1. The document discusses breeding objectives in chili pepper such as higher yield, resistance to biotic and abiotic stresses, and improved quality.
2. Case studies on breeding for resistance to diseases like anthracnose and viruses are presented. Sources of resistance to bacterial spot, Phytophthora root rot, and other stresses were identified.
3. Breeding methods discussed include pure line selection, pedigree breeding, backcrossing, and marker-assisted breeding. Achievements include new varieties with resistance to multiple stresses.
There is a huge demand for Mango worldwide. Hence, exporting to other countries we earn foreign currency. And, especially in Bangladesh, the suitable climate and soil condition is a positive sign of Mango cultivation. With the help of cut-edged technology here is a possibility of increased production.
“Advances in breeding of grapes ”
Advances breeding of Grape, breeding of grape, mutation breeding of grape, biotechnology breeding of grape ppt, breeding of grape by gangaram rana, Advances breeding of Grape in igkv ,
There is a huge demand for Mango worldwide. Hence, exporting to other countries we earn foreign currency. And, especially in Bangladesh, the suitable climate and soil condition is a positive sign of Mango cultivation. With the help of cut-edged technology here is a possibility of increased production.
“Advances in breeding of grapes ”
Advances breeding of Grape, breeding of grape, mutation breeding of grape, biotechnology breeding of grape ppt, breeding of grape by gangaram rana, Advances breeding of Grape in igkv ,
A review on Pharmacognosy of capsicum.
Headlines:-
Synonym
biological source
geographical source
cultivation and collection
macroscopical characterstics
capcicum oleo resin
method of preparation :- percolation
chemical constituents
chemical tests
uses
reference.
This presentation is about capsicum cultivation in Sri Lanka. Recommended varieties, Environmental Requirements, Nursery Management, Field Establishment, Crop Management, Pest & Disease Management, Harvest & Harvesting, Post Harvest Technology & Uses are included.
potato, caulilflower and cabbage breeding .pptxPremSidharthR
This presentation covers topics such as botanical description, Taxonomy, Breeding objectives, Breeding methods, problems and prospectus, and breeding stations related to potato breeding, Cauliflower breeding, Cabbage breeding.
This slides briefly summarize some of my research studies, which include studies on oomycetes, biological controls and plant growth-promoting bacteria, and pathogenic nematodes. The overall goal of the seminar was to show how our understanding of the various plant associating microbes can facilitate better crop production to ensure more food security.
Unit 2 plant tissue culture applications, advantages and limitationsDr. Mafatlal Kher
This presentation is related to the application of plant tissue culture techniques in various sectors, and it also highlights the advantages and limitations of plant tissue culture
Environmental condition (Temp. and R.H.) in storage and transit have also found to play important role in the development of post harvest diseases of the fruit crops; optimum temp. and relative humidity should maintained.
This study was carried out on the mycoflora associated with seeds of different citrus species. Citrus seed material was collected from districts of Punjab, i.e. Multan, Sargodha and Khanpur. Standard methods were applied for the isolation and identification of fungi. A total of 11 fungi including Aspergillus fumigatus, Aspergillus flavus, Dreschslera tetramera, Alternaria alternata, Curvularia lunata, Macrophomina phaseolina, Aspergillus niger, Fusarium solani, Fusarium moniliforme, Rhizopus and Penicillium spp were isolated from the seeds of citrus. For control of isolated seed-born fungi, 3 recommended fungicides such as Ridomil Gold, Bavistin, Score and two chemical Salicylic acid and Boric acid, were used at 20, 30, 40 mg/10 mL and 5, 6, 7 μL/10 mL, respectively and chemical with 20, 30, 40 mg/10 mL. All these fungicide and chemicals significantly reuced with population of all fungi present in naturally infected seed samples. Ridomil Gold and Salicylic acid were found to be the best for the control of se d-born fungi of citrus seed at 40 mg/10 mL. The isolation and identification of different mycotoxins is essential to study health status of the citrus consumers and to safeguard the standards of WTO.
"In field molecular diagnostics as an aid to disease management"EMPHASIS PROJECT
Insights about isothermal Polymerase Chain Reaction (PCR) assays and how they can be used to diagnose the presence of latent diseases in the field, including those which are especially difficult to identify. They will show how assays are developed, and how they may be used to improve disease management choices.
The target audience are researchers, agri-business and forestry experts, farmers and foresters and any other interested in plant health.
Do not hesitate to contact EMPHASIS project through Facebook, Twitter, email (emphasisproject@gmail.com) or through their website (http://www.emphasisproject.eu/) if you want to be updated on webinars dates and content and book a ticket.
To watch on Youtube: https://youtu.be/yFEG9uTEhdc
Grafting is a method employed to improve crop production. Grafting of vegetable seedlings is a unique horticultural technology practiced for many years in East Asia to overcome issues associated with intensive cultivation using limited arable land.The first grafted vegetable seedlings used were for Watermelon (Citrullus lanatus L.) plants grafted onto Lagenaria siceraria L. rootstock to overcome Fusarium wilt. Since then, the use of grafted solanaceous and cucurbitaceous seedlings has spread, with the practice mainly used in Asia, Europe, and North America. The expansion of grafting is likely due to its ability to provide tolerance to biotic stress, such as soilborne pathogens, and to abiotic stresses, such as cold, salinity, drought, and heavy metal toxicity, due to the resistance found in the rootstock. Many aspects related to rootstock/scion interactions are poorly understood, which can cause loss of fruit quality, reduced production, shorter postharvest time, and, most commonly, incompatibility between rootstock and scion. The rootstock and scion cultivars must be chosen with care to avoid loss.
The increased availability of biomedical data, particularly in the public domain, offers the opportunity to better understand human health and to develop effective therapeutics for a wide range of unmet medical needs. However, data scientists remain stymied by the fact that data remain hard to find and to productively reuse because data and their metadata i) are wholly inaccessible, ii) are in non-standard or incompatible representations, iii) do not conform to community standards, and iv) have unclear or highly restricted terms and conditions that preclude legitimate reuse. These limitations require a rethink on data can be made machine and AI-ready - the key motivation behind the FAIR Guiding Principles. Concurrently, while recent efforts have explored the use of deep learning to fuse disparate data into predictive models for a wide range of biomedical applications, these models often fail even when the correct answer is already known, and fail to explain individual predictions in terms that data scientists can appreciate. These limitations suggest that new methods to produce practical artificial intelligence are still needed.
In this talk, I will discuss our work in (1) building an integrative knowledge infrastructure to prepare FAIR and "AI-ready" data and services along with (2) neurosymbolic AI methods to improve the quality of predictions and to generate plausible explanations. Attention is given to standards, platforms, and methods to wrangle knowledge into simple, but effective semantic and latent representations, and to make these available into standards-compliant and discoverable interfaces that can be used in model building, validation, and explanation. Our work, and those of others in the field, creates a baseline for building trustworthy and easy to deploy AI models in biomedicine.
Bio
Dr. Michel Dumontier is the Distinguished Professor of Data Science at Maastricht University, founder and executive director of the Institute of Data Science, and co-founder of the FAIR (Findable, Accessible, Interoperable and Reusable) data principles. His research explores socio-technological approaches for responsible discovery science, which includes collaborative multi-modal knowledge graphs, privacy-preserving distributed data mining, and AI methods for drug discovery and personalized medicine. His work is supported through the Dutch National Research Agenda, the Netherlands Organisation for Scientific Research, Horizon Europe, the European Open Science Cloud, the US National Institutes of Health, and a Marie-Curie Innovative Training Network. He is the editor-in-chief for the journal Data Science and is internationally recognized for his contributions in bioinformatics, biomedical informatics, and semantic technologies including ontologies and linked data.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
3. • Important vegetable cum spice crop grown through out the world.
• Cultivated for green or dry fruit.
• ‘Capsicum’ = Greek word ‘kapto’, meaning "to bite" or "to swallow."
• Common names : hot pepper, sweet pepper, bell pepper,
ಮೆಣಸಿನಕಾಯಿ (in Kannada), paprika.
• Almost 400 types of chillis are grown through out theworld.
• It comprises numerous chemicals including steam volatile oils, fatty oils,
capsaicinoids, carotenoids, vitamins, proteins, fibres and mineral
elements (Bosland and Votava, 2000).
4. • An important part of dailydiet.
• Key Element in many regional cuisines,
pickles, soups, sauce, Salads, curries
etc. due to its unique flavor, aroma and
colour.
• Increase the taste and palatability.
• Fresh green capsicum contain more
vitamin C than citrus fruits and fresh
red chilli has more vitamin A than
carrot. (Than et al. 2008).
• Chillies are low in sodium and
cholesterolfree.
• medicinal Properties are found…
• stimulate blood
circulation
• improves the digestion
process
• rich source of
antioxidants
• source of natural
bactericidal agents
• Apart from medicinal uses chilli
also used in cosmetic , liquor
industries and as a weapon for
self defense (chilli spray).
7. • Kingdom
• Class
• Order
• Family
• Genus
• Species
• Scientificname
• Chromosome no.
: Plantae
: Angiosperm
: Solanales
: Solanaceae
: Capsicum
: annum
: Capsicum annumL.,
var. annum
var. paprika
: 2n=24 & 2n=26
Scientific Classification
8. Floral morphology
Flower: actinomorphic, bisexual, pedicellate,hypogynous
Gynoecium: Bicarpellary, syncarpous, bilocular or tetralocular
due to pseudoseptum. Many ovules on axile placentation, ovary
superior.
Androecium: Stamens 5, epipetalous
Self pollinated crop.
Flowers open at5 a.m.
Receptivity of stigma : from a day earlier to anthesis and up to 2
days after anthesis.
Fertility of pollen : a day before anthesis with maximum fertility
on the day of anthesis.
Emasculation and Pollination on the sameday.
Percent fruit set : 20% of the flowersproduced.
Isolation distance : 500m.
Hand emasculation and pollination is most common method
used for hybrid seed production in chilli.
9. Crossing technique
A bud one day prior to anthesis should beselected
for emasculation
Emasculation should be carried out without
damaging female reproductive parts and bagged it
At a same time pollen should be collectedfrom the
fully opened male flower
Gently transfer the pollen to the stigma , bagging
and tagging should be done simultaneously
10. Breeding objectives in Capsicumspp.
Major
1. Higheryield
2. More pungency (capsaicin) and oleoresin in hot chilli
3. Zero pungency (capsaicin) and more antioxidant in sweetpepper
4. Resistance to anthracnose, virus complex, fruit rot and bacterial
wiltetc.
5. More dry powder from green fruit
Minor
1. More number of fruits per plant
2. Higher fruit weightand larger size
3. Uniform fruit shape, size and color in sweetpepper
4. Earliness
5. Wideradaptability
6. Improved nutritional quality
7. Longer shelflife
11. 1) Introduction
2) Pure line selection
3) Pedigreemethod
4) Backcross method
5) Heterosisbreeding
6) Distancehybridization
1) Markerassisted
breeding
2) Tissueculture
3) Mutationbreeding
Conventional Non conventional
Breeding strategy / methods
12.
13. DIVERSITY, CONSERVATION, AND ENHANCEMENT OF
GENETIC RESOURCES
non-hierarchical clustering
based on Mahalanobis D2 values
among 30 chilli genotypes and
grouped them into six clusters
Analysis of variance, cluster
analysis based on Tocher’s method
was performed using the statistical
software Indostat and statistical
package for agricultural research
(SPAR) version 2.0 programme.
The National Bureau of Plant Genetic Resources (NBPGR), New Delhi facilitates
collection, regeneration, characterization, conservation and distribution of chilli
germplasm to researchers in India. However, at NBPGR indigenous collections constitute
only 18% of the total Capsicum collections, while the majority of the
accessions are exotics (Kalloo et al., 2005).
15. BREEDING FOR RESISTANCE TO BIOTIC AND ABIOTIC STRESSESBREEDING FOR RESISTANCE TO BIOTIC AND ABIOTIC STRESSES
Causal Organism : Tobacco Mosaic Virus
Transmitted by : whitefly
Mosaic patterns consisting ofbands of light
green or yellow alternating with dark green.
Sometimes the leaves are crinkled or distortion.
Plant growth is stunted.
Causal Organism : Colletotrichum spp.
Infection occurs during periods of excess irrigation
or rain
Small, watersoaked lesions that expand rapidly.
Fully expanded lesions are sunken and range in
color from dark red to black in concentric rings
C. O. : Alternaria spp.
• Dusty black spot on
fruit and leaves
• Fruit rot
C. O. : Phytoptheracapsici
• Dark lesions of the stem
• Blighting of plant
• Water soaked gray spot
on fruit & it mummifies
57
16. Bacterial spot caused by Xanthomonas campestris pv.
vesicatoria is causing a significant damage on chilli pepper
New sources of resistance to bacterial spot found in
Vietnam, Laos and Nepal collections were crossed to a
cytoplasmic male sterile line (Srfrf), Chilbok-A, which has
been bred by incorporation of Phytophthora resistance of
CM334 into a local cultivar
17. evaluated the types of gene action governing the inheritance of resistance to
Phytophthora nicotianae necrosis in populations derived from two crosses
18. Phytophthora capsici is a soilborne fungal pathogen that can
cause four different disease syndromes in Capsicum known as root
rot, foliar blight, stem blight, and pod rot.
The accession “Criollo de Morelos 334” (Capsicum annuum) is
the most stable resistance source
incorporated P. capsici root rot resistance from “CM 334” to the
“Orange Habanero” accession (C. chinense) with the backcross
breeding method.
19.
20.
21. Variety
% Disease suseptibility
Date after Inoculation Field Resistant Evaluation
7 14 21 61
Wonkyo 306 0 0 0 0.03 R R
Shinhong 5 23 32 75 M M
Cheonanjaelae 10 70 70 95 S MS
Table : Reaction of Wonkyo 306 ToPhytophthora capsici.
Table : Reaction of Wonkyo 306 to Virus, Anthracnose and Bacterial Leaf Spot.
Variety Virus Anthracnose Bacterial Leaf Spot
Wonkyo 306 R MR MR
Shinhong S MS MS
Cheonan S M S
Kwan et al. (2014)Korea
Three way F1 'wonkyo 306' with the multi resistance in Capsicum annuum
Taen jaelae (F1) Gimjanggochu
three way F1
WONKYO 306 (F6)
Selfed & subsequent selection
X
(local selection)
Resistance to
TMV, AnthracnoseGood yielding,
Susceptible to
Bacterial LS
22. Disease/insect /stress Resistant/tolerant source
Fruit rot (Colletotrichum sp.) C. chinense, Accr 1555, 1554, 906, Chinese Giant,
Hungarian Yellow Wax, Spartan
Cercospora leaf spot California Wonder, Hungarian Wax
Bacterial leaf spot PI 163192, PI 260435, PI 163189, PI 163192, PI
271322, PI 32219
Phytophthora root rot PI 201234
Phytophthoranicotianae PBG 631, UHF-1
Root knot nematode Santaba
TMV Sonnette, Keystone Resistant Giant, YW, Yolo Y
Tolerant to drought Arka Lohit
Tolerant to salinity PLR 1
Resistant / Tolerant sources
23. BREEDING FOR QUALITY
assess the stability of quality traits in stored chilli powder.
The red ripe fruits of eight chilli genotypes (Capsicum annuum
L) were evaluated for quality parameters viz. capsaicin,
extractable colour and colour value in freshly grinded powder as
well as in powder stored at ambient temperature for six months.
24. presence or absent of pungency in pepper is inherited as a
monogenic trait controlled by the dominant Pun1 gene, variation in
capsaicinoids content among pungent cultivars is inherited as a
quantitative trait.
a major quantitativetrait locus (QTL) identified to present in
chromosome 7, termed cap, that controls capsaicinoids content
detected a second more minor QTL in chromosome 8
25. BREEDING FOR YIELD
Molecular markers of the Quantitative Trait Loci (QTLs) for yield-
related traits and for model parameters are needed for phenotype
prediction.
To improve the estimation of allelic values at QTLs, functional
markers (sequence polymorphism controlling the phenotypic
variation) are expected instead of QTL flanking markers.
The microarray technology is widely used in
gene expression studies.
26. The prediction of phenotypic responses from genetic and
environmental information is an area of active research in
genetics, physiology and statistics.
A wide array of -omics data can be generated linking genotype
and phenotype.
27. Ten chilli varieties including checks (PC-2062, ACS-06-01, ACS-06-02,
CCH-05-01, AKC-406, BCC-1, VR-378, LCA-206, JCA-283, Byadagi Kaddi)
were used.
Observations on days to 50% flowering, plant height, branches per
plant, number of fruits and yield per plant, fruit length, fruit
width/diameter, fruit weight, green fruit yield per hectare based on
plot yield was recorded and statistically analyzed.
These ten varieties differed significantly for all the traits except plant
height.
28.
29. DEVELOPMENT OF MOLECULAR AND OTHER
BIOTECHNOLOGICAL TOOLS
Nuclear ms8 gene is a single recessive gene which can be used
to develop the male sterility system, applicable in sweet pepper
hybrid seed production.
Such a nuclear male sterility system would be more effective
if molecular markers of ms8 gene were available.
RAPD and DArT technologies combined with BSA (Bulked
Segregant Analysis) were used to identify molecular markers of
ms8 gene.
DArT BSA analysis resulted in the identification of seven DArT
markers potentially linked to ms8 locus.
32. High variability present in nature for quality and yield
contributing characters.
Characters like fruits per plant, pricarp thickness, fruit size, fruit
weight and oleoresin content can be used for selection.
Heterosis can be manifested using diverse germplasm.
Easy and more hybrid seed production can be possible through
proper exploitation of male sterility system.
Mutation can be employed to create new useful mutant for
crop improvement.
In vitro cloning is more successful when cotyledon leaf was
used as an explants.
Abiotic stress tolerant germplasm present in nature.
Disease resistant cultivar can be developed through crossing
and backcrossing with resistance germplasm.
33. Genesis of varieties with less pungency and consumerpreference.
Exploitation of male sterility and chemical hybridizing agents in
developing new hybrids.
Introduction of heat and drought tolerance germplasm as a strategy for
climate change.
Development of location specific varieties.
Todevelop a varieties which can maintain as such capsaicin content
even after a longer storage period.
Developments of varieties with higher antioxident and oleoresin
content.
Breeding for ornamental plant type.