FAAH antibody degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates. To purchase this antibody, use the following link: http://www.stjohnslabs.com/faah-antibody-p-65320?filter_name=STJ23602

1. HOW DID OUR
ANTIBODY PERFORM?
CUSTOMER REVIEW: FAAH Polyclonal Antibody (STJ23602)

2. WHAT WAS USED?
Primary antibody:
Product Catalog No: STJ23602. FAAH antibody.
20µl
Provider: St John's Laboratory
Dilution ratio: 1:50
Application: Immunofluorescence.
Image: Dorsal root ganglia stained with FAAH
antibody.

3. WHAT WAS THE PROTOCOL?
1. Samples were frozen and stored at -80°C until sections were cut at 12 μm thick and collected
onto gelatin-coated slides (Menzel GmbH, Braunschweig, Germany).
2. For immunofluorescence labelling, serial sections of DRG were hydrated in PB buffer (pH=7.4)
for 15 mins.
3. Cell membranes were permeabilised in PB containing 0.1% Triton X-100 and afterwards
incubated for 1 h in 10% normal goat serum (NGS, Jackson ImmunoResearch Laboratories,
West Grove, USA) in PB containing 0.4% Triton X-100 (blocking solution).
4. Sections were incubated for 48 hrs at 4°C in a humid chamber with the respective primary
antibodies (FAAH, St. John’s laboratory; 1:50).
5. After three washes in PB, triple immunofluorescence was revealed by incubation at 4°C in for
24 hrs in the dark with the appropriate fluorochrome-conjugated secondary antibody.
6. Sections were washed 3 times for 15 mins with PB and coverslipped with Aquatex mounting
medium (Merck, Darmstadt, Germany).
7. The sections processed for immunofluorescence were studied with a fluorescence microscope
(DM RXA2) with confocal scanner (TCS SL) (Leica Microsystems GmbH, Manheim,
Germany) equipped with the following lasers: Ar 488, He-Ne 543, He-Ne 633.

4. WHAT DID THE CUSTOMER THINK?
Testing results were provided by Natalia Malek from the
Institute of Pharmacology Polish Academy of Sciences.
They stated that “We completed evaluation of FAAH
antibody. It worked really well, so we decided to purchase
a vial from St John’s Laboratory.”

5. “WE INVITE YOU TO TEST OUR PRODUCTS”
Our aim is to not only connect our customers
with the best product, but to also give them
the opportunity to test the product’s
performance risk free.

6. Contact Us: info@stjohnslabs.com
1. Choose 5 Trial Size Samples from our free sample list
2. Complete the free sample application: http://www.stjohnslabs.com/free-sample
3. Receive your antibody
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