Western Blot Customer Review Anti-WAVE2 Antibody (STJ96263)

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Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Anti-WAVE2 -http://www.stjohnslabs.com/wave2-antibody Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

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ANTIBODY CUSTOMER REVIEW
Findings: “Major band is at the right molecular
weight. Lower and Upper unspecific bands.” –
Loic Fort, Beatson Institute for Cancer Research
Knowledge Dock Business Centre, University Way, London E16 2RD
T: (+44) 0208 223 3081 F: (+44) 0207 681 2580 E: info@stjohnslabs.com w: www.stjohnslabs.com
Protocol
Gel
Electrophoresis
4-12% polyacrylamide gel, 1x
MOPS buffer, constant voltage
180 V, 60 min.
Membrane
Wash
1X TBST wash 3 times 10
minutes.
Transfer 0.45 nm nitrocellulose
membrane, 1 piece of gel (wet
transfer), constant voltage
110V 60 min.
Secondary
Antibody
Probing
Secondary antibody in 1X TBST
with 5% fatty free milk, 1:10000
dilution ratio, for 60 minutes.
Blocking 1X TBST with 5% BSA for 1 hr. Membrane
Wash
1X TBST wash 3 times 10
minutes.
Primary
Antibody
Probing
Primary antibody in 1X TBST
with 5% BSA, 4 ̊C overnight.
Visualisation Li-Cor Odyssey scanner at 680
and 800nm
Model Number STJ96263
Antibody Name Anti-WAVE2 antibody
Application Western Blot
Species Mouse
Tissue B16F1
Dilution 1:500
Secondary Antibody Goat anti-Rabbit IgG
(H&L) Secondary
Antibody DyLight 800
Secondary Dilution 1:10000
Reference antibody Mouse anti alpha
Tubulin DM1A (Sigma),
Goat anti Mouse
AlexaFluor 680nm
secondary antibody
(LifeTechnology)

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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. To purchase this antibody use the following link: http://www.stjohnslabs.com/trimethyl-histone-h3-k56?filter_name=STJ29403

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Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins and EGF family members and regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. Required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. Required for normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. Required for mammary gland differentiation, induction of milk proteins and lactation. Acts as cell-surface receptor for the neuregulins NRG1, NRG2, NRG3 and NRG4 and the EGF family members BTC, EREG and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Ligand specificity and signaling is modulated by alternative splicing, proteolytic processing, and by the formation of heterodimers with other ERBB family members, thereby creating multiple combinations of intracellular phosphotyrosines that trigger ligand- and context-specific cellular responses. Mediates phosphorylation of SHC1 and activation of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Isoform JM-A CYT-1 and isoform JM-B CYT-1 phosphorylate PIK3R1, leading to the activation of phosphatidylinositol 3-kinase and AKT1 and protect cells against apoptosis. Isoform JM-A CYT-1 and isoform JM-B CYT-1 mediate reorganization of the actin cytoskeleton and promote cell migration in response to NRG1. Isoform JM-A CYT-2 and isoform JM-B CYT-2 lack the phosphotyrosine that mediates interaction with PIK3R1, and hence do not phosphorylate PIK3R1, do not protect cells against apoptosis, and do not promote reorganization of the actin cytoskeleton and cell migration. Proteolytic processing of isoform JM-A CYT-1 and isoform JM-A CYT-2 gives rise to the corresponding soluble intracellular domains (4ICD) that translocate to the nucleus, promote nuclear import of STAT5A, activation of STAT5A, mammary epithelium differentiation, cell proliferation and activation of gene expression. The ERBB4 soluble intracellular domains (4ICD) colocalize with STAT5A at the CSN2 promoter to regulate transcription of milk proteins during lactation. The ERBB4 soluble intracellular domains can also translocate to mitochondria and promote apoptosis. To purchase this antibody, use the following link: http://www.stjohnslabs.com/erbb4?filter_name=STJ27886

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Vesicle-associated membrane protein-associated protein B (VAPB) is a type IV membrane protein found in plasma and intracellular vesicle membranes. The protein is found as a homodimer and as a heterodimer with VAPA. It also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking. Like VAPA, VAPB binds to proteins that contain a FFAT motif. Considerable interest in VAPB has arisen because mutations in this protein are associated with rare, familial forms of Motor Neurone Disease (also called Amyotrophic Lateral Sclerosis and Lou Gehrig's disease). To purchase this antibody use the following link: http://www.stjohnslabs.com/vapb-antibody-c-term-p-79486?filter_name=STJ47066

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Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling . Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity). Has transcriptional activation and repression activity . Mediates glucocorticoid-induced apoptosis . Promotes accurate chromosome segregation during mitosis . May act as a tumor suppressor . May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic gene expression (By similarity). / Isoform Beta: Acts as a dominant negative inhibitor of isoform Alpha . Has intrinsic transcriptional activity independent of isoform Alpha when both isoforms are coexpressed. Join our Antibody Validation Project: http://www.stjohnslabs.com/services/antibody-validation Anti glucocorticoid receptor antibody (STJ97101): http://www.stjohnslabs.com/glucocorticoid-receptor-antibody-p-98736?filter_name=STJ97101

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PEX2 is gene on chromosome 8q21.1 that encodes a peroxin, an integral membrane protein involved in peroxisome biosynthesis and integrity, which assembles membrane vesicles before translocation of matrix proteins. Defects of PEX2 cause peroxisome biogenesis disorder complementation group 5, Zelleger syndrome and a form of Refsum disease. Anti-Peroxin 2 - http://www.stjohnslabs.com/peroxin-2-antibody?filter_name=STJ95040 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

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Beta-actin (human gene and protein symbol ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. To purchase this antibody, use the following link: http://www.stjohnslabs.com/actin-b-antibody?filter_name=STJ91464

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Equilibrative nucleoside transporter 1 (ENT1) is a protein that in humans is encoded by the SLC29A1 gene. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. This gene is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylmercaptopurine ribonucleoside (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. To purchase this antibody, use the following link: http://www.stjohnslabs.com/ent1-antibody?filter_name=STJ96396

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Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. To purchase this antibody, use the following link: http://www.stjohnslabs.com/gapdh-antibody-p-94882?filter_name=STJ96417

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Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. To purchase this antibody, use the following link: http://www.stjohnslabs.com/cdk2-antibody-p-91630?filter_name=STJ92197

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St John's Laboratory Ltd

Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity. Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (By similarity). Promotes resolution of inflammation and wound healing. Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades. Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors. Anti-Annexin I - http://www.stjohnslabs.com/anti-annexin-i-antibody?filter_name=STJ98699 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-Annexin I Antibody (...

St John's Laboratory Ltd

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. Anti-Tau- http://www.stjohnslabs.com/anti-tau-antibody?filter_name=STJ98827 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-Tau Antibody (STJ98827)

St John's Laboratory Ltd

Transthyretin (TTR) is a transport protein in the serum and cerebrospinal fluid that carries the thyroid hormone thyroxine (T4) and retinol-binding protein bound to retinol. This is how transthyretin gained its name: transports thyroxine and retinol. The liver secretes transthyretin into the blood, and the choroid plexus secretes TTR into the cerebrospinal fluid. TTR was originally called prealbumin(or thyroxine-binding prealbumin) because it ran faster than albumin on electrophoresis gels. Anti-TTR - http://www.stjohnslabs.com/anti-ttr-antibody?filter_name=STJ98876 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-TTR Antibody (STJ98876)

St John's Laboratory Ltd

Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. Anti-HP-1γ-http://www.stjohnslabs.com/anti-hp-1g-antibody?filter_name=STJ98892 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-HP-1γ Antibody (STJ9...

St John's Laboratory Ltd

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