The customer review summarized that when the Cdk2 Polyclonal Antibody (STJ92197) from St John's Laboratory was used at a 1:1000 dilution in a Western Blot assay of HeLa and Jurkat cell lysates, target bands for Cdk2 were detected at the expected molecular weight of 32kD. The protocol details that cells were lysed and samples prepared using standard methods before being run on a 10% polyacrylamide gel and transferred to a PVDF membrane. Primary and secondary antibody incubations were performed followed by ECL detection, with the expected target bands visualized within 60 seconds. The customer was satisfied with the antibody's performance in detecting the target protein.

1. HOW DID OUR
ANTIBODY PERFORM?
CUSTOMER REVIEW: Cdk2 Polyclonal Antibody (STJ92197)

2. WHAT WAS USED?
Primary antibody: STJ92197. Cdk2 Polyclonal
Antibody. 100µl
Provider: St John's Laboratory
Dilution ratio: 1:1000
Application: Western Blot.
Image: In HeLa and Jurkat cell line, there were target
bands detected by STJ92197 Cdk2 Polyclonal
Antibody.

3. WHAT WAS THE PROTOCOL?
Treatment of materials: When cells were cultured to 70%-80% plating density,
lysis buffer with protease inhibitors (Sigma) and equal volume of 2X loading buffer
were added for 10 min water bath at 100˚C.
Gel electrophoresis: 10% polyacrylamide gel, constant voltage 160 V, 60 min.
Transfer: 0.45 nm PVDF membrane, 1 piece of gel (wet transfer), constant voltage
100 V 60 min.
Blocking: 1X TBST with 5% skimmed milk powder for 2 hr.
Primary antibody probing: primary antibody was added to the solution of 1X
TBST with 5% skimmed milk powder, 4˚C for overnight.
Membrane wash: 1X TBST wash for 3 times.
Secondary antibody probing: secondary antibody was added to the solution of 1X
TBST with 5% skimmed milk powder with 1:10000 dilution ratio, for 1.5 hr.
Membrane wash: 1X TBST wash for 3 times.
Visualization: ECL visualization for 5 min (the visualization time was determined
by actual result. It was ensured that the visualization time was identical for the same
antigen).

4. WHAT WAS THE PROTOCOL?
Gel electrophoresis infromation：10% polyacrylamide gel, constant voltage 160 V, 60 min.
Transfer information：0.45 nm PVDF membrane, 1 piece of gel (wet transfer), constant voltage 100 V 60
min.
No. Antigen
Loading
amount
Primary
antibody
Primary
antibody
dilution
ratio
Secondary
antibody
dilution
ratio
Target band
KD
Visualization
time
1
HeLa
lysate
10 µg St J92197.
Cdk2
Polyclonal
Antibody
1:1000
1:10000 32KD 60S
2
Jurkat
lysate
10 µg 1:10000 32KD 60S

5. WHAT DID THE CUSTOMER THINK?
BPI stated that when “In HeLa and Jurkat cell line, there
were target bands detected by STJ92197 Cdk2 Polyclonal
Antibody”.

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with the best product, but to also give them
the opportunity to test the product’s
performance risk free.

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