Cdc2 Polyclonal Antibody plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. To purchase this antibody, use the following link: http://www.stjohnslabs.com/cdc2-antibody-p-91597?filter_name=STJ92154

1. HOW DID OUR
ANTIBODY PERFORM?
CUSTOMER REVIEW: Cdc2 Polyclonal Antibody (STJ92154)

2. WHAT WAS USED?
Primary antibody: STJ92154. Cdc2 Polyclonal
Antibody. 100µl
Provider: St John's Laboratory
Dilution ratio: 1:1000
Application: Western Blot.
Image: In HeLa and Jurkat cell lines, there were
target bands detected by STJ92154 Cdc2 Polyclonal
Antibody.

3. WHAT WAS THE PROTOCOL?
Treatment of materials: When cells were cultured to 70%-80% plating density,
lysis buffer with protease inhibitors (Sigma) and equal volume of 2X loading buffer
were added for 10 min water bath at 100˚C.
Gel electrophoresis: 10% polyacrylamide gel, constant voltage 160 V, 60 min.
Transfer: 0.45 nm PVDF membrane, 1 piece of gel (wet transfer), constant voltage
100 V 60 min.
Blocking: 1X TBST with 5% skimmed milk powder for 2 hr.
Primary antibody probing: primary antibody was added to the solution of 1X
TBST with 5% skimmed milk powder, 4˚C for overnight.
Membrane wash: 1X TBST wash for 3 times.
Secondary antibody probing: secondary antibody was added to the solution of 1X
TBST with 5% skimmed milk powder with 1：10000 dilution ratio, for 1.5 hr.
Membrane wash: 1X TBST wash for 3 times.
Visualization: ECL visualization for 5 min (the visualization time was determined
by actual result. It was ensured that the visualization time was identical for the same
antigen).

4. WHAT WAS THE PROTOCOL?
Gel electrophoresis information：10% polyacrylamide gel, constant voltage 160 V, 60 min.
Transfer information：0.45 nm PVDF membrane, 1 piece of gel (wet transfer), constant voltage 100 V 60
min.
No. Antigen
Loading
amount
Primary
antibody
Primary
antibody
dilution
ratio
Secondary
antibody
dilution ratio
Target band
KD
Visualization
time
1
HeLa
lysate
10 µg
STJ92154.
Cdc2
Polyclonal
Antibody
1:1000
1:10000 34KD 13S
2
Jurkat
lysate
10 µg 1:10000 34KD 13S

5. WHAT DID THE CUSTOMER THINK?
Testing results were provided by BPI Laboratory. They
stated that “In HeLa and Jurkat cell line, there were target
bands detected by STJ92154 Cdc2 Polyclonal Antibody”.

6. “WE INVITE YOU TO TEST OUR PRODUCTS”
Our aim is to not only connect our customers
with the best product, but to also give them
the opportunity to test the product’s
performance risk free.

7. Contact Us: info@stjohnslabs.com
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