This antibody-cytokine fusion protein was achieved by conjugating/fusing the Anti-CD3 IgG to TNF. It was expressed in CHO and purified with affinity chromatography. The immunocytokine retains the ability to bind the CD3 as well as the biological activity of TNF. TNF-FuP provided a weak stimulus for lymphocyte proliferation, but it had no effect on LAK cells. However, it supported activation of Mfs and, to a minor extent, of CTLs. This immunocytokine was designed for treating CD30-positive lymphoma.

1. cd3e protein
• This antibody-cytokine fusion protein was achieved by
conjugating/fusing the Anti-CD3 IgG to TNF. It was expressed in CHO
and purified with affinity chromatography. The immunocytokine
retains the ability to bind the CD3 as well as the biological activity of
TNF. TNF-FuP provided a weak stimulus for lymphocyte proliferation,
but it had no effect on LAK cells. However, it supported activation of
Mfs and, to a minor extent, of CTLs. This immunocytokine was
designed for treating CD30-positive lymphoma.

2. • Specifications
• TargetCD3
• Molecule ClassTNF-IgGa
• Species ReactivityHuman
• HostMouse
• Expression HostCHO
• CloneOKT3
• Related DiseaseCD30-positive lymphoma
• Cytokines 1TNF
• Cytokines 1 DescriptionTumor necrosis factor (TNF), formerly known as TNFα or TNF alpha, is the best-known member of this class. TNF is a monocyte-derived cytotoxin that has been implicated in tumor regression, septic shock, and cachexia.
The protein is synthesized as a prohormone with an unusually long and atypical signal sequence, which is absent from the mature secreted cytokine. A short hydrophobic stretch of amino acids serves to anchor the prohormone in lipid bilayers.
Both the mature protein and a partially processed form of the hormone can be secreted after cleavage of the propeptide.
• Cytokine 1 SpeciesHuman
• ApplicationsThis product can be used therapeutically to deliver biologically active ligands to a desired tissue.
• Target
• Alternative NamesCD3E; CD3e molecule; epsilon (CD3-TCR complex); T3E; TCRE; IMD18; T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon
• Entrez Gene ID916
• UniProt IDP07766

3. • For lab research use only, not for diagnostic, therapeutic or any in
vivo human use.