Report for Anti-LC3A Antibody (STJ97...
Immunohistochemistry Antibody Validation Report for Anti-LC3A Antibody (STJ97...
Immunohistochemistry Antibody Validation Report for Anti-CHOP Antibody (STJ97...
Immunohistochemistry Antibody Validation Report for Anti-CHOP Antibody (STJ97...
Immunohistochemistry Antibody Validation Report for Anti-phospho-MLKL (S358) ...
Immunohistochemistry Antibody Validation Report for Anti-phospho-MLKL (S358) ...
Immunohistochemistry Antibody Validation Report for Anti-ERK1 Antibody (STJ97...
Immunohistochemistry Antibody Validation Report for Anti-ERK1 Antibody (STJ97...
Immunohistochemistry Antibody Validation Report for Anti-PDGFRα Antibody (STJ...
Immunohistochemistry Antibody Validation Report for Anti-PDGFRα Antibody (STJ...
Immunohistochemistry Antibody Validation Report for Anti-Caspase-1 Antibody (...
Immunohistochemistry Antibody Validation Report for Anti-Caspase-1 Antibody (...
Immunohistochemistry Antibody Validation Report for Anti-Amyloid-β Antibody (...
Immunohistochemistry Antibody Validation Report for Anti-Amyloid-β Antibody (...
Immunohistochemistry Antibody Validation Report for Anti-Bcl-2 Antibody (STJ9...
Immunohistochemistry Antibody Validation Report for Anti-Bcl-2 Antibody (STJ9...
Immunohistochemistry Antibody Validation Report for Anti-α-SMA Antibody (STJ9...
Immunohistochemistry Antibody Validation Report for Anti-α-SMA Antibody (STJ9...
Immunohistochemistry Antibody Validation Report for Anti-E2F-1 Antibody (STJ9...
Immunohistochemistry Antibody Validation Report for Anti-E2F-1 Antibody (STJ9...
Immunohistochemistry Antibody Validation Report for Anti-Annexin I Antibody (...
Immunohistochemistry Antibody Validation Report for Anti-Annexin I Antibody (...
Recently uploaded
RNA INTERFERENCE: UNRAVELING GENETIC SILENCING
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Astronomy Update- Curiosity’s exploration of Mars _ Local Briefs _ leadertele...
Article written for leader telegram
Nutraceutical market, scope and growth: Herbal drug technology
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
extra-chromosomal-inheritance[1].pptx.pdfpdf
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Structural Classification Of Protein (SCOP)
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
The ASGCT Annual Meeting was packed with exciting progress in the field advan...
The ASGCT Annual Meeting was packed with exciting progress in the field advancing efforts to deliver highly promising therapies to more patients.
Seminar of U.V. Spectroscopy by SAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Predicting property prices with machine learning algorithms.pdf
This is a report about predicting property prices using SVM, random forest and gradient boosting machine
insect morphology and physiology of insect
the documents provide complete information of insect morphology and their structure
Multi-source connectivity as the driver of solar wind variability in the heli...
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Recently uploaded (20)
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...
Astronomy Update- Curiosity’s exploration of Mars _ Local Briefs _ leadertele...
Astronomy Update- Curiosity’s exploration of Mars _ Local Briefs _ leadertele...
Nutraceutical market, scope and growth: Herbal drug technology
Nutraceutical market, scope and growth: Herbal drug technology
The ASGCT Annual Meeting was packed with exciting progress in the field advan...
The ASGCT Annual Meeting was packed with exciting progress in the field advan...
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...
Body fluids_tonicity_dehydration_hypovolemia_hypervolemia.pptx
Body fluids_tonicity_dehydration_hypovolemia_hypervolemia.pptx
Predicting property prices with machine learning algorithms.pdf
Predicting property prices with machine learning algorithms.pdf
In silico drugs analogue design: novobiocin analogues.pptx
In silico drugs analogue design: novobiocin analogues.pptx
Multi-source connectivity as the driver of solar wind variability in the heli...
Multi-source connectivity as the driver of solar wind variability in the heli...
PRESENTATION ABOUT PRINCIPLE OF COSMATIC EVALUATION
PRESENTATION ABOUT PRINCIPLE OF COSMATIC EVALUATION
Customer Review For mTOR Polyclonal Antibody (STJ94280)
- 1. HOW DID OUR ANTIBODY PERFORM? CUSTOMER REVIEW: mTOR Polyclonal Antibody (STJ94280)
- 2. WHAT WAS USED? Primary antibody: STJ94280. mTOR Polyclonal Antibody. 100µl Provider: St John's Laboratory Dilution ratio: 1:1000 Application: Western Blot. Image: In HeLa and Jurkat cell line, there were target bands detected by STJ94280 mTOR Polyclonal Antibody.
- 3. WHAT WAS THE PROTOCOL? Treatment of materials: When cells were cultured to 70%-80% plating density, lysis buffer with protease inhibitors (Sigma) and equal volume of 2X loading buffer were added for 10 min water bath at 100˚C. Gel electrophoresis: 10% polyacrylamide gel, constant voltage 160 V, 60 min. Transfer: 0.45 nm PVDF membrane, 1 piece of gel (wet transfer), constant voltage 100 V 60 min. Blocking: 1X TBST with 5% skimmed milk powder for 2 hr. Primary antibody probing: primary antibody was added to the solution of 1X TBST with 5% skimmed milk powder, 4˚C for overnight. Membrane wash: 1X TBST wash for 3 times. Secondary antibody probing: secondary antibody was added to the solution of 1X TBST with 5% skimmed milk powder with 1:10000 dilution ratio, for 1.5 hr. Membrane wash: 1X TBST wash for 3 times. Visualization: ECL visualization for 5 min (the visualization time was determined by actual result. It was ensured that the visualization time was identical for the same antigen).
- 4. WHAT WAS THE PROTOCOL? Gel electrophoresis information：10% polyacrylamide gel, constant voltage 160 V, 60 min. Transfer information：0.45 nm PVDF membrane, 1 piece of gel (wet transfer), constant voltage 100 V 60 min. No. Antigen Loading amount Primary antibody Primary antibody dilution ratio Secondary antibody dilution ratio Target band KD Visualization time 1 HeLa lysate 10 µg STJ94280. mTOR Polyclonal Antibody 1:1000 1:10000 288KD 35S 2 Jurkat lysate 10 µg 1:10000 288KD 35S
- 5. WHAT DID THE CUSTOMER THINK? Testing results were provided by BPI laboratory. They stated that “In HeLa and Jurkat cell line, there were target bands detected by STJ94280 mTOR Polyclonal Antibody”.
- 6. “WE INVITE YOU TO TEST OUR PRODUCTS” Our aim is to not only connect our customers with the best product, but to also give them the opportunity to test the product’s performance risk free.
- 7. Contact Us: info@stjohnslabs.com 1. Choose 5 Trial Size Samples from our free sample list 2. Complete the free sample application: http://www.stjohnslabs.com/free-sample 3. Receive your antibody 4. Submit your review 5. Receive your 30% off your next order Get Your Free Samples in 5 Easy Steps
- 8. Follow Us for Weekly Independent Validation Reviews @StJohnsLabsSt John's Laboratory Ltd St John's Laboratory Ltd St John's Laboratory Ltd http://www.slideshare.net/stjohnslabs