Centrosomal protein of 290 kDa is a protein that in humans is encoded by the CEP290 gene The gene CEP290 is a centrosomal protein that plays an important role in centrosome and cilia development. This gene is vital in the formation of the primary cilium, a small antenna-like projections of the cell membrane that plays an important role in the photoreceptors at the back of the retina (which detect light and color) and in the kidney, brain, and many other organs of the body. Knocking down levels of the CEP290 gene transcript resulted in dramatic suppression of ciliogenesis in retinal pigment epithelial cells in culture, proving just how important CEP290 is to cilia formation. CEP290 Polyclonal Antibody detects endogenous levels of CEP290 protein. Anti-CEP290 - http://www.stjohnslabs.com/cep290-antibody-p-91648?filter_name=STJ92224 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

1. Result
“This antibody stained basal bodies in the developing mouse olfactory epithelium as expected” - Cecilia Grestner,
University of Utah
Cell Line:
Mouse fresh olfactory
epethelium cryosection
Method of validation: Immunofluorescence (IF)
Primary Antibody:
STJ92224 Anti-CEP290 anti-
body
Secondary Antibody
Alexa488-conjugated Goat-
anti-Rabbit IgG
Dilution ratio: 1:300
Protocol
Treatment of materials:
Blocking: 2% BSA.
Primary antibody probing: 1:300 dilution ratio, incubated overnight at 4C.
Secondary antibody probing: 1:1000 dilution ration, incubated 1 hour at room temperature.
Antibody Customer Review:
STJ92224 Anti-CEP290 antibody
Antibody Specificity:
Antibody Rating:
Figure 1: Green signal is the stained basal bodies of olfactory sensory neurons
in the developing mouse olfactory epithelium .