The Kv2.1 protein or potassium voltage-gated channel subfamily B member 1 is a voltage gated potassium channel that mediates transmembrane transport of potassium in excitable membranes located in the brain, cardiovascular system and the pancreas. The protein is coded by the KCNB1 gene in humans. The Kv2.1 polyclonal antibody detects endogenous levels of Kv2.1 protein. Anti-Kv2.1 antibody - http://www.stjohnslabs.com/kv21-antibody?filter_name=STJ93873 Join Our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

1. Result
“A signal was detected using STJ93873 Kv2.1 as distinct puncta which may corroborate the localization of Kv2.1. The
detected signal was reduced with increasing dilution which may point to good specificity.” - P. Brites , Instituto de
Biologia Molecular e Celular-IBMC.
Cell Line:
In vitro cultured primary hip-
pocampal neurons
Method of validation: Immunofluorescence
Primary Antibody: STJ93873 Anti-Kv2.1 antibody
Secondary Antibody: Donkey anti-rabbit AF568
Dilution ratio: 1:250, 1:1000
Treatment of materials: At DIV10 Cells were fixed with 2% PFA
Protocol
 Neurons were fixed with 2%PFA
 Cells were washed 3 times for 5 minutes with PBS
 Cells were permeabilised with 0.2% triton X100 for 5 minutes at RT
 Cells were washed 3 times for 5 minutes with PBS
 Blocking occured with 0.5% fish gelatin in PBS for 1 hour at 37ºC
 The primary antibody was incubated overnight at 4ºC and then diluted to 1:250 or 1:1000 in 0.5% fish gelatin in PBS
 Cells were washed 3 times for 5 minutes with PBS
 The secondary antibody was incubated at RT for 1 hour and then diluted to 1:1000 in 0.5% fish gelatin in PBS
 Cells were washed 3 times for 5 minutes with PBS
 Cells were mounted in fluoroshield with DAPI moulding medium
Antibody Customer Review:
STJ93873 Anti-Kv2.1 antibody
Antibody Rating:
1:250 dilution
1:1000 dilution
Antibody Specificity: