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Customer Review for Histone H2A (Acetyl Lys5) Polyclonal Antibody (STJ97171)

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Acetyl-Histone H2A (Lys5) Antibody detects endogenous levels of histone H2A only when acetylated at lysine 5.

To purchase this antibody use the following link: http://www.stjohnslabs.com/histone-h2a-acetyl-lys5-antibody?filter_name=STJ97171

Published in: Science
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Customer Review for Histone H2A (Acetyl Lys5) Polyclonal Antibody (STJ97171)

  1. 1. HOW DID OUR ANTIBODY PERFORM? CUSTOMER REVIEW: Histone H2A (Acetyl Lys5) Polyclonal Antibody (STJ97171)
  2. 2. WHAT WAS USED? Primary antibody: STJ97171. Histone H2A (Acetyl Lys5) Polyclonal Antibody. Provider: St John's Laboratory Dilution ratio: 1:250 Application: Immunofluorescence Materials for Validation: Drosophila 3rd instar imaginal discs
  3. 3. WHAT WAS THE PROTOCOL? Fixation: 4% paraformaldehyde in PBS + 0.1% Triton-X for 5 minutes. Blocking: 5% FBS in PBS + 0.4% Triton-X for 45 minutes. Primary antibody probing: primary antibody was added to the solution of blocking buffer, 4°C overnight. Wash: PBS + 0.4% Triton-X wash for 3 times. Secondary antibody probing: secondary antibody (Alexa- conjugated goat anti-rabbit 568) was added to the blocking buffer with 1:500 dilution ratio, for 2 hrs. Wash: 1X PBS + 0.4% Triton-X wash for 3 times. Mounting: DAPI-staining conducted in PBS for 10 minutes, followed by mounting with Prolong Gold Antifade. Visualization: Deltavision fluorescence microscope.
  4. 4. WHAT WAS THE PROTOCOL? Fixation: 4% paraformaldehyde in PBS + 0.1% Triton-X for 5 minutes. Blocking: 5% FBS in PBS + 0.4% Triton-X for 30 minutes. No. Antigen Primary antibody Primary antibody dilution ratio Secondary antibody dilution ratio Co-staining Antibody Mounting Medium 1 Diploid imaginal disc cells STJ97171. Histone H2A (Acetyl Lys5) Polyclonal Antibody 1:250 1:500 DAPI Prolong Gold
  5. 5. WHAT DID THE CUSTOMER THINK? Testing results were provided by Serafin Colmenares from Lawrence Berkeley National Laboratory. They stated that “H2AK5Ac signal overlaps with complete nucleus, except for DAPI bright heterochromatic region. This is in line with the expected signal for this specific modification.”
  6. 6. “WE INVITE YOU TO TEST OUR PRODUCTS” Our aim is to not only connect our customers with the best product, but to also give them the opportunity to test the product’s performance risk free.
  7. 7. Contact Us: info@stjohnslabs.com 1. Choose 5 Trial Size Samples from our free sample list 2. Complete the free sample application: http://www.stjohnslabs.com/free-sample 3. Receive your antibody 4. Submit your review 5. Receive your 30% off your next order Get Your Free Samples in 5 Easy Steps
  8. 8. Follow Us for Weekly Independent Validation Reviews @StJohnsLabsSt John's Laboratory Ltd St John's Laboratory Ltd St John's Laboratory Ltd http://www.slideshare.net/stjohnslabs

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