Microtubule-associated proteins 1A/1B light chain 3A is a protein that in humans is encoded by the MAP1LC3A gene Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Anti-LC3A - http://www.stjohnslabs.com/lc3a-antibody?filter_name=STJ97755 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

1. Result
“I have used them in LI-COR IN CELL WESTERN method. They worked nicely. The method was according to the in-
structions provided with the LI-COR kit with their buffers and antibodies.” - S. Morshed, Icahn School of Medicine at
Mount Sinai and Bronx.
Gel electrophoresis information：N/A
Transfer information：N/A
Lane No. Antigen Loading amount Primary antibody
Primary antibody
dilution ratio
Secondary
antibody dilution
ratio
Exposure
time
Control-Ab Thyrocytes 50,000 cells/well
STJ97755 Anti-
LC3A Antibody
1:100 1:800 10Min
Cell Line: Rat Thyrocytes
Method of validation: LI-COR CELL Western
Primary Antibody: STJ97755 Anti-LC3A Antibody
Secondary Antibody LI-COR Antibody
Dilution ratio: 1:100
Protocol
Treatment of materials: Rat thyrocytes were treated with TSH receptor antibody for 24 hrs.
Cell fixing: 3.7% formaldehyde in 1xPBS, for 20 minutes at room temperature.
Washing: 1XPBS with 0.1% Triton X-100 for 5 minutes per wash. Repeat 4 times.
Blocking: LI-COR Blocking buffer, 1.5 hours at room temperature with moderate shaking.
Primary antibody probing: Overnight at 4°C.
Washing: 1XPBS with 0.1% Tween 20 for 5 minutes at room temperature.
Secondary antibody probing: LI-COR Antibody for 1 hour.
Washing: 1XPBS with 0.1% Tween 20 for 5 minutes at room temperature. Repeat 5 times.
Visualization: Analysed by LI-COR imaging 10 minutes.
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STJ97755 Anti-LC3A Antibody
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Fig.1 - Rat thyrocytes were treated with TSH re-
ceptor antibody for 24 hrs. Fixed, permeabilized
and then stained with primary antibodies. After
washing FAR red conjugated secondary antibody
was used and then analyzed by LI-COR imaging.