- Biotechnology uses living organisms or enzymes from organisms to produce useful products for humans. Modern biotechnology is based on genetic engineering and maintaining sterile conditions.
- Genetic engineering alters genetic material (DNA and RNA) to introduce it into host organisms and change their phenotype. This allows isolation of only desirable genes without undesirable genes.
- Recombinant DNA technology involves isolating DNA, cutting it with restriction enzymes, ligating DNA fragments into vectors, transforming hosts, and extracting the desired product.
This presentation gives brief introduction of Recombinant DNA technology. This presentation covers steps involved and tools of Rec DNA Technology. important applications are also explained in this presentation.
This presentation gives brief introduction of Recombinant DNA technology. This presentation covers steps involved and tools of Rec DNA Technology. important applications are also explained in this presentation.
A power point presentation on the biology topic of "Recombinant DNA Technology" based on class 12 CBSE boards practical topics .It's contain a basic description and a possible explanation for a better understanding.
Study of cloning vectors and recombinant dna technologySteffi Thomas
Study of cloning vectors, restriction endonuclease and DNA ligase, Recombinant DNA technology, Application of genetic engineering in medicine, Application of rDNA technology and genetic engineering in the production of interferons, Vaccines-hepatitis-B, Hormones-Insulin, Brief introduction to PCR
To describe DNA extraction
To explain and demonstrate DNA cloning
To explain the process of PCR and its uses.
To explain DNA fingerprinting and its uses
Genetic engineering principle, tools, techniques, types and applicationTarun Kapoor
Basic principles of genetic engineering.
Study of cloning vectors, restriction endonucleases and DNA ligase.
Recombinant DNA technology. Application of genetic engineering in medicine.
Application of r DNA technology and genetic engineering in the products:
a. Interferon
b. Vaccines- hepatitis- B
c. Hormones- Insulin.
Polymerase chain reaction
Brief introduction to PCR
Basic principles of PCR
Genetic Engineering By Ghawas khan from AWKUM PharmacyGhawas
Genetic Enginnearing is on one of the emerging branch of life sciences... Tried my best to create a best presentation for you. Remember me in your prayers.
A power point presentation on the biology topic of "Recombinant DNA Technology" based on class 12 CBSE boards practical topics .It's contain a basic description and a possible explanation for a better understanding.
Study of cloning vectors and recombinant dna technologySteffi Thomas
Study of cloning vectors, restriction endonuclease and DNA ligase, Recombinant DNA technology, Application of genetic engineering in medicine, Application of rDNA technology and genetic engineering in the production of interferons, Vaccines-hepatitis-B, Hormones-Insulin, Brief introduction to PCR
To describe DNA extraction
To explain and demonstrate DNA cloning
To explain the process of PCR and its uses.
To explain DNA fingerprinting and its uses
Genetic engineering principle, tools, techniques, types and applicationTarun Kapoor
Basic principles of genetic engineering.
Study of cloning vectors, restriction endonucleases and DNA ligase.
Recombinant DNA technology. Application of genetic engineering in medicine.
Application of r DNA technology and genetic engineering in the products:
a. Interferon
b. Vaccines- hepatitis- B
c. Hormones- Insulin.
Polymerase chain reaction
Brief introduction to PCR
Basic principles of PCR
Genetic Engineering By Ghawas khan from AWKUM PharmacyGhawas
Genetic Enginnearing is on one of the emerging branch of life sciences... Tried my best to create a best presentation for you. Remember me in your prayers.
Mind-Control-Foltermorde, Staatsspionage, Repressionen oder Unfähigkeit des S...Oliver Grell
Mind-Control-Foltermorde, Stasi-Seilschaften, Psychopolitik und Psychokrieg,
Mind Control, Folterungen, Verstümmelungen, Morde, Terrorismus und mundtot machen in der BRD. Repressionen!
Das geht gar nicht!
Über Jahrzehnte, 10, 15 und über 20 Jahre, werden Menschen langsam zutode gefoltert. Wer in dessen Umfeld etwas mitbekommt, wird fertig gemacht. Wacht auf. Illegale Menschenversuche, Nazi-Eugenik, Spionage in allen Bereichen und äquivalente Verbrechen an Menschen grassieren wie Vieren. Da wird etwas vorgeschoben um rücksichtslos Menschen für kriminelle Machenschaften zu ermorden. Nicht irgendwo, sondern in unserer BRD. Ohne staatliche Beteiligung wird das kaum möglich sein.
Was ist ein Foltermord! "Das ist, im Gegensatz zur Menschenabschlachtung, das nicht zeitlich begrenzte Schlachten eines Menschen, unter dem Motto "Der Weg ist das Ziel" und soll einem Menschen den größt möglichen Schaden über die größt mögliche Zeit zufügen".
Ein Begriff, der durch die e-Waffen und Mind Control erst jetzt im 21 Jahrhundert geprägt wird und definiert werden sollte. Eine Aufnahme in den Duden würde mich freuen.
Besonders übel wird's, wenn ein Foltermord mit Atomterrorismus und Landesspionage kombiniert wird.
www.bookwriter0815.info
www.bookwriter0815.de
www.bookwriter0815.wordpress.com
DESIGN:RETAIL FORUM: In-Store Technology As Retailtainment: Bright Shiny Obje...Deborah Weinswig
Retailers have invested in a variety of digital store technologies ranging from interactive displays and virtual dressing rooms to in-store customization and smart shelves. As consumers increasingly turn to e-commerce, retailers must create a unique in-store experience to maintain footfall. This presentation will address the following questions: What will be the overall potential impact of implementing these technologies? What are the challenges of implementing such technologies? And what is the impact on customer experiences in both the short and long term?
Want To Open A Small Business? Move To One Of These 25 Cities.When I Work
Looking to launch your next venture in a business-friendly climate? If you’re lucky enough to live near one of the following communities, you’re in luck. If not, consider whether a move will give your business the best odds possible of succeeding.
Here are the 25 cities worth moving to if you want to launch a startup:
MBAUniverse.com presents a complete guide on Group Discussion Topics and Ideas with Answers, to help students preparing for GD, GD Tips, Group Discussion Topics, GD Ideas, Group Discussion Preparation
Biotechnology is the utilization of biology to figure out problems and develop beneficial products. The most important area of biotechnology is the manufacturing of therapeutic proteins and other drugs via genetic engineering.
Genetic Engineering, also called as recombinant DNA technology, involves the group of techniques used to cut up and join together genetic material, especially DNA from different biological species, and to introduce the resulting hybrid DNA into an organism in order to form new combinations of heritable genetic material. This slide will illustrate the basic concepts and steps involved in Genetic Engineering.
This presentation is all about biotechnology. It is about the basic aspects of Biotechnology and covers a lot of topics under biotechnology, recombinant DNA technology. This is specifically for the HSC students of Mumbai. I hope that it helps.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
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CHAPTER- 11
BIOTECHNOLOGY: PRINCIPLES AND PROCESSES
The techniques of using live organisms or enzymes from organisms to produce products and
processes useful to humans. Many processes like in vitro fertilization leading to ‘test-tube’
baby, synthesizing gene and using it, developing a DNA vaccine or correcting a defective
gene are also parts of Biotechnology.
The European Federation of Biotechnology (EFB) definition of biotechnology is as follow-
“the integration of natural science and organisms, cells, parts thereof, and molecular
analogous for products and services”.
Principles of Biotechnology
Modern biotechnology is based on two main principles-
• Genetic Engineering- it is the technique of altering the chemistry of genetic material
(DNA and RNA) to introduce these into host organisms and thus changes the
phenotype of the host organism.
• Maintenance of sterile condition in chemical engineering process to enable growth of
only desired microbes for manufacture of biotechnological products like antibiotics,
vaccine, enzymes etc.
• Traditional hybridization used in plants and animal breeding leads to inclusion and
multiplication of undesirable genes along with the desired traits. The technique of
genetic engineering which include creation of recombinant DNA, use of gene cloning
and gene transfer allow us to isolate and introduce only one or a set of desirable genes
without introducing undesirable genes into the target organism.
In chromosome there is a specific DNA sequence called the origin of replication, which
is responsible for initiating replication. In genetic engineering the foreign DNA is linked
with the origin of replication, so the foreign DNA can replicate and multiply itself in the
host organism, which is also known as cloning or making multiple identical copies of
any template DNA.
The construction of artificial recombinant DNA requires linking a gene encoding
antibiotic resistance with a native plasmid of Salmonella typhimurium.
Stanley Cohen and Herbert Boyer in 1972 isolated the antibiotic resistance gene by
cutting out a piece of DNA from a plasmid which was making multiple identical copies
of any template DNA. The cutting of DNA at specific locations became possible with the
discovery of the so-called molecular scissors’– restriction enzymes.
The cut piece of DNA was then linked with the plasmid DNA. These plasmid DNA act as
vectors to transfer the piece of DNA attached to it.
The linking of antibiotic gene with the plasmid vector become possible with the
enzyme ligase, which acts on cut DNA molecules and joins their ends. This makes a new
combination of autonomously replicating DNA created in vitro and known as
recombinant DNA.
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When this DNA is transferred into E.coli, it could replicate using the new host DNA
polymerase enzyme and make multiple copies. The ability to multiply copies of
antibiotic resistance gene in E.coli was called cloning of antibiotic resistance gene in
E.coli.
Steps of Genetically Modifying an organism-
I. Identification of DNA with desirable genes.
II. Introduction of the identified DNA into the host.
III. Maintenance of introduced DNA in the host and transfer of the DNA to its progeny.
Tools of Recombinant DNA Technology includes
• Restriction Enzymes
• Polymerase enzymes
• Ligases
• Vectors host organisms
Restriction enzymes are responsible for restricting the growth of bacteriophage in
E.coli was called as restriction endonuclease. The first restriction endonuclease-
Hind II always cut DNA molecule at a particular point by recognizing a specific
sequence of six base pairs, called recognition sequence. Restriction enzymes belong
to group of enzymes called nucleases.
Each restriction endonuclease recognises a specific palindromic nucleotide sequence in
the DNA. Palindromes are group of letters that form the same words when read both
forward and backward for example “MALYALAM”.
The palindrome in DNA is a sequence of base pairs that reads same on two stands when
orientation of reading is kept the same.
Nucleases
Exonucleases
remove nucleotides
from ends of DNA
Endsonucleases
cuts at the specific position
withing the DNA
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Restriction enzymes cut the strand of DNA a little away from the centre of the
palindrome site between the same two bases on the opposite strands having sticky
strand. The stickiness of the strands facilities the action of the enzyme DNA ligase.
Restriction endonucleases are used in genetic engineering to form recombinant
molecules of DNA which are composed of DNA from different sources.
When cut the same restriction enzyme the resultant DNA fragments have the same kind
of Sticky-ends and can be joined together using DNA ligases.
Diagrammatic representation of Recombinant DNA technology
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Separation and isolation of DNA fragments
The fragment of DNA obtained by cutting DNA using
restriction enzyme is separated by technique called
gel electrophoresis. Negatively charged DNA
fragments can be separated by forcing them to move
towards the anode under an electric field through
medium. DNA fragments separate according to their
size through sieving effect provided by agarose gel.
• The separated DNA fragment can be visualized after staining the DNA with ethodium
bromide followed by exposure to UV light. Separated bands of DNA are separated from
agarose gel and extracted from gel, called elution. The DNA fragment purified this way
is used for recombination.
Cloning Vector
Plasmids and Bacteriophages is commonly used vector for cloning. They have ability to
replicate within bacterial cells independent of the control of chromosomal DNA.
Following features are required to facilitate cloning into a vector-
a. Origin of replication (ori) – the sequence from where replication starts and any
piece of DNA when linked to this sequence can be made to replicate within the host
cells.
b. Selectable marker-help in the identifying and eliminating nontransformants and
selectively permitting the growth of the
transformants. A piece of DNA is introduced in a host
bacterium through transformation procedure.
c. Cloning sites- to link the foreign DNA, the vector
need to have single recognition sites for the
commonly used restriction enzymes. The ligation of
foreign DNA is carried out at a restriction site present
in one of the two antibiotic resistance genes.
d. Vectors for cloning genes in plants and animals-
agrobacterium tumefactions (pathogen of dicot plant) is able to deliver a piece of DNA
known as ‘T-DNA” to transform normal plant cells into a tumor and direct these tumor
cells to produce the chemicals required by the pathogen. Retroviruses in animals have
the ability to transform normal cells into cancerous cells. The tumor inducing (Ti)
plasmid of Agrobacterium tumefaciens has been modified into cloning vector having no
more pathogenic to plant. Similarly retrovirus have been modified into cloning vector
for animals.
Competent host
DNA molecules are impermeable through Bacterial cell to take by plasmid due to
hydrophilic nature. Bacterial cell is made competent to take up DNA by treating with
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specific concentration of divalent cations (calcium) to increase the efficiency to enter
DNA into bacterium. This is followed by rapid heat and cold treatment.
• Now a day, DNA is directly introduced into host cell by microinjection in which DNA is
directly injected into the nucleus of an animal cell. Biolistic or gene gun is also used to
inject DNA in to target host.
Processed of Recombinant DNA Technology
Recombinant DNA technology involves several steps in specific sequence-
a. Isolation of DNA
b. Fragmentation of DNA by restriction endonucleases
c. Isolation of a desired DNA fragment
d. Ligation of the DNA fragment into vector
e. Transforming the recombinant DNA into the host
f. Culturing the host cells in a medium at large scale
g. Extraction of the desired product.
Genetic material is isolated from other macromolecules by using enzymes like lysozyme,
cellulose, chitinase etc. DNA that separate out can be removed by spooling.
Cutting of DNA at specific location is performed by using restriction enzyme and
Agarose gel electrophoresis to check the progression of a restriction enzyme digestion.
After cutting sources of DNA as well as vector DNA with a specific restriction enzyme to
cut out ‘gene of interest’ from he source DNA.
Amplification of Gene of Interest using PCR( Polymerase Chain Reaction) to get
multiple copies of the DNA or gene of interest in vitro by using set of primers and
enzyme DNA polymerase.
Insertion of Recombinant DNA into the Host Cell/Organism includes making the
recipient cells competent to receive, take up DNA present in its surrounding etc. The
recombinant DNA bearing gene for resistance to an antibiotic is transferred into E.coli
cells, the host cell become transformed into ampicillin-resistance cells.
Obtaining the foreign gene product – the foreign DNA multiplies in plant or animal
cell to produce desirable protein. Expression of foreign genes in host cells involve,
optimized condition to obtain recombinant protein. The recombinant cell is multiplied
in a continuous culture system in which used medium is drained out from one side while
fresh medium is added from the other to maintain the cells in their physiological active
phase. Bioreactors are used to obtain product on large scale at optimum temperature,
pressure and pH.
Downstream Processing involves processes that make the product obtain ready for
marketing. This process includes separation and purification called as downstream
processing. Suitable preservatives are added to it and send for clinical trial in case of
drugs before releasing to market for public use.