This document provides information on the history, breeding methods, and genetic resources of banana. It discusses how banana breeding was started in Trinidad and Jamaica in the 1920s to develop Fusarium wilt resistant varieties. Important breeding programs and achievements are highlighted from India, including the development of hybrid varieties BRS-1 and BRS-2 at Kerala Agricultural University. Mutation breeding has also led to new varieties. The objectives of banana breeding include developing dwarf, disease resistant varieties with good quality fruit suited for different agro-ecological zones.
“Advances in breeding of guava ”
Advances breeding of Guava, breeding of guava by gangaram rana, breeding of guava in igkv, cultivation of guava, new technology of guava breeding, poly ploide breeding of guava, mutation breeding of guava
“Advances in breeding of guava ”
Advances breeding of Guava, breeding of guava by gangaram rana, breeding of guava in igkv, cultivation of guava, new technology of guava breeding, poly ploide breeding of guava, mutation breeding of guava
“Advances in breeding of mango ”
Advances breeding of Mango, breeding of mango, mutation breeding og mango ,breeding of mango by gangaram rana ppt , breeding of mango in igkv
The pineapple is a tropical and subtropical fruit .
Pineapple (Ananas comosus) is one of the commercially important fruit crops of India.
Total annual world production is estimated at 14.6 MT of fruits.
India is the fifth largest producer of pineapple with an annual output of about 1.2 MT.
common throughout the Bromeliaceae but Ananus is typically diploid (2n=2X=50)while pseudananas is tetraploid (2n=4X=100).Some triplod genotype with 2n=3x=75(e.g. Cayenne BR59,Spanish GU75-2 and DOS indios BR47)have also reported to exist.
Other leading producers are Thailand, Philippines, Brazil, China, Nigeria, Mexico, Indonesia, Colombia and USA.Cultivation of pineapple originated in Brazil.
Since litchi originated in China and it has been under cultivation there for more than 2200 years, more than 200 litchi varieties exist in China.
The variation in climatic factors, sometimes leads to greater fluctuation in yield of a litchi orchard.
Therefore, a right variety should be selected for plantation at a particular area though, all the litchi varieties have a wide range of adaptability; yield, fruit quality and acceptability may be region or location specific.
There is a huge demand for Mango worldwide. Hence, exporting to other countries we earn foreign currency. And, especially in Bangladesh, the suitable climate and soil condition is a positive sign of Mango cultivation. With the help of cut-edged technology here is a possibility of increased production.
“Advances in breeding of grapes ”
Advances breeding of Grape, breeding of grape, mutation breeding of grape, biotechnology breeding of grape ppt, breeding of grape by gangaram rana, Advances breeding of Grape in igkv ,
Origin, distribution, area & production, taxonomy, floral biology, varieties, and species, objective of breeding in different countries and major breeding programmes, approaches and achivements
“Advances in breeding of banana ”
Advances breeding of Banana, breeding of banana , breeding of banana by gangaram rana, Advances breeding of Banana igkv, mutation breeding of banana, biotechnology of banana, seed production breeding of banana,
“Advances in breeding of mango ”
Advances breeding of Mango, breeding of mango, mutation breeding og mango ,breeding of mango by gangaram rana ppt , breeding of mango in igkv
The pineapple is a tropical and subtropical fruit .
Pineapple (Ananas comosus) is one of the commercially important fruit crops of India.
Total annual world production is estimated at 14.6 MT of fruits.
India is the fifth largest producer of pineapple with an annual output of about 1.2 MT.
common throughout the Bromeliaceae but Ananus is typically diploid (2n=2X=50)while pseudananas is tetraploid (2n=4X=100).Some triplod genotype with 2n=3x=75(e.g. Cayenne BR59,Spanish GU75-2 and DOS indios BR47)have also reported to exist.
Other leading producers are Thailand, Philippines, Brazil, China, Nigeria, Mexico, Indonesia, Colombia and USA.Cultivation of pineapple originated in Brazil.
Since litchi originated in China and it has been under cultivation there for more than 2200 years, more than 200 litchi varieties exist in China.
The variation in climatic factors, sometimes leads to greater fluctuation in yield of a litchi orchard.
Therefore, a right variety should be selected for plantation at a particular area though, all the litchi varieties have a wide range of adaptability; yield, fruit quality and acceptability may be region or location specific.
There is a huge demand for Mango worldwide. Hence, exporting to other countries we earn foreign currency. And, especially in Bangladesh, the suitable climate and soil condition is a positive sign of Mango cultivation. With the help of cut-edged technology here is a possibility of increased production.
“Advances in breeding of grapes ”
Advances breeding of Grape, breeding of grape, mutation breeding of grape, biotechnology breeding of grape ppt, breeding of grape by gangaram rana, Advances breeding of Grape in igkv ,
Origin, distribution, area & production, taxonomy, floral biology, varieties, and species, objective of breeding in different countries and major breeding programmes, approaches and achivements
“Advances in breeding of banana ”
Advances breeding of Banana, breeding of banana , breeding of banana by gangaram rana, Advances breeding of Banana igkv, mutation breeding of banana, biotechnology of banana, seed production breeding of banana,
Okra breeding methods, objectives and important varietiesVikraman A
In this presentation I given information about okra crop for origin and distribution, breeding objectives and methods and some important varieties with description.
Banana breeding was started in Trinidad, West Indies in 1922 and in Jamaica in 1924.
The driving force for this breeding programme was to develop improved Fusarium wilt (Fusarium oxsyorum F.sp. Cubense) resistant banana for export trade.
In 1960, both the programmes were combined under the Jamaica Banana Board.
United Fruit Company also started a small breeding programme in Panama in 1920s.
In India hybridization work was started at Central Banana Research Station, Adhuthurai, Tamil Nadu in 1949.
Presentation at the November 2012 dialogue workshop of the Biosciences for Farming in Africa media fellowship programme in Arusha, Tanzania.
Please see www.b4fa.org for more information
GPB 311: Wheat- Centre of origin, distribution of species, wild relatives and major breeding objectives and procedures for development of varieties and hybrids for improvement yield, adoptability, stability, biotic and abiotic stress tolerance and quality in Wheat
A mango is a juicy stone fruit produced from numerous species of tropical trees belonging to the flowering plant genus Mangifera, cultivated mostly for their edible fruit. Most of these species are found in nature as wild mangoes. The genus belongs to the cashew family Anacardiaceae.
Germplasm Conservation, Evaluation, Documentation, Distribution and Quaranti...VanangamudiK1
Germplasms Conservation, Evaluation, Documentation, Distribution and Quarantine
Plant Breeding
K. Vanangamudi
Field seed bank
Shoot tip bank
DNA banks
International Institutes conserving germplasm
Study in respect to origin distribution of species –wild relatives- and forms of breeding objectives –major breeding procedure for development of hybrids varieties in wheat
Embryo culture is a laboratory method for producing plant lets from a fertilized or unfertilized embryo in invitro condition. there are several advantages are associated with the embryo culture like production of haploid plants, making distant crosses successful, sometimes aborted embryos can be rescued from a unsuccessful hybridization.
The main objective of anther culture is production of haploid plants, which are useful in rapid production of homozygous lines and also production of doubled haploids (DH). Anther culture or pollen culture provides certain advantages over conventional breeding strategies like production of homozygous lines which takes 5-7 generations in case of conventional breeding strategies.
The Yamada et al., and Guha and Ramachandra are first to produce the haploid plants through anther culture in 1936 and 1964 respectively.
N6 is the popular media for used for anther culture.
This presentation deals with procedure, applications, limitations associated with the Anther culture.
General steps in biotechnology: and Various sterilization techniques followed in a tissue culture lab space, such as autoclaving, filtering, flame sterilization, chemical sterilization, UV radiation etc.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
2. •Botanical name: Musa sp.
•Family : Musaceae
•Chromosome number: n=11
2n = 22, 33 or 44.
•Origin: South East Asia
3. • History of banana breeding:
• Banana breeding was started in Trinidad, West Indies in
1922 and in Jamaica in 1924.
• The driving force for this breeding programme was to
develop improved Fusarium wilt (Fusarium oxsyorum
F.sp. Cubense) resistant banana for export trade.
• In 1960, both the programmes were combined under
the Jamaica Banana Board.
• United Fruit Company also started a small breeding
programme in Panama in 1920s.
• In India hybridization work was started at Central
Banana Research Station, Adhuthurai, Tamil Nadu in
1949.
• Important banana growing states are Maharashtra,
Karnataka, Kerala, Tamil Nadu, Andhra Pradesh,
Orissa,Bihar, West Bengal and Assam .
4. • In South India, other than its edible use, banana is
extensively used in all auspicious occasions such as
wedding, festivals and worshipping God.
• Banana is a good table fruit, besides, the cultivar
Nendran is used for cooking.
• Centre of diversity:
• Edible banana is native to old world especially South
East Asia (Simmonds, 1962).
• Malayan area seems to be the primary center of origin
of cultivated banana
• (M.acuminata). M.acuminata, was probably introduced
into India and Burma where M.balbisiana is a native
species.
• Natural hybridization between these two species might
have resulted in many hybrid progenies (AAB, ABB etc.).
5. • Genetic resources
• Musa has about 50 species and this genus is divided
into five sections:
• a) Eumusa: Includes about 13-15 species of edible and
wild banana. The chromosome number is 2n=22 in wild
species and most of the cultivated varieties are having
2n=33 (2n=44 rarely) e.g. M.acuminata, M.balbisiana,
M.basjoo etc.
• b) Rhodochlamys: Mostly diploid, spread from India to
Indonesia. Five to seven species are kept in this group.
Parthenocarpy is absent in this group e.g. M.ornata,
M.velutina.
• c) Callimusa: This is of ornamental value and x=10 and
2n =20. It is found in Indo-China, Malaya and Borneo.
Parthenocarpy is absent in this type. It includes about
5-6 species e.g. M.coccinea.
6. • d) Australimusa: Like Callimusa it has x = 10 and
2n=20 chromosome. Species of this group is
common in Queensland and Philippines. Important
species of this group are M. textilis or manilahemp,
M.maclavi etc.
• e) Incertae sedis: It includes M.ingens (x=7, 2n=14)
of New Guinea which grows to a height of over 10
m. This is the largest known herb. Another species
in this group is M.beccarii (x=9, 2n=18) from North
Borneo.
7. • The most important Musa cultivars are almost
sterile triploids (2n=3x=33) and also tetraploid and
diploid banana cultivars have also local importance
in Asia.
• All banana and plantain land races are farmers
selection from intra and inter specific hybridization
of two different species.
• M.acuminata Colta, donor of the A genome and
M.balbisiana Colta, donor of the B genome.
• Simmonds and Shepherd (1955) reported scoring
technique to indicate the relative contribution of
the two wild species for the constitution of a given
cultivar.
• Fifteen distinguishing characters between Musa
acuminata and Musa balbisiana were identified by
them.
8. • At the botanical garden, Howrah, seeds of few banana
species were collected from Chittagong and Madras.
• More number of genotypes of banana was also
maintained at Central Banana Research Station,
Aduthurai.
• After that it was shifted to Horticulture college and
research Institute, Tamil Nadu Agricultural University,
Coimbatore.
• After the formation of National Research Centre on
Banana (NRCB) in 1995, a wide germplasm collection
including wild types are being maintained at this center
and intensive research programmes are being taken up
on various problems related with banana.
• Presently, TNAU also maintaining 186 collections of
germplasm.
9. • Taxonomic classification of edible banana
(Simmonds and Shepherd, 1955)
Genome Ploidy level Nomenclature
AA 2x Matti, Anai komban
AAA 3x
Cavendish, Robusta,
Grand Nine
AAAA 4x
Bodles Altafort
(Synthetic
hybrid of West Indies)
AB 2x Ney Poovan, Kunnan
AAB
3x
Poovan, Hill banana,
Champa, Rsathali
ABB
3x Monthan, Kanchkela,
ABBB
4x Klue Teparod
10. • Objectives of breeding
• To develop dwarf statured banana suitable for high
density planting and to prevent damage from high wind
velocity.
• Production of good quality fruits.
• Resistant to biotic and biotic stresses i.e. nematodes,
panama wilt, bunchy top, sigatoka leaf spot, moko
disease and pseudo stem weevil etc.
• To develop varieties with wider agro-ecological
adaptability.
• Development of male fertile parthenocarpic diploids
with resistance to major diseases and pests.
• Developing longer finger size.
• Suitability for export.
• Good keeping quality.
11. • Breeding methods and achievements:
• Introduction.
• Hybridization
• Mutation breeding
• Breeding works of different areas
12. • Introduction
• Introduction of some cultivators of banana was
made with resistance to biotic stresses e.g. Lady
Finger (EC 160160) resistant to bunchy top virus
introduced from Australia and is being evaluated at
IIHR, Bangalore and TNAU, Coimbatore.
• Further, cultivars Naine MS (EC 27237) from France
and Valery from West Indies were introduced for
utilization in improvement programme
13. • Hybridization
• In India, breeding work was started at Central
Banana Research Station, Aduthurai (Tamil Nadu) in
1949.
• Technique of hybridization in banana is different
from other crops.
• Pollination is best carried out in the morning. The
bunches of female parent are bagged at shooting
and each successive hand is pollinated as it is
exposed. At maturity and ripening the bunch is cut
and seeds are extracted. Seeds are sown at once in
the green house.
14. • Evaluation of hybrid progenies from seedlings to
harvest may not be the correct phase instead,
evaluation of the same under next vegetative phase
i.e., sucker to harvest stage will be ideal as full
expression of yield potential could be observed
only in the second crop of the F1 progeny.
• The first crop (seedling to harvest) takes more than
15-19 months, where most of the energy of the
plants is needed for corm formation.
16. • At Kerala Agricultural University, two hybrids viz.,
• BRS-1 (Agniswar x Pisang lilin) and
• BRS -2 (Vannan x Pisang lillin) have been
developed.
• BRS -1 (AAB) is 100 days earlier than Rasthali with
significant differences in bunch weight. It has been
released for homestead cultivation in Kerala, as it is
resistant to sigatoka leaf spot.
• BRS-2 (AAB) is a medium statured hybrid, tolerant
to leaf spot and panama disease, rhizome, weevil
and nematodes. The average bunch weight is 14 kg
with 8 hands and 118 fruits crop duration of 314
days.
17. • Breeding work in other Countries:
• PITA-9: A Black Sigatoka Resistant (BSR) hybrid from
the “False Horn” plantain, a tetraploid hybrid
having black Sigatoka resistance has been
developed at International Institute of Tropical
Agriculture (IITA), Nigeria.
• ‘BITA-3’ is a tetraploid starchy banana hybrid with
low partial resistance to black Sigatoka disease
developed at IITA High Rainfall Station in Onne
(Southeastern Nigeria), where both (Banana streak
virus) and cucumber mosaic virus (CMV) have been
observed.
• ‘BITA-3’ is a hybrid from the interspecific cross
‘Laknau’ x ‘Taju Lagada’, ‘Laknau’ is a female –fertile
AAB starchy banana that closely resembles
plantains.
18. • Mutation breeding:
• Bud mutation in Indian banana is very common
perhaps due to spontaneous rearrangement of
chromosomes in somatic meristem and structural
re-assortment.
• High gate (AAA) is a semi-dwarf mutant of Gros
Michel (AAA),
• Motta Poovan (AAB) is a sport of Poovan (AAB),
• Ayiranka Rasthali a sport of Rasthali (or Silk),
• Barhari Malbhog is a sport of Malbhog,
• Krishna Vazhai is a natural mutant of Virupakshi (or
Pome),
• Sambrani Monthan (ABB), a mutant of Monthan
(ABB).
19.
20. Name of the clone/cultivars Name of the biotic and abiotic stress
Musa balbisiana
Calcutta-4
Pisang Lilin
SH3142 (Diploid hybrid)
Musa acuminata sp malaccensis
Musa acuminata sp burmannica
Pisang Jari Buaya (PJB)
Tongat and Anaikomban
Drought
Black sigatoka
Panama wilt (Race1)
Race 1 of Fusarium
Race 1 and Race 2 of Fusarium
Bacterial wilt race 2, Moko
Disease
Burrowing nematode
Nematodes