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Recombinant vaccines
- 1. PRODUCTION OF RECOMBINANT VACCINES Present by:Sristi Rajput Roll No.: 201651 M.Sc. Biotechnology III Semester Central University of Haryana
- 2. INTRODUCTION ● Biological preparationthat provides active acquired immunity ● Stimulates body’s immune system ● First successful case of vaccination was performed by Edward Jenner in 1796
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- 5. RECOMBINANT VACCINE ● Generatedusing recombinant DNA technology ● Manufactured with the assistance of expression systems Escherichia coli Ease of bioprocessing and scale-up Strain engineering Lower cost Shorter production time
- 6. Recombinant Subunit Vaccines DNA Vaccines Usethe genetic material of the pathogen itself to immunize the individual Recombinant Vector Vaccines Genes that encode antigens isolated from a pathogen can be inserted into nonvirulent viruses or bacteria TYPES OFRECOMBINANTVACCINE Involves cloning of gene encoding immunogenic protein and its expression in bacterial, yeast or mammalian cell
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- 10. PRODUCTION OF ARECOMBINANT VACCINE Yeast Bioreactor Shaker Incubator BMMY YDP Broth YPD+Zeocin Petri Plakasi Clinical Studies Purification Filtration Preclinical Studies Formulation
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- 12. ● Humoral andT-helper cell response ● Safe ● Antigen can be made more immunogenic ● Inexpensive ADVANTAGES
- 13. ● No cytotoxicT-cell response ● Polysaccharide vaccines can not be made ● Usually multiple doses required ● Adjuvants are needed DISADVANTAGES
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