Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling . Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity). Has transcriptional activation and repression activity . Mediates glucocorticoid-induced apoptosis . Promotes accurate chromosome segregation during mitosis . May act as a tumor suppressor . May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic gene expression (By similarity). / Isoform Beta: Acts as a dominant negative inhibitor of isoform Alpha . Has intrinsic transcriptional activity independent of isoform Alpha when both isoforms are coexpressed. Join our Antibody Validation Project: http://www.stjohnslabs.com/services/antibody-validation Anti glucocorticoid receptor antibody (STJ97101): http://www.stjohnslabs.com/glucocorticoid-receptor-antibody-p-98736?filter_name=STJ97101

1. Rating:
Specificity:
ANTIBODY CUSTOMER REVIEW
Findings: “Half sized band showed up as well, which has
been previous reported by Expression and Distribution of
Glucocorticoid Receptors in the Ovine Fetal Adrenal Cortex: Effect
of Long-term Hypoxia,” - External Lab
Knowledge Dock Business Centre, University Way, London E16 2RD
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Protocol
Gel
Electrophoresis
170mV for 50 min. Membrane
Wash
TBS-Tween (0.05%)
Transfer 350mA for 60 min Secondary
Antibody
Probing
Anti-rabbit IgG, HRP-linked
Antibody, 30 min room
temperature, 1:3000.
Blocking 1 hour 5% non-fat milk in TBS. Membrane
Wash
TBS-Tween (0.05%)
Primary
Antibody
Probing
Primary antibody, dilution with
1:1000, incubate overnight.
Visualisation ECL visualization was undertaken
after 5 sec, 10 sec, 1 min, 5min,
20 min and 1hr.
Model Number STJ97101
Antibody Name Anti-Glucocorticoid
receptor antibody
Application Western Blot
Species Cow
Tissue Brain
Dilution 1:1000
Secondary Antibody Anti-rabbit IgG, HRP-
linked Antibody
Secondary antibody
dilution
1:3000
Fig 1.
The blot that has a band is GR. The two
samples are the same. Ponceau staining was
done as well, but it will not let me upload this.