The document summarizes cell injury, adaptation, and death. It discusses various types of cell death including necrosis, apoptosis, necrapoptosis, and anoikis. Causes of cell injury include internal stresses and external factors. Cells can respond to injury through recovery, adaptation mechanisms like atrophy, hypertrophy, hyperplasia, metaplasia, and storage, or death. Adaptation responses aim to restore homeostasis. The stages of cell injury and mechanisms of apoptosis and necrosis are described in detail.
Difference between reversible and irreversible cell injury,Mechanism of cell ...Rukhshanda Ramzaan
Cell Injury: Any change resulting in loss of the ability to maintain the normal or adapted homeostatic state.
Agents that cause cell injury
• Hypoxia / Ischemia (loss of blood supply)
• Microbial
• Parasitic
• Chemical
• Physical
• Trauma
• Genetic
• Nutritious
• Environmental
Types of Cell injury
Reversible Cell Injury
Pathologic changes that can be reversed in mild cellular injury when the stimulus is removed. Cell injury is reversible only up to a certain point otherwise it will be irreversible.
Changes in reversible cell injury
Cellular Swelling: Due to accumulation of intracellular water and endoplasmic reticulum & mitochondria.
Clumping of chromatin.
Irreversible Cell injury
Pathologic changes that are permanent and cause cell death, they cannot be reversed to normal state.
Changes in irreversible cell injury
Irreversible injury is marked by severe mitochondrial vacuolization, extensive damage to plasma membranes, detachment of ribosomes from the granular endoplasmic reticulum (ER). Injury to lysosomal bodies leads to leakage of lysosomal enzymes into the cytoplasm and condensation, fragmentation and lysis of nuclei.
This is a presentation on the topic of Inflammation and repair, prepared by Dr Ashish Jawarkar, he is MD in pathology and a teacher at Parul institute of Medical sciences and research Vadodara.
Difference between reversible and irreversible cell injury,Mechanism of cell ...Rukhshanda Ramzaan
Cell Injury: Any change resulting in loss of the ability to maintain the normal or adapted homeostatic state.
Agents that cause cell injury
• Hypoxia / Ischemia (loss of blood supply)
• Microbial
• Parasitic
• Chemical
• Physical
• Trauma
• Genetic
• Nutritious
• Environmental
Types of Cell injury
Reversible Cell Injury
Pathologic changes that can be reversed in mild cellular injury when the stimulus is removed. Cell injury is reversible only up to a certain point otherwise it will be irreversible.
Changes in reversible cell injury
Cellular Swelling: Due to accumulation of intracellular water and endoplasmic reticulum & mitochondria.
Clumping of chromatin.
Irreversible Cell injury
Pathologic changes that are permanent and cause cell death, they cannot be reversed to normal state.
Changes in irreversible cell injury
Irreversible injury is marked by severe mitochondrial vacuolization, extensive damage to plasma membranes, detachment of ribosomes from the granular endoplasmic reticulum (ER). Injury to lysosomal bodies leads to leakage of lysosomal enzymes into the cytoplasm and condensation, fragmentation and lysis of nuclei.
This is a presentation on the topic of Inflammation and repair, prepared by Dr Ashish Jawarkar, he is MD in pathology and a teacher at Parul institute of Medical sciences and research Vadodara.
Hi! I am Komal Sankaran, M.Sc. Biotechnology (Pune University Gold Medalist, 2013), CSIR-NET SPM fellow (Jun- 2014, 4th rank), CSIR-NET- LS (Dec 2013, 2nd rank), DBT JRF category- I. Please contact if anyone is interested in Life Sciences CSIR-NET coaching in Pune (Khadki area).
Email- komalsan91@gmail.com
Apoptosis is a
-pathway of cell death that is
-induced by an internally regulated program
-in which cells destined to die activate intrinsic enzymes that --degrade the cells’ own nuclear DNA and also nuclear and cytoplasmic proteins
-With minimal host reaction.
Introduction
Definition
History
Evolution and origin of apoptosis
Significance
Purpose of apoptosis
Steps /process
Morphological and biochemical changes
Mechanism of apoptosis
Caspases
Regulation of apoptosis
Disorders of apoptosis
Application
Conclusion
Referances
UiPath Test Automation using UiPath Test Suite series, part 4DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 4. In this session, we will cover Test Manager overview along with SAP heatmap.
The UiPath Test Manager overview with SAP heatmap webinar offers a concise yet comprehensive exploration of the role of a Test Manager within SAP environments, coupled with the utilization of heatmaps for effective testing strategies.
Participants will gain insights into the responsibilities, challenges, and best practices associated with test management in SAP projects. Additionally, the webinar delves into the significance of heatmaps as a visual aid for identifying testing priorities, areas of risk, and resource allocation within SAP landscapes. Through this session, attendees can expect to enhance their understanding of test management principles while learning practical approaches to optimize testing processes in SAP environments using heatmap visualization techniques
What will you get from this session?
1. Insights into SAP testing best practices
2. Heatmap utilization for testing
3. Optimization of testing processes
4. Demo
Topics covered:
Execution from the test manager
Orchestrator execution result
Defect reporting
SAP heatmap example with demo
Speaker:
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
Connector Corner: Automate dynamic content and events by pushing a buttonDianaGray10
Here is something new! In our next Connector Corner webinar, we will demonstrate how you can use a single workflow to:
Create a campaign using Mailchimp with merge tags/fields
Send an interactive Slack channel message (using buttons)
Have the message received by managers and peers along with a test email for review
But there’s more:
In a second workflow supporting the same use case, you’ll see:
Your campaign sent to target colleagues for approval
If the “Approve” button is clicked, a Jira/Zendesk ticket is created for the marketing design team
But—if the “Reject” button is pushed, colleagues will be alerted via Slack message
Join us to learn more about this new, human-in-the-loop capability, brought to you by Integration Service connectors.
And...
Speakers:
Akshay Agnihotri, Product Manager
Charlie Greenberg, Host
State of ICS and IoT Cyber Threat Landscape Report 2024 previewPrayukth K V
The IoT and OT threat landscape report has been prepared by the Threat Research Team at Sectrio using data from Sectrio, cyber threat intelligence farming facilities spread across over 85 cities around the world. In addition, Sectrio also runs AI-based advanced threat and payload engagement facilities that serve as sinks to attract and engage sophisticated threat actors, and newer malware including new variants and latent threats that are at an earlier stage of development.
The latest edition of the OT/ICS and IoT security Threat Landscape Report 2024 also covers:
State of global ICS asset and network exposure
Sectoral targets and attacks as well as the cost of ransom
Global APT activity, AI usage, actor and tactic profiles, and implications
Rise in volumes of AI-powered cyberattacks
Major cyber events in 2024
Malware and malicious payload trends
Cyberattack types and targets
Vulnerability exploit attempts on CVEs
Attacks on counties – USA
Expansion of bot farms – how, where, and why
In-depth analysis of the cyber threat landscape across North America, South America, Europe, APAC, and the Middle East
Why are attacks on smart factories rising?
Cyber risk predictions
Axis of attacks – Europe
Systemic attacks in the Middle East
Download the full report from here:
https://sectrio.com/resources/ot-threat-landscape-reports/sectrio-releases-ot-ics-and-iot-security-threat-landscape-report-2024/
GraphRAG is All You need? LLM & Knowledge GraphGuy Korland
Guy Korland, CEO and Co-founder of FalkorDB, will review two articles on the integration of language models with knowledge graphs.
1. Unifying Large Language Models and Knowledge Graphs: A Roadmap.
https://arxiv.org/abs/2306.08302
2. Microsoft Research's GraphRAG paper and a review paper on various uses of knowledge graphs:
https://www.microsoft.com/en-us/research/blog/graphrag-unlocking-llm-discovery-on-narrative-private-data/
Kubernetes & AI - Beauty and the Beast !?! @KCD Istanbul 2024Tobias Schneck
As AI technology is pushing into IT I was wondering myself, as an “infrastructure container kubernetes guy”, how get this fancy AI technology get managed from an infrastructure operational view? Is it possible to apply our lovely cloud native principals as well? What benefit’s both technologies could bring to each other?
Let me take this questions and provide you a short journey through existing deployment models and use cases for AI software. On practical examples, we discuss what cloud/on-premise strategy we may need for applying it to our own infrastructure to get it to work from an enterprise perspective. I want to give an overview about infrastructure requirements and technologies, what could be beneficial or limiting your AI use cases in an enterprise environment. An interactive Demo will give you some insides, what approaches I got already working for real.
Dev Dives: Train smarter, not harder – active learning and UiPath LLMs for do...UiPathCommunity
💥 Speed, accuracy, and scaling – discover the superpowers of GenAI in action with UiPath Document Understanding and Communications Mining™:
See how to accelerate model training and optimize model performance with active learning
Learn about the latest enhancements to out-of-the-box document processing – with little to no training required
Get an exclusive demo of the new family of UiPath LLMs – GenAI models specialized for processing different types of documents and messages
This is a hands-on session specifically designed for automation developers and AI enthusiasts seeking to enhance their knowledge in leveraging the latest intelligent document processing capabilities offered by UiPath.
Speakers:
👨🏫 Andras Palfi, Senior Product Manager, UiPath
👩🏫 Lenka Dulovicova, Product Program Manager, UiPath
Accelerate your Kubernetes clusters with Varnish CachingThijs Feryn
A presentation about the usage and availability of Varnish on Kubernetes. This talk explores the capabilities of Varnish caching and shows how to use the Varnish Helm chart to deploy it to Kubernetes.
This presentation was delivered at K8SUG Singapore. See https://feryn.eu/presentations/accelerate-your-kubernetes-clusters-with-varnish-caching-k8sug-singapore-28-2024 for more details.
Essentials of Automations: Optimizing FME Workflows with ParametersSafe Software
Are you looking to streamline your workflows and boost your projects’ efficiency? Do you find yourself searching for ways to add flexibility and control over your FME workflows? If so, you’re in the right place.
Join us for an insightful dive into the world of FME parameters, a critical element in optimizing workflow efficiency. This webinar marks the beginning of our three-part “Essentials of Automation” series. This first webinar is designed to equip you with the knowledge and skills to utilize parameters effectively: enhancing the flexibility, maintainability, and user control of your FME projects.
Here’s what you’ll gain:
- Essentials of FME Parameters: Understand the pivotal role of parameters, including Reader/Writer, Transformer, User, and FME Flow categories. Discover how they are the key to unlocking automation and optimization within your workflows.
- Practical Applications in FME Form: Delve into key user parameter types including choice, connections, and file URLs. Allow users to control how a workflow runs, making your workflows more reusable. Learn to import values and deliver the best user experience for your workflows while enhancing accuracy.
- Optimization Strategies in FME Flow: Explore the creation and strategic deployment of parameters in FME Flow, including the use of deployment and geometry parameters, to maximize workflow efficiency.
- Pro Tips for Success: Gain insights on parameterizing connections and leveraging new features like Conditional Visibility for clarity and simplicity.
We’ll wrap up with a glimpse into future webinars, followed by a Q&A session to address your specific questions surrounding this topic.
Don’t miss this opportunity to elevate your FME expertise and drive your projects to new heights of efficiency.
JMeter webinar - integration with InfluxDB and GrafanaRTTS
Watch this recorded webinar about real-time monitoring of application performance. See how to integrate Apache JMeter, the open-source leader in performance testing, with InfluxDB, the open-source time-series database, and Grafana, the open-source analytics and visualization application.
In this webinar, we will review the benefits of leveraging InfluxDB and Grafana when executing load tests and demonstrate how these tools are used to visualize performance metrics.
Length: 30 minutes
Session Overview
-------------------------------------------
During this webinar, we will cover the following topics while demonstrating the integrations of JMeter, InfluxDB and Grafana:
- What out-of-the-box solutions are available for real-time monitoring JMeter tests?
- What are the benefits of integrating InfluxDB and Grafana into the load testing stack?
- Which features are provided by Grafana?
- Demonstration of InfluxDB and Grafana using a practice web application
To view the webinar recording, go to:
https://www.rttsweb.com/jmeter-integration-webinar
Securing your Kubernetes cluster_ a step-by-step guide to success !KatiaHIMEUR1
Today, after several years of existence, an extremely active community and an ultra-dynamic ecosystem, Kubernetes has established itself as the de facto standard in container orchestration. Thanks to a wide range of managed services, it has never been so easy to set up a ready-to-use Kubernetes cluster.
However, this ease of use means that the subject of security in Kubernetes is often left for later, or even neglected. This exposes companies to significant risks.
In this talk, I'll show you step-by-step how to secure your Kubernetes cluster for greater peace of mind and reliability.
1. LMP 300Y – Lecture 1
Cell injury, adaptation & death
Douglas M. Templeton, Ph.D., M.D.
doug.templeton@utoronto.ca
References
Kumar, Cotran & Robbins, Basic Pathology, 7th ed.Saunders, 2003.
Apoptosis - Nature, Oct. 12, 2000; Annu. Rev. Pharmacol. Toxicol., 42:259 (2002)
Some images from http://medlib.med.utah.edu/WebPath/webpath.html
2002/2003; revised 2005
2. Cell death -- good and bad
- good: development, T cell clones, cancer cells
- bad: tissue destruction, atrophy
Four terms:
Necrosis
Apoptosis
Necrapoptosis
Anoikis
7. “Cellular function is lost far before cell death occurs,
and the morphologic changes of cell injury (or death)
lag far behind both.”
8. "Even at the level of the light microscope, it is apparent
that cells exhibit a finite number of morphologic
reactions to a wide range of internal and external
environmental stresses."
"This … implies common biochemical and molecular
mechanisms responsible for cell adaptation and failure of
adaptation, or cell death."
9. Different cells show different sensitivities/thresholds.
Exam ples:
• Brain cells, heart cells susceptible to hypoxia and ischemia; liver cells
susceptible to chem injury
ical .
• Calf muscle tolerates 2-3 h of ischemia, cardiac muscle dies in 20-30
min.
• Highly differentiated surface epithelial cells of the respiratory tract more
susceptible to cigarette smoke than less differentiated basal epithelia.
• Nutritional status – glycogen-replete hepatocyte more resistant to
ischem than depleted one.
ia
10. • Hypoxia - Oxygen deficiency
• Ischemia - Impaired blood supply
(arterial or venous occlusion)
• Infarction - Area of necrosis due to ischemia
11. Some basic types of tissues
• Epithelium, endothelium
• Connective tissue, fibroblasts
• Muscle tissue – smooth, skeletal, cardiac
• Nervous tissue
• Blood and lymph
13. FOUR VULNERABLE SYSTEMS:
• Cell membrane integrity
• ATP generation / mitochondrial function
• Protein synthesis / enzyme function
• Genetic integrity
14. SIX GENERAL MECHANISMS:
• ATP depletion (ox/phos or glycolysis)
• Oxygen (i) – ischemia/hypoxia
• Oxygen (ii) – ROS
• Loss of Ca2+ homeostasis
• Plasma membrane integrity
• Mitochondrial damage
39. OVERVIEW
i) Atrophy
– decreased testosterone –> prostatic atrophy (apoptosis)
ii) Hypertrophy
– exercise / skeletal muscle; hypertension / cardiac myocyte
iii) Hyperplasia
– hyperthyroidism, effect of excess TSH on thyroid gland
iv) Metaplasia
– ciliated epithelium –> squamous epithelium in smoker.
(Point for argument: Is the myofibroblast a metaplastic cell?)
v) Storage
– Gaucher's disease (glucocerebrosidase),Haemochromatosis
(Fe), Fatty liver (EtOH)
40. Some terms in the histology of cell injury:
Fluid or fat accumulates in vacuoles – cloudy swelling / hydropic degeneration
e.g., disruption of ion transport/pumping (loss of ATP –> Na+/K+ ATPase,
oxidation of thiols on pumps, disorganization of membrane lipids, …)
Fat accumulation – fatty change
- fatty acid synthesizing/transporting cells (heart, liver, kidney)
- ER membrane damage, ↓FA oxid'n, ↑TG synth., ↓lipoprotein synth., …
Irreversible injury:
A cell may be irreversibly injured long before any changes are apparent in the microscope.
Coagulation necrosis – influx of water and ions, mitochondrial swelling, general loss
of membrane integrity, influx of Ca2+ (coagulation of proteins, activation of enzymes),
release of lysosomal enzymes (autolysis)
46. APOPTOSIS
Membrane blebbing, cell shrinkage, protein fragmentation, chromatin
condensation, DNA degradation, engulfment
- central role of caspases, cysteine proteases cleaving Asp-Xxx bond
- upstream (initiator) and downstream (effector) caspases
- may inactivate (e.g., lamins) or activate (e.g., nucleosomal nuclease) substrate
47. Apoptosis vs. Coagulation Necrosis
Apoptosis Necrosis
Stimulus Physiological (Developmental, Hyppoxia, Toxins
Atrophy, …)
Selected Pathological
Histology Single cells, shrinkage, chromatin Cell swelling, groups of cells,
condensation, apoptotic bodies tissue disruption
Organelles Intact Swelling of mitochondria & ER
Nucleus Chromatin condensation, inter- Disappearance,
nucleosomal breaks, laddering Random DNA breaks
(karyorrhexis) (karyolysis)
Outcome Phagocytosis of apoptotic bodies Inflammation, regeneration or
repair by fibrosis
49. Bcl-2 family members – balance between pro-apoptotic (e.g., Bax, Bak)
and anti (e.g., Bcl-2, Bcl-x) determines outcome.
Hydrophobic C-terminal domain localizes them to outer mitochondrial
membrane.
With other proteins, form channels to facilitate release of Cyt c.
Mitochondrial permeability transition pore – MPTP
50.
51.
52. Caspases are synthesized as inactive zymogen; pro-domain, p20,
and p10 domains. Activated by cleavage between p20 and p10, and pro-
domain and p20. Active as tetramer of 2 p10 and 2 p20 domains.
Three models for caspase activation.
i) caspase cascade, e.g. downstream effectors caspase-3, -6, -7
ii) induced proximity, e.g., on ligand binding CD95 receptors aggregate to form
signaling complexes, which through adapter proteins bring about high local
concentrations of procaspase-8
iii) association with a regulatory subunit, e.g., caspase-9 and Apaf-1
53.
54. DNA damage can initiate apoptosis.
Dual function of p53:
If damage detected, cell cycle arrest.
If damage not repaired, iniates apoptosis.
How is damage sensed? Proteins of the ATM (ataxia telangiectasia-
mutated) and DNA-PK contain DNA binding domains and protein
kinase activity. Both phosphorylate p53.
55. Signals for ingestion:
i) altered sugars recognized by lectins on macrophages
ii) Thrombospondin – secreted by macrophages, binds to
apoptotic cells (mechanism not known), then macrophage
integrins bind to thrombospondin.
iii) phosphatidyl serine (annexin V)
56. Apoptosis can be suppressed
• at the level of caspases
• at the level of the mitochondria
• by ionic control
57. QuickTime™ and a
GIF decompressor
are needed to see this picture.
58.
59.
60. Necrapoptosis – Lemasters, Am. J. Physiol. 276: G1-G6 (1999).
Cell balanced between apoptosis and necrosis depending on
production of ATP.
Anoikis – Frisch & Ruoslahti, Current Opin. Cell Biol. 9: 701-706 (1997).
"Homelessness". Apoptosis initiated by detachment of epithelial cell
from matrix.