Western Blot Antibody Customer Review for Anti-Histone H3 (Acetyl Lys14) Antibody (STJ97144)

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Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Anti-Histone H3 (Acetyl Lys14) Antibody - http://www.stjohnslabs.com/histone-h3-acetyl-lys14-antibody?filter_name=Stj97144 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

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Gel electrophoresis information：10% SDS-PAGE, constant voltage 170V for 60 min
Transfer information：0.45uM nitrocellulose membrane, wet transfer at 250 mA for 60 min.
Lane No. Antigen
Loading
amount
Primary an-
tibody
Primary
antibody
dilution
ratio
Secondary
antibody dilu-
tion ratio
Target band KD
Visuali-
zation
time
1 MCF7 lysate 60 ug
Histone H3
(Acetyle
Lys14) anti-
body
1:500 1:2500 ~15KD
ECL 7
min
Cell Line: MCF7 cell line
Method of validation: Western Blot
Primary Antibody:
Histone H3 (Acetyle Lys14)
antibody
Secondary Antibody Goat anti rabbit IgG HRP
Dilution ratio: 1:500
Protocol
Treatment of materials: Cells were cultured to 80-90% confluency, and lysed in RIPA buffer.
E.g. Gel electrophoresis: 10% SDS-PAGE, constant voltage 170V for 60 min
Transfer: 0.45uM nitrocellulose membrane, wet transfer at 250 mA for 60 min.
Blocking: 5% milk in TBS for 15 min at RT.
Primary antibody probing: 1/500 diluted in 5% milk in TBS, incubated at 4 C overnight.
Membrane wash: TBS wash 3 times, 5 min/each
Secondary antibody probing: 1/2500 diluted in 5% milk in TBS, incubated at RT for 1 h.
Membrane wash: TBS wash 3 times, 5 min/each
Visualization: ECL 7 min
Antibody Customer Review:
STJ97144 Histone H3 (Acetyle Lys14) antibody
Antibody Specificity:
Antibody Rating:

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Component of the epithelial apical junction complex that may function as an homophilic cell adhesion molecule and is essential for tight junction integrity. Also involved in transepithelial migration of leukocytes through adhesive interactions with AMICA1/JAML a transmembrane protein of the plasma membrane of leukocytes. The interaction between both receptors also mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, AMICA1 induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair. To purchase this antibody, use the following link: http://www.stjohnslabs.com/car-antibody?filter_name=STJ91996

Customer Review For ENT1 Polyclonal Antibody (STJ96396)

St John's Laboratory Ltd

Equilibrative nucleoside transporter 1 (ENT1) is a protein that in humans is encoded by the SLC29A1 gene. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. This gene is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylmercaptopurine ribonucleoside (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. To purchase this antibody, use the following link: http://www.stjohnslabs.com/ent1-antibody?filter_name=STJ96396

Customer Review for TriMethyl-Histone H3-K56 Polyclonal Antibody (STJ29403)

St John's Laboratory Ltd

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. To purchase this antibody use the following link: http://www.stjohnslabs.com/trimethyl-histone-h3-k56?filter_name=STJ29403

Bulletin 6376 (2)Shpour Dahaz

Customer Review For ERBB4 Polyclonal Antibody (STJ27886)

St John's Laboratory Ltd

Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins and EGF family members and regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. Required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. Required for normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. Required for mammary gland differentiation, induction of milk proteins and lactation. Acts as cell-surface receptor for the neuregulins NRG1, NRG2, NRG3 and NRG4 and the EGF family members BTC, EREG and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Ligand specificity and signaling is modulated by alternative splicing, proteolytic processing, and by the formation of heterodimers with other ERBB family members, thereby creating multiple combinations of intracellular phosphotyrosines that trigger ligand- and context-specific cellular responses. Mediates phosphorylation of SHC1 and activation of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Isoform JM-A CYT-1 and isoform JM-B CYT-1 phosphorylate PIK3R1, leading to the activation of phosphatidylinositol 3-kinase and AKT1 and protect cells against apoptosis. Isoform JM-A CYT-1 and isoform JM-B CYT-1 mediate reorganization of the actin cytoskeleton and promote cell migration in response to NRG1. Isoform JM-A CYT-2 and isoform JM-B CYT-2 lack the phosphotyrosine that mediates interaction with PIK3R1, and hence do not phosphorylate PIK3R1, do not protect cells against apoptosis, and do not promote reorganization of the actin cytoskeleton and cell migration. Proteolytic processing of isoform JM-A CYT-1 and isoform JM-A CYT-2 gives rise to the corresponding soluble intracellular domains (4ICD) that translocate to the nucleus, promote nuclear import of STAT5A, activation of STAT5A, mammary epithelium differentiation, cell proliferation and activation of gene expression. The ERBB4 soluble intracellular domains (4ICD) colocalize with STAT5A at the CSN2 promoter to regulate transcription of milk proteins during lactation. The ERBB4 soluble intracellular domains can also translocate to mitochondria and promote apoptosis. To purchase this antibody, use the following link: http://www.stjohnslabs.com/erbb4?filter_name=STJ27886

Western Blot Antibody Customer Review for Anti-Histone H3 (Tri methyl K79) An...

St John's Laboratory Ltd

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Anti-Histone H3 (Tri methyl K79) - http://www.stjohnslabs.com/histone-h3-tri-methyl-k79-antibody-p-98628?filter_name=STJ96993 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Customer Review For mTOR Polyclonal Antibody (STJ94280)

St John's Laboratory Ltd

Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E). Moreover, phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, eukaryotic translation initiation factor EIF4B, and the inhibitor of translation initiation PDCD4. Stimulates the pyrimidine biosynthesis pathway, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, and delayed regulation, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), an allosteric activator of CAD at a later step in synthesis, this function is dependent on the mTORC1 complex. Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor. To purchase this antibody, use the following link: http://www.stjohnslabs.com/mtor-antibody-p-93228?filter_name=STJ94280

In Cell-Western Antibody Customer Review for Anti-LC3A Antibody (STJ97755)

St John's Laboratory Ltd

Microtubule-associated proteins 1A/1B light chain 3A is a protein that in humans is encoded by the MAP1LC3A gene Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Anti-LC3A - http://www.stjohnslabs.com/lc3a-antibody?filter_name=STJ97755 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Western Blot Antibody Customer Review for Anti-p47-phox Antibody (STJ94885)

St John's Laboratory Ltd

Neutrophil cytosol factor 1, also known as p47phox, is a protein that in humans is encoded by the NCF1 gene. The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. p47 is vital to the activation of NADPH oxidase. P47 becomes heavily phosphorylated. Anti-p47-phox - http://www.stjohnslabs.com/p47-phox-antibody-p-93740?filter_name=stj94885 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

In Cell-Western Antibody Customer Review for Anti-MAP LC3β antibody (STJ97398)

St John's Laboratory Ltd

Microtubule-associated proteins 1A/1B light chain 3A is a protein that in humans is encoded by the MAP1LC3A gene. MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Anti-MAP LC3β - http://www.stjohnslabs.com/map-lc3b-antibody?filter_name=STJ97398 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Peptide Dot Blotting Customer Review for Anti-Histone H2B (Tri Methyl Lys43) ...

St John's Laboratory Ltd

Histone H2B is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone H2B is a structural protein that helps organise eukaryotic DNA. It plays an important role in the biology of the nucleus where it is involved in the packaging and maintaining of chromosomes, regulation of transcription, and replication and repair of DNA. Histone H2B helps regulate chromatin structure and function through post-translational modifications and specialised histone variants. The antibody detects endogenous Histone H2B (Tri Methyl Lys43) protein. Anti-Histone H2B (Tri Methyl Lys43) antibody - http://www.stjohnslabs.com/histone-h2b-tri-methyl-lys43-antibody?filter_name=STJ97165 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

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Customer Review for Histone H4 (TriMethyl Lys20) Polyclonal Antibody (STJ97154)

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Customer Review for TriMethyl-Histone H3-K56 Polyclonal Antibody (STJ29403)

Bulletin 6376 (2)

Customer Review For ERBB4 Polyclonal Antibody (STJ27886)

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In Cell-Western Antibody Customer Review for Anti-LC3A Antibody (STJ97755)

Western Blot Antibody Customer Review for Anti-p47-phox Antibody (STJ94885)

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Immunohistochemistry Customer Review Anti-Smo antibody (STJ95710)

St John's Laboratory Ltd

G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia. Anti-Smo antibody (STJ95710): http://www.stjohnslabs.com/smo-antibody-p-94371?filter_name=STJ95710 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Western Blot Customer Review Anti Glucocorticoid Receptor antibody (STJ97101)

St John's Laboratory Ltd

Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling . Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (By similarity). Has transcriptional activation and repression activity . Mediates glucocorticoid-induced apoptosis . Promotes accurate chromosome segregation during mitosis . May act as a tumor suppressor . May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic gene expression (By similarity). / Isoform Beta: Acts as a dominant negative inhibitor of isoform Alpha . Has intrinsic transcriptional activity independent of isoform Alpha when both isoforms are coexpressed. Join our Antibody Validation Project: http://www.stjohnslabs.com/services/antibody-validation Anti glucocorticoid receptor antibody (STJ97101): http://www.stjohnslabs.com/glucocorticoid-receptor-antibody-p-98736?filter_name=STJ97101

Western Blot Customer Review Anti-Phospho-Cofilin (S3) Antibody (STJ90230)

St John's Laboratory Ltd

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Anti-Phospho-Cofilin (S3) -http://www.stjohnslabs.com/phospho-cofilin-s3-antibody Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Scientist of the Month - May Byron Baron

St John's Laboratory Ltd

Western Blot Customer Review Anti-WAVE2 Antibody (STJ96263)

St John's Laboratory Ltd

Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Anti-WAVE2 -http://www.stjohnslabs.com/wave2-antibody Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Western Blot Customer Review Anti-Endophilin I Antibody (STJ92924)

St John's Laboratory Ltd

Western Blot Customer Review Anti-β-tubulin Antibody (STJ97037)

St John's Laboratory Ltd

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Anti-β-tubulin -http://www.stjohnslabs.com/b-tubulin-antibody-p-98672 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-Caspase-8 Antibody (...

St John's Laboratory Ltd

Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. / Strict requirement for Asp at position P1 and has a preferred cleavage sequence of (Leu/Asp/Val)-Glu-Thr-Asp-|-(Gly/Ser/Ala). / Inhibited by the effector protein NleF that is produced by pathogenic E.coli; this inhibits apoptosis. Anti-Caspase-8-http://www.stjohnslabs.com/caspase-8-antibody-p-99045 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-Gamma Tubulin Antibo...

St John's Laboratory Ltd

Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation. Anti-Gamma Tubulin-http://www.stjohnslabs.com/gamma-tubulin-antibody Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-Epsilon Tubulin Anti...

St John's Laboratory Ltd

Tubulin in molecular biology can refer either to the tubulin protein superfamily of globular proteins, or one of the member proteins of that superfamily. α- and β-tubulins polymerize into microtubules, a major component of the eukaryotic cytoskeleton. Microtubules function in many essential cellular processes, including mitosis. Tubulin-binding drugs kill cancerous cells by inhibiting microtubule dynamics, which are required for DNA segregation and therefore cell division. In eukaryotes there are six members of the tubulin superfamily, although not all are present in all species (see below). Both α and β tubulins have a mass of around 50 kDa and are thus in a similar range compared to actin with ~42 kDa. In contrast, tubulin polymers (microtubules) tend to be much bigger than actin filaments due to their cylindrical nature. Tubulin was long thought to be specific to eukaryotes. More recently, however, several prokaryotic proteins have been shown to be related to tubulin. Anti-Epsilon Tubulin -http://www.stjohnslabs.com/epsilon-tubulin-antibody-2 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-Luciferase Antibody ...

St John's Laboratory Ltd

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St John's Laboratory Ltd

Immunohistochemistry Antibody Validation Report for Anti-CHOP Antibody (STJ97...

St John's Laboratory Ltd

Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Anti-CHOP-http://www.stjohnslabs.com/chop-antibody-2 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-phospho-MLKL (S358) ...

St John's Laboratory Ltd

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity. Anti-phospho-MLKL (S358)-http://www.stjohnslabs.com/phospho-mlkl-s358-antibody-1 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-ERK1 Antibody (STJ97...

St John's Laboratory Ltd

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. Anti-ERK1-http://www.stjohnslabs.com/erk1-antibody-3 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-PDGFRα Antibody (STJ...

St John's Laboratory Ltd

Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Anti-PDGFRα-http://www.stjohnslabs.com/pdgfra-antibody-2 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-Caspase-1 Antibody (...

St John's Laboratory Ltd

Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. / Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|-. / Specifically inhibited by the cowpox virus Crma protein. Anti-Caspase-1-http://www.stjohnslabs.com/caspase-1-antibody-1 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-Amyloid-β Antibody (...

St John's Laboratory Ltd

Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity (By similarity). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu2+-mediated low-density lipoprotein oxidation. Anti-Amyloid-β-http://www.stjohnslabs.com/amyloid-v-antibody Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-Bcl-2 Antibody (STJ9...

St John's Laboratory Ltd

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release . Anti-Bcl-2-http://www.stjohnslabs.com/bcl-2-antibody-1 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

Immunohistochemistry Antibody Validation Report for Anti-α-SMA Antibody (STJ9...

St John's Laboratory Ltd

Alpha-actin-2 also known as actin, aortic smooth muscle or alpha smooth muscle actin (α-SMA, SMactin, alpha-SM-actin, ASMA) is a protein that in humans is encoded by the ACTA2 gene located on 10q22-q24. Actin alpha 2, the human aortic smooth muscle actin gene, is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. Alpha-smooth muscle actin (α-SMA) is commonly used as a marker of myofibroblast formation. Anti-α-SMA -http://www.stjohnslabs.com/a-sma-antibody-1 Join our Antibody Validation Project - http://www.stjohnslabs.com/services/antibody-validation

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