The common feature of spectroscopic measure-
ments is that they all measure some spectroscopic properties that are related to the composition and
structure of biochemical species in the sample of interest. There are several types of spectroscopic
measurements: absorption, scattering (elastic and inelastic), and emission. A typical spectroscopic
experiment that allows us to analyze complex biological systems is conceptually simple. Light at a
certain wavelength λ (or frequency ν = c/λ) is used to irradiate a sample of interest. This process is called excitation. Properties of the light that then emerges from the sample are measured and ana-
lyzed. Some properties deal with the fraction of the incident radiation absorbed by the sample: the
techniques involved are collectively called absorption spectroscopy (e.g., ultraviolet [UV], visible, and
infrared (IR) absorption techniques). Other properties are related to the incident radiation reflected
back from the samples (elastic scattering [ES] techniques). Alternatively, one can measure the light
emitted or scattered by the sample, involving processes that occur at wavelengths different from the
excitation wavelength; the techniques involved are fluorescence, phosphorescence, and inelastic scat-
tering (Raman scattering). Other specialized techniques can be used to detect specific properties of the
emitted light, such as its degree of polarization and decay times.
This document discusses types of lasers and their applications. It begins with an introduction to lasers and their basic requirements. It then describes different laser types including solid-state, semiconductor, dye, gas and excimer lasers. The document outlines various scientific applications such as laser spectroscopy, metrology and cooling. It also discusses commercial uses in cutting, welding, printing and displays. Medical laser applications include cosmetic surgery, dentistry and imaging. Finally, military laser applications are described for countermeasures, guidance and targeting.
Biomedical Instrumentation introduction, BioamplifiersPoornima D
This document provides an introduction to medical instrumentation and bioamplifiers. It discusses how medical instrumentation measures and monitors physiological signals in the body using sensors. The key components of a biomedical instrumentation system are described including the measurand, sensor/transducer, signal conditioner, display, and data storage. It then focuses on bioamplifiers, explaining the types (differential, operational, instrumentation, isolation), their characteristics, and how they are used to amplify weak biopotential signals from the body while maintaining signal integrity.
This document discusses semiconductor optical amplifiers (SOAs). It explains that SOAs use stimulated emission to amplify optical signals, like lasers, but have anti-reflection coatings on the facets so light passes through only once. The main types are traveling-wave amplifiers, which are widely used because they amplify signals with a single pass and have a large bandwidth. SOAs have a core made of InGaAsP for gain and InP cladding layers. External pumping by current injection provides carriers that undergo stimulated emission to amplify optical signals. Amplifier gain increases with length and current but saturates with increasing optical power due to depletion of excited carriers.
A Bioamplifier is an electrophysiological device, a variation of the instrumentation amplifier, used to gather and increase the signal integrity of physiologic electrical activity for output to various sources. It may be an independent unit, or integrated into the electrodes.
The document discusses lasers, including:
- LASER is an acronym for Light Amplification by Stimulated Emission of Radiation.
- Lasers were invented in 1958 and are based on Einstein's idea of particle-wave duality of light.
- The key principles of lasers are stimulated emission within an amplifying medium and population inversion within an optical resonator.
- Common laser types discussed include ruby, He-Ne, argon ion, CO2, excimer, and solid-state lasers like Nd:YAG.
This document discusses different types of filters. It describes high-pass filters, which pass high frequencies and block low frequencies. It also describes low-pass filters, which do the opposite by passing low frequencies and blocking high frequencies. The document provides examples of passive and active low-pass filter circuits and discusses their applications in areas like telephone lines, acoustics, and radio transmitters. The objective is to study the characteristics of passive low-pass filters and measure their cut-off frequency.
The document discusses biopotential electrodes and microelectrodes. Biopotential electrodes measure bioelectric potentials through the metal-electrolyte interface between the electrode and body tissues. Microelectrodes are very small electrodes that can penetrate individual cells to obtain intracellular readings. There are two main types of microelectrodes: metal microelectrodes made of thin wires coated with insulation, and micropipette electrodes made of glass pipettes filled with electrolytes compatible with cell fluids. Both types allow precise measurement of electric potentials within cells.
Graphic record heart sound - Phonogram.
Recording the sounds connected with the pumping action of heart.
Sound from heart – phonocardiogram
Instrument to measure this – phonocardiograph
Basic function – to pick up the different heart sound,filter the required and display.
This document discusses types of lasers and their applications. It begins with an introduction to lasers and their basic requirements. It then describes different laser types including solid-state, semiconductor, dye, gas and excimer lasers. The document outlines various scientific applications such as laser spectroscopy, metrology and cooling. It also discusses commercial uses in cutting, welding, printing and displays. Medical laser applications include cosmetic surgery, dentistry and imaging. Finally, military laser applications are described for countermeasures, guidance and targeting.
Biomedical Instrumentation introduction, BioamplifiersPoornima D
This document provides an introduction to medical instrumentation and bioamplifiers. It discusses how medical instrumentation measures and monitors physiological signals in the body using sensors. The key components of a biomedical instrumentation system are described including the measurand, sensor/transducer, signal conditioner, display, and data storage. It then focuses on bioamplifiers, explaining the types (differential, operational, instrumentation, isolation), their characteristics, and how they are used to amplify weak biopotential signals from the body while maintaining signal integrity.
This document discusses semiconductor optical amplifiers (SOAs). It explains that SOAs use stimulated emission to amplify optical signals, like lasers, but have anti-reflection coatings on the facets so light passes through only once. The main types are traveling-wave amplifiers, which are widely used because they amplify signals with a single pass and have a large bandwidth. SOAs have a core made of InGaAsP for gain and InP cladding layers. External pumping by current injection provides carriers that undergo stimulated emission to amplify optical signals. Amplifier gain increases with length and current but saturates with increasing optical power due to depletion of excited carriers.
A Bioamplifier is an electrophysiological device, a variation of the instrumentation amplifier, used to gather and increase the signal integrity of physiologic electrical activity for output to various sources. It may be an independent unit, or integrated into the electrodes.
The document discusses lasers, including:
- LASER is an acronym for Light Amplification by Stimulated Emission of Radiation.
- Lasers were invented in 1958 and are based on Einstein's idea of particle-wave duality of light.
- The key principles of lasers are stimulated emission within an amplifying medium and population inversion within an optical resonator.
- Common laser types discussed include ruby, He-Ne, argon ion, CO2, excimer, and solid-state lasers like Nd:YAG.
This document discusses different types of filters. It describes high-pass filters, which pass high frequencies and block low frequencies. It also describes low-pass filters, which do the opposite by passing low frequencies and blocking high frequencies. The document provides examples of passive and active low-pass filter circuits and discusses their applications in areas like telephone lines, acoustics, and radio transmitters. The objective is to study the characteristics of passive low-pass filters and measure their cut-off frequency.
The document discusses biopotential electrodes and microelectrodes. Biopotential electrodes measure bioelectric potentials through the metal-electrolyte interface between the electrode and body tissues. Microelectrodes are very small electrodes that can penetrate individual cells to obtain intracellular readings. There are two main types of microelectrodes: metal microelectrodes made of thin wires coated with insulation, and micropipette electrodes made of glass pipettes filled with electrolytes compatible with cell fluids. Both types allow precise measurement of electric potentials within cells.
Graphic record heart sound - Phonogram.
Recording the sounds connected with the pumping action of heart.
Sound from heart – phonocardiogram
Instrument to measure this – phonocardiograph
Basic function – to pick up the different heart sound,filter the required and display.
This document discusses laser diodes and their operation. It describes how population inversion is achieved through optical pumping, allowing for stimulated emission to produce coherent light amplification. A laser diode uses a p-n junction with heavily doped active regions to generate population inversion. Optical feedback is provided by a Fabry-Perot cavity formed between two mirrors to reflect photons through the gain region multiple times, producing lasing. Laser diodes can operate at nanosecond speeds and produce narrow spectral output useful for applications like optical communication and barcoding.
Laser diode have to have a specific architecture in order to optimize the laser light leaving the waveguide. There are various factors that are to be precisely noted and put into certain equations in order to calculate the differential quantum efficiency and to improvise the design of the diode lasers. The slides explain about reservoir analogy, threshold and gain and photon density as well as carrier density rate equations. Glad if it helps :)
Isolation amplifiers provide electrical isolation and safety barriers between input and output stages. They use transformer, optical, or capacitive isolation methods and isolated power supplies to break continuity while amplifying low-level signals. Common applications include medical equipment, industrial processes, and data acquisition where electrical isolation is needed to protect patients or eliminate noise.
Lasers in medicine, basic principles and applicationAugustine raj
This document discusses the principles of lasers, including:
1) Lasers work using the principle of stimulated emission of radiation, where atoms or molecules in an excited state emit photons when stimulated by an external source, producing an intense beam of coherent and monochromatic light.
2) Key terms include absorption, emission, population inversion, and stimulated emission, which are required for lasers to function.
3) Lasers have characteristics like monochromaticity, coherence, and collimation that make them useful surgical tools, though they also have disadvantages like cost and safety hazards that require special training.
This document discusses photodetectors and their applications. Photodetectors convert optical signals to electrical signals and are the fundamental component of optical receivers. They work on the principle of the photoelectric effect. Good photodetectors have high sensitivity at desired wavelengths, fast response time, compatibility with system dimensions, low noise, insensitivity to temperature, and long operating life at a reasonable cost. Applications of photodetectors include fiber optic communications, safety and security systems, process control, environmental sensing, astronomy, and defense. The document outlines specific examples and uses of photodetectors in each of these application areas.
This document discusses optical amplifiers and their future uses. It introduces different types of optical amplifiers including erbium doped fiber amplifiers and semiconductor optical amplifiers. Erbium doped fiber amplifiers were first demonstrated in the 1980s and have wide bandwidth, high gain, and are fiber compatible. Semiconductor optical amplifiers can provide exponential gain increases with length and operate from 1250-1650 nm. The document also discusses amplifier comparisons, gain dynamics, noise in optical amplifiers, and applications of optical amplifiers including 5G networks and long haul communications.
The document discusses how exercise affects the cardiovascular system. It explains that exercise causes an increase in heart rate, cardiac output, and blood pressure as the heart works to meet the increased demand of active muscles. Key measurements taken before and after exercise include systolic, diastolic, and mean arterial blood pressures as well as heart rate. Comparing these values allows inferences about how cardiac output and peripheral vascular resistance change with exercise.
Photodetectors convert optical signals to electrical signals and are the fundamental component of optical receivers. The most common photodetectors are photodiodes, which come in PIN and avalanche photodiode (APD) varieties. PIN photodiodes simply convert light to current, while APDs provide internal gain through impact ionization but introduce excess noise. Key requirements for photodetectors include sensitivity at desired wavelengths, fast response time, low noise, and insensitivity to temperature.
This document discusses biomedical sensors that use optical fibers. It introduces fiber optic sensors and their advantages, such as flexibility, lightness, safety, and immunity to electromagnetic interference. It then describes several specific fiber optic sensors: a pressure sensor that uses a Fabry-Perot cavity, temperature sensors that use phase interference or fiber deformation, and a blood flow sensor that uses laser Doppler flowmetry. Commercially available products are provided as examples for the pressure, temperature, and blood flow sensors. The document concludes that fiber optic sensors are well-suited for a variety of medical measurements due to their low cost, ease of use, and performance comparable to electric sensors.
This document provides an overview of optical fiber communication (OFC). It begins with the historical development and need for optical systems due to limitations of traditional communication methods. The basics of OFC are explained, including the system block diagram and principles of operation using ray theory and total internal reflection. Fiber types and transmission characteristics such as attenuation and bandwidth are covered. Finally, key optical components used in OFC systems such as fiber splices, connectors, and couplers are outlined.
This document discusses solid state lasers. It begins by explaining what a laser is and how it produces light through stimulated emission. It then describes the common components of all lasers including the active medium, excitation mechanism, and high reflectance mirrors. Solid state lasers use a crystalline or glass host material doped with ions like neodymium or ytterbium as the active medium. Examples given are ruby and Nd:YAG lasers. Solid state lasers have advantages like simple construction and lower cost compared to gas lasers, though their output power is not as high. Applications include drilling metals, endoscopy, and military targeting systems.
This document outlines the course structure and content for an introduction to laser theory class. The course will include 12 lectures, 4 homework assignments, a midterm exam, final exam, and individual reports. Key topics that will be covered include laser fundamentals, energy levels, rate equations, cavity design, gas lasers, solid state lasers, semiconductor lasers, and other laser types. Lasers can be classified based on their operation mode, population inversion mechanism, or active medium used. The goal is for students to understand the basic scientific principles that enable laser operation.
LEDs are of interest for fibre optics because of five inherent characteristics..
How it works?
Spectrum of an LED
Modulation of LED
LED Vs. Laser diode
disadvantages of LED
The document discusses photodetectors and the principles of p-n junction photodiodes. It describes the depletion region of a reverse biased p-n junction and how electron-hole pairs generated by photons are separated by the electric field. It also discusses pin photodiodes and how their intrinsic region allows for higher quantum efficiency and modulation frequencies compared to p-n junction photodiodes. Absorption coefficients of various semiconductor materials are shown as well as how direct and indirect bandgap materials differ in photon absorption.
The Mach-Zehnder interferometer splits a light beam into two paths using a beam splitter, then recombines the paths using a second beam splitter. It works by creating constructive and destructive interference between the light from the two paths. When the path length difference δ is 0, there is constructive interference at detector A and destructive interference at detector B. By varying δ, the probability of detection can be varied between the two detectors, making the Mach-Zehnder interferometer useful for precision measurements of small displacements, refractive index changes, and surface irregularities.
This document outlines the objectives and outcomes of the course EC8751-Optical Communication. The key objectives are to study optical fiber modes, materials, fabrication, transmission characteristics, optical sources and detectors, receiver systems, and measurements. The outcomes are to understand basic fiber elements, analyze dispersion and polarization techniques, design optical components, construct receiver systems, and design communication systems and networks. It provides textbook references and outlines topics like fiber structure, types, applications, generation of optical fiber communication systems, and fiber materials.
Optical fibers transmit light and operate based on the principles of total internal reflection. They consist of a core and cladding material, with the core having a higher refractive index. This allows light to be guided along the fiber due to total internal reflection at the core-cladding boundary. There are two main types of optical fibers - single-mode fibers which only allow one mode of light to propagate, and multi-mode fibers which allow multiple light modes. Dispersion and attenuation are two factors that limit the performance of optical fibers by causing light pulses to broaden as they travel along the fiber.
Optical fibers carry light along their length and are used for fiber-optic communications. They allow transmission over longer distances and higher data rates than other forms of communication. Fibers have a glass or plastic core that carries light through total internal reflection. They are used for long-distance communication networks, local area networks, and other applications due to advantages over metal wires like lower loss and immunity to electromagnetic interference.
Spectroscopy is the study of the interaction between electromagnetic radiation and matter. A spectrometer is used to measure the presence of compounds in a molecule by analyzing the spectrum produced when matter interacts with different wavelengths of light. Absorption spectroscopy involves matter absorbing radiation and undergoing an electronic transition to a higher energy state. UV/visible spectroscopy uses this technique to study electronic transitions in atoms and molecules in the ultraviolet and visible light ranges.
This document discusses laser diodes and their operation. It describes how population inversion is achieved through optical pumping, allowing for stimulated emission to produce coherent light amplification. A laser diode uses a p-n junction with heavily doped active regions to generate population inversion. Optical feedback is provided by a Fabry-Perot cavity formed between two mirrors to reflect photons through the gain region multiple times, producing lasing. Laser diodes can operate at nanosecond speeds and produce narrow spectral output useful for applications like optical communication and barcoding.
Laser diode have to have a specific architecture in order to optimize the laser light leaving the waveguide. There are various factors that are to be precisely noted and put into certain equations in order to calculate the differential quantum efficiency and to improvise the design of the diode lasers. The slides explain about reservoir analogy, threshold and gain and photon density as well as carrier density rate equations. Glad if it helps :)
Isolation amplifiers provide electrical isolation and safety barriers between input and output stages. They use transformer, optical, or capacitive isolation methods and isolated power supplies to break continuity while amplifying low-level signals. Common applications include medical equipment, industrial processes, and data acquisition where electrical isolation is needed to protect patients or eliminate noise.
Lasers in medicine, basic principles and applicationAugustine raj
This document discusses the principles of lasers, including:
1) Lasers work using the principle of stimulated emission of radiation, where atoms or molecules in an excited state emit photons when stimulated by an external source, producing an intense beam of coherent and monochromatic light.
2) Key terms include absorption, emission, population inversion, and stimulated emission, which are required for lasers to function.
3) Lasers have characteristics like monochromaticity, coherence, and collimation that make them useful surgical tools, though they also have disadvantages like cost and safety hazards that require special training.
This document discusses photodetectors and their applications. Photodetectors convert optical signals to electrical signals and are the fundamental component of optical receivers. They work on the principle of the photoelectric effect. Good photodetectors have high sensitivity at desired wavelengths, fast response time, compatibility with system dimensions, low noise, insensitivity to temperature, and long operating life at a reasonable cost. Applications of photodetectors include fiber optic communications, safety and security systems, process control, environmental sensing, astronomy, and defense. The document outlines specific examples and uses of photodetectors in each of these application areas.
This document discusses optical amplifiers and their future uses. It introduces different types of optical amplifiers including erbium doped fiber amplifiers and semiconductor optical amplifiers. Erbium doped fiber amplifiers were first demonstrated in the 1980s and have wide bandwidth, high gain, and are fiber compatible. Semiconductor optical amplifiers can provide exponential gain increases with length and operate from 1250-1650 nm. The document also discusses amplifier comparisons, gain dynamics, noise in optical amplifiers, and applications of optical amplifiers including 5G networks and long haul communications.
The document discusses how exercise affects the cardiovascular system. It explains that exercise causes an increase in heart rate, cardiac output, and blood pressure as the heart works to meet the increased demand of active muscles. Key measurements taken before and after exercise include systolic, diastolic, and mean arterial blood pressures as well as heart rate. Comparing these values allows inferences about how cardiac output and peripheral vascular resistance change with exercise.
Photodetectors convert optical signals to electrical signals and are the fundamental component of optical receivers. The most common photodetectors are photodiodes, which come in PIN and avalanche photodiode (APD) varieties. PIN photodiodes simply convert light to current, while APDs provide internal gain through impact ionization but introduce excess noise. Key requirements for photodetectors include sensitivity at desired wavelengths, fast response time, low noise, and insensitivity to temperature.
This document discusses biomedical sensors that use optical fibers. It introduces fiber optic sensors and their advantages, such as flexibility, lightness, safety, and immunity to electromagnetic interference. It then describes several specific fiber optic sensors: a pressure sensor that uses a Fabry-Perot cavity, temperature sensors that use phase interference or fiber deformation, and a blood flow sensor that uses laser Doppler flowmetry. Commercially available products are provided as examples for the pressure, temperature, and blood flow sensors. The document concludes that fiber optic sensors are well-suited for a variety of medical measurements due to their low cost, ease of use, and performance comparable to electric sensors.
This document provides an overview of optical fiber communication (OFC). It begins with the historical development and need for optical systems due to limitations of traditional communication methods. The basics of OFC are explained, including the system block diagram and principles of operation using ray theory and total internal reflection. Fiber types and transmission characteristics such as attenuation and bandwidth are covered. Finally, key optical components used in OFC systems such as fiber splices, connectors, and couplers are outlined.
This document discusses solid state lasers. It begins by explaining what a laser is and how it produces light through stimulated emission. It then describes the common components of all lasers including the active medium, excitation mechanism, and high reflectance mirrors. Solid state lasers use a crystalline or glass host material doped with ions like neodymium or ytterbium as the active medium. Examples given are ruby and Nd:YAG lasers. Solid state lasers have advantages like simple construction and lower cost compared to gas lasers, though their output power is not as high. Applications include drilling metals, endoscopy, and military targeting systems.
This document outlines the course structure and content for an introduction to laser theory class. The course will include 12 lectures, 4 homework assignments, a midterm exam, final exam, and individual reports. Key topics that will be covered include laser fundamentals, energy levels, rate equations, cavity design, gas lasers, solid state lasers, semiconductor lasers, and other laser types. Lasers can be classified based on their operation mode, population inversion mechanism, or active medium used. The goal is for students to understand the basic scientific principles that enable laser operation.
LEDs are of interest for fibre optics because of five inherent characteristics..
How it works?
Spectrum of an LED
Modulation of LED
LED Vs. Laser diode
disadvantages of LED
The document discusses photodetectors and the principles of p-n junction photodiodes. It describes the depletion region of a reverse biased p-n junction and how electron-hole pairs generated by photons are separated by the electric field. It also discusses pin photodiodes and how their intrinsic region allows for higher quantum efficiency and modulation frequencies compared to p-n junction photodiodes. Absorption coefficients of various semiconductor materials are shown as well as how direct and indirect bandgap materials differ in photon absorption.
The Mach-Zehnder interferometer splits a light beam into two paths using a beam splitter, then recombines the paths using a second beam splitter. It works by creating constructive and destructive interference between the light from the two paths. When the path length difference δ is 0, there is constructive interference at detector A and destructive interference at detector B. By varying δ, the probability of detection can be varied between the two detectors, making the Mach-Zehnder interferometer useful for precision measurements of small displacements, refractive index changes, and surface irregularities.
This document outlines the objectives and outcomes of the course EC8751-Optical Communication. The key objectives are to study optical fiber modes, materials, fabrication, transmission characteristics, optical sources and detectors, receiver systems, and measurements. The outcomes are to understand basic fiber elements, analyze dispersion and polarization techniques, design optical components, construct receiver systems, and design communication systems and networks. It provides textbook references and outlines topics like fiber structure, types, applications, generation of optical fiber communication systems, and fiber materials.
Optical fibers transmit light and operate based on the principles of total internal reflection. They consist of a core and cladding material, with the core having a higher refractive index. This allows light to be guided along the fiber due to total internal reflection at the core-cladding boundary. There are two main types of optical fibers - single-mode fibers which only allow one mode of light to propagate, and multi-mode fibers which allow multiple light modes. Dispersion and attenuation are two factors that limit the performance of optical fibers by causing light pulses to broaden as they travel along the fiber.
Optical fibers carry light along their length and are used for fiber-optic communications. They allow transmission over longer distances and higher data rates than other forms of communication. Fibers have a glass or plastic core that carries light through total internal reflection. They are used for long-distance communication networks, local area networks, and other applications due to advantages over metal wires like lower loss and immunity to electromagnetic interference.
Spectroscopy is the study of the interaction between electromagnetic radiation and matter. A spectrometer is used to measure the presence of compounds in a molecule by analyzing the spectrum produced when matter interacts with different wavelengths of light. Absorption spectroscopy involves matter absorbing radiation and undergoing an electronic transition to a higher energy state. UV/visible spectroscopy uses this technique to study electronic transitions in atoms and molecules in the ultraviolet and visible light ranges.
Optical spectroscopy instruments are constructed from components including a radiant energy source, wavelength selector, sample containers, radiation detector, and signal processor. Spectrometers employ various sources such as tungsten filament lamps, hydrogen/deuterium lamps, and lasers. Wavelength selection is performed using filters or monochromators containing prisms or diffraction gratings. Radiation is detected using phototubes, photomultiplier tubes, or silicon photodiodes. Instruments can be single beam, double beam in space, or double beam in time. Molecular absorption spectroscopy utilizes these components to study electronic and vibrational transitions using UV-Vis light.
Molecular fluorescence spectroscopy involves exciting molecules with UV light, causing them to emit light. The emitted light is analyzed to determine the structure of the molecule's vibrational energy levels. Fluorescence spectroscopy instruments use a xenon lamp light source, monochromators to select excitation and emission wavelengths, and a photomultiplier tube detector to measure the emitted fluorescent light. Analysis of the emitted light frequencies and intensities can provide information about the molecule's structure.
Instrumentation of uv visible spectrophotometerTalha Liaqat
A spectrophotometer is an apparatus for measuring the intensity of light in a part of the spectrum, especially as transmitted or emitted by particular substances. The instrumentation of the Spectrophotometer is described in this presentation.
A spectrophotometer uses light to measure the concentration of solutes in solution. It works by passing light through a sample in a cuvette and measuring the amount of light absorbed. The main components are a light source, monochromator to separate wavelengths, sample cuvette, detector, and display. Common light sources are tungsten halogen lamps and xenon flash lamps. Monochromators use dispersion devices like prisms, filters, or diffraction gratings. Detectors convert light to electrical signals and displays show output. Measurements rely on Beer's Law relating absorption to concentration.
Uv spectroscopy instrumentation, by dr. umesh kumar sharma & amp; shyma m sDr. UMESH KUMAR SHARMA
This document describes the instrumentation of UV-Visible spectroscopy. It discusses the key components of UV-Visible spectrophotometers including radiation sources such as tungsten lamps and deuterium lamps, wavelength selectors like monochromators and filters, sample containers, and detectors. It provides detailed diagrams of the internal components and systematic design of UV-Visible spectrophotometers. Various parts of the instrument like the radiation source, sample cell, and detector are explained.
This document provides an overview of UV-Visible spectroscopy. It discusses the basic principles, components, and types of UV-Visible spectrophotometers. The key components include a light source, monochromator, cuvettes to hold samples, and detectors. It also describes the principles of absorption spectroscopy and how double-beam spectrophotometers work by splitting the light source into reference and sample beams to improve accuracy. UV-Visible spectroscopy is a common technique for quantitative analysis that measures how light is absorbed by molecules at different wavelengths.
Spectrophotometry uses spectrophotometers to measure how much light is absorbed by a sample as a function of wavelength. A spectrophotometer directs light from a source through a sample and measures the amount of light transmitted. There are two main types - single beam spectrometers which measure one sample at a time, and double beam spectrometers which simultaneously measure a sample and reference. Spectrophotometers can be classified by the wavelength range used such as visible, UV, or infrared. They consist of a light source, dispersion elements, focusing elements, sample cells, detectors, and displays. Spectrophotometers are used to determine concentrations, identify compounds, and measure color.
This document discusses the principles, instrumentation, and applications of UV spectroscopy. It begins with an introduction to UV spectroscopy and its uses in qualitative and quantitative analysis. It then covers the underlying principles of UV absorption, including Lambert's law and Beer's law. The key components of a UV spectrophotometer are described, including radiation sources, monochromators, sample containers, detectors, and recording systems. Finally, common applications of UV spectroscopy are outlined, such as determining functional groups, conjugation, and reaction monitoring.
UV SPECTROSCOPY AND INSTRUMENTATION .INSTRUMENTAL METHODS OF ANALYSIS, B.PHARM 7TH SEM. AND FOR BSC,MSC CHEMISTRY.
This is Geeta prasad kashyap (Asst. Professor), SVITS, Bilaspur (C.G) 495001
Spectroscopy is the study of the absorption and emission of light by matter. Spectrometry is the measurement of interactions between light and matter through analysis of radiation intensity and wavelength. Ultraviolet spectroscopy works by absorbing UV light, which produces a distinct spectrum that aids in compound identification. The basic components of a UV spectrometer are a light source, monochromator, sample and reference cells, detector, amplifier, and recording device to measure and analyze absorption spectra. UV spectroscopy can be used to detect impurities through observation of additional absorption peaks.
UV-visible spectrophotometers have five main components: a light source, filters or monochromator, sample compartment, detector, and recorder. Common light sources include tungsten lamps for the visible region and deuterium lamps for the UV region. Filters and monochromators are used to select the wavelength of light. Samples are placed in the sample compartment for analysis. Detectors such as photodiodes, photomultiplier tubes, or barrier layer cells convert light signals to electrical signals. The signals are then recorded to obtain a spectrum.
This document provides an overview of analytical instruments and their working principles. It discusses the key elements of analytical instruments including radiation sources, electromagnetic radiation, interaction of radiation with matter, and various detectors. The working is based on absorption of electromagnetic radiations by samples. It also describes common analytical techniques like absorption spectrometry and discusses Beer-Lambert law and its applications. Examples of specific instruments discussed include UV-visible spectrophotometer, IR spectrophotometer, and their components and functioning.
Lasers in urology provides an overview of lasers and their applications in urology. It discusses the basic principles of lasers, including how they work by stimulating emission of radiation through excitation of atoms or molecules. The key components of a laser system are an optical resonating cavity containing a lasing medium, and mirrors on each end. Lasers are being increasingly used in urology for treatments like breaking up kidney stones and treating benign prostatic hyperplasia. Different types of lasers interact with tissue in various ways depending on factors like wavelength and tissue properties.
Instrumentation of uv visible spectroscopyZainab&Sons
UV-visible spectroscopy uses light in the UV and visible ranges. It works by passing light through a sample and measuring how much light is absorbed. Key components are a light source, monochromator, sample cell, detector, and recorder. For UV light a hydrogen lamp is used as the source and quartz is used for the cell and prism. It can be used to identify functional groups and conjugation, detect impurities, and determine molecular structure and in quantitative analysis. Applications include qualitative and quantitative analysis of organic compounds.
This document describes the components and operation of a spectrophotometer. The major components are a light source, monochromator, sample chamber, detector, and recorder/meter. The monochromator selects a specific wavelength of light from the polychromatic light source. Common types of monochromators include filters, prisms, and diffraction gratings. Samples are placed in cuvettes in the sample chamber. Double-beam spectrophotometers compare the light transmitted through a sample cuvette to a reference cuvette containing only solvent. Absorbance is then measured to analyze samples.
UV-visible spectroscopy uses light in the UV and visible regions to analyze molecular structure. The main components of a UV-visible spectrometer are a light source, wavelength selector like a monochromator, sample cell, detector, and recorder. Common light sources include hydrogen, deuterium, and tungsten lamps. Filters or monochromators are used to select wavelengths, and samples are placed in cuvettes. Detectors measure light intensity and are connected to a recorder. Double beam spectrometers have advantages over single beam in compensating for instrument fluctuations.
Ultraviolet-visible spectroscopy or ultraviolet-visible spectrophotometry (UV-Vis or UV/Vis) refers to absorption spectroscopy or reflectance spectroscopy in the ultraviolet-visible spectral region. Ultraviolet-Visible (UV-VIS) Spectroscopy is an analytical method that can measure the analyte quantity depending on the amount of light received by the analyte.
1. Atomic absorption spectroscopy involves exciting the electrons in metal atoms to higher energy levels using light from a hollow cathode lamp emitting a characteristic wavelength for the metal of interest.
2. When the excited metal atoms return to a lower energy state, they emit electromagnetic radiation of a specific wavelength that is measured by a detector.
3. The amount of light absorbed is directly proportional to the concentration of metal atoms, allowing the technique to be used for both qualitative and quantitative analysis of metals in solutions.
In thermogravimetric analysis, the change in weight in
relation to a change in temperature in a controlled environment is measured. Heat is used in TGA to force
reactions and physical changes in materials. Thermogravimetric analysis (TGA) is a reliable method to determine
endotherms, exotherms, measure oxidation processes, thermal stability, decomposition points of explosives,
characteristics of polymers, solvent residues, the level of organic and inorganic components of a mixture,
degradation temperatures of a material, and the absorbed moisture content of materials. Materials analyzed by
thermogravimetric analysis include explosives, petroleum, chemicals, biological samples, polymers, composites,
plastics, adhesives, coatings, organic materials, and pharmaceuticals.The thermogravimetric analysis instrument usually consists of a high-precision balance and sample pan.
The pan holds the sample
material and is located in a
furnace or oven that is
heated or cooled during the
experiment. A thermocouple
is used to accurately control
and measure the
temperature within the oven.
The mass of the sample is
constantly monitored during
the analysis. An inert or
reactive gas may be used to
purge and control the
environment. The analysis is
performed by gradually
raising the temperature and plotting the
substances weight against temperature. A
computer is utilized to control the
instrument and to process the output
curves.
Spectroscopy is the measurement and interpretation of electromagnetic radiation absorbed or emitted when the molecules or atoms or ions of a sample move from one energy state to another energy state. UV spectroscopy is a type of absorption spectroscopy in which light of the ultra-violet region (200-400 nm) is absorbed by the molecule which results in the excitation of the electrons from the ground state to a higher energy state.Basically, spectroscopy is related to the interaction of light with matter.
As light is absorbed by matter, the result is an increase in the energy content of the atoms or molecules.
When ultraviolet radiations are absorbed, this results in the excitation of the electrons from the ground state towards a higher energy state.
Molecules containing π-electrons or nonbonding electrons (n-electrons) can absorb energy in the form of ultraviolet light to excite these electrons to higher anti-bonding molecular orbitals.
The more easily excited the electrons, the longer the wavelength of light they can absorb. There are four possible types of transitions (π–π*, n–π*, σ–σ*, and n–σ*), and they can be ordered as follows: σ–σ* > n–σ* > π–π* > n–π* The absorption of ultraviolet light by a chemical compound will produce a distinct spectrum that aids in the identification of the compound.
Medical devices are heavily regulated because of their
intended uses in human beings. Generally medical devices
are classified into different categories depending upon the
degree of potential risks and regulated accordingly.Many medical devices are involved with relative moving parts,
either in contact to the native tissues or within the biomaterials,
and often under loading. Important issues, such as friction and
wear of the moving parts, not only affect the functions of these
devices but also the potential adverse effects on the natural tissues.
Biotribology deals with the application of tribological principles,
such as friction, wear and lubrication between relatively motions
surfaces, to medical and biological systems. Biotribology plays an important role in a number of medical devices
Protein based nanostructures for biomedical applications karoline Enoch
Proteins are kind of natural molecules that show unique
functionalities and properties in biological materials and
manufacturing feld. Tere are numerous nanomaterials
which are derived from protein, albumin, and gelatin. Tese
nanoparticles have promising properties like biodegradability, nonantigenicity, metabolizable, surface modifer, greater
stability during in vivo during storage, and being relatively
easy to prepare and monitor the size of the particles.
These particles have the ability to attach covalently with
drug and ligand
A Schering Bridge is a bridge circuit used for measuring an unknown electrical capacitance and its dissipation factor. The dissipation factor of a capacitor is the the ratio of its resistance to its capacitive reactance. The Schering Bridge is basically a four-arm alternating-current (AC) bridge circuit whose measurement depends on balancing the loads on its arms .
A Maxwell bridge is a modification to a Wheatstone bridge used to measure an unknown inductance (usually of low Q value) in terms of calibrated resistance and inductance or resistance and capacitance. When the calibrated components are a parallel resistor and capacitor, the bridge is known as a Maxwell-Wien bridge. It is named for James C. Maxwell, who first described it in 1873.
It uses the principle that the positive phase angle of an inductive impedance can be compensated by the negative phase angle of a capacitive impedance when put in the opposite arm and the circuit is at resonance; i.e., no potential difference across the detector (an AC voltmeter or ammeter)) and hence no current flowing through it. The unknown inductance then becomes known in terms of this capacitance.
The Kelvin bridge is a modification of the Wheatstone bridge that allows for more accurate measurement of low resistances below 1 ohm. It addresses errors that occur in Wheatstone bridge measurements of low resistances due to the resistance of connecting leads. The Kelvin bridge divides the lead resistance in half by connecting the galvanometer between the leads rather than at the resistance being measured. The Kelvin double bridge further improves accuracy by using two sets of ratio arms to effectively cancel out any remaining lead resistance effects.
Dc bridge types ,derivation and its applicationkaroline Enoch
The DC Bridge is used for measuring the unknown electrical resistance. This can be done by balancing the two legs of the bridge circuit. The value of one of the arm is known while the other of them is unknown
The bridge uses for measuring the value of unknown resistance, inductance and capacitance, is known as the AC Bridge. The AC bridges are very convenient and give the accurate result of the measurement.The construction of the bridges is very simple. The bridge has four arms, one AC supply source and the balance detector. It works on the principle that the balance ratio of the impedances will give the balance condition to the circuit which is determined by the null detector.
Photodynamic therapy (PDT) is a two-stage treatment that combines light energy with a drug (photosensitizer) designed to destroy cancerous and precancerous cells after light activation. Photosensitizers are activated by a specific wavelength of light energy, usually from a laser.
Preamplifier and impedance matching circuitskaroline Enoch
A preamplifier circuit with a very low noise characteristic can be built by simply combining a FET transistor with a bipolar one. The input impedance of the preamp circuit is almost the same as the gate impedance of the FET transistor (around 1MΩ) The output impedance at the other end is about 1KΩ.
Phototherapy is a type of medical treatment that involves exposure to fluorescent light bulbs or other sources of light like halogen lights, sunlight, and light emitting diodes (LEDs) to treat certain medical conditions
Lasers are widely used in ophthalmology to treat various eye conditions. The main types of laser-tissue interactions are photothermal, photochemical, and photoionizing effects. Different laser wavelengths are absorbed selectively by tissues like melanin, haemoglobin, and macular xanthophyll, allowing targeted treatment. Common lasers used in retina include argon, frequency-doubled Nd:YAG, krypton red, and diode lasers. Therapeutic applications include treating retinal tears, diabetic retinopathy, and choroidal neovascular membranes. Precise laser delivery systems like slit lamps and indirect ophthalmoscopes allow treatment of anterior and posterior segment diseases.
he ability of the laser to ablate prostatic tissue with minimal hemorrhage has concentrated most of the interest in urologically applied lasers to benign prostatic hyperplasia (BPH) [Anson et al. 1994]. Despite tremendous advances in the surgical and minimally invasive treatment of BPH, transurethral resection of the prostate (TURP) is still considered the ‘gold standard’. The risks of TURP are always mentioned when discussing the reasons for seeking alternative treatment modalities for BPH. Bleeding certainly remains a concern, especially in patients on some form of anticoagulation (heparin, coumarin related compounds, antiplatelet agents) or those with prostates in excess of 60–80 g. On the other hand, with the availability of transurethral resection in saline (TURiS), the TURP syndrome is nowadays considered by many to be a relatively rare complication
Lasers have been used successfully to treat a variety of vascular lesions including superficial vascular malformations (port-wine stains), facial telangiectases, haemangiomas, pyogenic granulomas, Kaposi sarcoma and poikiloderma of Civatte. Lasers that have been used to treat these conditions include argon, APTD, KTP, krypton, copper vapour, copper bromide, pulsed dye lasers and Nd:YAG. Argon (CW) causes a high degree of non-specific thermal injury and scarring and is now largely replaced by yellow-light quasi-CW and pulsed laser therapies.
The pulsed dye laser is considered the laser of choice for most vascular lesions because of its superior clinical efficacy and low-risk profile. It has a large spot size (5 to 10mm) allowing large lesions to be treated quickly. Side effects include postoperative bruising (purpura) that may last 1-2 weeks and transient pigmentary changes. Crusting, textural changes and scarring are rarely seen.
The term LASER is an acronym for ‘Light Amplification by the Stimulated Emission of Radiation’. As its first application in dentistry by Miaman, in 1960, the laser has seen various hard and soft tissue applications. In the last two decades, there has been an explosion of research studies in laser application. In hard tissue application, the laser is used for caries prevention, bleaching, restorative removal and curing, cavity preparation, dentinal hypersensitivity, growth modulation and for diagnostic purposes, whereas soft tissue application includes wound healing, removal of hyperplastic tissue to uncovering of impacted or partially erupted tooth, photodynamic therapy for malignancies, photostimulation of herpetic lesion. Use of the laser proved to be an effective tool to increase efficiency, specificity, ease, and cost and comfort of the dental treatment.
Photolithography, also called optical lithography or UV lithography, is a process used in microfabrication to pattern parts on a thin film or the bulk of a substrate (also called a wafer). It uses light to transfer a geometric pattern from a photomask (also called an optical mask) to a photosensitive (that is, light-sensitive) chemical photoresist on the substrate. A series of chemical treatments then either etches the exposure pattern into the material or enables deposition of a new material in the desired pattern upon the material underneath the photoresist. In complex integrated circuits, a CMOS wafer may go through the photolithographic cycle as many as 50 times.
Photolithography shares some fundamental principles with photography in that the pattern in the photoresist etching is created by exposing it to light, either directly (without using a mask) or with a projected image using a photomask. This procedure is comparable to a high precision version of the method used to make printed circuit boards. Subsequent stages in the process have more in common with etching than with lithographic printing. This method can create extremely small patterns, down to a few tens of nanometers in size. It provides precise control of the shape and size of the objects it creates and can create patterns over an entire surface cost-effectively. Its main disadvantages are that it requires a flat substrate to start with, it is not very effective at creating shapes that are not flat, and it can require extremely clean operating conditions. Photolithography is the standard method of printed circuit board (PCB) and microprocessor fabrication. Directed self-assembly is being evaluated as an alternative to photolithography
The document discusses piezoelectric transducers. It explains that piezoelectric transducers use piezoelectric materials that generate a voltage when pressure or stress is applied. Piezoelectric transducers convert mechanical energy into electrical energy and are used to measure physical quantities like force, pressure, and stress. Common piezoelectric materials include quartz crystals and ceramic materials that are polarized to gain piezoelectric properties. When stress is applied, the ions in the material move and generate an electrical charge that can be measured.
Photoelectric transducers and its classificationkaroline Enoch
The photoelectric transducer converts the light energy into electrical energy. It is made of semiconductor material. The photoelectric transducer uses a photosensitive element, which ejects the electrons when the beam of light absorbs through it.
Piezoresistive pressure sensors are one of the very-first products of MEMS technology. Those products are widely used in biomedical applications, automotive industry and household appliances.
The sensing material in a piezoresistive pressure sensor is a diaphragm formed on a silicon substrate, which bends with applied pressure. A deformation occurs in the crystal lattice of the diaphragm because of that bending. This deformation causes a change in the band structure of the piezoresistors that are placed on the diaphragm, leading to a change in the resistivity of the material. This change can be an increase or a decrease according to the orientation of the resistors.
Properties of Fluids, Fluid Statics, Pressure MeasurementIndrajeet sahu
Properties of Fluids: Density, viscosity, surface tension, compressibility, and specific gravity define fluid behavior.
Fluid Statics: Studies pressure, hydrostatic pressure, buoyancy, and fluid forces on surfaces.
Pressure at a Point: In a static fluid, the pressure at any point is the same in all directions. This is known as Pascal's principle. The pressure increases with depth due to the weight of the fluid above.
Hydrostatic Pressure: The pressure exerted by a fluid at rest due to the force of gravity. It can be calculated using the formula P=ρghP=ρgh, where PP is the pressure, ρρ is the fluid density, gg is the acceleration due to gravity, and hh is the height of the fluid column above the point in question.
Buoyancy: The upward force exerted by a fluid on a submerged or partially submerged object. This force is equal to the weight of the fluid displaced by the object, as described by Archimedes' principle. Buoyancy explains why objects float or sink in fluids.
Fluid Pressure on Surfaces: The analysis of pressure forces on surfaces submerged in fluids. This includes calculating the total force and the center of pressure, which is the point where the resultant pressure force acts.
Pressure Measurement: Manometers, barometers, pressure gauges, and differential pressure transducers measure fluid pressure.
Supermarket Management System Project Report.pdfKamal Acharya
Supermarket management is a stand-alone J2EE using Eclipse Juno program.
This project contains all the necessary required information about maintaining
the supermarket billing system.
The core idea of this project to minimize the paper work and centralize the
data. Here all the communication is taken in secure manner. That is, in this
application the information will be stored in client itself. For further security the
data base is stored in the back-end oracle and so no intruders can access it.
Prediction of Electrical Energy Efficiency Using Information on Consumer's Ac...PriyankaKilaniya
Energy efficiency has been important since the latter part of the last century. The main object of this survey is to determine the energy efficiency knowledge among consumers. Two separate districts in Bangladesh are selected to conduct the survey on households and showrooms about the energy and seller also. The survey uses the data to find some regression equations from which it is easy to predict energy efficiency knowledge. The data is analyzed and calculated based on five important criteria. The initial target was to find some factors that help predict a person's energy efficiency knowledge. From the survey, it is found that the energy efficiency awareness among the people of our country is very low. Relationships between household energy use behaviors are estimated using a unique dataset of about 40 households and 20 showrooms in Bangladesh's Chapainawabganj and Bagerhat districts. Knowledge of energy consumption and energy efficiency technology options is found to be associated with household use of energy conservation practices. Household characteristics also influence household energy use behavior. Younger household cohorts are more likely to adopt energy-efficient technologies and energy conservation practices and place primary importance on energy saving for environmental reasons. Education also influences attitudes toward energy conservation in Bangladesh. Low-education households indicate they primarily save electricity for the environment while high-education households indicate they are motivated by environmental concerns.
Tools & Techniques for Commissioning and Maintaining PV Systems W-Animations ...Transcat
Join us for this solutions-based webinar on the tools and techniques for commissioning and maintaining PV Systems. In this session, we'll review the process of building and maintaining a solar array, starting with installation and commissioning, then reviewing operations and maintenance of the system. This course will review insulation resistance testing, I-V curve testing, earth-bond continuity, ground resistance testing, performance tests, visual inspections, ground and arc fault testing procedures, and power quality analysis.
Fluke Solar Application Specialist Will White is presenting on this engaging topic:
Will has worked in the renewable energy industry since 2005, first as an installer for a small east coast solar integrator before adding sales, design, and project management to his skillset. In 2022, Will joined Fluke as a solar application specialist, where he supports their renewable energy testing equipment like IV-curve tracers, electrical meters, and thermal imaging cameras. Experienced in wind power, solar thermal, energy storage, and all scales of PV, Will has primarily focused on residential and small commercial systems. He is passionate about implementing high-quality, code-compliant installation techniques.
Applications of artificial Intelligence in Mechanical Engineering.pdfAtif Razi
Historically, mechanical engineering has relied heavily on human expertise and empirical methods to solve complex problems. With the introduction of computer-aided design (CAD) and finite element analysis (FEA), the field took its first steps towards digitization. These tools allowed engineers to simulate and analyze mechanical systems with greater accuracy and efficiency. However, the sheer volume of data generated by modern engineering systems and the increasing complexity of these systems have necessitated more advanced analytical tools, paving the way for AI.
AI offers the capability to process vast amounts of data, identify patterns, and make predictions with a level of speed and accuracy unattainable by traditional methods. This has profound implications for mechanical engineering, enabling more efficient design processes, predictive maintenance strategies, and optimized manufacturing operations. AI-driven tools can learn from historical data, adapt to new information, and continuously improve their performance, making them invaluable in tackling the multifaceted challenges of modern mechanical engineering.
Accident detection system project report.pdfKamal Acharya
The Rapid growth of technology and infrastructure has made our lives easier. The
advent of technology has also increased the traffic hazards and the road accidents take place
frequently which causes huge loss of life and property because of the poor emergency facilities.
Many lives could have been saved if emergency service could get accident information and
reach in time. Our project will provide an optimum solution to this draw back. A piezo electric
sensor can be used as a crash or rollover detector of the vehicle during and after a crash. With
signals from a piezo electric sensor, a severe accident can be recognized. According to this
project when a vehicle meets with an accident immediately piezo electric sensor will detect the
signal or if a car rolls over. Then with the help of GSM module and GPS module, the location
will be sent to the emergency contact. Then after conforming the location necessary action will
be taken. If the person meets with a small accident or if there is no serious threat to anyone’s
life, then the alert message can be terminated by the driver by a switch provided in order to
avoid wasting the valuable time of the medical rescue team.
Determination of Equivalent Circuit parameters and performance characteristic...pvpriya2
Includes the testing of induction motor to draw the circle diagram of induction motor with step wise procedure and calculation for the same. Also explains the working and application of Induction generator
ELS: 2.4.1 POWER ELECTRONICS Course objectives: This course will enable stude...Kuvempu University
Introduction - Applications of Power Electronics, Power Semiconductor Devices, Control Characteristics of Power Devices, types of Power Electronic Circuits. Power Transistors: Power BJTs: Steady state characteristics. Power MOSFETs: device operation, switching characteristics, IGBTs: device operation, output and transfer characteristics.
Thyristors - Introduction, Principle of Operation of SCR, Static Anode- Cathode Characteristics of SCR, Two transistor model of SCR, Gate Characteristics of SCR, Turn-ON Methods, Turn-OFF Mechanism, Turn-OFF Methods: Natural and Forced Commutation – Class A and Class B types, Gate Trigger Circuit: Resistance Firing Circuit, Resistance capacitance firing circuit.
Sri Guru Hargobind Ji - Bandi Chor Guru.pdfBalvir Singh
Sri Guru Hargobind Ji (19 June 1595 - 3 March 1644) is revered as the Sixth Nanak.
• On 25 May 1606 Guru Arjan nominated his son Sri Hargobind Ji as his successor. Shortly
afterwards, Guru Arjan was arrested, tortured and killed by order of the Mogul Emperor
Jahangir.
• Guru Hargobind's succession ceremony took place on 24 June 1606. He was barely
eleven years old when he became 6th Guru.
• As ordered by Guru Arjan Dev Ji, he put on two swords, one indicated his spiritual
authority (PIRI) and the other, his temporal authority (MIRI). He thus for the first time
initiated military tradition in the Sikh faith to resist religious persecution, protect
people’s freedom and independence to practice religion by choice. He transformed
Sikhs to be Saints and Soldier.
• He had a long tenure as Guru, lasting 37 years, 9 months and 3 days
2. INTRODUCTION
• The common feature of spectroscopic measurements is that they all
measure some spectroscopic properties that are related to the composition
and structure of biochemical species in the sample of interest.
• There are several types of spectroscopic measurements: absorption,
scattering (elastic and inelastic), and emission.
• A typical spectroscopic experiment that allows us to analyze complex
biological systems is conceptually simple.
• Light at a certain wavelength λ (or frequency ν = c/λ) is used to irradiate a
sample of interest. This process is called excitation.
3. CONTD..
• Properties of the light that then emerges from the sample are measured and
analyzed.
• Some properties deal with the fraction of the incident radiation absorbed by the
sample: the techniques involved are collectively called absorption spectroscopy
(e.g., ultraviolet [UV], visible, and infrared (IR) absorption techniques).
• Other properties are related to the incident radiation reflected back from the
samples (elastic scattering [ES] techniques).
• Alternatively, one can measure the light emitted or scattered by the sample,
involving processes that occur at wavelengths different from the excitation
wavelength; the techniques involved are fluorescence, phosphorescence, and
inelastic scattering (Raman scattering).
4. CONTD..
• Other specialized techniques can be used to detect specific properties of the
emitted light, such as its degree of polarization and decay times.
• The range of wavelengths used in various types of molecular spectroscopy
to study biological molecules is quite extensive.
5. COMPONENTS OF BASIC SPECTROPHOTOMETER
1. An excitation light source
2. A dispersive device (optical filters, monochromators, or polychromators)
3. A sample to be analyzed (usually in a compartment having a sample holder)
4. A photometric detector (equipped with a readout device)
6. • The recorded spectra (absorption, reflection,scattering, emission, or excitation)
represent the photon emission rate or power recorded at each wavelength over a
wavelength interval determined by the slit widths and dispersion of the
monochromator.
• There are a large variety of manufacturers of spectrometers, each offering several
models with different performance characteristics and each offering different options.
• Basic instrumentation components are also commercially available. For special
applications, an investigator may assemble off-the-shelf components for his or her
particular applications.
• The basic components can be adapted to design the instrument for each type of
spectroscopic measurements.
7. ABSORPTION MEASUREMENTS
• The collimated output of a light source is focused on the entrance slit of an
excitation monochromator for wavelength scanning.
• The output of the excitation monochromator is directed to the sample
inside the sample compartment.
• The light transmitted by the sample is collected through appropriate optics
and focused onto a detector.
8. .
• This simple instrumental setup is often used in a single-beam absorption
spectrometer.
• Double-beam instruments include a reference beam, which is used to
automatically correct intensity fluctuations in the light source in order to
reduce electronic drift and lamp warm-up periods
9. SCATTERING MEASUREMENTS
• The ES technique involves detection of the backscattering of a broadband
light source irradiating the sample of interest
• A spectrometer records the backscattered light at various wavelengths and
produces a spectrum that is dependent on sample structure, as well as
chromophore constituents.
• In general, the sample is illuminated with the excitation light, which is
selected with a dispersive element and then directed to a specific point
location (e.g., via an optical fiber) of the sample
10. CONTD..
• The scattered light is measured at the same wavelength as the excitation
wavelength.
• With inelastic scattering measurements, one measures the scattered light
from the sample in a spectral region different from the excitation
wavelength.
• In this case, the basic setup is similar to the ES setup but has an
additional dispersive element to analyze the scattered emission from the
samples
13. EMISSION MEASUREMENTS
• The excitation light source is usually a laser or high-intensity xenon
arc lamp.
• The collimated output of the light source is focused on the entrance
slit of an excitation monochromator.
• The output of the excitation monochromator is directed to the
sample. When a laser is used as the excitation source, the excitation
monochromator is not required.
14. • The emission from the sample is collected through appropriate optics
and focused onto the entrance slit of an emission monochromator.
• The excitation beam and the emission beam are usually focused at
right angles for minimum interference from scattered light.
15.
16. EXCITATION LIGHT SOURCES
• UV light is generally used for excitation in many spectroscopic
measurements.
• These UV sources may be classified into two categories, namely, line
or continuum type, and can be used in a continuous wave (CW) mode
or in a pulsed mode.
• The line sources provide sharp spectral lines, whereas continuum
sources exhibit a broadband emission.
17. HIGH-PRESSURE ARC LAMPS
• High-pressure arc lamps are the most commonly used radiation sources.
• These lamps produce an intense quasi continuum radiation ranging from
the UV (1000 nm) with only a few broadbands at approximately 450–500
nm.
• The lamps consist of two tungsten electrodes in a quartz envelope
containing gases under high pressure, for example, xenon (Xe), mercury, or
a Xe– mercury mixture.
• Lamps of this type are commercially available in a wide range of input
power from a few watts to several kilowatts.
18. CONTD..
• The high-pressure mercury arc lamp is similar to the high-pressure xenon
lamp in appearance and performance.
• The spectral output of mercury lamps is of a line type, whereas that of the
xenon lamps is of a continuum type.
• If excitation can be carried out at only one wavelength or a few fixed
wavelengths of the mercury emission lines, the mercury lamp is probably
the most effective radiation source.
• The Xe lamp, however, is more commonly used, because it provides a
smoother spectral profile that is more suitable for conducting excitation
spectra measurements.
19. CONTD..
• The Xe arc lamp is the most versatile light
source for steady-state spectrometers and
has found widespread use.
• This lamp provides a relatively continuous
light output from 250 to 700 nm.
• Xe arc lamps emit a continuum of light as
a result of the recombination of electrons
with the ionized Xe atoms.
• Complete separation of the electrons from
the atoms yields the continuous emission.
20. CONTD..
• Xe lamps are available with an ellipsoidal reflector as part of the lamp
itself.
• Parabolic reflectors in some commercially available Xe lamps collect a
large solid angle of light and provide a collimated output.
• The operation of high-pressure arc lamps requires special care and
handling, such as reduction of the excitation stray light with a good
monochromator, use of a highly regulated direct current (dc) power
supply and removal of the heat generated by the lamp output in the
IR range.
21. CONTD..
• A warm-up period is also necessary to minimize arc wandering,
because there is always some tendency for the arc to change its
location inside the lamp envelope during the first half hour of
operation.
• This arc wandering effect may cause sudden variations in the
observed intensity, especially when the image of the arc is focused
into a small slit aperture
22. CONTD..
• Extreme care should be exercised when inspecting high-pressure arc
lamps.
• These lamps may explode when dropped or bumped because they are
filled with gases at high pressures (~5 atm at ambient temperature
and 20–30 atm at operating temperature conditions).
• It is recommended that special leather gloves, safety glasses, and
protective headgear be used whenever the lamp housing is opened.
• One should not look directly at an operating Xe lamp.
23. CONTD..
• The extreme brightness will damage the retina, and the UV light can
damage the cornea.
• With some older lamps, proper ventilation or use of deozonators is
required to remove the ozone produced by the UV radiation of the
lamp.
• Recently, many available Xe lamps are considered ozone-free since
their operation does not generate ozone in the surrounding
environment.
24. LOW-PRESSURE VAPOR LAMPS
• Low- (or medium-) pressure mercury vapor lamps are often used as
line sources.
• They are simple to use, require little power, and offer intense UV
radiation concentrated in a few lines (e.g., 253.7 nm,
365.0/265.5/366.3 nm multiplet).
• The mercury vapor lamps are widely used in simple filter-type
spectrometers because of their low cost, intense emission
characteristics, and good stability.
• The lamps do not need a complex power supply system and provide
excellent reference light sources for calibration of spectrometers.
25. INCANDESCENT LAMPS
• The tungsten filament incandescent lamp is the simplest continuum
source.
• This type of incandescent lamp exhibits a smooth, continuous spectral
profile determined by the blackbody radiation characteristics given by
Planck’s equation:
• where Sλ is the spectral radiance (W cm−2 sr−1 nm−1)
• λ is the wavelength (nm)
• T is the temperature (K)
• Eλ is the spectral emissivity of the filament material (dimensionless)
26. CONTD..
• Since incandescent lamps usually have low UV output, they are seldom
used as excitation sources, especially for luminescence measurements
where samples absorb the UV.
• Their smooth spectral profile, however, makes them very suitable for
intensity calibration procedures.
• Standard incandescent lamps with calibration data provided by the
National Institute of Standards and Technology (NIST) are readily available
commercially.
• Intensity calibration data are available for the spectral range from 250 nm
to 2.5 μm.
27. SOLID-STATE LIGHT SOURCES
• Light-emitting diodes (LEDs) are solid-state light sources, which provide output
over a wide range of wavelengths.
• These devices require little power and generate little heat. One can use a few LEDs
to cover a spectral range from 400 to 700 nm.
• LEDs are practical light sources for many low-power photonic applications. LEDs
can be amplitude modulated up to hundreds of megahertz.
• Another type of solid state light source is the solid-state laser, which is described in
the next section.
28. LASERS
• Although primarily conventional light sources have been used for absorption
analyses, lasers are increasingly used in luminescence and Raman measurements.
• The advantages offered by lasers as excitation sources include
1. Monochromaticity
2. High degree of collimation
3. High intensity
4. Phase coherence
5. Short pulse duration (with pulsed lasers)
6. Polarized radiation
29. • Selection of laser excitation sources is determined by the wavelengths that
can be matched to the absorption band of the compounds to be analyzed in
order to take advantage of maximum absorption.
• If time-resolved measurements are performed, the pulse width of the laser
is an important factor to consider.
• The intensity of a laser is very high at (or even near) the laser emission
lines and, therefore, often interferes with the lower intensity of the emission
or scattering signal being measured.
• A number of devices, such as a spike filter or a single monochromator, may
be used to reject the Rayleigh scattered light. Notch filters, which consist of
crystalline arrays of polystyrene spheres, exhibit very high-efficiency
rejection of laser lines.
30. OPTICAL FILTERS
• Optical filters are passive devices that
allow the transmission of a specific
wavelength or set of wavelengths of
light.
There are two classes of optical
filters that have different
mechanisms of operation:
Absorptive filters
Dichroic filters.
31. ABSORPTIVE FILTERS
• Absorptive filters have a coating of different organic and inorganic materials
that absorb certain wavelengths of light, thus allowing the desired
wavelengths to pass through.
• Since they absorb light energy, the temperature of these filters increases
during operation.
• They are simple filters and can be added to plastics to make less costly
filters than their glass-based counterparts.
• The operation of these filters does not depend on the angle of the incident
light but on the properties of the material that makes up the filters.
• As a result, they are good filters to use when reflected light of the
unwanted wavelength can cause noise in optical signal.
32. DICHROIC FILTERS
• Dichroic filters are more complicated in their operation.
• They consist of a series of optical coatings with precise thicknesses that are
designed to reflect unwanted wavelengths and transmit the desired
wavelength range.
• This is achieved by causing the desired wavelengths to interfere
constructively on the transmission side of the filter, while other
wavelengths interfere constructively on the reflection side of the filter
34. SHORTPASS FILTER
A shortpass filter allows
shorter wavelengths than the
cut-off wavelength to pass
through, while it attenuates
longer wavelengths
35. LONGPASS FILTER
A longpass filter transmits
longer wavelengths than the
cut-on wavelength while it
blocks shorter wavelengths
36. BANDPASS FILTER
• A bandpass filter is a filter that lets a
particular range, or “band”, of
wavelengths to go through, but
attenuates all wavelengths around
the band.
• A monochromatic filter is an extreme
case of a bandpass filter, which
transmits only a very narrow range
of wavelengths
37. IMPORTANCE OF OPTICAL
FILTERS
• Optical filters are very important in laser experiment applications.
• Laser safety glasses are optical filters you can wear to protect your eyes from laser
radiation.
• They typically filter out a small range of wavelengths to allow you to see your
surroundings while working on an optical experiment while a laser is turned on.
• It is very important to wear safety glasses that are rated for the laser wavelength
being used.
38. IMPORTANCE OF OPTICAL
FILTERS
• Optical filters are also important as optical components in laser experiments. For
example, when measuring the photoluminescence (PL) of a material, all the light
being emitted from the spot that is being excited by a laser is coupled into an
optical fiber and measured in a spectrometer.
• The light of the laser is very intense, and depending on what value the wavelength
of photoluminescence is, the laser light could overcome that signal.
• Applying a filter for the laser wavelength somewhere between the film and the lens
that collects the light coming from the film reduces or eliminates the laser peak,
allowing us to see the photoluminescence peak clearly.
39. MONOCHROMATORS
• Continuous wavelength selection is performed with monochromators, which are
used to disperse polychromatic or white light into the various colors.
• The performance specifications of a monochromator are characterized by the
spectral dispersion, the efficiency, and the stray light levels.
• The spectral dispersion is usually expressed in nanometers per millimeter, where the
slit width is expressed in millimeters.
• Low stray light and high efficiency are desired qualities in selecting a
monochromator.
• There are two types of monochromators: prism and grating monochromators.
40. PRISM MONOCHROMATORS
• In prism monochromators, light dispersion is due to the change of the refractive
index of the prism material with the wavelength of the incident light. The angular
dispersion D is given by
where θ is the angular deviation n is the refractive index of the prism materials λ is
the wavelength of the light source
41. Prism monochromators usually produce less stray light than grating devices and are
free from overlap from multiple orders, but they are less convenient to use than
grating monochromators due to their nonlinear scanning dispersion.
42. GRATING MONOCHROMATORS
• Most spectrometers are now equipped with monochromators having diffraction
gratings.
• Gratings comprise a large number of lines, or grooves, ruled on a highly polished
surface.
• The density and shape of its grooves determine the characteristics of a grating.
• Energy throughput and resolution increase with increasing number of grooves per
millimeter.
• The width of a groove should be approximately equal to the wavelength of the light
to be dispersed.
43. • The shape of the groove should be such that the maximum amount of light at a
given wavelength is concentrated at only one specific angle for each order.
• The design and construction of the grating also determine the other properties of
the monochromator, for example, reflectivity (or radiance throughout) and stray
light rejection
44. The general diffraction grating formula is given by
where
θ′ is the angle of incidence
n is the number of grooves per unit length
d is the groove spacing
k is the dispersion order
Most gratings used in modern spectrometers are of the reflection type (θ = θ′). In this case, the
observation is in the direction of illumination (Littrow configuration). The grating formula then
becomes
45. OPTICAL FIBERS
• A widely used component that provides an optical link between a spectroscopic
instrument and a remotely located sample is the optical fiber.
• The rapid growth of fiber-optic sensing has paralleled the commercial availability of
low-attenuation optical fibers.
• In many applications, the optical fibers comprise a core made with an optically
transparent material (e.g., glass, quartz, or polymer) with a certain refractive index,
n1, surrounded by a cladding made with another material (e.g., quartz or plastic)
having another refractive index, n2.
46. COMPONENTS OF
OPTICAL CABLE
Core - Thin glass center of the fiber where the light travels
Cladding - Outer optical material surrounding the core
that reflects the light back into the core
Buffer coating - Plastic coating that protects the fiber from
damage and moisture
optical cables -Hundreds or thousands of these optical
fibers are arranged in bundles in optical cables.
Jacket -The bundles are protected by the cable's outer
covering
47. Optical fibers can be used to
transmit the excitation light to a
sample and transmit the signal
(reflected or scattered light) from the
sample to the detector.
49. SINGLE FIBER SYSTEM
• A single fiber is used to
transmit the excitation beam
from a light source to the
sample and transmit the
emission from the sample to
the detector.
• A dichroic filter is used to
transmit the light at the
excitation wavelength and
reflect light at the emission
wavelength.
50. BIFURCATED FIBER
Bifurcated fiber is used, one end
transmitting the excitation light to the
sample and the other end transmitting the
sample emission light to the detector
51. DUAL FIBER
• Two separate parallel fibers are used, one fiber
transmitting excitation light and the other fiber
transmitting the emission light.
• Two perpendicular fibers are used. The angle
between the fibers can be varied and optimized in
order to optimize the overlap of the excitation
and detection volumes and to minimize scattered
light.
52. TYPES OF OPTICAL FIBERS
The types of
optical fibers
depend on
the refractive
index,
materials
used, and
mode of
propagation
Step Index
Fibers: It
consists of a
core
surrounded by
the cladding,
which has a
single uniform
index of
refraction.
Graded Index
Fibers: The
refractive index of
the optical fiber
decreases as the
radial distance
from the fiber axis
increases.
53. TYPES OF OPTICAL
FIBRES
Based on the materials
Plastic Optical Fibers: The
polymethylmethacrylate is used as a core
material for the transmission of the light.
Glass Fibers: It consists of extremely fine
glass fibers.
54. The classification based on the mode of propagation of light is as follows:
Single-Mode Fibers: These fibers are used for long-distance transmission of signals.
Multimode Fibers: These fibers are used for short-distance transmission of signals.
55. TYPES OF FIBERS
The mode of propagation and refractive
index of the core is used to form four
combination types of optic fibers as follows:
Step index-single
mode fibers
Graded index-
Single mode fibers
Step index-
Multimode fibers
Graded index-
Multimode fibers
56. OPTICAL DETECTORS
• Detectors Selection of a suitable detector is one of the most critical steps in the
development of a spectrometer.
• Detectors for electromagnetic radiation can be classified into photoemissive,
semiconductor, and thermal types. Photoemissive detectors are generally used in
optical measurements.
• These devices include PM tubes, photodiodes (PDs), and imaging tubes.
• The PM tubes are the most commonly used because they are the most sensitive
detectors for the visible and near-UV regions.
57. There are two types of detectors, single-channel detectors and multichannel.
Multichannel detectors include 1D and 2D detector arrays.
Traditionally, spectroscopy has involved using a scanning monochromator and a
single-element detector (e.g., PD, PM. Detectors that permit the recording of the
entire spectrum simultaneously, thus providing the multiplex advantage, are known as
multichannel detectors.
With multichannel systems, a complete spectrum can be recorded in the same time it
takes to record one wavelength point with a scanning system
61. • A photomultiplier tube, useful for light detection of very weak signals, is a
photoemissive device in which the absorption of a photon results in the emission of
an electron.
• These detectors work by amplifying the electrons generated by a photocathode
exposed to a photon flux
62. • Photomultipliers acquire light through a glass or quartz window that covers a
photosensitive surface, called a photocathode, which then releases electrons that
are multiplied by electrodes known as metal channel dynodes.
• At the end of the dynode chain is an anode or collection electrode.
• Over a very large range, the current flowing from the anode to ground is directly
proportional to the photoelectron flux generated by the photocathode.
63. • The spectral response, quantum efficiency, sensitivity, and dark current of a
photomultiplier tube are determined by the composition of the photocathode.
• The best photocathodes capable of responding to visible light are less than 30
percent quantum efficient, meaning that 70 percent of the photons impacting on
the photocathode do not produce a photoelectron and are therefore not detected.
• Photocathode thickness is an important variable that must be monitored to ensure
the proper response from absorbed photons.
• If the photocathode is too thick, more photons will be absorbed but fewer electrons
will be emitted from the back surface, but if it is too thin, too many photons will
pass through without being absorbed.
64. • Electrons emitted by the photocathode are
accelerated toward the dynode chain, which may
contain up to 14 elements.
• Focusing electrodes are usually present to ensure
that photoelectrons emitted near the edges of the
photocathode will be likely to land on the first
dynode.
• Upon impacting the first dynode, a photoelectron will
invoke the release of additional electron that are
accelerated toward the next dynode, and so on.
65. • The surface composition and geometry of the dynodes determines their ability to
serve as electron multipliers
• Because gain varies with the voltage across the dynodes and the total number of
dynodes, electron gains of 10 million are possible if 12-14 dynode stages are
employed.
• Photomultipliers produce a signal even in the absence of light due to dark current
arising from thermal emissions of electrons from the photocathode, leakage current
between dynodes, as well as stray high-energy radiation. Electronic noise also
contributes to the dark current and is often included in the dark-current value.
66. PHOTO DIODE
• A special type of PN junction device
that generates current when exposed
to light is known as Photodiode.
• It is also known as photodetector or
photosensor.
• It operates in reverse biased mode
and converts light energy into
electrical energy.
67. PRINCIPLE OF PHOTODIODE
• It works on the principle of
Photoelectric effect.
• The operating principle of the
photodiode is such that when the
junction of this two-terminal
semiconductor device is illuminated
then the electric current starts flowing
through it.
• Only minority current flows through
the device when the certain reverse
potential is applied to it.
68. CONSTRUCTION OF PHOTODIODE
• The PN junction of the device placed inside a glass material.
• This is done to order to allow the light energy to pass through it. As only the
junction is exposed to radiation, thus, the other portion of the glass material is
painted black or is metallised.
• The overall unit is of very small dimension nearly about 2.5 mm.
• It is noteworthy that the current flowing through the device is in micro-ampere and
is measured through an ammeter.
69. OPERATIONAL MODES OF PHOTODIODE
Photodiode basically operates in two modes:
Photovoltaic mode: It is also known as zero-bias mode because no external reverse
potential is provided to the device. However, the flow of minority carrier will take
place when the device is exposed to light.
Photoconductive mode: When a certain reverse potential is applied to the device
then it behaves as a photoconductive device. Here, an increase in depletion width is
seen with the corresponding change in reverse voltage.
71. • In the photodiode, a very small reverse current flows through the device that is
termed as dark current.
• It is called so because this current is totally the result of the flow of minority carriers
and is thus flows when the device is not exposed to radiation.
72. • The electrons present in the p side and holes present in n side are the minority
carriers.
• When a certain reverse-biased voltage is applied then minority carrier, holes from n-
side experiences repulsive force from the positive potential of the battery.
• Similarly, the electrons present in the p side experience repulsion from the negative
potential of the battery.
• Due to this movement electron and hole recombine at the junction resultantly
generating depletion region at the junction.
73. • Due to this movement, a very small reverse current flows through the device known as dark
current.
• The combination of electron and hole at the junction generates neutral atom at the
depletion. Due to which any further flow of current is restricted.
• Now, the junction of the device is illuminated with light. As the light falls on the surface of
the junction, then the temperature of the junction gets increased. This causes the electron
and hole to get separated from each other.
• At the two gets separated then electrons from n side gets attracted towards the positive
potential of the battery. Similarly, holes present in the p side get attracted to the negative
potential of the battery.
74. • This movement then generates high reverse current through the device.
• With the rise in the light intensity, more charge carriers are generated and flow
through the device. Thereby, producing a large electric current through the device.
• This current is then used to drive other circuits of the system
75. • The intensity of light energy is directly proportional to the current through the
device.
• Only positive biased potential can put the device in no current condition in case of
the photodiode
76. • Here, the vertical line represents the reverse
current flowing through the device and the
horizontal line represents the reverse-biased
potential.
• The first curve represents the dark current
that generates due to minority carriers in the
absence of light.
• all the curve shows almost equal spacing in
between them. This is so because current
proportionally increases with the luminous
flux.
78. ADVANTAGES OF
PHOTODIODE
It shows a quick response when
exposed to light.
Photodiode offers high operational
speed.
It provides a linear response.
It is a low-cost device.
79. DISADVANTAGES OF PHOTODIODE
It is a temperature-dependent device. And shows poor
temperature stability.
When low illumination is provided, then amplification is necessary.
80. APPLICATIONS OF PHOTODIODE
Photodiodes
majorly find its use
in counters and
switching circuits.
Photodiodes are
extensively used in
an optical
communication
system.
Logic circuits and
encoders also
make use of
photodiode.
It is widely used in
burglar alarm
systems.
82. HYBRID DETECTORS
• While its structure is similar to a conventional photomultiplier, the hybrid
photodetector (HPD) also has differences.
• Like PMTs, the hybrid is a vacuum tube with a photocathode that reacts to light, an
electron multiplier that multiplies electrons, and an output terminal that outputs an
electrical signal.
• But whereas PMTs use multiple dynodes as electron multipliers, the HPD uses a
silicon avalanche diode (AD) instead.
• This diode is composed of semiconductor layers: a thin layer of heavily doped p-
region that faces the photocathode and is connected to the output terminal, a much
thicker silicon substrate in the middle, and a p-n junction connected to a bias
terminal
83. • The HPD and PMT have different methods of multiplication.
• In a PMT, photoelectrons from the photocathode are accelerated by a voltage
difference towards the dynodes, where secondary electrons are generated from
dynode to dynode, yielding a signal gain of about 106.
• In an HPD, photoelectrons from the photocathode are accelerated toward the AD by
a larger voltage difference (about 8 kV).
• These photoelectrons are then multiplied in the AD in two steps: electron-
bombardment gain, followed by avalanche gain.
84. ELECTRON-BOMBARDMENT GAIN
• In electron-bombardment gain, each photoelectron deposits its kinetic energy in the
AD and produces many electron-hole pairs in the silicon substrate.
• The gain generated in this step depends on the voltage applied to the
photocathode—a voltage of–8 kV results in an electron-bombardment gain of about
1600.2
• The electrons then drift toward the p-n junction, and avalanche gain occurs.
85. AVALANCHE GAIN
• In avalanche gain, the electrons collide with the crystal lattice of the silicon and
create new electron-hole pairs that create more electron-hole pairs in a series of
chain reactions.
• Depending on the reverse-bias voltage applied to the AD, the avalanche gain can
range from 10 to 100.
• The total gain of the HPD is the product of the electron-bombardment and
avalanche gains and can be greater than 105.
86. VIDICONS
• Vidicon camera is a television camera which converts the light energy into electrical
energy.
• It functions on the principle of photo conductivity, where the resistance of target
material decreases when exposed to light.
87. CONSTRUCTION
• The Vidicon consists of a glass envelope with an optically flat face plate
• A photosensitive, target plate is available on the inner side of the face plate. The
target plate has two layers.
• To the front, facing the face plate, is a thin layer of tin oxide.
• This is transparent to light but electrically conductive.
• The other side of the target plate is coated with a semiconductor, photosensitive
antimony trisulphide. The tin oxide layer is connected to a power supply of 50V.
88. Grid-1 is the electron gun, consisting a cathode and a control grid.
The emitted electrons are accelerated by Grid-2.
The accelerated electrons are focussed on the photo conductive layer by Grid-
3.
Vertical and Horizontal deflecting coils, placed around the tube are used to
deflect the electron beam for scanning the target.
89. WORKING
• The light from a scene is focussed on the target.
• Light passes through the face plate and tin oxide, incident on the photo conductive
layer.
• Due to the variations in the light intensity of the scene, the resistance of the photo
conductive layer varies.
• The emitted electrons from antimony trisulphide reach the positive tin oxide layer.
90. CONTD..
• So, each point on the photo conductive layer acquires positive charge.
• Hence, a charge image that corresponds to the incident optical image is produced.
• As the electron beam from the gun is incident on the charge image, drop in voltage
takes place.
• As a result, a varying current is produced. This current produces the video-signal
output of the camera
91. PHOTODIODE ARRAY
Photodiode arrays (semiconductor devices) are used in the detection unit. A DAD
detects the absorption in UV to VIS region. While a UV-VIS detector has only one
sample-side light-receiving section, a DAD has multiple (1024 for L-2455/2455U)
photodiode arrays to obtain information over a wide range of wavelengths at one
time, which is a merit of the DAD.
92. CHARGE COUPLED DEVICE
• Charge coupled device (CCD) is an integrated circuit etched onto a silicon surface
forming light sensitive elements called pixels.
• Photons incident on this surface generate charge that can be read by electronics
and turned into a digital copy of the light patterns falling on the device.
• CCDs come in a wide variety of sizes and types and are used in many applications
from cell phone cameras to high-end scientific applications
93. CONTD..
• CCDs are 1D or 2D arrays of silicon PDs with metal–oxide semiconductor architectures.
• The detector arrays consist of individual detector elements, called pixels, which are defined by
capacitors, calledgates.
• Electrons generated by the light impinging onto the CCD charge these capacitors.
• Silicon exhibits an energy gap of 1.14 eV.
• Incoming photons with energy greater than this can excite valence electrons into the conduction
band, thus creating e–h pairs. The average lifetime for these carriers is 100 μs.
94. CONTD..
• After this time, the e–h pair will recombine. Photons with energy from 1.14 to 5 eV
generate single e–h pairs.
• Photons with energy of >5 eV produce multiple pairs.
• A 10 eV photon will produce 3 e–h pairs, on aver-age, for every incident photon.
• Soft x-ray photons can generate thousands of signal electrons, making it possible
for a CCD to detect single photons.
• For use as an IR imager, a CCD must be made of anothermaterial like germanium
(band gap 0.55 eV).
95. CONTD..
• The current sources (e–h pairs) produced are localized in small areas, an array of
capacitors, called pixels.
• Common 2D CCD chips have 512 × 512 or 1024 × 1024 pixels. The charge
accumulates in proportion to the light intensity impinging onto the pixel.
• A CCD sensor provides only one serial output, the readout register through which
each capacitor can be discharged (each pixel can be read).
• A differential voltage is applied across each gate to perform charge transfer.
96. CONTD..
• The photogenerated charge is moved to the readout register by a series of parallel shifts,
sequentially transferring charge from one pixel to the next within a column, until the charge
finally collects in the readout register.
• The charge from each row of pixels can be binned before readout to improve the S/N value.
• Furthermore, the dark count of CCDs is very low, especially when the detector is cooled.
• A CCD array can accumulate charge generated by photoelectrons almost noiselessly.
However, CCD noise is produced in the act of commutating the charge out to a charge
detector.
• Readout noise also tends to increase with increasing readout speeds; typically, the best CCD
camera systems currently available give around five electrons of readout noise per pixel.
97. CONTD..
• CCDs have several structures, including front-illuminated, back-illuminated, and
open-electrode structures .
• In front-illuminated CCDs, incident photons have to penetrate a polysilicon
electrode before reaching the depletion region. In back-illuminated or back-thinned
CCDs, the sub-strate is polished and thinned to remove most of the bulk silicon
substrate.
• Since illumination occurs from the back, the polysilicon on the front does not affect
the QE of the detector.
• CCDs are currently the detectors with the highest QEs.
• Typically, a CCD array has a QE of around 40%, but back-thinning can increase the
QE to around 80% at 600 nm.
98. CONTD..
• Back-thinned CCDs are usually coated with an antireflection material for enhanced
response in either the UV or the NIR region.
• Often, reflections from boundaries of the back-thinned devices form constructive
and destructive interference patterns, often referred to as the etaloning effect.
• The etaloning effect often causes an undesired oscillation superimposed on the
spectrum at wavelengths longer than 650 nm.
100. CONTD..
• The transmittance of the electrode depends on its thickness. Since the polysilicon
electrode material does not transmit below 400 nm, some versions of front-
illuminated UV CCDs have the detector coated with a phosphor, which converts UV
radiation to green light.
• These UV CCDs can provide a 10%–15% QE response in the 120–450 nm spectral
range.
• The coating is selected so that it does not degrade the visible and NIR response of
the detector.
• In open-electrode CCDs, the central area of the electrode is etched to expose the
underlying photosensitive silicon.
• These types of CCDs exhibit QEs of 30% or greater in the UV.
101. APPLICATIONS
Digital still and video cameras.
Astronomical telescopes, scanners, and bar code
readers.
Machine vision for robots, in optical character recognition
(OCR), in the processing of satellite photographs, and in the
enhancement of radar images, especially in meteorology.