Creative Biolabs offers DNA immunization services to generate polyclonal and monoclonal antibodies. DNA immunization allows for in vivo production of antigens with proper post-translational modifications to induce a strong immune response. DNA immunization elicits an immune reaction against a foreign protein by permitting in vivo antigen production, bypassing difficult protein synthesis and purification steps. The expressed proteins maintain their native structure and post-translational modifications, allowing for antibodies against the natural target conformation. DNA immunization can also be used to develop antibodies against challenging antigens like membrane or toxic proteins. Creative Biolabs' DNA immunization strategies include gene gun delivery, electroporation, naked DNA, and heterologous immunization to meet project objectives.

1. genetic immunization
• Creative Biolabs is proud to offer the innovative DNA immunization service to customize polyclonal and monoclonal
antibodies. As an advanced strategy, DNA immunization enables the immunized hosts to in vivo produce interested
antigens with common post-transcriptional modifications and then induce a powerful immune response.
• What is DNA immunization?
• DNA immunization is one of the most straight-forward approaches to elicit an immune reaction against a foreign
protein. Differ from the conventional immunization methods, DNA immunization permits in vivo antigen
production, bypassing the time-consuming (sometimes also difficult) processes of immunogens (e.g. peptides and
recombinant proteins) synthesis and purification. In addition, these in vivo expressed proteins can maximally
maintain the naïve structures and go through appropriate post-transcriptional modifications. These properties can
contribute to the generation of high-affinity antibodies against the natural conformation of the target antigen,
which is a crucial feature for developing therapeutic antibodies. Meanwhile, this approach is also an ideal solution
to develop antibodies against membrane proteins or other challenging or problematic antigens. Furthermore, DNA
immunization is also a novel tool in the early phases of DNA vaccine development.

2. • Challenging or Problematic Antigens Available for DNA Immunization
• Multi-pass membrane proteins (i.e. GPCRs and ion channels)
• Insoluble proteins
• PTM proteins
• Unstable proteins
• Intracellular proteins
• Large protein domains
• Secreted proteins
• Proteins containing disulfide bonds
• Toxic proteins
• Unknown proteins whose genes have been obtained
• With years of research and development experience, Creative Biolabs can offer a series of DNA immunization strategies to meet our clients’
specific project objectives. Our featured strategies including but not limited to:

3. • Magic™ DNA Immunization Strategy
• Gene Gun DNA Immunization Strategy
• In vivo Electroporation DNA Immunization Strategy
• Naked DNA Immunization Strategy
• Heterologous Immunization Strategy
• On the basis of the provided sequencing information, our experienced scientists could offer one-stop service from gene synthesis to immunization, and then generate high quality
polyclonal or monoclonal antibodies by one of our antibody discovery technologies:
• The conceptual graphs of DNA immunization. Through DNA immunization, the immune vectors contain the interested DNA are delivered into the living cells. The <em>in
vivo</em> expressed foreign proteins are then processed and presented <em>via</em> MHC pathway to alert the immune system.
• Antibody Library Construction and Screening via DNA Immunization
• Native™ Antibody Discovery (B-cell Sorting) via DNA Immunization
• Hybridoma Production via DNA Immunization
• Polyclonal Antibody Production via DNA Immunization

4. • Additional Advantages of DNA Immunization
• Only requires in silico DNA sequence
• No need recombinant protein or peptide
• No risk of antigen contamination
• Time- and labor-saving
• Generate antibody with high affinity and specificity
• In our Magic™ DNA immunization service, the Magic™ DNA transfection reagent is employed to transfect the
immune plasmid into cell line for the immune response validation and further screening. Furthermore, Creative
Biolabs can also provide a series of specific Magic™ transfection reagents as individual products for our clients.
These Magic™ reagents can achieve high efficiency in vitro transfection of either DNA, mRNA or siRNA for a broad
range of cell types of cell lines to assist our clients’ research and project development.